CHMP2B Antibody (791521) [Unconjugated]

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CHMP2B Monoclonal Antibody (Catalog # MAB7509) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CHMP2B at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CHMP2B was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human CHMP2B Monoclonal Antibody (Catalog # MAB7509) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Staining is shown at both high (upper panel) and low (lower panel) magnification, and specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of U2OS human osteosarcoma cell line and CHMP2B knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 1.25 µg/mL of Mouse Anti-Human CHMP2B Monoclonal Antibody (Catalog # MAB7509) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for CHMP2B at approximately 28 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 791521
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CHMP2B Antibody (791521) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CHMP2B
  Ala2-Asp213
  Accession # Q9UQN3
• Specificity: Detects human CHMP2B in direct ELISAs and Western blots
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHMP2B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CHMP2B Antibody (791521) [Unconjugated]

• charged multivesicular body protein 2b
• CHMP2.5
• CHMP2.5VPS2BVPS2-2
• CHMP2B
• chromatin modifying protein 2B
• Chromatin-modifying protein 2b
• DKFZP564O123
• DMT1
• hVps2-2
• Vacuolar protein sorting-associated protein 2-2
• vacuolar protein-sorting-associated protein 2-2
• VPS2 homolog B
• Vps2-2
• VPS2B

Background

CHMP2B (CHarged Multivescular body Protein 2B; also Chromatin-Modifying Protein 2B and Vps2-2) is an approximately 35 kDa member of the SNF family of proteins. It is a cytosolic molecule that interacts with VPS4 and undergoes polymerization to form tubules that project plasma membrane outward, generating an environment conducive to membrane fission and budding. It also appears to participate in the formation of intraluminal vesicles associated with the lysosomal system. CHMP2B is found in striated muscle (heart and skeletal), and particularly in neurons of the CNS. Human CHMP2B is 213 amino acids (aa) in length. It contains one coiled-coil region (aa 25-55), an MIT (microtubule-interacting and trafficking) domain (aa 201-211) and a utilized phosphorylation site at Ser199. There is one alternative splice form that shows a deletion of aa 2-42. Full-length human CHMP2B shares 99% aa identity with mouse CHMP2B, but only 33% aa identity with human CHMP2A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHMP2B Antibody (MAB7509)(2)

Publications using MAB7509

• Alshafie, W;Fotouhi, M;Ayoubi, R;Shlaifer, I;Southern, K;McPherson, PS;Laflamme, C;NeuroSGC/YCharOS/EDDU collaborative group, ; The identification of high-performing antibodies for Charged multivesicular body protein 2b for use in Western Blot, immunoprecipitation and immunofluorescence F1000Research 2023-07-25 [PMID: 37635943] (Western Blot, Human)
• JR Cabrera, R Manivanh, BJ North, DA Leib The ESCRT-Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection MBio, 2019-03-05;10(2):. 2019-03-05 [PMID: 30837340] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CHMP2B
• Uniprot:
  • Q9UQN3(Human)