Optineurin Antibody

Images

 
Western Blot: Optineurin Antibody [NBP1-84682] - Analysis in human cell line HDLM-2.
Immunocytochemistry/ Immunofluorescence: Optineurin Antibody [NBP1-84682] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.
Immunohistochemistry-Frozen: Optineurin Antibody [NBP1-84682] - Image and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University. 20X and 100X.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: Optineurin Antibody [NBP1-84682] - Staining of human placenta shows moderate to strong cytoplasmic positivity in trophoblastic cells.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Optineurin Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:LGIVSELQLKLNSSGSSEDSFVEIRMAEGEAEGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLLCLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDEEKG
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OPTN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100. Frozen sections done on mouse retina from customer review.
Control Peptide
Optineurin Protein (NBP1-84682PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-84682 in the following applications:

Publications
Read Publication using
NBP1-84682 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24943399).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Optineurin Antibody

  • FIP2FIP-2
  • GLC1Eglaucoma 1, open angle, E (adult-onset)
  • HIP7ALS12
  • Huntingtin interacting protein L
  • Huntingtin yeast partner L
  • Huntingtin-interacting protein 7
  • Huntingtin-interacting protein L
  • HYPLHIP-7
  • NEMO-related protein
  • NRPE3-14.7K-interacting protein
  • Optic neuropathy-inducing protein
  • optineurin
  • TFIIIA-INTP
  • Transcription factor IIIA-interacting protein
  • transcrption factor IIIA-interacting protein
  • tumor necrosis factor alpha-inducible cellular protein containing leucinezipper domains

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Optineurin Antibody (NBP1-84682)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Review for Optineurin Antibody (NBP1-84682) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NBP1-84682:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Optineurin NBP1-84682
Enlarge
reviewed by:
Anony- mous
IHC-Fr Mouse 08/16/2014
View

Summary

ApplicationImmunohistochemistry-Frozen
SpeciesMouse
Special ApplicationsImage and review of Optineurin antibody on mouse retina (frozen sections) by Celia McKee, Duke University.
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Optineurin Antibody (NBP1-84682) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Optineurin Antibody (NBP1-84682)

Discover related pathways, diseases and genes to Optineurin Antibody (NBP1-84682). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

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1
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Anony- mous
08/16/2014
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol OPTN