FUS Antibody

Images

 
Genetic Strategies: Knockout Validated: FUS Antibody [NB100-565] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. ...read more
Western Blot: FUS Antibody [NB100-565] - Whole cell lysate (50 ug) from HepG2, HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FUS antibody used for WB at ...read more
Immunohistochemistry-Paraffin: FUS Antibody [NB100-565] - FFPE section of mouse renal cells carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1ug/ml). Detection: DA
Immunohistochemistry-Paraffin: FUS Antibody [NB100-565] - FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: FUS Antibody [NB100-565] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antiFUS antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

FUS Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521).
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FUS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000-1:50000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Knockout Validated
Application Notes
Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
FUS Lysate (NBL1-10861)
FUS Lysate (NBP2-65872)
Reviewed Applications
Read 1 Review rated 5
using
NB100-565 in the following applications:

Publications
Read Publications using
NB100-565 in the following applications:

  • 1 publication
  • IHC
    3 publications
  • 1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 26403203).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FUS Antibody

  • 75 kDa DNA-pairing protein
  • ALS6
  • amyotrophic lateral sclerosis 6
  • FUS1
  • fused in sarcoma
  • fusion (involved in t(12;16) in malignant liposarcoma)
  • fusion gene in myxoid liposarcoma
  • fusion, derived from t(12;16) malignant liposarcoma
  • fus-like protein
  • heterogeneous nuclear ribonucleoprotein P2
  • HNRNPP2
  • Oncogene FUS
  • Oncogene TLS
  • POMP75
  • RNA-binding protein FUS
  • TLShnRNP-P2
  • Translocated in liposarcoma protein

Background

FUS is a nucleic acid-binding protein whose N-terminal half functions as a transcriptional activator domain in fusion oncoproteins found in human leukemias and liposarcomas. FUS has been reported to be associated with the nuclear matrix and has been implicated in transcription, pre-mRNA splicing processes, spliceosome assembly, and promoting undifferentiated endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FUS Antibody (NB100-565)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(3)
IHC-P
(1)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-565 Applications Species
Pansarasa O, Bordoni M, Dufruca L, Diamanti L. ALS lymphoblastoid cell lines as a considerable model to understand disease mechanisms. Dis Model Mech. 2018 Jan 29 [PMID: 29419416] (Human) Human
Prpar Mihevc S, Pavlin M, Darovic S et al. Modelling FUS Mislocalisation in an In Vitro Model of Innervated Human Muscle J. Mol. Neurosci. 2017 Jun 15 [PMID: 28620838] (IHC-P, Human) IHC-P Human
Lojk J, Prpar Mihevc S, Bregar VB et al. The Effect of Different Types of Nanoparticles on FUS and TDP-43 Solubility and Subcellular Localization. Neurotox Res. Apr 25 2017 [PMID: 28444573] (ICC/IF) ICC/IF
Gami-Patel P, Bandopadhyay R, Brelstaff J et al. The presence of heterogeneous nuclear ribonucleoproteins in frontotemporal lobar degeneration with FUS positive inclusions Neurobiology of Aging Jul 15 2016 [PMID: 27500866] (WB, IHC, Human) WB, IHC Human
Stoica R, Paillusson S, Gomez-Suaga P et al. ALS/FTD-associated FUS activates GSK-3 Beta to disrupt the VAPB-PTPIP51 interaction and ER-mitochondria associations EMBO Rep. 2016 Jul 14 [PMID: 27418313]
Sibley CR, Emmett W, Blazquez L et al. Recursive splicing in long vertebrate genes. Nature. 2015 May 21 [PMID: 25970246] (Human) Human
Rogelj B, Easton LE, Bogu GK et al. Widespread binding of FUS along nascent RNA regulates alternative splicing in the brain Sci Rep 2012 [PMID: 22934129] (IP, Mouse) IP Mouse
Suzuki N, Kato S, Kato M et al. FUS/TLS-immunoreactive neuronal and glial cell inclusions increase with disease duration in familial amyotrophic lateral sclerosis with an R521C FUS/TLS mutation. J Neuropathol Exp Neurol 2012 Sep [PMID: 22878663]
Ghazi-Noori S, Froud KE, Mizielinska S et al. Progressive neuronal inclusion formation and axonal degeneration in CHMP2B mutant transgenic mice Brain 2012 Mar [PMID: 22366797] (IHC, Mouse) IHC Mouse
Ahmed Z, Doherty KM, Silveira-Moriyama L et al. Globular glial tauopathies (GGT) presenting with motor neuron disease or frontotemporal dementia: an emerging group of 4-repeat tauopathies Acta Neuropathol 2011 Oct [PMID: 21773886] (IHC, Human) IHC Human
Show All 15 Publications.

Review for FUS Antibody (NB100-565) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-565:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence FUS NB100-565
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reviewed by:
Simona Darovic
IF Human 10/15/2013
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
LotA1
CommentsWe were very satisfied with Novus' anti-FUS antibody.We have also used this antibody in WB and it works very good. We used dilution 1:3000.

Blocking

Blocking Details3% BSA in PBS; 1h; room temperature

Primary Anitbody

Dilution RatioFUS NB100-565; Dilution: 1:400; 2h; room temperature

Secondary Antibody

Secondary DescriptionAlexa Fluor 555 goat anti-rabbit IgG (H+L)
Secondary Manufacturer Cat#Invitrogen A-21428
Secondary Concentration2 mg⁄ml; Dilution:1:1000

Details

Detection NotesFluorescence microscopy, AxioOserver.Z1, Exposure:200ms
Fixation DetailsFixation: 4%PFA in PBS 15 min RT; Permeabilization: 0,1%Triton in PBS 6 min RT
Wash DescriptionPBS, 3x5min; 1 mL

Comments

CommentsWe were very satisfied with Novus' anti-FUS antibody.We have also used this antibody in WB and it works very good. We used dilution 1:3000.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FUS Antibody (NB100-565) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for FUS Antibody (NB100-565)

Discover related pathways, diseases and genes to FUS Antibody (NB100-565). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FUS Antibody (NB100-565)

Discover more about diseases related to FUS Antibody (NB100-565).
 

Pathways for FUS Antibody (NB100-565)

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PTMs for FUS Antibody (NB100-565)

Learn more about PTMs related to FUS Antibody (NB100-565).

Blogs on FUS.

Transportin 1 and heterogeneous nuclear ribonucleoprotein D (hnRNPD)
Transportin 1, also known as Karyopherin- beta 2 or Importin- beta 2, is part of the beta -karyopherins family, which consists of importins and exportins responsible for the active transport of proteins between the nucleus and cytoplasm.  Transportin 1 is co...  Read full blog post.

Amyotrophic Lateral Sclerosis Infographic
Amyotrophic lateral sclerosis is a neurological disease which impacts motor neurons that are involved in muscle movement throughout the body. The progressive degeneration of neurons causes weakened muscles and can lead to paralysis. There is no cure f...  Read full blog post.

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Recent Reviews

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Simona Darovic
10/15/2013
Application: IF
Species: Human

Bioinformatics

Gene Symbol FUS
Entrez
Uniprot