Recombinant Human KGF/FGF-7 Protein


Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates cell proliferation of 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 6‑60 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human KGF/FGF-7 Protein Summary

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Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 6-60 ng/mL.
The specific activity of recombinant human KGF/FGF-7 is approximately 1.3 x 103 U/μg, which is calibrated against recombinant human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).
E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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251-KG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KGF/FGF-7 Protein

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • HBGF7
  • HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)


KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

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Publications for KGF/FGF-7 (251-KG)(48)

We have publications tested in 4 confirmed species: Human, Mouse, Fish - Prionace glauca (Blue Shark), Hamster.

We have publications tested in 2 applications: Bioassay, Cell Culture.

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Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using 251-KG Applications Species
A Jacob, M Vedaie, DA Roberts, DC Thomas, C Villacorta, KD Alysandrat, F Hawkins, DN Kotton Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells Nat Protoc, 2019;0(0):. 2019 [PMID: 31732721] (Bioassay, Human) Bioassay Human
LS Beier, J Rossa, S Woodhouse, S Bergmann, HB Kramer, J Protze, M Eichner, A Piontek, S Vidal-Y-Sy, JM Brandner, G Krause, N Zitzmann, J Piontek Use of Modified Clostridium perfringens Enterotoxin Fragments for Claudin Targeting in Liver and Skin Cells Int J Mol Sci, 2019;20(19):. 2019 [PMID: 31561440] (Bioassay, Human) Bioassay Human
V Lungova, X Chen, Z Wang, C Kendziorsk, SL Thibeault Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure Nat Commun, 2019;10(1):4161. 2019 [PMID: 31551422] (Bioassay, Human) Bioassay Human
S Fujimoto, R Hayashi, S Hara, Y Sasamoto, J Harrington, M Tsujikawa, K Nishida KLF4 prevents epithelial to mesenchymal transition in human corneal epithelial cells via endogenous TGF- β2 suppression Regen Ther, 2019;11(0):249-257. 2019 [PMID: 31538102] (Bioassay, Human) Bioassay Human
K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019;28(2):382-393.e7. 2019 [PMID: 31291575] (Bioassay, Human) Bioassay Human
X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert Point mutations in the PDX1 transactivation domain impair human ?-cell development and function Mol Metab, 2019;0(0):. 2019 [PMID: 30930126] (Human) Human
L Liu, L Leng, C Liu, C Lu, Y Yuan, L Wu, F Gong, S Zhang, X Wei, M Wang, L Zhao, L Hu, J Wang, H Yang, S Zhu, F Chen, G Lu, Z Shang, G Lin An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos Nat Commun, 2019;10(1):364. 2019 [PMID: 30664750] (Bioassay, Human) Bioassay Human
AJ Miller, BR Dye, D Ferrer-Tor, DR Hill, AW Overeem, LD Shea, JR Spence Generation of lung organoids from human pluripotent stem cells in vitro Nat Protoc, 2019;14(2):518-540. 2019 [PMID: 30664680] (Bioassay, Human) Bioassay Human
MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA Cell Rep, 2018;25(13):3811-3827.e7. 2018 [PMID: 30590051] (Bioassay, Human) Bioassay Human
Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30244870] (Bioassay, Human) Bioassay Human
Show All 48 Publications.

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Gene Symbol FGF7