SR-AI/MSR Antibody - BSA Free

Western Blot: SR-AI/MSR Antibody [NBP1-00092] - Detection of Macrophage Scavenger Receptor I (MSR1) protein in the lysate of human liver.

Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NBP1-00092] - The MSR antibody was tested in Raw cells at a 1:250 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.

Immunohistochemistry-Frozen: SR-AI/MSR Antibody - BSA Free [NBP1-00092] - Immune labeling of non-parenchymal liver cell (NPC) cultures for selected SRs and C-type lectins. NPCs from the 25-45% interface on the Percoll gradient were incubated for 1 h, then fixed 15 min in 4% paraformaldehyde, and double immune-labeled with antibodies to Fc-gamma-RIIb2 (SE-1; red fluorescence; left column), or CD68 (red fluorescence; right column), and to either stabilin-2 (STAB2; green), mannose receptor (MRC1; green), SR-A1 (green), or SR-B1 (green). Overlap of green and red fluorescence is seen as yellow staining in the overlay images. Cell nuclei were stained with DAPI (blue). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33246411/) licensed under a CC-BY license.

Flow Cytometry: SR-AI/MSR Antibody [NBP1-00092] - A surface stain was performed on Raw264.7 cells with SR-AI/MSR Antibody NBP1-00092R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

SR-AI/MSR was detected in immersion fixed SJCRH30 human Rhabdomyosarcoma cell line using Rabbit anti-SR-AI/MSR Antigen Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-00092AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

An intracellular stain was performed on SJCRH30 human Rhabdomyosarcoma cell line with Rabbit anti-SR-AI/MSR Affinity-purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-00092AF647, blue histogram) or matched control antibody (NBP2-24891AF647, orange histogram) at 5 µg/mL for 30 minutes at RT.

SR-AI/MSR was detected in immersion fixed SJCRH30 human Rhabdomyosarcoma cell line using Rabbit anti-SR-AI/MSR Antigen Affinity Purified Polyclonal Antibody conjugated to Biotin (Catalog # NBP1-00092B) at 5 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Eq, Pm
• Applications: WB, Flow, Func, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.00 mg/ml

SR-AI/MSR Antibody - BSA Free Summary

• Immunogen: Synthetic peptide made to an internal portion of human Macrophage Scavenger Receptor I (within residues 400-450). [Swiss-Prot: P21757]
• Localization: Membrane; Single-pass type II membrane protein.
• Predicted Species: Rat (92%), Canine (100%), Primate (93%), Equine (100%), Bovine (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2-5 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry reported in scientific literature (PMID 26358193)
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay reported in scientific literature (PMID 28338748)
  • Western Blot 0.5 ug/ml
• Application Notes: In Western blot, a band is seen ~50 kDa.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  SR-AI/MSR Overexpression Lysate

• Control Peptide:

  SR-AI/MSR Peptide (NBP1-00092PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.00 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SR-AI/MSR Antibody - BSA Free

• CD204 antigen
• CD204
• Macrophage acetylated LDL receptor I and II
• macrophage scavenger receptor 1
• macrophage scavenger receptor type III
• MSR1
• phSR1
• phSR2
• SCARA1
• SCARA1macrophage scavenger receptor types I and II
• Scavenger receptor class A member 1
• scavenger receptor class A, member 1
• SR-A
• SRAI
• SR-AI

Background

Macrophage Scavenger Receptor I (MSR1, also called CD204 or scavenger receptor A) belongs to scavenger receptor A family and represents phagocytic pattern-recognition receptors (PRRs) expressed predominantly on macrophages and dendritic cells. MSR1 is a single-pass type II membrane glycoprotein capable of binding to a wide range of polyanionic ligands (e.g., modified low density lipoproteins, pathogen-derived molecules), and is implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis, innate immune response as well as Alzheimer's disease. MSR1 mediates the endocytosis of a diverse group of macromolecules including modified LDLs, and scavenger receptors MSR1, CD36 and LOX-1 are three main receptors for oxidized LDL on macrophage membrane wherein MSR1/CD36 account for more than 9/10 of the lipid accumulation in macrophages exposed to oxidized LDL. Defects in MSR1 have also been linked to prostate cancer and Barrett esophagus (BE).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SR-AI/MSR Antibody (NBP1-00092)(8)

Publications using NBP1-00092

• Wang P, Li M, Gao T et al. Vascular Electrical Stimulation with Wireless, Battery-Free, and Fully Implantable Features Reduces Atherosclerotic Plaque Formation Through Sirt1-Mediated Autophagy Small (Weinheim an der Bergstrasse, Germany) 2023-06-02 [PMID: 37267941] (WB, Mouse)
• Ma C, Feng K, Yang X, et al. Targeting macrophage liver X receptor by hydrogel-encapsulated T0901317 reduces atherosclerosis without effect on hepatic lipogenesis British journal of pharmacology 2021-01-28 [PMID: 33506494]
• Bhandari, S, Li, R Et al. Transcriptome and proteome profiling reveal complementary scavenger and immune features of rat liver sinusoidal endothelial cells and liver macrophages. BMC Mol Cell Biol 2020-11-27 [PMID: 33246411] (WB, Mouse)
• Chen J, Koduri S, Dai S et al. Intra-hematomal White Matter Tracts Act As a Scaffold for Macrophage Infiltration After Intracerebral Hemorrhage Translational Stroke Research 2021-10-01 [PMID: 33094829] (Immunohistochemistry-Frozen, Immunocytochemistry/ Immunofluorescence, Mouse)
• Cao L, Sun PL, He Y et al. Immune stromal features in cervical squamous cell carcinoma are prognostic factors for distant metastasis: A retrospective study Pathol. Res. Pract. 2019-11-18 [PMID: 31776057] (IF/IHC, Human)
• Komai K, Ito M, Kanamori M et al. Role of scavenger receptors as damage-associated molecular pattern receptors in Toll-like receptor activation. International Immunology. 2017-02-24 [PMID: 28338748] (PLA, Mouse)
• Bartels ED, Christoffersen C, Lindholm MW, Nielsen LB. Altered Metabolism of LDL in the Arterial Wall Precedes Atherosclerosis Regression. Circ. Res. 2015-09-10 [PMID: 26358193] (IF/IHC, Mouse)
• Piccolo P, Vetrini F, Mithbaokar P et al. SR-A and SREC-I Are Kupffer and Endothelial Cell Receptors for Helper-dependent Adenoviral Vectors. Mol Ther 2013-01-29 [PMID: 23358188] (ICC/IF, IHC-Fr, Mouse)

Reviews for SR-AI/MSR Antibody (NBP1-00092) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Canine.

reviewed by: Verified Customer ICC Canine 06/13/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Dog blood cells on slide
• Species: Canine
• Lot: b-9

reviewed by: A G ICC Canine 03/24/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Peripheral blood leukocytes
• Species: Canine
• Lot: B9

Bioinformatics

• Gene Symbol: MSR1