SREC-I/SCARF1 Antibody

HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human SREC‑I/SR‑F1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF2409, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SREC-I/SCARF1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SREC‑I/SCARF1
  Ser20-Thr421
  Accession # Q14162
• Specificity: Detects SREC‑I/SCARF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SREC-II is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SCARF1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SREC-I/SCARF1 Antibody

• Acetyl LDL receptor
• KIAA0149endothelial cells scavenger receptor
• MGC47738
• SCARF1
• scavenger receptor class F, member 1
• Scavenger receptor expressed by endothelial cells 1
• SREC1
• SRECI
• SREC-I
• SRECscavenger receptor expressed by endothelial cells
• SR-F1

Background

The scavenger receptor (SR) family comprises a group of functionally defined membrane receptors that share a common ability to bind and internalize modified forms of low density lipoproteins (LDL) such as acetylated LDL (AcLDL) and oxidized LDL (OxLDL) (1‑3). Family members are classified alphabetically. They play important roles in lipid metabolism, in host defence and in the regulation of acquired immunity (2, 4). Scavenger receptor expressed by endothelial cells-I (SREC-I) and SREC-II are two proteins that belong to the F type scavenger receptor group (SR-F1 and SR-F2). The full length cDNA of human SREC-I encodes an 830 amino acid (aa) type I transmembrane protein which contains a 19 aa signal peptide, a 402 aa extracellular region, a 21 aa transmembrane segment, and a 388 aa long cytoplasmic domain. The extracellular region contains ten EGF-like repeats (five of which fit the exact consensus sequence for an EGF-like domain) while the cytoplasmic domain is rich in serine and proline in the N-terminal half, and glycine in the C-terminal segment (5, 6). In addition to the full length form, four SREC-I isoforms exist. Two show insertions of a stop codon in EGF-like domain #8, resulting in mature soluble forms of 323 aa and 318 aa, respectively. A third isoform deletes part of domain #8 plus domains #9 and #10; it continues in-frame to generate a mature transmembrane protein of 725 aa. The last isoform shows only cytoplasmic splicing, with 72 aa substituted for the last 332 aa of the full length form. All three transmembrane forms bind acetylated LDL (6). Native SREC-I is approximately 150 kDa in size, and expressed by endothelial cells, macrophages and fetal neurons (7, 8). In the extracellular region human SREC-I is 76% aa identical to mouse SREC-I. The extracellular regions of human SREC-I and II are 53% aa identical.

1. Horiuchi, S. et al. (2003) Amino Acids 25:283.
2. Greaves, D.R. and S. Gordon (2005) J. Lipid Res. 46:11.
3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
4. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
5. Adachi, H. et al. (1997) J. Biol. Chem. 272:31217.
6. Adachi, H. and M. Tsujimoto (2002) J. Biol. Chem. 277:24014.
7. Shibata, M. et al. (2004) J. Biol. Chem. 279:40084.
8. Tanura, Y. et al. (2004) J. Biol. Chem. 279:30938.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SREC-I/SCARF1 Antibody (AF2409)(4)

Publications using AF2409

• Baur S, Rautenberg M, Faulstich M, Grau T, Severin Y, Unger C, Hoffmann W, Rudel T, Autenrieth I, Weidenmaier C A nasal epithelial receptor for Staphylococcus aureus WTA governs adhesion to epithelial cells and modulates nasal colonization. PLoS Pathog, 2014;10(5):e1004089. 2014 [PMID: 24788600] (ICC, Cotton Rat)
• Dieudonne A, Torres D, Blanchard S, Taront S, Jeannin P, Delneste Y, Pichavant M, Trottein F, Gosset P Scavenger receptors in human airway epithelial cells: role in response to double-stranded RNA. PLoS ONE, 2012;7(8):e41952. 2012 [PMID: 22879901] (Flow Cytometry, Human)
• Narazaki M, Segarra M, Hou X, Tanaka T, Li X, Tosato G Oligo-guanosine nucleotide induces neuropilin-1 internalization in endothelial cells and inhibits angiogenesis. Blood, 2010;116(16):3099-107. 2010 [PMID: 20606164] (Flow Cytometry, Human)
• Narazaki M, Segarra M, Tosato G Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 2008;111(8):4126-36. 2008 [PMID: 18272814] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SCARF1
• Uniprot:
  • Q14162()