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Optineurin Antibody

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 (2 Reviews)
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Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant Tau 3. N2a cells + mutant tau + Drp1 full length + mutant Tau. This image was submitted via customer review.
Western Blot: Optineurin Antibody [NBP1-84682] - Effect of mitochondrial fission protein, Drp1 (FL) and mutant Tau on Optineurin expression in N2a (M) cells. Lanes: M. Ladder M. Ladder 1. N2a cells 2. N2a cells + mutant ...read more
Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [NBP1-84682] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol. Antibody staining shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining in human cerebral cortex and pancreas tissues. Corresponding OPTN RNA-seq data are presented for the same tissues.
Western Blot: Optineurin Antibody [NBP1-84682] - Analysis in human cell line HDLM-2.
Orthogonal Strategies: Western Blot: Optineurin Antibody [NBP1-84682] - Analysis in human cell lines A-431 and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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Optineurin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKG
Specificity
Specificity of human, mouse Optineurin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPTN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Frozen 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Frozen sections done on mouse retina from customer review.
Control Peptide
Optineurin Protein (NBP1-84682PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-84682 in the following applications:
Publications
Read Publication using
NBP1-84682 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24943399).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Optineurin Antibody

  • FIP2FIP-2
  • GLC1Eglaucoma 1, open angle, E (adult-onset)
  • HIP7ALS12
  • Huntingtin interacting protein L
  • Huntingtin yeast partner L
  • Huntingtin-interacting protein 7
  • Huntingtin-interacting protein L
  • HYPLHIP-7
  • NEMO-related protein
  • NRPE3-14.7K-interacting protein
  • Optic neuropathy-inducing protein
  • optineurin
  • TFIIIA-INTP
  • Transcription factor IIIA-interacting protein
  • transcrption factor IIIA-interacting protein
  • tumor necrosis factor alpha-inducible cellular protein containing leucinezipper domains

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.
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Western Blot: MYO6 Antibody [NBP2-30003] - MYO6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MYO6 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Immunocytochemistry/Immunofluorescence: MYO6 Antibody [NBP2-30003] - Confocal immunofluorescent analysis of (C-term R1181)(NBP2-30003) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Immunohistochemistry-Paraffin: MYO6 Antibody [NBP2-30003] - Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of (C-term R1181) for immunohistochemistry. Clinical relevance has not been evaluated.
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Publications for Optineurin Antibody (NBP1-84682)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Reviews for Optineurin Antibody (NBP1-84682) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-84682:
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Images Ratings Applications Species Date Details
Western Blot Optineurin NBP1-84682
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reviewed by:
Anonymous
 WB Mouse 02/20/2018
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Summary

ApplicationWestern Blot
Sample TestedProtein Lysate
SpeciesMouse
LotC114003
Immunohistochemistry-Frozen Optineurin NBP1-84682
Enlarge
reviewed by:
Anony- mous
 IHC-Fr Mouse 08/16/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesMouse
Special ApplicationsImage and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University.
FileView PDF

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Western Blot Optineurin NBP1-84682
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 Anonymous
02/20/2018
Application: WB
Species: Mouse
read this review
Immunohistochemistry-Frozen Optineurin NBP1-84682
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 Anony- mous
08/16/2014
Application: IHC-Fr
Species: Mouse
read this review

Bioinformatics

Gene Symbol OPTN