SR-BI Antibody

Images

 
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-101] - Scavenger receptor class B member 1, SCARB1 (SR-B1) was detected in immersion fixed paraffin-embedded sections of human liver using rabbit anti-human antibody ...read more
Simple Western: SR-BI Antibody [NB400-101] - Simple Western lane view shows a specific band for SR-BI in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Western Blot: SR-BI Antibody [NB400-101] - Detection of SR-BI in mouse liver lysate (20 ug) using NB 400-101. ECL detection 5 seconds.
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-101] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and ...read more
Flow (Intracellular): SR-BI Antibody [NB400-101] - An intracellular stain was performed on HeLa cells with SR-BI Antibody NB400-101AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and ...read more
Flow Cytometry: SR-BI Antibody [NB400-101] - SR-BI antibody was tested at 1:400 in HeLa cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChHa, Md, Pm, SjSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SR-BI Antibody Summary

Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt# Q61009]
Localization
Cell Membrane; multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 1:100
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 2.5-5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5-5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Block/Neutralize
  • Flow (Intracellular)
Application Notes
This SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.Blocking/Neutralizing was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24244566)

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Liver Lysate (NB820-59662)
Control Peptide
SR-BI Peptide (NB400-101PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB400-101 in the following applications:

Publications
Read Publications using
NB400-101 in the following applications:

  • B/N
    3 publications
  • 6 publications
  • 2 publications
  • IB
    1 publication
  • 8 publications
  • IHC
    2 publications
  • 2 publications
  • IP
    2 publications
  • WB
    22 publications

Reactivity Notes

Human, mouse, mink, primate, rat, Chinese Hamster and Schistosoma japonicum. bovine reactivity reported in scientific literature (PMID: 24196350)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SR-BI Antibody

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to CD36 family and act as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and HDL located in caveolae (regions over plasma membrane). It plays important role in meditating the uptake of HDL-derived cholesterol as well as cholesteryl ester in liver and steroidogenic tissues. It facilitates the flux of free and esterified cholesterol between cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. SR-BI is expressed in endothelial cells, macrophages and dendritic cells also. It has been suggested to be involved in the phagocytosis of apoptotic cells (via its phosphatidylserine binding activity); uptake of lipid soluble vitamins (vitamin E and carotenoids) and pathogen recognition, anti-inflammatory responses and its expression can be modulated by LPS as well as ROS. SR-BI has also been involved in the capture and cross-presentation of antigens from viruses, bacteria and parasites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt, ChHa, Ha, Mk, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P, In vitro, Flow-IC
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Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-IC
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NB400-101
Species: Hu, Mu, Rt, Bv, ChHa, Md, Pm, Sj
Applications: WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N

Publications for SR-BI Antibody (NB400-101)(89)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Chinese Hamster, Primate, S. japonicum.

We have publications tested in 9 applications: B/N, FLOW, Flow, IB, ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
B/N
(3)
FLOW
(6)
Flow
(2)
IB
(1)
ICC/IF
(8)
IHC
(2)
IHC-Fr
(2)
IP
(2)
WB
(22)
All Applications
Filter By Species
Human
(18)
Mouse
(17)
Rat
(4)
Bovine
(1)
Chinese Hamster
(1)
Primate
(2)
S. japonicum
(1)
All Species
Showing Publications 1 - 10 of 89. Show All 89 Publications.
Publications using NB400-101 Applications Species
Conlon Dm, Thomas T, Fedotova T et al. Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis. J. Clin. Invest. Sep 6 2016 [PMID: 27599291] (IB) IB
Pasterk L, Lemesch S, Leber B et al. Oxidized plasma albumin promotes platelet-endothelial crosstalk and endothelial tissue factor expression Sci Rep Feb 24 2016 [PMID: 26905525] (B/N, Human) B/N Human
Vaisman BL, Vishnyakova TG, Freeman LA et al. Endothelial Expression of Scavenger Receptor Class B, Type I Protects against Development of Atherosclerosis in Mice. Biomed Res Int 2015 [PMID: 26504816] (WB, Mouse) WB Mouse
Robert J, Weber B, Frese L et al. A Three-Dimensional Engineered Artery Model for In Vitro Atherosclerosis Research. PLoS One. 2013 Nov 14 [PMID: 24244566] (IHC-Fr, ICC/IF, Human) IHC-Fr, ICC/IF Human
Mao D, Hou X, Talbott H et al. ATF3 Expression in the Corpus Luteum: Possible Role in Luteal Regression{dagger}. Mol Endocrinol. 2013 Dec [PMID: 24196350] (WB, Bovine) WB Bovine
Hofmann M. Influence of the Maillard reaction on the allergenicity of food allergens. Thesis. 2014 (Flow, Human)

Details:
Citation using the DyLight 488 form of this antibody.
Flow Human
Hofmann M. Influence of the Maillard reaction on the allergenicity of food allergens Thesis 2014 (FLOW, Human)

Details:
This reference used the DyLight 488 version of NB400-101.
FLOW Human
Maddaloni E, Xia Y, Park K et al. High density lipoprotein modulates osteocalcin expression in circulating monocytes: a potential protective mechanism for cardiovascular disease in type 1 diabetes Cardiovasc Diabetol 2017 Sep 16 [PMID: 28915881] (Flow, Human) Flow Human
Hofmann M. Influence of the Maillard reaction on the allergenicity of food allergens. Thesis. 2014 (FLOW, Human) FLOW Human
Pinto P, da Silva K, Iborra R et al. Exercise Training Favorably Modulates Gene and Protein Expression That Regulate Arterial Cholesterol Content in CETP Transgenic Mice Front. Physiol. May 8 2018 [PMID: 29867549] (WB, Mouse) WB Mouse
Show All 89 Publications.

Review for SR-BI Antibody (NB400-101) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB400-101:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SR-BI NB400-101
Enlarge
reviewed by:
Corey.
WB Human 04/09/2013
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsTested in human. Refer to PDF for protocol details.
Pub Med ID23221398
FileView PDF

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-101). (Showing 1 - 3 of 3 FAQs).

  1. I did western blot for SR-BI using NB400-101 antibody. I just want to ask the denature condition when you test this antibody.
    • We denature all of our proteins using a standard protocol of 95 degrees for 10 minutes in 5% BME on all of our samples. Here is the rest of the Western Blot protocol used specifically for this antibody. 1. Run approximately 50 ug of protein on a 4-20% Tris-glycine mini-gel at 125V for 60 minutes. 2. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes. 3. Transfer protein to the membrane at 25V for 90 minutes. 4. Allow membrane to air-dry. 5. Block membrane with 1XPBS/5% non-fat milk/0.1% Tween-20 for 1 hour at room temperature (approx. 23-27 degrees C). 6. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05% Tween-20 (PBST). 7. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 8. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 9. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 10. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 11. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts (NB800-PC3) were used as a positive control for this antibody.
  2. Would you please help us to confirm the application of NB400-101? Can this antibody be used for IHC-P or IHC-Fr? If yes, what is the dilution for the two application? The customer finds that NB400-104 and NB400-101 have the same immunogen, but the molecular band is not the same, they are approx. 82kDa and approx. 75kDa separately. We are confused with this and would you please tell us the dilution for NB400-104 in IHC-P detection?
    • Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.
  3. What is the difference between NB400-101 and NB400-104? The same IF image is shown on both product pages, so which antibody was really used?
    • Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB400-101AF405
Alexa Fluor 488 Labeled NB400-101AF488
Alexa Fluor 647 Labeled NB400-101AF647
Alexa Fluor 700 Labeled NB400-101AF700
Biotin Labeled NB400-101B
DyLight 405 Labeled NB400-101V
DyLight 488 Labeled NB400-101G
DyLight 550 Labeled NB400-101R
DyLight 650 Labeled NB400-101C
FITC Labeled NB400-101F
HRP Labeled NB400-101H
PE Labeled NB400-101PE

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Bioinformatics Tool for SR-BI Antibody (NB400-101)

Discover related pathways, diseases and genes to SR-BI Antibody (NB400-101). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Pathways for SR-BI Antibody (NB400-101)

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Research Areas for SR-BI Antibody (NB400-101)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Recent Reviews

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1
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Corey.
04/09/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol SCARB1
Entrez
Uniprot