Desmoglein-2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Desmoglein-2 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein‑2 at approximately ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Desmoglein-2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-2
Met1-Gly608
Accession # CAA81226
Specificity
Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Desmoglein-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DSG2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 5 ug/mL
  • Western Blot 0.25 ug/mL
Publications
Read Publications using
AF947 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmoglein-2 Antibody [Unconjugated]

  • ARVC10
  • ARVD10
  • Cadherin family member 5
  • CDHF5
  • CDHF5MGC117034
  • CMD1BB
  • desmoglein 2
  • Desmoglein2
  • Desmoglein-2
  • DSG2
  • HDGC
  • MGC117036
  • MGC117037

Background

Desmoglein-2 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein-2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).

  1. Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
  2. Elias, P. et al. (2001) J. Cell Biol. 153:243.
  3. Arnemann, J. et al. (1992) Genomics 13:484.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desmoglein-2 Antibody (AF947)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Cynomolgus Monkey, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.


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ICC
(3)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Cynomolgus Monkey
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF947 Applications Species
KK Myo Min, D Rojas-Cana, D Penko, M DeNichilo, MP Cockshell, CB Ffrench, EJ Thompson, O Asplund, CJ Drogemulle, RB Prasad, L Groop, ST Grey, HE Thomas, T Loudovaris, TW Kay, MG Mahoney, CF Jessup, PT Coates, CS Bonder Desmoglein-2 is important for islet function and beta-cell survival Cell Death & Disease, 2022-10-29;13(10):911. 2022-10-29 [PMID: 36309486] (IHC, Mouse) IHC Mouse
Edward C.Y. Lee, Ryan E. Tyler, Derrick Johnson, Natalie Koh, Biauw Chi Ong, Shi Yin Foo et al. High frequency of anti-DSG 2 antibodies in post COVID-19 serum samples Journal of Molecular and Cellular Cardiology 2022-09-01 [PMID: 35764120]
Hongjie Wang, Audrey Germond, Chang Li, Sucheol Gil, Jiho Kim, Hans-Peter Kiem et al. In vivo HSC transduction in rhesus macaques with an HDAd5/3+ vector targeting desmoglein 2 and transiently overexpressing cxcr4 Blood Advances 2022-08-09 [PMID: 35679480]
J Kim, C Li, H Wang, S Kaviraj, S Singh, L Savergave, A Raghuwansh, S Gil, A Germond, A Baldessari, B Chen, S Roffler, P Fender, C Drescher, D Carter, A Lieber Translational development of a tumor junction opening technology Scientific Reports, 2022-05-11;12(1):7753. 2022-05-11 [PMID: 35562182] (IHC, Cynomolgus Monkey) IHC Cynomolgus Monkey
Kim J, Beidler P, Wang H et al. Desmoglein-2 as a prognostic and biomarker in ovarian cancer Cancer Biology & Therapy 2020-12-01 [PMID: 33218274]
Werner W. Franke, Lisa M. Domke, Yvette Dörflinger, Ralf Zimbelmann The cell–cell junctions of mammalian testes. III. Absence of an endothelial cell layer covering the peritubular wall of the seminiferous tubules—an immunocytochemical correction of a 50-year-old error in the literature Cell and Tissue Research 2020-01-01 [PMID: 31713729]
Fatma H. Al-Awadhi, Lilibeth A. Salvador, Brian K. Law, Valerie J. Paul, Hendrik Luesch Kempopeptin C, a Novel Marine-Derived Serine Protease Inhibitor Targeting Invasive Breast Cancer Marine Drugs 2017-09-16 [PMID: 28926939]
Charles Vragniau, Jens-Martin Hübner, Peter Beidler, Sucheol Gil, Kamola Saydaminova, Zhuo-Zhuang Lu et al. Studies on the Interaction of Tumor-Derived HD5 Alpha Defensins with Adenoviruses and Implications for Oncolytic Adenovirus Therapy Journal of Virology 2017-03-15 [PMID: 28077642]
Hongjie Wang, Corinne Ducournau, Kamola Saydaminova, Maximilian Richter, Roma Yumul, Martin Ho et al. Intracellular Signaling and Desmoglein 2 Shedding Triggered by Human Adenoviruses Ad3, Ad14, and Ad14P1 Journal of Virology 2015-11-01 [PMID: 26292319]
Maximilian Richter, Roma Yumul, Hongjie Wang, Kamola Saydaminova, Martin Ho, Drew May et al. Preclinical safety and efficacy studies with an affinity-enhanced epithelial junction opener and PEGylated liposomal doxorubicin Molecular Therapy - Methods & Clinical Development 2015-03-11 [PMID: 26029716]
Show All 14 Publications.

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Bioinformatics

Gene Symbol DSG2
Uniprot