Progranulin/PGRN Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified ...read more
Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room ...read more
Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was ...read more
PGRN immunolabeling following ND-602.(A) Representative photomicrographs depicting PGRN immunolabeling in the dentate gyrus of the Tg2576 mouse brain following unilateral intracerebral administration of either LV-GFP, ...read more
PGRN immunolabeling following ND-602.(A) Representative photomicrographs depicting PGRN immunolabeling in the dentate gyrus of the Tg2576 mouse brain following unilateral intracerebral administration of either LV-GFP, ...read more
PGRN immunolabeling following ND-602.(A) Representative photomicrographs depicting PGRN immunolabeling in the dentate gyrus of the Tg2576 mouse brain following unilateral intracerebral administration of either LV-GFP, ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

Progranulin/PGRN Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Progranulin
Thr18-Leu589
Accession # P28798
Specificity
Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Grn
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2557 in the following applications:

Publications
Read Publications using
AF2557 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Progranulin/PGRN Antibody [Unconjugated]

  • Acrogranin
  • CLN11
  • GEP
  • GP88
  • granulin precursor
  • GRN
  • PCDGF
  • PEPI
  • PGRN
  • Proepithelin
  • Progranulin

Background

Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Progranulin/PGRN Antibody (AF2557)(31)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 8 applications: ELISA Development (Detection), ICC, IF, IHC, IHC-Fr, IHC-P, In Vivo, Western Blot.


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ELISA Development (Detection)
(1)
ICC
(4)
IF
(1)
IHC
(11)
IHC-Fr
(3)
IHC-P
(1)
In Vivo
(1)
Western Blot
(19)
All Applications
Filter By Species
Mouse
(30)
Rat
(1)
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using AF2557 Applications Species
He, Y;Kaya, I;Shariatgorji, R;Lundkvist, J;Wahlberg, LU;Nilsson, A;Mamula, D;Kehr, J;Zareba-Paslawska, J;Biverstål, H;Chergui, K;Zhang, X;Andren, PE;Svenningsson, P; Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents Nature communications 2023-09-19 [PMID: 37726325] (IHC, Mouse) IHC Mouse
Anderson, A;Tansey, MG; Loss of Progranulin Results in Increased Pan-Cathepsin Activity and Reduced LAMP1 Lysosomal Protein bioRxiv : the preprint server for biology 2023-07-16 [PMID: 37503267] (Western Blot, Mouse) Western Blot Mouse
Y He, X Zhang, I Flais, P Svenningss Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology International Journal of Molecular Sciences, 2022-10-10;23(19):. 2022-10-10 [PMID: 36233357] (IHC, Mouse) IHC Mouse
MN Santos, DH Paushter, T Zhang, X Wu, T Feng, J Lou, H Du, SM Becker, R Fragoza, H Yu, F Hu Progranulin-derived granulin E and lysosome membrane protein CD68 interact to reciprocally regulate their protein homeostasis The Journal of Biological Chemistry, 2022-08-04;0(0):102348. 2022-08-04 [PMID: 35933009] (Western Blot, Mouse) Western Blot Mouse
C Dedert, V Mishra, G Aggarwal, AD Nguyen, F Xu Progranulin Preserves Autophagy Flux and Mitochondrial Function in Rat Cortical Neurons Under High Glucose Stress Frontiers in Cellular Neuroscience, 2022-07-08;16(0):874258. 2022-07-08 [PMID: 35880011] (ICC, Western Blot, Rat) ICC, Western Blot Rat
T Zhang, H Du, MN Santos, X Wu, MD Pagan, LJ Trigiani, N Nishimura, T Reinheckel, F Hu Differential regulation of progranulin derived granulin peptides Molecular Neurodegeneration, 2022-02-04;17(1):15. 2022-02-04 [PMID: 35120524] (Western Blot, Mouse) Western Blot Mouse
K Takahashi, S Nakamura, W Otsu, M Shimazawa, H Hara Progranulin deficiency in Iba-1+ myeloid cells exacerbates choroidal neovascularization by perturbation of lysosomal function and abnormal inflammation Journal of Neuroinflammation, 2021-07-25;18(1):164. 2021-07-25 [PMID: 34304733] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
J Frew, HB Nygaard Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice Acta neuropathologica communications, 2021-04-01;9(1):57. 2021-04-01 [PMID: 33795008] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
M Murakoshi, T Gohda, E Adachi, S Ichikawa, S Hagiwara, Y Suzuki Differential organ-specific inflammatory response to progranulin in high-fat diet-fed mice Scientific Reports, 2021-01-13;11(1):1194. 2021-01-13 [PMID: 33441916] (IHC, Mouse) IHC Mouse
T Sasaki, M Shimazawa, H Kanamori, Y Yamada, A Nishinaka, Y Kuse, G Suzuki, T Masuda, S Nakamura, M Hosokawa, S Minatoguch, H Hara Effects of progranulin on the pathological conditions in experimental myocardial infarction model Sci Rep, 2020-07-16;10(1):11842. 2020-07-16 [PMID: 32678228] (IF, Western Blot, Mouse) IF, Western Blot Mouse
Show All 31 Publications.

Review for Progranulin/PGRN Antibody (AF2557) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2557:
Filter by Applications
Immunohistochemistry with free floating section
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Other Progranulin/PGRN AF2557
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4
reviewed by:
Verified Customer
Immunohistochemistry with free floating section Mouse 03/19/2018
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Summary

ApplicationOther
Sample TestedMouse brain
SpeciesMouse
LotUWR0117012

Comments

Commentsantigen retrieval for 15 mins, 1:250-1:500 dilution

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mFluor Violet Conjugated Antibodies

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Verified Customer
03/19/2018
Application: Immunohistochemistry with free floating section
Species: Mouse

Bioinformatics

Gene Symbol Grn
Uniprot