Progranulin/PGRN Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified ...read more
Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Progranulin/PGRN Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Progranulin
Thr18-Leu589
Accession # P28798
Specificity
Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
Grn
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2557 in the following applications:

Publications
Read Publications using
AF2557 in the following applications:

  • ICC
    1 publication
  • IHC
    2 publications
  • 1 publication
  • WB
    9 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Progranulin/PGRN Antibody [Unconjugated]

  • Acrogranin
  • CLN11
  • GEP
  • GP88
  • granulin precursor
  • GRN
  • PCDGF
  • PEPI
  • PGRN
  • Proepithelin
  • Progranulin

Background

Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Progranulin/PGRN Antibody (AF2557)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-F, WB.


Filter By Application
ICC
(1)
IHC
(2)
IHC-F
(1)
WB
(9)
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Filter By Species
Mouse
(13)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF2557 Applications Species
AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity Acta Neuropathol Commun, 2018;6(1):42. 2018 [PMID: 29855382] (WB,, Mouse) WB Mouse
K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology Nat Commun, 2018;9(1):433. 2018 [PMID: 29382817] (IHC,, Mouse) IHC Mouse
AE Arrant, VC Onyilo, DE Unger, ED Roberson Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis J. Neurosci., 2018;0(0):. 2018 [PMID: 29378861] (WB,, Mouse) WB Mouse
H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle J. Vet. Med. Sci., 2017;0(0):. 2017 [PMID: 29249750] (IHC-F,, Mouse) IHC-F Mouse
JM Van Kampen, DG Kay Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease PLoS ONE, 2017;12(8):e0182896. 2017 [PMID: 28837568] (WB,, Mouse) WB Mouse
X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu Lysosomal processing of progranulin Mol Neurodegener, 2017;12(1):62. 2017 [PMID: 28835281] (WB,, Mouse) WB Mouse
TL Petkau, J Blanco, BR Leavitt Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice Neurobiol. Dis., 2017;106(0):14-22. 2017 [PMID: 28647554] (IHC,, Mouse) IHC Mouse
Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina Sci Rep, 2016;6(0):23811. 2016 [PMID: 27030285] (WB,, Mouse) WB Mouse
Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells. Mol Med Rep, 2014;9(5):1675-80. 2014 [PMID: 24604062] (WB,, Mouse) WB Mouse
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts. Biochem Biophys Res Commun, 2012;430(1):419-24. 2012 [PMID: 23206705] (WB,, Mouse) WB Mouse
Show All 13 Publications.

Review for Progranulin/PGRN Antibody (AF2557) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2557:
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Images Ratings Applications Species Date Details
 Progranulin/PGRN AF2557
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reviewed by:
Anonymous
Mouse 03/19/2018
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Summary

Sample Tested Mouse brain
SpeciesMouse
LotUWR0117012

Comments

Commentsantigen retrieval for 15 mins, 1:250-1:500 dilution

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Anonymous
03/19/2018
Application:
Species: Mouse

Bioinformatics

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