LIMPII/SR-B2 Antibody

Images

 
Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Comparison of LIMP2-positive organelles in young (A) and aged (C) rat urothelium. LIMP2-positive structures (green) shown in umbrella cells (delineated ...read more
Western Blot: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMP II (~85 kDa) in Lane 1: Rat cardiac fibroblasts (RCF), 3T3 mouse fibroblasts (2), canine lung lysate (3), RCF treated with angiotensin II (4), and H2O2 ...read more
Simple Western: LIMPII/SR-B2 Antibody [NB400-129] - CHAPS-solublized SVG-A (human astrocyte cells) and B103 (rat neuroblastoma cells) cell lyates, at the indicated concentrations, were probed with a 1:25 dilution of ...read more
Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody [NB400-129] - Lysotracker Red accumulates in endolysosomes.Immunofluorescence of young (A) and aged (C) urothelium incubated with Lysotracker Red (red) and co-stained ...read more
Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody [NB400-129] - Detection of LIMPII/lpg85 (Green) in Hela cells using NB400-129. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: LIMPII/SR-B2 Antibody [NB400-129] - Staining of LIMP II in rat infarctzone tissue with a DAB detection system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

LIMPII/SR-B2 Antibody Summary

Immunogen
A C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence. [UniProt# O35114]
Localization
Lysosome membrane; Multi-pass membrane protein.
Marker
Lysosome Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:100
Application Notes
This LIMPII/lpg85 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot, where a band is observed at ~85 kDa. Use in IHC-P was reported in scientific literature (PMID: 23863627). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB400-129 in the following applications:

Publications
Read Publications using
NB400-129 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for LIMPII/SR-B2 Antibody

  • AMRF
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
  • CD36 antigen
  • CD36L2
  • CD36L2LIMP II
  • HLGP85
  • LGP85
  • LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
  • LIMPII
  • LIMPIICD36 antigen-like 2
  • LPG85
  • lysosome membrane protein 2
  • Lysosome membrane protein II
  • SCARB2
  • Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
  • scavenger receptor class B, member 2
  • SRB2
  • SR-B2
  • SR-BII

Background

LIMP II (lysosome membrane protein 2; also called LGP85, SCARB2, CD36 antigen-like 2/CD36L2) is a member of the scavenger receptor class B member 2 family, ubiquitously expressed in higher organisms as a major lysosomal and endosomal membrane protein. LIMP II localize primarily to late endosomes/lysosomes as type III transmembrane protein, which traverses the membrane twice with the uncleaved signal peptide and a hydrophobic region near to the COOH-terminus, followed by twenty-amino acid long cytoplasmic tail of which a large portion located in lysosomal lumenal side possess 10-11 N-glycosylation sites. LIMP II acts as a lysosomal receptor for glucosylceramidase (GBA) targeting and it plays a key role in regulating the biogenesis of the endosomal/lysosomal compartments (ELCs). LIMP II serves as one of the major components of the molecular machinery required for intercompartmental membrane trafficking along the endocytic pathway rather than solely serving as a structural constituent of late endosomes and lysosomes. LIMP II deficiency has been found to result in the development of an obstruction of ureteric pelvic junction, deafness and peripheral neuropathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIMPII/SR-B2 Antibody (NB400-129)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(1)
IHC-P
(2)
WB
(3)
All Applications
Filter By Species
Human
(6)
Mouse
(5)
Rat
(1)
Canine
(1)
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB400-129 Applications Species
Lee JH, Wolfe DM, Darji S et al. Beta 2-adrenergic agonists rescue lysosome acidification and function in PSEN1 deficiency by reversing defective ER to lysosome delivery of ClC-7 J. Mol. Biol. Feb 24 2020 [PMID: 32105735]
Wiersma VI, van Ziel AM, Vazquez-Sanchez S, et al. Granulovacuolar degeneration bodies are neuron-selective lysosomal structures induced by intracellular tau pathology Acta Neuropathol. Dec 1 2019 [PMID: 31456031] (IHC, Mouse) IHC Mouse
Malla SR, Krueger B, Wartmann T et al. Early trypsin activation develops independently of autophagy in caerulein-induced pancreatitis in mice Cell. Mol. Life Sci. Jul 30 2019 [PMID: 31363815] (Mouse) Mouse
Sun L, Tijsma A, Mirabelli C et al. Intra-host emergence of an enterovirus A71 variant with enhanced PSGL1 usage and neurovirulence Emerg Microbes Infect Jul 25 2019 [PMID: 31339457] (IHC-P, Mouse) IHC-P Mouse
Wilden AR, Molina JA, Feuerborn M et al. Glutamine-dependent lysosome homeostatic changes induced by starvation and lysosome inhibition. Biochim. Biophys. Acta Sep 1 2018 [PMID: 29966622] (ICC/IF, Human) ICC/IF Human
Truschel ST, Clayton DR, Beckel JM et al. Age-related endolysosome dysfunction in the rat urothelium PLoS ONE Jun 8 2018 [PMID: 29883476] (ICC/IF, Rat) ICC/IF Rat
Kondo H, Maksimova N, Otomo T et al. Mutation in VPS33A affects metabolism of glycosaminoglycans: a new type of mucopolysaccharidosis with severe systemic symptoms. Hum. Mol. Genet. Dec 23 2016 [PMID: 28013294] (Human) Human
Mareninova OA, Sendler M, Malla SR et al. Lysosome associated membrane proteins maintain pancreatic acinar cell homeostasis: LAMP-2 deficient mice develop pancreatitis. Cell Mol Gastroenterol Hepatol 2015 Nov 1 [PMID: 26693174] (Mouse) Mouse
Gurda BL, De Guilhem De Lataillade A, Bell P et al. Evaluation of AAV-mediated Gene Therapy for Central Nervous System Disease in Canine Mucopolysaccharidosis VII. Mol Ther 2016 Feb [PMID: 26447927]
Ikari A, Tonegawa C, Sanada A et al. Tight Junctional Localization of Claudin-16 is Regulated by Syntaxin 8 in Renal Tubular Epithelial Cells. J. Biol. Chem. 3/21/2014 [PMID: 24659781] (ICC/IF, Canine) ICC/IF Canine
Show All 16 Publications.

Review for LIMPII/SR-B2 Antibody (NB400-129) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Rat.

Reviews using NB400-129:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Human and Rat
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western LIMPII/SR-B2 NB400-129
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4
reviewed by:
Anonymous
Simple Western Human and Rat 09/03/2018
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Summary

ApplicationSimple Western
Sample TestedSVG-A lysate,B103 lysate
SpeciesHuman and Rat
LotB-6

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LIMPII/SR-B2 Antibody (NB400-129). (Showing 1 - 1 of 1 FAQs).

  1. Can you please inform me about the peptide used as immunogen? It is referred that is the a C-terminal synthetic peptide made to the mouse LIMPII/lgp85 protein sequence, but the amino acid sequence used is not indicated.
    • NB400-129 was made to a sequence between amino acids 425-480.

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Secondary Antibodies

 

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
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Anonymous
09/03/2018
Application: Simple Western
Species: Human and Rat

Bioinformatics

Gene Symbol SCARB2
Entrez