Progranulin/PGRN Antibody [Unconjugated]

Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85‑90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Progranulin/PGRN Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Progranulin
  Thr18-Leu589
  Accession # P28798
• Specificity: Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Grn
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Progranulin/PGRN Antibody [Unconjugated]

• Acrogranin
• CLN11
• GEP
• GP88
• granulin precursor
• GRN
• PCDGF
• PEPI
• PGRN
• Proepithelin
• Progranulin

Background

Mouse Progranulin, also known as Proepithelin, PC Cell-derived Growth Factor and Acrogranin, is a secreted protein of 572 amino acid residues. It contains seven and one half granulin repeats which are characterized by 6 disulfide bridges. Progranulin can be converted in vivo to granulins by proteolysis. Progranulin is a mitogen that stimulates proliferation and survival of various epithelial, mesenchymal and tumor cells. Several granulins have also been shown to be biologically active and may play a role in inflammation and wound healing. Mouse Progranulin shares 86% and 75% amino acid sequence homology with rat and human Progranulin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Progranulin/PGRN Antibody (AF2557)(22)

Publications using AF2557

• T Sasaki, M Shimazawa, H Kanamori, Y Yamada, A Nishinaka, Y Kuse, G Suzuki, T Masuda, S Nakamura, M Hosokawa, S Minatoguch, H Hara Effects of progranulin on the pathological conditions in experimental myocardial infarction model Sci Rep, 2020;10(1):11842. 2020 [PMID: 32678228] (Western Blot, Mouse)
• FL Tang, L Zhao, Y Zhao, D Sun, XJ Zhu, L Mei, WC Xiong Coupling of terminal differentiation deficit with neurodegenerative pathology in Vps35-deficient pyramidal neurons Cell Death Differ., 2020;0(0):. 2020 [PMID: 31907392] (IHC, Mouse)
• C Wang, L Zhang, JC Ndong, A Hettinghou, G Sun, C Chen, C Zhang, R Liu, CJ Liu Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice J Neuroinflammation, 2019;16(1):238. 2019 [PMID: 31775776] (Western Blot, Mouse)
• F Wei, Z Jiang, H Sun, J Pu, Y Sun, M Wang, Q Tong, Y Bi, X Ma, GF Gao, J Liu Induction of PGRN by influenza virus inhibits the antiviral immune responses through downregulation of type I interferons signaling PLoS Pathog., 2019;15(10):e1008062. 2019 [PMID: 31585000] (In Vivo, Mouse)
• X Zhou, DH Paushter, MD Pagan, D Kim, M Nunez Sant, RL Lieberman, HS Overkleeft, Y Sun, MB Smolka, F Hu Progranulin deficiency leads to reduced glucocerebrosidase activity PLoS ONE, 2019;14(7):e0212382. 2019 [PMID: 31291241] (Western Blot, Mouse)
• LP Elia, AR Mason, A Alijagic, S Finkbeiner Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway J. Neurosci., 2019;0(0):. 2019 [PMID: 30696728] (ELISA Development (Detection), Mouse)
• Y Kuse, K Ohuchi, S Nakamura, H Hara, M Shimazawa Microglia increases the proliferation of retinal precursor cells during postnatal development Mol. Vis., 2018;24(0):536-545. 2018 [PMID: 30090016] (Western Blot, Mouse)
• AM Nicholson, X Zhou, RB Perkerson, TM Parsons, J Chew, M Brooks, M DeJesus-He, NA Finch, BJ Matchett, A Kurti, KR Jansen-Wes, E Perkerson, L Daughrity, M Castanedes, L Rousseau, V Phillips, F Hu, TF Gendron, ME Murray, DW Dickson, JD Fryer, L Petrucelli, R Rademakers Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity Acta Neuropathol Commun, 2018;6(1):42. 2018 [PMID: 29855382] (Western Blot, Mouse)
• N Uesaka, M Abe, K Konno, M Yamazaki, K Sakoori, T Watanabe, TH Kao, T Mikuni, M Watanabe, K Sakimura, M Kano Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum Neuron, 2018;0(0):. 2018 [PMID: 29398357] (IHC, Mouse)
• K Fujita, X Chen, H Homma, K Tagawa, M Amano, A Saito, S Imoto, H Akatsu, Y Hashizume, K Kaibuchi, S Miyano, H Okazawa Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology Nat Commun, 2018;9(1):433. 2018 [PMID: 29382817] (IHC, Mouse)
• AE Arrant, VC Onyilo, DE Unger, ED Roberson Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis J. Neurosci., 2018;0(0):. 2018 [PMID: 29378861] (Western Blot, Mouse)
• H Sugihara, K Miyaji, K Yamanouchi, T Matsuwaki, M Nishihara Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle J. Vet. Med. Sci., 2017;0(0):. 2017 [PMID: 29249750] (IHC, Mouse)
• JM Van Kampen, DG Kay Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease PLoS ONE, 2017;12(8):e0182896. 2017 [PMID: 28837568] (Western Blot, Mouse)
• X Zhou, DH Paushter, T Feng, L Sun, T Reinheckel, F Hu Lysosomal processing of progranulin Mol Neurodegener, 2017;12(1):62. 2017 [PMID: 28835281] (Western Blot, Mouse)
• TL Petkau, J Blanco, BR Leavitt Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice Neurobiol. Dis., 2017;106(0):14-22. 2017 [PMID: 28647554] (IHC, Mouse)
• S Beel, M Moisse, M Damme, L De Muynck, W Robberecht, L Van Den Bo, P Saftig, P Van Damme Progranulin functions as a cathepsin D chaperone to stimulate axonal outgrowth in vivo Hum. Mol. Genet., 2017;26(15):2850-2863. 2017 [PMID: 28453791] (ICC, Mouse)
• Y Kuse, K Tsuruma, S Sugitani, H Izawa, Y Ohno, M Shimazawa, H Hara Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina Sci Rep, 2016;6(0):23811. 2016 [PMID: 27030285] (Western Blot, Mouse)
• Luo L, Lu L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells. Mol Med Rep, 2014;9(5):1675-80. 2014 [PMID: 24604062] (Western Blot, Mouse)
• p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts. Biochem Biophys Res Commun, 2012;430(1):419-24. 2012 [PMID: 23206705] (Western Blot, Mouse)
• Gass J, Lee W, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link C, Rademakers R, Nykjaer A, Petrucelli L Progranulin regulates neuronal outgrowth independent of sortilin. Mol Neurodegener, 2012;7(0):33. 2012 [PMID: 22781549] (Western Blot, Mouse)
• Almeida S, Zhou L, Gao FB Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins. PLoS ONE, 2011;6(10):e26454. 2011 [PMID: 22028881] (ICC, Mouse)
• Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models. J. Pathol., 2009;219(2):173-81. 2009 [PMID: 19557827] (Western Blot, Mouse)

Review for Progranulin/PGRN Antibody (AF2557) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous Mouse 03/19/2018

Summary

• Sample Tested: Mouse brain
• Species: Mouse
• Lot: UWR0117012

Comments

• Comments: antigen retrieval for 15 mins, 1:250-1:500 dilution

Bioinformatics

• Gene Symbol: Grn
• Uniprot:
  • P28798(Mouse)