Recombinant Human TGF-beta 2 Protein


1 μg/lane of Recombinant Human TGF-beta 2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
Recombinant Human TGF-beta 2 (Catalog # 302-B2) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced cell proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.025-0.25 ng/mL.

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Applications Bioactivity

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Recombinant Human TGF-beta 2 Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.025-0.25 ng/mL.
Mouse myeloma cell line, NS0-derived human TGF-beta 2 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
12.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

12 kDa, reducing conditions
24 kDa, non-reducing conditions

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302-B2 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 20 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 2 Protein

  • BSC-1 cell growth inhibitor
  • cetermin
  • Glioblastoma-derived T-cell suppressor factor
  • G-TSF
  • MGC116892
  • polyergin
  • TGFB2
  • TGFbeta 2
  • TGF-beta 2
  • TGF-beta2
  • TGF-beta-2
  • transforming growth factor beta-2
  • transforming growth factor, beta 2


TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

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Publications for TGF-beta 2 (302-B2)(57)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Western Blot.

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Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using 302-B2 Applications Species
H Yonemura, A Futakuchi, M Inoue-Moch, T Fujimoto, E Takahashi, H Tanihara, T Inoue DNA methyltransferase inhibitor suppresses fibrogenetic changes in human conjunctival fibroblasts Mol. Vis., 2019;25(0):382-390. 2019 [PMID: 31523116] (Bioassay, Human) Bioassay Human
M Rutgers, F Bach, L Vonk, M van Rijen, V Akrum, A van Boxtel, W Dhert, L Creemers PTH decreases in vitro human cartilage regeneration without affecting hypertrophic differentiation PLoS ONE, 2019;14(4):e0213483. 2019 [PMID: 30947269] (Bioassay, Human) Bioassay Human
H Feng, X Zhao, Q Guo, Y Feng, M Ma, W Guo, X Dong, C Deng, C Li, X Song, S Han, L Cao Autophagy resists EMT process to maintain retinal pigment epithelium homeostasis Int. J. Biol. Sci., 2019;15(3):507-521. 2019 [PMID: 30745838] (Bioassay, Human) Bioassay Human
M Kobayashi, K Tokuda, Y Kobayashi, C Yamashiro, SH Uchi, M Hatano, K Kimura Suppression of Epithelial-Mesenchymal Transition in Retinal Pigment Epithelial Cells by an MRTF-A Inhibitor Invest. Ophthalmol. Vis. Sci., 2019;60(2):528-537. 2019 [PMID: 30707754] (Bioassay, Human) Bioassay Human
C Ozek, RC Krolewski, SM Buchanan, LL Rubin Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice Sci Rep, 2018;8(1):17293. 2018 [PMID: 30470794] (Bioassay, Mouse) Bioassay Mouse
CG Abraham, MP Ludwig, Z Andrysik, A Pandey, M Joshi, MD Galbraith, KD Sullivan, JM Espinosa ?Np63? Suppresses TGFB2 Expression and RHOA Activity to Drive Cell Proliferation in Squamous Cell Carcinomas Cell Rep, 2018;24(12):3224-3236. 2018 [PMID: 30232004] (Bioassay, Human) Bioassay Human
M Inoue-Moch, T Inoue, S Kojima, A Futakuchi, T Fujimoto, S Sato-Ohira, U Tsutsumi, H Tanihara Interleukin-6-mediated trans-signaling inhibits transforming growth factor-? signaling in trabecular meshwork cells J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29752408] (Bioassay, Human) Bioassay Human
CC Tsai, SB Wu, HC Kau, YH Wei Essential role of connective tissue growth factor (CTGF) in transforming growth factor-?1 (TGF-?1)-induced myofibroblast transdifferentiation from Graves&#039; orbital fibroblasts Sci Rep, 2018;8(1):7276. 2018 [PMID: 29739987] (Bioassay, Human) Bioassay Human
JUG Wagner, E Chavakis, EM Rogg, M Muhly-Rein, SF Glaser, S Günther, D John, F Bonini, AM Zeiher, L Schaefer, MJ Hannocks, RA Boon, S Dimmeler Switch in Laminin β2 to Laminin β1 Isoforms During Aging Controls Endothelial Cell Functions Arterioscler. Thromb. Vasc. Biol., 2018;0(0):. 2018 [PMID: 29599141] (Cell Culture, Human) Cell Culture Human
U Tsutsumi-K, T Inoue, A Futakuchi, K Shobayashi, E Takahashi, S Kojima, M Inoue-Moch, T Fujimoto, H Tanihara Decreased MCP-1/CCR2 axis-mediated chemotactic effect of conjunctival fibroblasts after transdifferentiation into myofibroblasts Exp. Eye Res., 2018;170(0):76-80. 2018 [PMID: 29458061] (Bioassay, Human) Bioassay Human
Show All 57 Publications.

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Gene Symbol TGFB2