SR-BI Antibody [DyLight 550]

Images

 
Flow Cytometry: SR-BI Antibody [DyLight 550] [NB400-104R] - SR-B1 staining of human adipose tissue macrophage populations compared to isotype control (orange). Image from verified customer review.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, Ha, Md, Pm, Rb, SyHaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

SR-BI Antibody [DyLight 550] Summary

Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB400-104R in the following applications:

Reactivity Notes

Mouse, rat, mink, hamster, primate, rabbit and the long form of human SR-BI. Reactivity with CHO cells reported in scientific literature (PMID: 12356718).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SR-BI Antibody [DyLight 550]

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-104R
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Publications for SR-BI Antibody (NB400-104R) (0)

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Review for SR-BI Antibody (NB400-104R) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB400-104R:
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Images Ratings Applications Species Date Details
Flow Cytometry SR-BI NB400-104R
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reviewed by:
Shawn Rose
Flow Human 04/28/2014
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Summary

ApplicationFlow Cytometry
Sample Testedhuman adipose tissue macrophages
SpeciesHuman

Blocking

Blocking Details10% bovine serum in PBS

Primary Anitbody

Dilution RatioSR-B1

Details

Detection NotesLSRII flow cytometer
Fixation Detailsfreshly isolated cells by collagenase digestion
Permeabilization Detailsnot applicable
Wash Descriptionsame as above

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-104R) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SR-BI Antibody (NB400-104R)

Discover related pathways, diseases and genes to SR-BI Antibody (NB400-104R). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for SR-BI Antibody (NB400-104R)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Recent Reviews

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1
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Shawn Rose
04/28/2014
Application: Flow
Species: Human

Bioinformatics

Gene Symbol SCARB1
Entrez