p97/VCP Antibody

Images

 
Western Blot: p97/VCP Antibody [NB100-1558] - Whole cell lysate (15 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiVCP antibody ...read more
p97/VCP Antibody [NB100-1558] - Detection of human VCP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ze, Po, BvSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

p97/VCP Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 750 and the C-terminus (residue 806) of human Valosin-Containing Protein using the numbering given in entry NP_009057.1 (GeneID 7415).
Predicted Species
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VCP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry-Frozen
  • Immunoprecipitation 1-4 ug/mg of lysate
Application Notes
ICC/IF was reported in scientific literature. IHC-Frozen reactivity reported in (PMID: 20833645).
Theoretical MW
89 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p97/VCP Overexpression Lysate
Publications
Read Publications using
NB100-1558 in the following applications:

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 30010465).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p97/VCP Antibody

  • 15S Mg(2+)-ATPase p97 subunit
  • IBMPFD
  • IBMPFDMGC8560
  • MGC131997
  • p97
  • TER ATPase
  • TERA
  • transitional endoplasmic reticulum ATPase
  • valosin containing protein
  • Valosin-containing proteinMGC148092
  • VCP
  • yeast Cdc48p homolog

Background

Valosin-containing protein (VCP or p97) belongs to the AAA (ATPase associated activities) family and acts as a molecular chaperone to a wide variety of cellular activities. Some of these activities include the alteration of both nuclear and mitotic golgi membranes, ubiquitin-proteasome dependent protein degradation, regulation of the NF-kappa b pathway, and extraction of membrane proteins. VCP has been shown to contain a substrate binding domain (N) and two conserved ATPase domains (D1 and D2). The three dimensional structure of VCP resembles that of a cylinder with the D1 and D2 stacked upon one another in a homo-hexameric ring formation. VCP is involved in the formation of neuronal inclusion bodies in neurodegenerative disease such as Parkinson's Disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p97/VCP Antibody (NB100-1558)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Zebrafish.

We have publications tested in 3 applications: ICC/IF, IHC-Fr, WB.


Filter By Application
ICC/IF
(1)
IHC-Fr
(1)
WB
(3)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
Rat
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB100-1558 Applications Species
Leto D, Morgens D, Zhang L et al. Parallel genome-wide CRISPR analysis identifies a role for heterotypic ubiquitin chains in ER-associated degradation bioRxiv Jun 17 2018 (WB, Human) WB Human
Leto DE, Morgens DW, Zhang L et al. Genome-wide CRISPR Analysis Identifies Substrate-Specific Conjugation Modules in ER-Associated Degradation Mol. Cell Dec 6 2018 [PMID: 30581143] (Human) Human
Kustermann M, Manta L, Paone C et al. Loss of the novel Vcp (valosin containing protein) interactor Washc4 interferes with autophagy-mediated proteostasis in striated muscle and leads to myopathy in vivo. Autophagy Aug 16 2018 [PMID: 30010465] (WB, Zebrafish) WB Zebrafish
Bersuker K, Peterson CWH, To M et al. A Proximity Labeling Strategy Provides Insights into the Composition and Dynamics of Lipid Droplet Proteomes. Dev. Cell 2017 Dec 19 [PMID: 29275994] (Human) Human
Regensburger M, Turk M, Pagenstecher A et al. VCP-related multisystem proteinopathy presenting as early-onset Parkinson disease Neurology 2017 Jul 19 [PMID: 28724584] (Human) Human
Olzmann JA, Richter CM, Kopito RR et al. Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover Proc Natl Acad Sci U S A 2013 Jan 7 [PMID: 23297223] (ICC/IF, Human) ICC/IF Human
Mandel K, Otte A, Hass R. Involvement of CD11b integrin in the alteration of metabolic factors after phorbol ester stimulation of human myeloid leukemia cells Cell Commun Signal 2012 Jul 11 [PMID: 22607136] (WB, Human) WB Human
Christianson JC, Olzmann JA, Shaler TA et al. Defining human ERAD networks through an integrative mapping strategy. Nature Cell Biology. 2011 Nov 27. [PMID: 22119785]
Clemen CS, Tangavelou K, Strucksberg KH et al. Strumpellin is a novel valosin-containing protein binding partner linking hereditary spastic paraplegia to protein aggregation diseases. Brain 2010 Oct [PMID: 20833645] (IHC-Fr, Rat, Mouse) IHC-Fr Rat, Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p97/VCP Antibody (NB100-1558). (Showing 1 - 1 of 1 FAQs).

  1. I used the p97/VCP antibody (catalog # NB100-1558) and would now like to use for ICC. Is there a recommended dilution? In the Data Sheet can be found on the ICC not specified.
    • For the product NB100-1558, p97/VCP Antibody, the recommended starting dilution for ICC/IF is 1:100 to 1:500. Please note that we always advise customers to investigate the optimal dilution for them, as this will depend on the particular samples/reagents/protocols being used.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VCP
Entrez
Uniprot