Western Blot: STING/TMEM173 Antibody [NBP2-24548] - Analysis of STING in Rh30 lysate using STING antibody at 7 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this ...read more
The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a), chimpanzee and rhesus monkey, 87% homologous in dog and cow, 81% homologous in horse, mouse and boar and 75% homologous in rat.
STING (STimulator of INterferon Genes protein) acts as a sensor of cytosolic DNA from bacteria/viruses or the self (intrinsic) and promotes the production of type I interferons (IFN-alpha and IFN-beta). STING has been suggested to interact with DDX58/RIG-I, MAVS, SSR2, RNF5, TRIM56, TBK1, IFIT1 and IFIT2. It generally localize to the cytoplasm, and membranes of cell, ER and mitochondria but in response to DNA stimulation, it translocate to the perinuclear region for interaction with TBK1 kinase. The latter phosphorylates STING at Ser-358 residue which results in its activation. STING executes its role by spotting and binding cyclic di-GMP / c-di-GMP and cyclic GMP-AMP /cGAMP which follows the activation of NF-kappa B and IRF3 transcriptional signaling pathways leading to the induction of Type I interferon response. STING has also been suggested to involve in cell death signaling pathways through its association with MHC-II and the activation of ERK pathway. Besides immune response to bacterial/viral pathogens, innate immune gene transcription has been of great importance in the elucidation of the causes of auto-inflammatory disease involving the sensing of self-DNA and the generation of effective antitumor adaptive immunity. STING regulated innate signaling has offered critical insights and new opportunities into the development of novel immunization regimes and therapeutics for treatment of infection, autoimmune disorders, inflammation and cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.