Western Blot: p97/VCP Antibody [NB100-1558] - Zymographic assay of gelatinase activity. Medium supernatants of 20ml culture medium (medium control) as well as 20 ml conditioned medium from 107 U937 cells, pMTH1-U937 ...read more
Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody ...read more
Western Blot: p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis ...read more
The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 806) of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
ICC/IF reactivity reported in (PMID: 23297223), IHC-Frozen reactivity reported in (PMID: 20833645).
89 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Zebrafish reactivity reported in scientific literature (PMID: 30010465). Mouse, Rat reactivity reported in scientific literature (PMID: 20833645).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for p97/VCP Antibody
15S Mg(2+)-ATPase p97 subunit
transitional endoplasmic reticulum ATPase
valosin containing protein
yeast Cdc48p homolog
Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for p97/VCP Antibody (NB100-1558). (Showing 1 - 1 of 1 FAQs).
I used the p97/VCP antibody (catalog # NB100-1558) and would now like to use for ICC. Is there a recommended dilution? In the Data Sheet can be found on the ICC not specified.
For the product NB100-1558, p97/VCP Antibody, the recommended starting dilution for ICC/IF is 1:100 to 1:500. Please note that we always advise customers to investigate the optimal dilution for them, as this will depend on the particular samples/reagents/protocols being used.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.