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239-A) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TGF-a (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TGF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA). The ND50 is typically 0.15-0.75 µg/mL." class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">
239-A) was serially diluted and captured by Goat Anti-Human TGF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TGF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
239-A) was serially diluted and captured by Goat Anti-Human TGF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TGF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-239-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
![Neutralization TGF-alpha Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TGF-alpha_AF-239-NA_Block_Neutralize_8661.jpg)
![N/A TGF-alpha [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_TGFalpha_DTGA00_ELISA_843.jpg)
![N/A TGF-alpha [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_TGFalpha_DY239_ELISA_1820.jpg)
![SEC-MALS TGF-alpha [Unconjugated]](http://images.novusbio.com/fullsize/239-a_recombinant-human-tgf-alpha-protein-cf-sec-mals-2810202585510.jpg)
![Immunohistochemistry TGF-alpha Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TGF-alpha_AF-239-NA_Immunohistochemistry_6651.jpg)
![Immunohistochemistry TGF-alpha Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/TGF-alpha_AF-239-NA_Immunohistochemistry_9684.jpg)
![Western Blot TGF-alpha Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af-239-na_human-tgf-alpha-affinity-purified-polyclonal-ab-8220249504624.jpg)
![ELISA TGF-alpha Antibody [Unconjugated]](http://images.novusbio.com/images/af-239-na_human-tgf-alpha-affinity-purified-polyclonal-ab-elisa-639201776713475646.jpg)
![ELISA TGF-alpha Antibody [Unconjugated]](http://images.novusbio.com/images/af-239-na_human-tgf-alpha-affinity-purified-polyclonal-ab-elisa-639201854774467692.jpg)
![N/A TGF-alpha [HRP]](http://images.novusbio.com/fullsize/elisa/TGF-alpha_DTGA00_ELISA_192.jpg)
