LIMPII/SR-B2 Antibody

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A) and aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated by dashed yellow line). Rhodamine phalloidin (red), which labels the cortical actin cytoskeleton, was used to show cell borders. Umbrella cells show an apparent, age-related increase in LIMP2 staining (white arrows). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY licence.

Western Blot: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 (5), HEPG2 human liver lysate (6).

Simple Western: LIMPII/SR-B2 Antibody [NB400-129] - CHAPS-solublized SVG-A (human astrocyte cells) and B103 (rat neuroblastoma cells) cell lyates, at the indicated concentrations, were probed with a 1:25 dilution of antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A) and aged (C) urothelium incubated with Lysotracker Red (red) and co-stained with antibodies to LIMP2 (green structures) and phalloidin (blue), which labels the cortical actin cytoskeleton. Large, Lysotracker Red-positive endolysosomes are marked with white arrows. Underlying intermediate (IC) and basal cells (BC) show non-specific cytoplasmic Lysotracker Red staining that did not appear in umbrella cells (UC). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0198817), licensed under a CC-BY licence.

Immunocytochemistry/Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMPII/lpg85 (Green) in Hela cells using NB400-129. Nuclei (Blue) are counterstained using Hoechst 33258.

Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129] - Substitution V135A enhances PSGL1 usage by mouse-adapted EV-A71. LIMPII/SR-B2 (SCARB2) and PSGL1 in various organs tissues assessed by means of immunohistochemistry. SCARB2 protein expression in CNS, intestine, pancreas, liver and lung. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31339457/) licensed under a CC-BY licence.

Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129] - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LIMPII/SR-B2 Antibody Summary

• Immunogen: A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]
• Localization: Lysosome membrane; Multi-pass membrane protein.
• Marker: Lysosome Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCARB2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
  • Simple Western
  • Western Blot 1:1000
• Application Notes: This LIMPII/lpg85 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot, where a band is observed at ~85 kDa. Use in IHC-P was reported in scientific literature (PMID: 23863627). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NIH 3T3 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for LIMPII/SR-B2 Antibody

• AMRF
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
• CD36 antigen
• CD36L2
• CD36L2LIMP II
• HLGP85
• LGP85
• LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
• LIMPII
• LIMPIICD36 antigen-like 2
• LPG85
• lysosome membrane protein 2
• Lysosome membrane protein II
• SCARB2
• Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
• scavenger receptor class B, member 2
• SRB2
• SR-B2
• SR-BII

Background

LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIMPII/SR-B2 Antibody (NB400-129)(21)

Publications using NB400-129

• Gletten RB, Cantrell LS, Bhattacharya S, Schey KL Lens Aquaporin-5 Inserts Into Bovine Fiber Cell Plasma Membranes Via Unconventional Protein Secretion Investigative ophthalmology & visual science 2022-07-08 [PMID: 35816045]
• Lee JH, Yang DS, Goulbourne CN et al. Faulty autolysosome acidification in Alzheimer's disease mouse models induces autophagic build-up of A beta in neurons, yielding senile plaques Nature neuroscience 2022-06-01 [PMID: 35654956] (ICC/IF, Mouse)
• Bertolin J, SAnchez V, Ribera A et al. Treatment of skeletal and non-skeletal alterations of Mucopolysaccharidosis type IVA by AAV-mediated gene therapy Nature communications 2021-09-09 [PMID: 34504088] (IHC-P, Rat)
• van der Lienden M, Aten J, Marques A et al. GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice International Journal of Molecular Sciences 2021-03-03 [PMID: 33802460] (IHC-P, Mouse)
• Lee JH, Wolfe DM, Darji S et al. Beta 2-adrenergic agonists rescue lysosome acidification and function in PSEN1 deficiency by reversing defective ER to lysosome delivery of ClC-8 J. Mol. Biol. 2020-02-24 [PMID: 32105735]
• Wiersma VI, van Ziel AM, Vazquez-Sanchez S, et al. Granulovacuolar degeneration bodies are neuron-selective lysosomal structures induced by intracellular tau pathology Acta Neuropathol. 2019-12-01 [PMID: 31456031] (IHC, Mouse)
• Malla SR, Krueger B, Wartmann T et al. Early trypsin activation develops independently of autophagy in caerulein-induced pancreatitis in mice Cell. Mol. Life Sci. 2019-07-30 [PMID: 31363815] (Mouse)
• Sun L, Tijsma A, Mirabelli C et al. Intra-host emergence of an enterovirus A71 variant with enhanced PSGL1 usage and neurovirulence Emerg Microbes Infect 2019-07-25 [PMID: 31339457] (IHC-P, Mouse)
• Wilden AR, Molina JA, Feuerborn M et al. Glutamine-dependent lysosome homeostatic changes induced by starvation and lysosome inhibition. Biochim. Biophys. Acta 2018-09-01 [PMID: 29966622] (ICC/IF, Human)
• Truschel ST, Clayton DR, Beckel JM et al. Age-related endolysosome dysfunction in the rat urothelium PLoS ONE 2018-06-08 [PMID: 29883476] (ICC/IF, Rat)
• Kondo H, Maksimova N, Otomo T et al. Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms. Hum. Mol. Genet. 2016-12-23 [PMID: 28013294] (Human)
• Mareninova OA, Sendler M, Malla SR et al. Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis. Cell Mol Gastroenterol Hepatol 2015-11-01 [PMID: 26693174] (Mouse)
• Gurda BL, De Guilhem De Lataillade A, Bell P et al. Evaluation of AAV-mediated Gene Therapy for Central Nervous System Disease in Canine Mucopolysaccharidosis VII. Mol Ther 2016-02-01 [PMID: 26447927]
• Ikari A, Tonegawa C, Sanada A et al. Tight Junctional Localization of Claudin-16 is Regulated by Syntaxin 8 in Renal Tubular Epithelial Cells. J. Biol. Chem. 2014-03-21 [PMID: 24659781] (ICC/IF, Canine)
• Fruhwurth S, Pavelka M, Bittman R et al. High-density lipoprotein endocytosis in endothelial cells. World J Biol Chem 2013-11-26 [PMID: 24340136] (ICC/IF, Human)
• Gaspar P, Kallemeijn WW, Strijland A et al. Action myoclonus-renal failure syndrome: diagnostic applications of activity-based probes and lipid analysis. J. Lipid Res. 2014-01-01 [PMID: 24212238] (WB, Human)
• Haurigot V, Marco S, Ribera A et al. Whole body correction of mucopolysaccharidosis IIIA by intracerebrospinal fluid gene therapy. J Clin Invest 2013-07-01 [PMID: 23863627] (IHC-P, Mouse)
• Lee D, Desmond MJ, fraser SA et al. Expression of the Transmembrane Lysosomal Protein SCARB2/Limp-2 in Renin Secretory Granules Controls Renin Release. Nephron Exp Nephrol 2013-04-26 [PMID: 23635510]
• Gleich K, Desmond MJ, Lee D et al. Abnormal Processing of Autophagosomes in Transformed B Lymphocytes from SCARB2-Deficient Subjects. Biores Open Access 2013-02-01 [PMID: 23515316] (WB, Human)
• Balreira A, Gaspar P, Caiola D et al. A nonsense mutation in the LIMP-2 gene associated with progressive myoclonic epilepsy and nephrotic syndrome. Hum Mol Genet;17(14):2238-43. 2008-07-15 [PMID: 18424452] (WB, Human)
• van der Lienden MJC, Aten J, Boot R, van Eijk M Glucocerebrosidase in cultured cells: extreme sensitivity for medium conditions Thesis 2022-01-01 (ICC/IF)

Review for LIMPII/SR-B2 Antibody (NB400-129) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human and Rat.

reviewed by: Julian Wong Simple Western Human and Rat 09/03/2018

Summary

• Application: Simple Western
• Sample Tested: B103 lysate,SVG-A lysate
• Species: Human and Rat
• Lot: B-6

FAQs for LIMPII/SR-B2 Antibody (NB400-129). (Showing 1 - 1 of 1 FAQs).

1. Can you please inform me about the peptide used as immunogen? It is referred that is the a C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence, but the amino acid sequence used is not indicated.
   • NB400-129 was made to a sequence between amino acids 425-480.

Bioinformatics

• Gene Symbol: SCARB2
• Entrez:
  • 950(Human)
  • 12492(Mouse)