Recombinant Human TGF-beta 2 Protein

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1 μg/lane of Recombinant Human TGF-beta 2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
Recombinant Human TGF-beta 2 (Catalog # 302-B2) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced cell proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.025-0.25 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TGF-beta 2 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.025-0.25 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human TGF-beta 2 protein
Ala303-Ser414
Accession #
N-terminal Sequence
Ala303
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE

12 kDa, reducing conditions
24 kDa, non-reducing conditions

Publications
Read Publications using
302-B2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 20 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 2 Protein

  • BSC-1 cell growth inhibitor
  • cetermin
  • Glioblastoma-derived T-cell suppressor factor
  • G-TSF
  • MGC116892
  • polyergin
  • TGFB2
  • TGFbeta 2
  • TGF-beta 2
  • TGF-beta2
  • TGF-beta-2
  • transforming growth factor beta-2
  • transforming growth factor, beta 2

Background

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

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302-B2
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Publications for TGF-beta 2 (302-B2)(47)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A.

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, WB Ctrl.


Filter By Application
Bioassay
(42)
ELISA Standard
(2)
In Vivo
(2)
WB Ctrl
(1)
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Human
(30)
Mouse
(7)
Rat
(3)
Canine
(3)
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(1)
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(3)
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Showing Publications 1 - 10 of 47. Show All 47 Publications.
Publications using 302-B2 Applications Species
J Nishida, K Miyazono, S Ehata Decreased TGFBR3/betaglycan expression enhances the metastatic abilities of renal cell carcinoma cells through TGF-?-dependent and -independent mechanisms Oncogene, 2018;0(0):. 2018 [PMID: 29391598] (Bioassay, Human) Bioassay Human
J Wu, X Chen, X Liu, S Huang, C He, B Chen, Y Liu Autophagy regulates TGF-?2-induced epithelial-mesenchymal transition in human retinal pigment epithelium cells Mol Med Rep, 2017;0(0):. 2017 [PMID: 29286127] (Bioassay, Human) Bioassay Human
JD Glover, KL Wells, F Matthäus, KJ Painter, W Ho, J Riddell, JA Johansson, MJ Ford, CAB Jahoda, V Klika, RL Mort, DJ Headon Hierarchical patterning modes orchestrate hair follicle morphogenesis PLoS Biol., 2017;15(7):e2002117. 2017 [PMID: 28700594] (Bioassay, Mouse) Bioassay Mouse
N Selvamurug, Z He, D Rifkin, B Dabovic, NC Partridge Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-? Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation Stem Cells Int, 2017;2017(0):2450327. 2017 [PMID: 28512472] (Bioassay, Human) Bioassay Human
E Ventura, M Weller, I Burghardt Cutting Edge: ERK1 Mediates the Autocrine Positive Feedback Loop of TGF-? and Furin in Glioma-Initiating Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28484053] (Bioassay, Human) Bioassay Human
A Futakuchi, T Inoue, T Fujimoto, U Kuroda, M Inoue-Moch, E Takahashi, S Ohira, H Tanihara Molecular Mechanisms Underlying the Filtration Bleb-Maintaining Effects of Suberoylanilide Hydroxamic Acid (SAHA) Invest. Ophthalmol. Vis. Sci., 2017;58(4):2421-2429. 2017 [PMID: 28448670] (Bioassay, Human) Bioassay Human
N Tellios, JC Belrose, AC Tokarewicz, C Hutnik, H Liu, A Leask, M Motolko, M Iijima, SK Parapuram TGF-? induces phosphorylation of phosphatase and tensin homolog: implications for fibrosis of the trabecular meshwork tissue in glaucoma Sci Rep, 2017;7(1):812. 2017 [PMID: 28400560] (Bioassay, Human) Bioassay Human
MC Wojciechow, L Mahmutovic, DY Shu, FJ Lovicu ERK1/2 signaling is required for the initiation but not progression of TGF?-induced lens epithelial to mesenchymal transition (EMT) Exp. Eye Res., 2017;159(0):98-113. 2017 [PMID: 28365272] (Bioassay, Human) Bioassay Human
BA Boswell, A Korol, JA West-Mays, LS Musil Dual function of TGF? in lens epithelial cell fate: implications for secondary cataract Mol. Biol. Cell, 2017;0(0):. 2017 [PMID: 28209733] (Bioassay, Chicken) Bioassay Chicken
MM Roe, S Swain, TA Sebrell, MA Sewell, MM Collins, BA Perrino, PD Smith, LE Smythies, D Bimczok Differential regulation of CD103 (�E integrin) expression in human dendritic cells by retinoic acid and Toll-like receptor ligands J. Leukoc. Biol, 2017;0(0):. 2017 [PMID: 28087652] (Bioassay, Human) Bioassay Human
Show All 47 Publications.

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Bioinformatics

Gene Symbol TGFB2
Entrez
Uniprot