Recombinant Human KGF/FGF-7 Protein


Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates cell proliferation of 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 6‑60 ng/mL.
Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes more

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Recombinant Human KGF/FGF-7 Protein Summary

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Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 6-60 ng/mL.
E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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251-KG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KGF/FGF-7 Protein

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • HBGF7
  • HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)


KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

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Publications for KGF/FGF-7 (251-KG)(83)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Fish - Prionace glauca (Blue Shark), Hamster.

We have publications tested in 3 applications: Bioassay, Cell Culture, Stimulation.

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Showing Publications 1 - 10 of 83. Show All 83 Publications.
Publications using 251-KG Applications Species
Frum, T;Hsu, PP;Hein, RFC;Conchola, AS;Zhang, CJ;Utter, OR;Anand, A;Zhang, Y;Clark, SG;Glass, I;Sexton, JZ;Spence, JR; Opposing roles for TGF?- and BMP-signaling during nascent alveolar differentiation in the developing human lung bioRxiv : the preprint server for biology 2023-05-05 [PMID: 37205521] (Bioassay, Human) Bioassay Human
Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW; A distal lung organoid model to study interstitial lung disease, viral infection and human lung development Nature protocols 2023-05-10 [PMID: 37165073] (Bioassay, Human) Bioassay Human
IM Leko, N Schrode, J Torres, M Pezet, TA Thimraj, KG Beaumont, HW Snoeck Generation and expansion of transitional lung organoids from human pluripotent stem cells bioRxiv : the preprint server for biology, 2023-02-01;0(0):. 2023-02-01 [PMID: 36778291] (Bioassay, Human) Bioassay Human
P Madrigal, S Deng, Y Feng, S Militi, KJ Goh, R Nibhani, R Grandy, A Osnato, D Ortmann, S Brown, S Pauklin Epigenetic and transcriptional regulations prime cell fate before division during human pluripotent stem cell differentiation Nature Communications, 2023-01-25;14(1):405. 2023-01-25 [PMID: 36697417] (Bioassay, Human) Bioassay Human
S Raad, A David, M Sagniez, B Paré, Z Orfi, NA Dumont, MA Smith, C Faure iPSCs derived from esophageal atresia patients reveal SOX2 dysregulation at the anterior foregut stage Disease Models & Mechanisms, 2022-11-28;0(0):. 2022-11-28 [PMID: 36317486] (Bioassay, Human) Bioassay Human
GM Jowett, E Read, LB Roberts, D Coman, M Vilà Gonzá, T Zabinski, U Niazi, R Reis, TJ Trieu, D Danovi, E Gentleman, L Vallier, MA Curtis, GM Lord, JF Neves Organoids capture tissue-specific innate lymphoid cell development in mice and humans Cell Reports, 2022-08-30;40(9):111281. 2022-08-30 [PMID: 36044863] (Bioassay, Mouse) Bioassay Mouse
HR Seo, HJ Han, Y Lee, YW Noh, SJ Cho, JH Kim Human Pluripotent Stem Cell-Derived Alveolar Organoid with Macrophages International Journal of Molecular Sciences, 2022-08-16;23(16):. 2022-08-16 [PMID: 36012471] (Cell Culture, Human) Cell Culture Human
RB Werder, T Liu, KM Abo, J Lindstrom-, C Villacorta, J Huang, A Hinds, N Boyer, E Bullitt, M Liesa, EK Silverman, DN Kotton, MH Cho, X Zhou, AA Wilson CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells Oncogene, 2022-07-13;8(28):eabo6566. 2022-07-13 [PMID: 35857525] (Bioassay, Human) Bioassay Human
V Lungova, K Wendt, SL Thibeault Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling Oncogene, 2022-08-23;0(0):. 2022-08-23 [PMID: 35770504] (Bioassay, Human) Bioassay Human
AK Nair, M Traurig, JR Sutherland, YL Muller, ED Grellinger, L Saporito, RG Nelson, C Bogardus, LJ Baier Generation of Isogenic hiPSCs with Targeted Edits at Multiple Intronic SNPs to Study the Effects of the Type 2 Diabetes Associated KCNQ1 Locus in American Indians Cells, 2022-04-25;11(9):. 2022-04-25 [PMID: 35563754] (Bioassay, Human) Bioassay Human
Show All 83 Publications.

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Gene Symbol FGF7