Recombinant Human KGF/FGF-7 Protein

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Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates cell proliferation of 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 6‑60 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human KGF/FGF-7 Protein Summary

Additional Information
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Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 6-60 ng/mL.
The specific activity of recombinant human KGF/FGF-7 is approximately 1.3 x 103 U/μg, which is calibrated against recombinant human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FGF7
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
251-KG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KGF/FGF-7 Protein

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • HBGF7
  • HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

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Publications for KGF/FGF-7 (251-KG)(57)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Fish - Prionace glauca (Blue Shark), Hamster.

We have publications tested in 2 applications: Bioassay, Cell Culture.


Filter By Application
Bioassay
(51)
Cell Culture
(3)
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Filter By Species
Human
(43)
Mouse
(9)
Rat
(1)
Fish - Prionace glauca (Blue Shark)
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 57. Show All 57 Publications.
Publications using 251-KG Applications Species
AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D Nature Protocols, 2021;0(0):. 2021 [PMID: 33649566] (Bioassay, Human) Bioassay Human
Y Ito, K Oshinden, N Kutsuzawa, C Kohno, S Isaki, K Yokoyama, T Sato, M Tanaka, K Asano Heat-Not-Burn cigarette induces oxidative stress response in primary rat alveolar epithelial cells PLoS ONE, 2020;15(11):e0242789. 2020 [PMID: 33237957] (Cell Culture, Rat) Cell Culture Rat
J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Lindstrom-, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response Cell Stem Cell, 2020;0(0):. 2020 [PMID: 32979316] (Bioassay, Human) Bioassay Human
AFM Dost, AL Moye, M Vedaie, LM Tran, E Fung, D Heinze, C Villacorta, J Huang, R Hekman, JH Kwan, BC Blum, SM Louie, SP Rowbotham, J Sainz de A, ME Piper, PJ Bhetariya, RT Bronson, A Emili, G Mostoslavs, GA Fishbein, WD Wallace, K Krysan, SM Dubinett, J Yanagawa, DN Kotton, CF Kim Organoids Model Transcriptional Hallmarks of Oncogenic KRAS Activation in Lung Epithelial Progenitor Cells Cell Stem Cell, 2020;27(4):663-678.e8. 2020 [PMID: 32891189] (Bioassay, Mouse) Bioassay Mouse
L Maddaluno, C Urwyler, T Rauschendo, M Meyer, D Stefanova, R Spörri, M Wietecha, L Ferrarese, D Stoycheva, D Bender, N Li, G Strittmatt, K Nasirujjam, HD Beer, P Staeheli, E Hildt, A Oxenius, S Werner Antagonism of interferon signaling by fibroblast growth factors promotes viral replication EMBO Mol Med, 2020;0(0):e11793. 2020 [PMID: 32720440] (Bioassay, Human) Bioassay Human
KA Ludwik, ZM Sandusky, KM Stauffer, Y Li, KL Boyd, GA O'Doherty, TP Stricker, DA Lannigan RSK2 Maintains Adult Estrogen Homeostasis by Inhibiting ERK1/2-Mediated Degradation of Estrogen Receptor Alpha Cell Rep, 2020;32(3):107931. 2020 [PMID: 32697984] (Bioassay, Mouse) Bioassay Mouse
J Huang, AJ Hume, KM Abo, RB Werder, C Villacorta, KD Alysandrat, ML Beermann, C Simone-Roa, J Olejnik, EL Suder, E Bullitt, A Hinds, A Sharma, M Bosmann, R Wang, F Hawkins, EJ Burks, M Saeed, AA Wilson, E Mühlberger, DN Kotton SARS-CoV-2 Infection of Pluripotent Stem Cell-derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response bioRxiv, 2020;0(0):. 2020 [PMID: 32637964] (Bioassay, Human) Bioassay Human
NK Vinckier, NA Patel, RJ Geusz, A Wang, J Wang, I Matta, AR Harrington, M Wortham, N Wetton, J Wang, US Jhala, MG Rosenfeld, CW Benner, HP Shih, M Sander LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development Nat Commun, 2020;11(1):2082. 2020 [PMID: 32350257] (Bioassay, Human) Bioassay Human
M Liu, Q Yan, Y Sun, Y Nam, L Hu, JH Loong, Q Ouyang, Y Zhang, HL Li, FE Kong, L Li, Y Li, MM Li, W Cheng, LX Jiang, S Fang, XD Yang, JQ Mo, YF Gong, YQ Tang, Y Li, YF Yuan, NF Ma, G Lin, S Ma, JG Wang, XY Guan A hepatocyte differentiation model reveals two subtypes of liver cancer with different oncofetal properties and therapeutic targets Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):. 2020 [PMID: 32123069] (Cell Culture, Human) Cell Culture Human
A Jacob, M Vedaie, DA Roberts, DC Thomas, C Villacorta, KD Alysandrat, F Hawkins, DN Kotton Derivation of self-renewing lung alveolar epithelial type II cells from human pluripotent stem cells Nat Protoc, 2019;0(0):. 2019 [PMID: 31732721] (Bioassay, Human) Bioassay Human
Show All 57 Publications.

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Bioinformatics

Gene Symbol FGF7
Uniprot