TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free

Flow Cytometry: TGF-alpha Antibody (tAb2) [NBP2-81082] - Flow-cytometry using the [NBP2-81082]. Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of tAb2 (NBP2-81082, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

• Reactivity: Hu
• Applications: DB, ELISA, Flow
• Clone: tAb2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free (NBP2-81082) is a recombinant monoclonal antibody validated for use in ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Biologically active hemocyanin-coupled human TGF-alpha.
• Specificity: tAb2 binds the N-terminal sequence of TGF-alpha, VVSHFND (with residues 4 to 7 being the minimal binding epitope).
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TGFA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Flow Cytometry
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. Human transforming growth factora (TGFa) consists of 50 amino acid residues with three disulphide bridges. It belongs to the family of epidermal growth factor related peptides and binds to EGFreceptor. It is involved in cell proliferation in healthy cells and is found in various cancer cells. Transfection of Fibroblasts with the TGFa gene induces tumor formation.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TGF-alpha Antibody (tAb2) - Chimeric - Azide and BSA Free

• protransforming growth factor alpha
• TFGA
• TGFA
• TGFalpha
• TGF-alpha
• transforming growth factor, alpha
• transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TGFA