Stabilin-1 Antibody [Unconjugated]

Human whole blood CD14+ monocytes were stained with Sheep Anti-Human Stabilin‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF3825, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Northern­Lights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Stabilin-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Stabilin-1
  Gly2208-Val2305 (Val2282Ile)
  Accession # Q9NY15
• Specificity: Detects human Stabilin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Stabilin-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: STAB1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Stabilin-1 Antibody [Unconjugated]

• CLEVER-1
• fasciclin egf-like, laminin-type egf-like, and link domain-containing scavengerreceptor-1
• Fasciclin, EGF-like, laminin-type EGF-like and link domain-containing scavengerreceptor 1
• FEEL1
• FEEL-1
• FEEL-1MS-1 antigen
• FELE-1
• FEX1
• KIAA0246common lymphatic endothelial and vascular endothelial receptor-1
• MS-1 Antigen
• STAB1
• STAB-1
• stabilin 1
• Stabilin1
• Stabilin-1

Background

Stabilin-1, also known as FEEL-1 (fasciclin, EGF-like, laminin type EGF-like, and link domain containing scavenger receptor 1) and CLEVER-1 (common lymphatic endothelial and vascular endothelial receptor 1), was originally identified as the MS-1 antigen. It is a type I transmembrane multi-domain protein with a large extracellular domain that contains 7 fasciclin, multiple EGF-like and laminin type EGF-like, and a link domain. The short cytoplasmic tail interacts with CGAs which are cargo adaptors that link cargo into the clathrin-coated pits. Stabilin-1 is expressed by tissue macrophages and sinusoidal endothelial cells, which are scavenging cells. Stabilin-1 is involved in intracellular trafficking pathways and binds multiple ligands including acetylated LDL, SPARC, AGE, and SI-CLP. By alternative splicing, a soluble Stabilin-1 containing a 58 residue substitution between amino acid residues 746 and 803, followed by truncation, has been identified. Within the region used as immunogen, human Stabilin-1 shares 83% and 84% amino acid sequence homology with mouse and canine Stabilin-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Stabilin-1 Antibody (AF3825)(1)

Publication using AF3825

• Bo Wang, Hao Huang, Meihua Yang, Wenjuan Yang, Zhuowei Liu, Weibin Hou et al. Microlocalization and clinical significance of stabilin-1+ macrophages in treatment-naïve patients with urothelial carcinoma of the bladder World Journal of Urology 2020-03-01 [PMID: 31302753]

Bioinformatics

• Gene Symbol: STAB1
• Uniprot:
  • Q9NY15()