Recombinant Human KGF/FGF-7 Protein

Images

 
Recombinant Human KGF/FGF-7 (Catalog # 251-KG) stimulates cell proliferation of 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 6‑60 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Human KGF/FGF-7 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is typically 6-60 ng/mL.
The specific activity of recombinant human KGF/FGF-7 is approximately 1.3 x 103 U/μg, which is calibrated against recombinant human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).
Source
E. coli-derived human KGF/FGF-7 protein
Cys32-Thr194, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FGF7
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
251-KG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KGF/FGF-7 Protein

  • FGF7
  • FGF-7
  • fibroblast growth factor 7HBGF-7
  • HBGF7
  • HBGF-7
  • Heparin-binding growth factor 7
  • keratinocyte growth factor
  • KGF
  • KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha. (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively.

  1. Finch, P.W. and J.S. Rubin (2006) J. Natl. Cancer Inst. 98:812.
  2. Werner, S. et al. (2007) J. Invest. Dermatol. 127:998.
  3. Werner, S. (1998) Cytokine Growth Factor Rev. 9:153.
  4. Mason, I.J. et al. (1994) Mech. Dev. 45:15.
  5. Geer, D.J. et al. (2005) Am. J. Pathol. 167:1575.
  6. Niu, J. et al. (2007) J. Biol. Chem. 282:6001.
  7. Cardinali, G. et al. (2005) J. Invest. Dermatol. 125:1190.
  8. Qiao, J. et al. (1999) Development 126:547.
  9. Guo, L. et al. (1996) Genes Dev. 10:165.
  10. de Georgi, V. et al. (2007) Dermatol. Clin. 25:477.
  11. Hsu, Y-R. et al. (1999) Biochemistry 38:2523.
  12. Belleudi, F. et al. (2007) Traffic 8:1854.

Customers Who Viewed This Item Also Viewed...

NBP2-00932
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
7270-IL/CF
Species: Hu
NB110-68800
Species: Hu, Mu
Applications: WB, ICC/IF
NB600-1287
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IP, CyTOF-ready
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
AF1095
Species: Hu
Applications: WB, Simple Western, IHC, CyTOF-ready, ICC, ICFlow
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
251-KG
Species: Hu
Applications: Bioactivity

Publications for KGF/FGF-7 (251-KG)(43)

We have publications tested in 4 confirmed species: Human, Mouse, Fish - Prionace glauca (Blue Shark), Hamster.

We have publications tested in 2 applications: Bioassay, Cell Culture.


Filter By Application
Bioassay
(39)
Cell Culture
(1)
All Applications
Filter By Species
Human
(32)
Mouse
(7)
Fish - Prionace glauca (Blue Shark)
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 251-KG Applications Species
X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert Point mutations in the PDX1 transactivation domain impair human ?-cell development and function Mol Metab, 2019;0(0):. 2019 [PMID: 30930126] (Human) Human
L Liu, L Leng, C Liu, C Lu, Y Yuan, L Wu, F Gong, S Zhang, X Wei, M Wang, L Zhao, L Hu, J Wang, H Yang, S Zhu, F Chen, G Lu, Z Shang, G Lin An integrated chromatin accessibility and transcriptome landscape of human pre-implantation embryos Nat Commun, 2019;10(1):364. 2019 [PMID: 30664750] (Bioassay, Human) Bioassay Human
AJ Miller, BR Dye, D Ferrer-Tor, DR Hill, AW Overeem, LD Shea, JR Spence Generation of lung organoids from human pluripotent stem cells in vitro Nat Protoc, 2019;14(2):518-540. 2019 [PMID: 30664680] (Bioassay, Human) Bioassay Human
MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA Cell Rep, 2018;25(13):3811-3827.e7. 2018 [PMID: 30590051] (Bioassay, Human) Bioassay Human
Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30244870] (Bioassay, Human) Bioassay Human
JP Ng-Blichfe, A Schrik, RK Kortekaas, JA Noordhoek, IH Heijink, PS Hiemstra, J Stolk, M Königshoff, R Gosens Retinoic acid signaling balances adult distal lung epithelial progenitor cell growth and differentiation EBioMedicine, 2018;36(0):461-474. 2018 [PMID: 30236449] (Bioassay, Human) Bioassay Human
JM Kim, S Choi, SW Lee, K Park Voltage-dependent Ca2+ channels promote branching morphogenesis of salivary glands by patterning differential growth Sci Rep, 2018;8(1):7566. 2018 [PMID: 29765108] (Bioassay, Mouse) Bioassay Mouse
Y Ito, R Al Mubarak, N Roberts, K Correll, W Janssen, J Finigan, R Mishra, HW Chu IL-13 induces periostin and eotaxin expression in human primary alveolar epithelial cells: Comparison with paired airway epithelial cells PLoS ONE, 2018;13(4):e0196256. 2018 [PMID: 29672593] (Bioassay, Human) Bioassay Human
EJ Ostrin, DR Little, KN Gerner-Mau, EA Sumner, R Ríos-Corzo, E Ambrosio, SE Holt, NR Forcioli-C, H Akiyama, SM Hanash, S Kimura, SXL Huang, J Chen Beta-Catenin maintains lung epithelial progenitors after lung specification Development, 2018;0(0):. 2018 [PMID: 29440304] (Bioassay, Human) Bioassay Human
X Duan, M Xie, Y Peng, L Hu, J Yu, S Zeng, Y Wang, G Lu, G Lin, Y Sun Generation of a human embryonic stem cell line expressing tetrameric Zoanthus sp. green fluorescent protein: NERCe002-A-1 Stem Cell Res, 2018;28(0):6-10. 2018 [PMID: 29414419] (Bioassay, Human) Bioassay Human
Show All 43 Publications.

Reviews for KGF/FGF-7 (251-KG) (0)

There are no reviews for KGF/FGF-7 (251-KG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for KGF/FGF-7 (251-KG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional KGF/FGF-7 Products

Bioinformatics Tool for KGF/FGF-7 (251-KG)

Discover related pathways, diseases and genes to KGF/FGF-7 (251-KG). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for KGF/FGF-7 (251-KG)

Discover more about diseases related to KGF/FGF-7 (251-KG).
 

Pathways for KGF/FGF-7 (251-KG)

View related products by pathway.

PTMs for KGF/FGF-7 (251-KG)

Learn more about PTMs related to KGF/FGF-7 (251-KG).

Blogs on KGF/FGF-7

There are no specific blogs for KGF/FGF-7, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human KGF/FGF-7 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol FGF7
Uniprot