LYVE-1 Antibody [Unconjugated]

Images

 
LYVE‑1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained ...read more
Western blot shows lysates of mouse liver tissue and bEnd.3 mouse brain endothelial cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LYVE‑1 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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LYVE-1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Specificity
Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human LYVE-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LYVE1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF2125 in the following applications:

Publications
Read Publications using
AF2125 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LYVE-1 Antibody [Unconjugated]

  • cell surface retention sequence binding protein-1
  • Cell surface retention sequence-binding protein 1
  • CRSBP1
  • CRSBP-1
  • extracellular link domain containing 1
  • extracellular link domain-containing 1
  • Extracellular link domain-containing protein 1
  • HAR
  • Hyaluronic acid receptor
  • lymphatic vessel endothelial hyaluronan receptor 1
  • lymphatic vessel endothelial hyaluronic acid receptor 1
  • LYVE1
  • LYVE-1
  • LYVE-1XLKD1
  • XLKD1

Background

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively. LYVE-1 shares 41% homology with the other known HA receptor, CD44. The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican. Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels. In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells. LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes. Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LYVE-1 Antibody (AF2125)(30)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 11 applications: Cell selection, ICC, IHC, IHC Fresh, IHC Frozen, IHC Not Specified, IHC Paraffin-embedded, IHC-Frozen, IHC-OCT embedded, IHC-P, IP.


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Cell selection
(1)
ICC
(3)
IHC
(10)
IHC Fresh
(2)
IHC Frozen
(2)
IHC Not Specified
(2)
IHC Paraffin-embedded
(3)
IHC-Frozen
(1)
IHC-OCT embedded
(1)
IHC-P
(2)
IP
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(27)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using AF2125 Applications Species
CZ Li, XJ Jiang, B Lin, HJ Hong, SY Zhu, L Jiang, XQ Wang, NH Tang, FF She, YL Chen RIP1 regulates TNF-?-mediated lymphangiogenesis and lymphatic metastasis in gallbladder cancer by modulating the NF-?B-VEGF-C pathway Onco Targets Ther, 2018;11(0):2875-2890. 2018 [PMID: 29844685] (IHC,, Mouse) IHC Mouse
GD García Nor, CL Ly, DA Cuzzone, RP Kataru, GE Hespe, JS Torrisi, JJ Huang, JC Gardenier, IL Savetsky, MD Nitti, JZ Yu, S Rehal, BJ Mehrara CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema Nat Commun, 2018;9(1):1970. 2018 [PMID: 29773802] (IHC,, Mouse) IHC Mouse
D Hominick, A Silva, N Khurana, Y Liu, PC Dechow, JQ Feng, B Pytowski, JM Rutkowski, K Alitalo, MT Dellinger VEGF-C promotes the development of lymphatics in bone and bone loss Elife, 2018;7(0):. 2018 [PMID: 29620526] (IHC-P,, Mouse) IHC-P Mouse
BF Westendorp, NVJA Büller, ON Karpus, WA van Dop, J Koster, R Versteeg, PJ Koelink, CY Snel, S Meisner, JJTH Roelofs, A Uhmann, E Ver Loren, J Heijmans, H Hahn, V Muncan, ME Wildenberg, GR van den Br Indian Hedgehog Suppresses a Stromal Cell-Driven Intestinal Immune Response Cell Mol Gastroenterol Hepatol, 2018;5(1):67-82.e1. 2018 [PMID: 29276753] (IHC-P,, Mouse) IHC-P Mouse
M García-Cab, J Paupert, S Blacher, M Van de Vel, AR Quesada, MA Medina, A Noël Targeting VEGFR-3/-2 signaling pathways with AD0157: a potential strategy against tumor-associated lymphangiogenesis and lymphatic metastases J Hematol Oncol, 2017;10(1):122. 2017 [PMID: 28629427] (IHC,, Mouse) IHC Mouse
X Jiang, C Li, B Lin, H Hong, L Jiang, S Zhu, X Wang, N Tang, X Li, F She, Y Chen cIAP2 promotes gallbladder cancer invasion and lymphangiogenesis by activating the NF-?B pathway Cancer Sci., 2017;108(6):1144-1156. 2017 [PMID: 28295868] (IHC,, Mouse) IHC Mouse
Boksik Cha Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves Genes Dev, 2016;30(12):1454-69. 2016 [PMID: 27313318]
Proteolytic activation defines distinct lymphangiogenic mechanisms for VEGFC and VEGFD J Clin Invest, 2016;0(0):. 2016 [PMID: 27159393] (IHC Paraffin-embedded,, Mouse) IHC Paraffin-embedded Mouse
Thy1+IL-7+ lymphatic endothelial cells in iBALT provide a survival niche for memory T-helper cells in allergic airway inflammation Proc Natl Acad Sci USA, 2016;113(20):E2842-51. 2016 [PMID: 27140620] (IHC Not Specified,, Mouse) IHC Not Specified Mouse
CHD4-regulated plasmin activation impacts lymphovenous hemostasis and hepatic vascular integrity J Clin Invest, 2016;0(0):. 2016 [PMID: 27140400] (IP,, Mouse) IP Mouse
Show All 30 Publications.

Review for LYVE-1 Antibody (AF2125) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF2125:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
Flow Mouse 01/06/2015
View

Summary

ApplicationFlow Cytometry
Sample TestedSee PMID 22287283
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22287283

Details

Detection NotesSee PMID 22287283

Comments

CommentsPublished: See PMID 22287283

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FAQs for LYVE-1 Antibody (AF2125) (0)

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Blogs on LYVE-1.

Meningeal lymphatics: recent discovery defying the concept of central nervous system 'immune privilege'
By Jennifer Sokolowski, MD, PhD. Identification and characterization of meningeal lymphaticsThe recent discovery of a lymphatic system in the meninges surrounding the brain and spinal cord has spurred a surge of int...  Read full blog post.

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Recent Reviews

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Anonymous
01/06/2015
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol LYVE1
Entrez
Uniprot