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243-B3) inhibits Recombinant Mouse IL-4 (404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA). The ND50 is typically 0.01-0.05 µg/mL." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
243-B3) was serially diluted and captured by Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB643) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
243-B3) inhibition of Recombinant Mouse IL-4 (404-ML) induced proliferation in the HT-2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.100‑1.00 µg/mL." class="big_lightbox" target="_blank">
243-B3) was serially diluted and captured by Mouse Anti-TGF‑ beta 3 Monoclonal Antibody (Catalog # MAB643) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-TGF‑ beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-243-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
8420-B3). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human TGF‑ beta 3 (Catalog # 243-B3). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human TGF‑ beta 3 proteins are measured by their ability to inhibit the IL-4-dependent proliferation of Ht-2 mouse T cell. (orange, green, respectively). " class="big_lightbox" target="_blank">
341-BR) was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human TGF beta 3 (8420-B3) was measured at a concentration range between 0.488 pM and 1000 pM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=2.766 pM. (Biacore T200)." class="big_lightbox" target="_blank">
BT-TGFB3) grades of Recombinant Human TGF-beta 3 as measured by its ability to inhibit the IL-4-dependent proliferation ofHT‑2 mouse T cells (orange, green, red, respectively)." class="big_lightbox" target="_blank">
Transforming Growth Factor beta 3 (TGF beta 3) is a member of the TGF beta family of growth factors. The TGF beta polypeptides are multifunctional, capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. The multi-modal nature of TGF beta is seen in its ability to stimulate or inhibit cellular proliferation. In general, cells of mesenchymal origin appear to be stimulated by TGF beta whereas cells of epithelial or neuroectodermal origin are inhibited by the peptide.
![Neutralization TGF-beta 3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TGF-beta_3_AF-243-NA_Block_Neutralize_8663.jpg)
![Neutralization TGF-beta 3 Antibody (20724) [Unconjugated]](http://images.novusbio.com/images/antibody/TGF-beta_3_MAB243_Block_Neutralize_9095.jpg)
![N/A TGF-beta 3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_TGFbeta_3_DY243_ELISA_1828.jpg)
![Bioactivity TGF-beta 3 [Unconjugated]](http://images.novusbio.com/images/243-b3_recombinant-human-tgf-beta-3-protein-bioactivity-1342023172157.jpg)
![Bioactivity TGF-beta 3 [Unconjugated]](http://images.novusbio.com/fullsize/protein/8420-b3_recombinant-human-tgf-beta-3-cho-expressed-protein-bioactivity-272020134450.jpg)
![Bioactivity TGF-beta 3 [Unconjugated]](http://images.novusbio.com/images/bt-tgfb3-afl_recombinant-human-tgf-beta-3-protein-animal-free-bioactivity-94202618508.jpg)
![Immunohistochemistry TGF-beta 3 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af-243-na_tgf-beta-3-affinity-purified-polyclonal-ab-immunohistochemistry-4122020133749.jpg)
![Western Blot TGF-beta 3 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-243-na_tgf-beta-3-affinity-purified-polyclonal-ab-8120255531688.jpg)
![ELISA TGF-beta 3 Antibody [Unconjugated]](http://images.novusbio.com/images/af-243-na_tgf-beta-3-affinity-purified-polyclonal-ab-elisa-639201854774822290.jpg)
![Immunohistochemistry TGF-beta 3 Antibody (20724) [Unconjugated]](http://images.novusbio.com/images/antibody/TGFbeta_3_MAB243_Immunohistochemistry_17333.jpg)
![Western Blot TGF-beta 3 Antibody (20724) [Unconjugated]](http://images.novusbio.com/fullsize/mab243_tgf-beta-3-mab-clone-20724-8120255552889.jpg)
![Surface Plasmon Resonance (SPR) TGF-beta 3 [Unconjugated]](http://images.novusbio.com/images/8420-b3_recombinant-human-tgf-beta-3-cho-expressed-protein-surface-plasmon-resonance-spr-144202585151..jpg)
![Bioactivity TGF-beta 3 [Unconjugated]](http://images.novusbio.com/fullsize/bt-tgfb3-afl_recombinant-human-tgf-beta-3-protein-animal-free-bioactivity-2142026142455.jpg)
![SDS-Page TGF-beta 3 [Unconjugated]](http://images.novusbio.com/fullsize/bt-tgfb3-afl_recombinant-human-tgf-beta-3-protein-animal-free-sds-page-2142026145029.jpg)
