TDP-43/TARDBP Overexpression Lysate (Native)

Images

 
Western Blot: TARDBP Overexpression Lysate (Adult Normal) [NBL1-16701] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TARDBP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TDP-43/TARDBP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC210639 and based on accession number NM_007375. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens TAR DNA binding protein (TARDBP), mRNA.
Gene
TARDBP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
44.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TDP-43/TARDBP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TDP-43/TARDBP Overexpression Lysate (Native)

  • ALS10
  • ALS10TAR DNA-binding protein-43
  • TAR DNA binding protein
  • TARDBP
  • TDP43
  • TDP-43
  • TDP-43TAR DNA-binding protein 43

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
NB100-565
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF1436
Species: Mu
Applications: WB
AF2420
Species: Hu
Applications: WB, IHC
NBP2-25162
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NBP1-88376
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB120-11433
Species: Hu, Mu, Rt, Ye
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
H00008878-M01
Species: Hu, Mu, Rt, Rb
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
MAB7509
Species: Hu
Applications: WB, IHC
NBP1-26380
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-85091
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-37399
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
H00002965-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P, RNAi
NBP2-02627
Species: Hu, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-19151
Species: Hu, Ma
Applications: WB, IHC-P, IP
NBP1-93504
Species: Hu
Applications: IHC, IHC-P
MAB25031
Species: Hu
Applications: WB, IHC, IP
NBP1-84682
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for TDP-43/TARDBP Lysate (NBL1-16701) (0)

There are no publications for TDP-43/TARDBP Lysate (NBL1-16701).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TDP-43/TARDBP Lysate (NBL1-16701) (0)

There are no reviews for TDP-43/TARDBP Lysate (NBL1-16701). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TDP-43/TARDBP Lysate (NBL1-16701) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TDP-43/TARDBP Products

Bioinformatics Tool for TDP-43/TARDBP Lysate (NBL1-16701)

Discover related pathways, diseases and genes to TDP-43/TARDBP Lysate (NBL1-16701). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TDP-43/TARDBP Lysate (NBL1-16701)

Discover more about diseases related to TDP-43/TARDBP Lysate (NBL1-16701).
 

Pathways for TDP-43/TARDBP Lysate (NBL1-16701)

View related products by pathway.

PTMs for TDP-43/TARDBP Lysate (NBL1-16701)

Learn more about PTMs related to TDP-43/TARDBP Lysate (NBL1-16701).
 

Research Areas for TDP-43/TARDBP Lysate (NBL1-16701)

Find related products by research area.

Blogs on TDP-43/TARDBP

There are no specific blogs for TDP-43/TARDBP, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TDP-43/TARDBP Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TARDBP
Entrez