Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free

Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213] - Formalin-fixed, paraffin-embedded human brain stained with Synaptophysin Antibody (SYP/4503R).

Immunohistochemistry-Paraffin: Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free [NBP3-08213] - Formalin-fixed, paraffin-embedded human pancreas stained with Synaptophysin Recombinant Rabbit Monoclonal Antibody (SYP/4503R).

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: SYP/4503R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07325).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa 224-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary) (Uniprot: IDP08247)
• Localization: Cytoplasmic
• Marker: Neuroendocrine Marker
• Specificity: This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin. It is an N-glycosylated integral membrane protein found in neurons and endocrine cells. Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel. This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms. Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles. Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SYP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Synaptophysin Antibody (SYP/4503R) - Azide and BSA Free

• Major synaptic vesicle protein p38
• MRX
• MRXSYP
• Synaptophysin
• SYP

Background

Synaptophysin (Syp I), also called the major synaptic vesicle protein p38, is an integral membrane glycoprotein found in the synaptic vesicles of brain, neuron and neuroendocrine cells. Synaptophysin is one of the most abundant membrane proteins, comprising approximately 7% of the total protein in small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. Synaptophysin may also be used as a neuroendocrine tumor marker in both neuroscience and cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYP