Sheep IgG Isotype Control [Unconjugated]

Effects of SDF-1-CXCR4/CXCR7 pathway on MSC chemotaxis in vitro.(A) The chemotaxis in response to SDF-1 alpha (10 ng/ml for 12 h) was performed in the NP-MSCs and HP-MSCs treated with a neutralizing anti-CXCR4 antibody, an anti-CXCR7 antibody, and the respective isotype-matched control antibodies. *P<0.05, vs NP-MSCs; †P<0.05, vs the respective isotype-matched control antibodies. (B) NP-MSCs were transiently overexpressed with CXCR4 using pORF9-mCXCR4 vector or with CXCR7 using pORF9-mCXCR7 vector (n = 6). A negative control empty (pORF9-MCS) vector was used. (C) The transfected cells were subjected to chemotaxis in response to the indicated concentrations of SDF-1 alpha for 12 h. *P<0.05, vs the empty vector. (D and E) Western blot analysis (D) and FCM (E) were performed to determine the intracellular and extracellular expression of both CXCR4 and CXCR7 in the cells treated with or without SDF-1 alpha (50 ng/ml for 60 min). (F) The chemotaxis in response to SDF-1 alpha was performed in the cells treated with or without SDF-1 alpha . Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/22511954), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Sh
• Applications: Ctrl
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated

Sheep IgG Isotype Control [Unconjugated] Summary

• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IGHG1

Applications/Dilutions

• Dilutions:
  • Control

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C.
• Preservative: 0.05% Sodium Azide
• Reconstitution Instructions: Reconstitution default not known

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sheep IgG Isotype Control [Unconjugated]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (5-001-A)(42)

Publications using 5-001-A

• Zhao, X;Li, Y;Zhang, S;Sudwarts, A;Zhang, H;Kozlova, A;Moulton, MJ;Goodman, LD;Pang, ZP;Sanders, AR;Bellen, HJ;Thinakaran, G;Duan, J; Alzheimer's disease protective allele of Clusterin modulates neuronal excitability through lipid-droplet-mediated neuron-glia communication medRxiv : the preprint server for health sciences 2024-08-15 [PMID: 39185522] (Chromatin Immunoprecipitation (Negative), Human, Mouse)
• DE Fournier, MA Veras, CR Brooks, D Quinonez, M Millecamps, LS Stone, CA Séguin Stiffness and axial pain are associated with the progression of calcification in a mouse model of diffuse idiopathic skeletal hyperostosis Arthritis Research & Therapy, 2023-04-29;25(1):72. 4/29/2023 [PMID: 37120576]
• VA Stark, COB Facey, L Opdenaker, JZ Fields, BM Boman Differential miRNA Expression Contributes to Emergence of Multiple Cancer Stem Cell Subpopulations in Human Colorectal Cancer bioRxiv : the preprint server for biology, 2023-02-06;0(0):. 2023-02-06 [PMID: 36798319] (Flow Cytometry, Human)
• J Van Coilli, T Pongracz, J Rahmöller, HJ Chen, CE Geyer, LA van Vught, JS Buhre, T Šušti?, TLJ van Osch, M Steenhuis, W Hoepel, W Wang, AS Lixenfeld, J Nouta, S Keijzer, F Linty, R Visser, MD Larsen, EL Martin, I Künsting, S Lehrian, V von Kopylo, C Kern, HB Lunding, M de Winther, N van Mourik, T Rispens, T Graf, MA Slim, RP Minnaar, MK Bomers, JJ Sikkens, APJ Vlaar, CE van der Sc, J den Dunnen, M Wuhrer, M Ehlers, G Vidarsson, Fatebenefr, UMC COVID- The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not in antigen-experienced vaccinees EBioMedicine, 2022-12-16;87(0):104408. 2022-12-16 [PMID: 36529104] (Isotype Control, Human)
• Andrew Bond, Vito Bruno, Jason Johnson, Sarah George, Raimondo Ascione Development and Preliminary Testing of Porcine Blood-Derived Endothelial-like Cells for Vascular Tissue Engineering Applications: Protocol Optimisation and Seeding of Decellularised Human Saphenous Veins International Journal of Molecular Sciences 2022-06-14 [PMID: 35743073]
• Marco Gargaro, Giulia Scalisi, Giorgia Manni, Carlos G. Briseño, Prachi Bagadia, Vivek Durai et al. Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication Immunity 2022-06-14 [PMID: 35704993]
• K Matsumoto, V Kumar, S Varshney, AV Nairn, A Ito, F Pennarubia, KW Moremen, P Stanley, RS Haltiwange Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells The Journal of Biological Chemistry, 2022-05-25;0(0):102064. 2022-05-25 [PMID: 35623385] (Control, Human)
• D Ben Amar, K Thoinet, B Villalard, O Imbaud, C Costechare, L Jarrosson, F Reynaud, J Novion Duc, Y Couté, JF Brunet, V Combaret, N Corradini, C Delloye-Bo, V Castellani Environmental cues from neural crest derivatives act as metastatic triggers in an embryonic neuroblastoma model Nature Communications, 2022-05-10;13(1):2549. 2022-05-10 [PMID: 35538114] (Neutralization Control, Human)
• M Kschonsak, MC Johnson, R Schelling, EM Green, L Rougé, H Ho, N Patel, C Kilic, E Kraft, CP Arthur, AL Rohou, L Comps-Agra, N Martinez-M, L Perez, J Payandeh, C Ciferri Structural basis for HCMV Pentamer receptor recognition and antibody neutralization Science Advances, 2022-03-11;8(10):eabm2536. 2022-03-11 [PMID: 35275719] (Flow Cytometry Control, Human)
• R Gupta, P Gupta, Z Wang, A Seth, J Morrissey, I George, S Gandra, G Storch, B Parikh, G Genin, S Singamanen Plasmon-enhanced Quantitative Lateral Flow Assay for Femtomolar Detection of SARS-CoV-2 Antibodies and Antigens Research square, 2022-02-21;0(0):. 2022-02-21 [PMID: 35194598] (Bioassay, Human)
• Marlene Louise Quaade, Pratibha Dhumale, Simon Gabriel Comerma Steffensen, Hans Christian Beck, Eva Bang Harvald, Charlotte Harken Jensen et al. Adipose-Derived Stem Cells from Type 2 Diabetic Rats Retain Positive Effects in a Rat Model of Erectile Dysfunction International Journal of Molecular Sciences 2022-02-01 [PMID: 35163613]
• Y Kawana, H Suga, H Kamijo, T Miyagaki, M Sugaya, S Sato Roles of OX40 and OX40 Ligand in Mycosis Fungoides and S�zary Syndrome International Journal of Molecular Sciences, 2021-11-22;22(22):. 2021-11-22 [PMID: 34830466] (Neutralization Control, Mouse)
• John R. Buzzo, Aishwarya Devaraj, Erin S. Gloag, Joseph A. Jurcisek, Frank Robledo-Avila, Theresa Kesler et al. Z-form extracellular DNA is a structural component of the bacterial biofilm matrix Cell 2021-11-01 [PMID: 34735796]
• D Ali, D Alhattab, H Jafar, M Alzubide, N Sharar, S Bdour, A Awidi Differential Marker Expression between Keratinocyte Stem Cells and Their Progeny Generated from a Single Colony International Journal of Molecular Sciences, 2021-10-06;22(19):. 2021-10-06 [PMID: 34639148] (Flow Cytometry, Human)
• M Severa, RA Diotti, MP Etna, F Rizzo, S Fiore, D Ricci, M Iannetta, A Sinigaglia, A Lodi, N Mancini, E Criscuolo, M Clementi, M Andreoni, S Balducci, L Barzon, P Stefanelli, N Clementi, EM Coccia Differential plasmacytoid dendritic cell phenotype and type I Interferon response in asymptomatic and severe COVID-19 infection PloS Pathogens, 2021-09-02;17(9):e1009878. 2021-09-02 [PMID: 34473805] (Neutralization Control, Human)
• Ross A. Robinson, Samuel C. Griffiths, Lieke L. van de Haar, Tomas Malinauskas, Eljo Y. van Battum, Pavol Zelina et al. Simultaneous binding of Guidance Cues NET1 and RGM blocks extracellular NEO1 signaling Cell 2021-04-15 [PMID: 33740419]
• X Zhuang, F Maione, J Robinson, M Bentley, B Kaul, K Whitworth, N Jumbu, E Jinks, J Bystrom, P Gabriele, E Garibaldi, E Delmastro, Z Nagy, D Gilham, E Giraudo, R Bicknell, SP Lee CAR T cells targeting tumor endothelial marker CLEC14A inhibit tumor growth JCI Insight, 2020-10-02;5(19):. 2020-10-02 [PMID: 33004686] (Control, Mouse)
• C Cho, R Mukherjee, AR Peck, Y Sun, N McBrearty, KV Katlinski, J Gui, PK Govindaraj, E Puré, H Rui, SY Fuchs Cancer-associated fibroblasts downregulate type I interferon receptor to stimulate intratumoral stromagenesis Oncogene, 2020-08-17;0(0):. 2020-08-17 [PMID: 32807917] (ICC, IHC, Human)
• CJ Mietus, TJ Lackner, PS Karvelis, GT Willcockso, CM Shields, NG Lambert, P Koutakis, MA Fuglestad, H Hernandez, GR Haynatzki, JKS Kim, HK DeSpiegela, II Pipinos, GP Casale Abnormal Microvascular Architecture, Fibrosis, and Pericyte Characteristics in the Calf Muscle of Peripheral Artery Disease Patients with Claudication and Critical Limb Ischemia J Clin Med, 2020-08-08;9(8):. 2020-08-08 [PMID: 32784470] (ICC, IHC, Bovine, Human)
• J Robinson, K Whitworth, E Jinks, Z Nagy, R Bicknell, SP Lee An evaluation of the tumour endothelial marker CLEC14A as a therapeutic target in solid tumours J Pathol Clin Res, 2020-07-21;0(0):. 2020-07-21 [PMID: 32696621] (IHC, Human)
• CM Rival, W Xu, LS Shankman, S Morioka, S Arandjelov, CS Lee, KM Wheeler, RP Smith, LB Haney, BE Isakson, S Purcell, JJ Lysiak, KS Ravichandr Phosphatidylserine on viable sperm and phagocytic machinery in oocytes regulate mammalian fertilization Nat Commun, 2019-10-01;10(1):4456. 2019-10-01 [PMID: 31575859] (Bioassay, Mouse)
• T Kuroda, S Yasuda, S Tachi, S Matsuyama, S Kusakawa, K Tano, T Miura, A Matsuyama, Y Sato SALL3 expression balance underlies lineage biases in human induced pluripotent stem cell differentiation Nat Commun, 2019-05-15;10(1):2175. 2019-05-15 [PMID: 31092818] (Immunoprecipitation, Human)
• T Kato, S Pothula, RJ Liu, CH Duman, R Terwillige, GP Vlasuk, E Saiah, S Hahm, RS Duman Sestrin modulator NV-5138 produces rapid antidepressant effects via direct mTORC1 activation J. Clin. Invest., 2019-04-16;130(0):. 2019-04-16 [PMID: 30990795] (In Vivo, Rat)
• N Ding, Z Zou, H Sha, S Su, H Qian, F Meng, F Chen, S Du, S Zhou, H Chen, L Zhang, J Yang, J Wei, B Liu iRGD synergizes with PD-1 knockout immunotherapy by enhancing lymphocyte infiltration in gastric cancer Nat Commun, 2019-03-22;10(1):1336. 2019-03-22 [PMID: 30902997] (Neutralization, Human)
• JH Wang, ST Huang, L Zhang, ZG Liu, RX Liang, SW Jiang, YN Jiang, XJ Yu, YC Jiang, XZ Li, PF Zhang, ZS Wen, M Zheng Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma Cancer Med, 2019-02-11;0(0):. 2019-02-11 [PMID: 30741466] (IHC, Human)
• Martinez-Martin N, Marcandalli J, Huang C, Arthur C, Perotti M, Foglierini M, Ho H, Dosey A, Shriver S, Payandeh J, Leitner A, Lanzavecchia A, Perez L, Ciferri C An Unbiased Screen for Human Cytomegalovirus Identifies Neuropilin-2 as a Central Viral Receptor. Cell, 2018-07-26;174(5):1158-1171.e19. 2018-07-26 [PMID: 30057110] (Flow Cytometry, Human)
• Chisato Tomida, Naoko Yamagishi, Hikaru Nagano, Takayuki Uchida, Ayako Ohno, Katsuya Hirasaka et al. VEGF pathway-targeting drugs induce evasive adaptation by activation of neuropilin-1/cMet in colon cancer cells International Journal of Oncology 2018-02-28 [PMID: 29532881]
• WJ Zacharias, DB Frank, JA Zepp, MP Morley, FA Alkhaleel, J Kong, S Zhou, E Cantu, EE Morrisey Regeneration of the lung alveolus by an evolutionarily conserved epithelial progenitor Nature, 2018-02-28;555(7695):251-255. 2018-02-28 [PMID: 29489752] (Flow Cytometry, Mouse)
• A Lo, CP Li, EL Buza, R Blomberg, P Govindaraj, D Avery, J Monslow, M Hsiao, E Puré Fibroblast activation protein augments progression and metastasis of pancreatic ductal adenocarcinoma JCI Insight, 2017-10-05;2(19):. 2017-10-05 [PMID: 28978805] (IHC-P, Human)
• The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function PLoS ONE, 2016-08-16;11(8):e0161235. 2016-08-16 [PMID: 27529176] (IHC-P, Human)
• T Sadaoka, DP Depledge, L Rajbhandar, A Venkatesan, J Breuer, JI Cohen In vitro system using human neurons demonstrates that varicella-zoster vaccine virus is impaired for reactivation, but not latency Proc Natl Acad Sci USA, 2016-04-12;0(0):. 2016-04-12 [PMID: 27078099] (Functional Assay - Control, Human)
• Prolyl-isomerase Pin1 controls Notch3 protein expression and regulates T-ALL progression Oncogene, 2016-02-15;35(36):4741-51. 2016-02-15 [PMID: 26876201] (Neutralization, Human)
• Helen Schneck, Berthold Gierke, Frauke Uppenkamp, Bianca Behrens, Dieter Niederacher, Nikolas H. Stoecklein et al. EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer PLOS ONE 2015-12-22 [PMID: 26695635]
• Akihiko Murata, Miya Yoshino, Mari Hikosaka, Kazuki Okuyama, Lan Zhou, Seiji Sakano et al. An Evolutionary-Conserved Function of Mammalian Notch Family Members as Cell Adhesion Molecules PLoS ONE 2014-09-25 [PMID: 25255288]
• Grassot V, Da Silva A, Saliba J, Maftah A, Dupuy F, Petit J Highlights of glycosylation and adhesion related genes involved in myogenesis. BMC Genomics, 2014-07-22;15(0):621. 2014-07-22 [PMID: 25051993] (Neutralization, Mouse)
• Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin B, Zarbock A, Gerke V, Rescher U Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63. Nat Commun, 2014-04-28;5(0):3738. 2014-04-28 [PMID: 24769558] (Flow Cytometry, Human)
• Ishikawa T, Wondimu Z, Oikawa Y, Ingerpuu S, Virtanen I, Patarroyo M Monoclonal antibodies to human laminin alpha4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of alpha6beta1 integrin and MCAM to alpha4-laminins. Matrix Biol, 2014-03-27;36(0):5-14. 2014-03-27 [PMID: 24681327] (Neutralization, Human)
• Kevin Wilhelmsen, Katherine Farrar, Judith Hellman Quantitative <em>In vitro</em> Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions Journal of Visualized Experiments 2013-08-23 [PMID: 23995778] (Neutralization Control, Human)
• Stanietsky N, Rovis T, Glasner A, Seidel E, Tsukerman P, Yamin R, Enk J, Jonjic S, Mandelboim O Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR. Eur J Immunol, 2013-07-04;43(8):2138-50. 2013-07-04 [PMID: 23677581] (Blocking, Human)
• Dianne M. A. van den Heuvel, Anita J. C. G. M. Hellemons, R. Jeroen Pasterkamp Spatiotemporal Expression of Repulsive Guidance Molecules (RGMs) and Their Receptor Neogenin in the Mouse Brain PLoS ONE 2013-02-14 [PMID: 23457482]
• Dunne E, Spring CM, Reheman A, Jin W, Berndt MC, Newman DK, Newman PJ, Ni H, Kenny D Cadherin 6 has a functional role in platelet aggregation and thrombus formation. Arterioscler. Thromb. Vasc. Biol., 2012-04-26;32(7):1724-31. 2012-04-26 [PMID: 22539596] (Control, Human)
• Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury. PLoS ONE, 2012-04-12;7(4):e34608. 2012-04-12 [PMID: 22511954] (Control, Mouse)

Review for IgG Isotype Control (5-001-A) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 02/14/2018

Summary

• Lot: WAX0417011

Comments

• Comments: For surface staining of PANC1 cells as Isotype control along with Anti sheep Alexa 647 secondary Antibody

Bioinformatics

• Gene Symbol: IGHG1