SR-BI Antibody [DyLight 550]


Flow Cytometry: SR-BI Antibody [DyLight 550] [NB400-104R] - SR-B1 staining of human adipose tissue macrophage populations compared to isotype control (orange). Image from verified customer review.

Product Details

Reactivity Hu, Mu, Rt, ChHa, SyHa, Ha, Md, Pm, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

SR-BI Antibody [DyLight 550] Summary

A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Cell Membrane
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C in the dark.
DyLight 550
DyLight 550 (A=562, E=576)
50mM Sodium Borate
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
This SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa
Reviewed Applications
Read 1 Review rated 5
NB400-104R in the following application:

Reactivity Notes

Mouse, rat, mink, hamster, primate, rabbit and the long form of human SR-BI. Reactivity with CHO cells reported in scientific literature (PMID: 12356718).

Alternate Names for SR-BI Antibody [DyLight 550]

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI


SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SR-BI Antibody (NB400-104R) (0)

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Reviews for SR-BI Antibody (NB400-104R) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow.

Reviews using NB400-104R:
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Images Ratings Applications Species Date Details
Flow Cytometry SR-BI NB400-104R
reviewed by:
Flow Human 04/28/2014


ApplicationFlow Cytometry
Sample Testedhuman adipose tissue macrophages


Blocking Details10% bovine serum in PBS

Primary Anitbody

Dilution RatioSR-B1


Detection NotesLSRII flow cytometer
Fixation Detailsfreshly isolated cells by collagenase digestion
Permeabilization Detailsnot applicable
Wash Descriptionsame as above

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-104R) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

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Research Areas for SR-BI Antibody (NB400-104R)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol SCARB1

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