TDP-43/TARDBP Antibody


Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - Cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated more
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - Analysis using the DyLight 550 conjugate of NB110-55376. Staining of Human iPS derived neurons, fixed in 4% formaldehyde solution. The more
Western Blot: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in HeLa WCE.
Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody [NB110-55376] - TARDBP antibody was tested in MCF-7 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and more

Product Details

Reactivity Hu, Mu, Ch, Pm, Xp, ZeSpecies Glossary
Applications WB, ICC/IF, IHC
1 mg/ml

TDP-43/TARDBP Antibody Summary

A synthetic peptide to a C-terminal region [within residues 350-414] of the human TARDBP protein. [Swiss-Prot# Q13148]
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Glycine and 0.15M NaCl
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
Application Notes
This TARDBP antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis. In Western blot a band is seen at ~45 kDa, representing the human TARDBP protein. In ICC/IF a nuclear signal is present in MCF-7 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TDP-43/TARDBP Peptide (NB110-55376PEP)
Reviewed Applications
Read 1 Review rated 3
NB110-55376 in the following applications:

Read Publications using
NB110-55376 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • WB
    3 publications

Alternate Names for TDP-43/TARDBP Antibody

  • ALS10
  • ALS10TAR DNA-binding protein-43
  • TAR DNA binding protein
  • TDP43
  • TDP-43
  • TDP-43TAR DNA-binding protein 43


TARDBP (tar-DNA binding protein of 43) is a nucleic acids binding protein that regulates the process of transcription and splicing. Localized mainly in the nucleus, TARDBP is ubiquitously expressed with higher expression levels in pancreas, placenta, lung, genital tract and spleen, and has been suggested to implicate in microRNA biogenesis, cellular apoptosis as well as proliferation. TARDBP controls CFTR splicing and promotes CFTR exon 9 skipping by associating with UG repeated motifs in polymorphic region near 3'-splice site of this exon leading to aberrant splicing linked to pathological features typical of cystic fibrosis. TARDBP can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Moreover, it stabilizes the low molecular weight neurofilament mRNA through a direct interaction with the 3' UTR. TARDBP was originally identified as a major protein component of the ubiquitin immuno-positive inclusions which are the pathologic substrate of common forms of ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia), and later, it was found that TARDBP gene mutations are inherited in an autosomal dominant manner in familial/sporadic ALS. TARDBP has also been involved in other neurodegenerative such as Alzheimer's and Parkinson's disease.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TDP-43/TARDBP Antibody (NB110-55376)(10)

We have publications tested in 2 confirmed species: Human, Zebrafish.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

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Showing Publications 1 - 10 of 10.
Publications using NB110-55376 Applications Species
Goossens J, Vanmechelen E, Trojanowski JQ et al. TDP-43 as a possible biomarker for frontotemporal lobar degeneration: a systematic review of existing antibodies Acta Neuropathol Commun 2015 Apr 09 [PMID:25853864]
Li Q, Yokoshi M, Okada H, Kawahara Y. The cleavage pattern of TDP-43 determines its rate of clearance and cytotoxicity Nat Commun. 2015 Jan 29 [PMID:25630387]
Fang YS, Tsai KJ, Chang YJ et al. Full-length TDP-43 forms toxic amyloid oligomers that are present in frontotemporal lobar dementia-TDP patients. Nat Commun. 2014 Sep 13 [PMID:25215604]
Udan-Johns M, Bengoechea R, Bell S et al. Prion-like nuclear aggregation of TDP-43 during heat shock is regulated by HSP40/70 chaperones. Hum Mol Genet. 2013 Sep 4 [PMID:23962724] (WB, Human) WB Human
Schmid B, Hruscha A, Hogl S et al. Loss of ALS-associated TDP-43 in zebrafish causes muscle degeneration, vascular dysfunction, and reduced motor neuron axon outgrowth Proc Natl Acad Sci U S A 2013 Mar 26 [PMID:23457265] (WB, Zebrafish) WB Zebrafish
Kawahara Y, Mieda-Sato A. TDP-43 promotes microRNA biogenesis as a component of the Drosha and Dicer complexes. Proc Natl Acad Sci U S A;109(9):3347-52. 2012 Feb 28. [PMID:22323604]
Mitne-Neto M, Machado-Costa M, Marchetto MCN et al. Downregulation of VAPB expression in motor neurons derived from induced pluripotent stem-cells of ALS8 patients. Hum Mol Genet;20(18):3642-52. 2011 Sep 15. [PMID:21685205] (ICC/IF, Human) ICC/IF Human
Shiina Y, Arima K, Tabunoki H et al. TDP-43 dimerizes in human cells in culture. Cell Mol Neurobiol 2010 May [PMID:20043239] (WB, Human) WB Human
Wegorzewska I, Bell S, Cairns NJ et al. TDP-43 mutant transgenic mice develop features of ALS and frontotemporal lobar degeneration. Proc Natl Acad Sci U S A;106(44):18809-14. 2009 Nov 3. [PMID:19833869] (IHC, Human) IHC Human
Geser F et al. Evidence of multisystem disorder in whole-brain map of pathological TDP-43 in amyotrophic lateral sclerosis. Arch Neurol. 65(5):636-41. 2008 May. [PMID:18474740] (IHC, Human) IHC Human

Reviews for TDP-43/TARDBP Antibody (NB110-55376) (1) 31

Average Rating: 3
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB110-55376:
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Images Ratings Applications Species Date Details
Western Blot TDP-43/TARDBP NB110-55376
reviewed by:
WB Mouse 01/09/2015


ApplicationWestern Blot
Sample Testedmouse brain lysates


Blocking Details5% non-fat dry milk in 1x TBS, 4°C overnight

Primary Anitbody

Dilution Ratio1:500, 2 h RT, in 5% non-fat dry milk in 1x TBS

Secondary Antibody

Secondary DescriptionAnti-Rabbit IgG, Horse Radish Peroxidase (HRP) Linked
Secondary Manufacturer Cat#GE-Health Care NA934
Secondary Concentration1:5000


Detection NotesECL, 2 min exposure, positive control = SH5Y5 cells overexpr TDP-43, negative control = non-transgenic mouse


CommentsAntibody detects in non-transgenetic mouse and in transgenetic TDP-43 sample a fragment at 60 kDa. However, 45 kDa fragment is only detected in TDP-43 mouse sample. For a good signal exposure time must be at least 20 min.

Product General Protocols

View specific protocols for TDP-43/TARDBP Antibody (NB110-55376): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TDP-43/TARDBP Antibody (NB110-55376) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB110-55376AF405
Alexa Fluor (R) 488 Labeled NB110-55376AF488
Alexa Fluor (R) 647 Labeled NB110-55376AF647
Alexa Fluor (R) 700 Labeled NB110-55376AF700
Biotin Labeled NB110-55376B
DyLight 405 Labeled NB110-55376V
DyLight 488 Labeled NB110-55376G
DyLight 550 Labeled NB110-55376R
DyLight 650 Labeled NB110-55376C
FITC Labeled NB110-55376F
HRP Labeled NB110-55376H

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Contact Information

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Gene Symbol TARDBP

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