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Optineurin Antibody

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Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).
Independent Antibodies: Western Blot: Optineurin Antibody [NBP1-84682] - Analysis using Anti-OPTN antibody NBP1-84682 (A) shows similar pattern to independent antibody NBP1-84683 (B).
Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [NBP1-84682] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining shown in green.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.
Orthogonal Strategies: Western Blot: Optineurin Antibody [NBP1-84682] - Analysis in human cell lines A-431 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant ...read more
Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

      

Orthogonal Strategies

  

Order Details

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Optineurin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKG
Specificity
Specificity of human Optineurin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPTN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.04-0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
WB reported in the literature (PMID:24943399). ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended.
Control Peptide
Optineurin Protein (NBP1-84682PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-84682 in the following applications:
Publications
Read Publication using
NBP1-84682 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24943399). Reactivity reported in mouse retina from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Optineurin Antibody

  • FIP2FIP-2
  • GLC1Eglaucoma 1, open angle, E (adult-onset)
  • HIP7ALS12
  • Huntingtin interacting protein L
  • Huntingtin yeast partner L
  • Huntingtin-interacting protein 7
  • Huntingtin-interacting protein L
  • HYPLHIP-7
  • NEMO-related protein
  • NRPE3-14.7K-interacting protein
  • Optic neuropathy-inducing protein
  • optineurin
  • TFIIIA-INTP
  • Transcription factor IIIA-interacting protein
  • transcrption factor IIIA-interacting protein
  • tumor necrosis factor alpha-inducible cellular protein containing leucinezipper domains

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Neuropilin‑1 was detected in immersion fixed paraffin-embedded sections of rat brain (thalamus) using Goat Anti-Mouse/Rat Neuropilin‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF566) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neuronal cell bodies and projections. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Bovine ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.
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Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.
Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (2E2-D3) [H00023435-M01] - TARDBP in rat tissue samples. Image courtsey of product review by Daniel Pirici.
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Immunocytochemistry/Immunofluorescence: TBK1 Antibody (108A429) [NB100-56705] - Antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). A dilution of 1:10 was used. Image objective 40x.
Immunohistochemistry-Paraffin: TBK1 Antibody (108A429) [NB100-56705] - Immunohistochemistry: TBK1 Antibody (108A429) - Azide and BSA Free [NBP2-33243] - Staining on human testis (Formalin-Fixed Paraffin-Embedded (FFPE)) Image from the Azide and BSA Free version of this antibody.
(1 Review)
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Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
(1 Review)
(4 Publications)
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Western Blot: MYO6 Antibody [NBP2-94213] - Analysis of extracts of various cell lines, using MYO6 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.
Immunocytochemistry/Immunofluorescence: MYO6 Antibody [NBP2-94213] - Analysis of HeLa cells using MYO6 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/Immunofluorescence: MYO6 Antibody [NBP2-94213] - Analysis of HeLa cells using MYO6 at dilution of 1:100. Blue: DAPI for nuclear staining.
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Applications: WB, ICC/IF
Optineurin Antibody
NBP1-84682
Optineurin Antibody
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Optineurin Antibody (NBP1-84682)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Reviews for Optineurin Antibody (NBP1-84682) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-84682:
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(1)
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(1)
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(2)
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Images Ratings Applications Species Date Details
Western Blot Optineurin NBP1-84682
Enlarge 		5
reviewed by:
Anonymous
 WB Mouse 02/20/2018
View

Summary

ApplicationWestern Blot
Sample TestedProtein Lysate
SpeciesMouse
LotC114003
Immunohistochemistry-Frozen Optineurin NBP1-84682
Enlarge 		5
reviewed by:
Anony- mous
 IHC-Fr Mouse 08/16/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesMouse
Special ApplicationsImage and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University.
FileView PDF

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Western Blot Optineurin NBP1-84682
Enlarge
 Anonymous
02/20/2018
Application: WB
Species: Mouse
read this review
Immunohistochemistry-Frozen Optineurin NBP1-84682
Enlarge
 Anony- mous
08/16/2014
Application: IHC-Fr
Species: Mouse
read this review

Bioinformatics

Gene Symbol OPTN