SR-BI Antibody

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Genetic Strategies: Knockout Validated: SR-BI Antibody [NB400-101] - Immunofluorescent analysis of SR-BI localization in aorta of Tie2-Scarb1 x Scarb1-KO and Scarb1-KO mice. Mouse aorta sections were stained for ...read more
Simple Western: SR-BI Antibody [NB400-101] - Lane view shows a specific band for SR-BI in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Genetic Strategies: Knockout Validated: SR-BI Antibody [NB400-101] - Immunofluorescent analysis of SR-BI localization in liver of normal, Tie2-Scarb1 x Scarb1-KO, and Scarb1-KO mice. Liver sections from normal ...read more
Western Blot: SR-BI Antibody [NB400-101] - Detection of SR-BI in mouse liver lysate (20 ug) using NB 400-101. ECL detection 5 seconds.
Flow (Intracellular): SR-BI Antibody [NB400-101] - An intracellular stain was performed on HeLa cells with NB400-101AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Genetic Strategies: Knockout Validated: SR-BI Antibody [NB400-101] - Western blot and Coomassie stain of membrane fractions isolated from livers of normal C57Bl/6N (lane 1), Scarb1-KO (lane 2), and LIV11-SCARB1 x ...read more
Western Blot: SR-BI Antibody [NB400-101] - Western blot analysis of ABCA1 and SR-B1 proteins from WT and Osbpl8KO mouse liver. The blots were probed with anti-beta-actin as a loading control. Densitometric ...read more
Immunocytochemistry/ Immunofluorescence: SR-BI Antibody [NB400-101] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-SR-BI at 2 ug/ml overnight at 4C and ...read more
Immunohistochemistry-Paraffin: SR-BI Antibody [NB400-101] - SR-B1 was detected in immersion fixed paraffin-embedded sections of human liver using rabbit anti-human antibody (Catalog # NB400-101) at 1:300 dilution ...read more
Western Blot: SR-BI Antibody [NB400-101] - Western blot analysis of ABCA1 and SR-B1 proteins from WT and Osbpl8KO mouse liver. The blots were probed with anti-beta-actin as a loading control. Densitometric ...read more
Western Blot: SR-BI Antibody [NB400-101] - Western blot analysis of ABCA1 and SR-B1 proteins from WT and Osbpl8KO mouse liver. The blots were probed with anti-beta-actin as a loading control. Densitometric ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChHa, Md, Pm, SjSpecies Glossary
Applications WB, Simple Western, Flow, Flow-IC, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

SR-BI Antibody Summary

Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [Uniprot: Q61009]
Localization
Cell Membrane; multi-pass membrane protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Simple Western 1:100
  • Flow Cytometry 1:400
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 2.5-5 ug/ml
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 2.5-5 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Block/Neutralize
  • Flow (Intracellular)
  • Knockout Validated
Application Notes
In Western blot a band is observed at approx. 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland. Blocking/Neutralizing was reported in scientific literature (PMID: 12119305, 26905525). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 24244566). Use in flow cytometry reported in scientific literature (PMID: 23029167). Use in immunoblotting reported in scientific literature (PMID: 27599291). In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Liver Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
SR-BI Protein (NB400-101PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB400-101 in the following applications:

Publications
Read Publications using
NB400-101 in the following applications:

  • B/N
    3 publications
  • 6 publications
  • 2 publications
  • IB
    1 publication
  • 8 publications
  • IHC
    4 publications
  • 1 publication
  • IP
    2 publications
  • WB
    28 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 24196350).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SR-BI Antibody

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SR-BI Antibody (NB400-101)(102)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Chinese Hamster, Primate, S. japonicum.

We have publications tested in 9 applications: B/N, FLOW, Flow, IB, ICC/IF, IHC, IHC-Fr, IP, WB.


Filter By Application
B/N
(3)
FLOW
(6)
Flow
(2)
IB
(1)
ICC/IF
(8)
IHC
(4)
IHC-Fr
(1)
IP
(2)
WB
(28)
All Applications
Filter By Species
Human
(19)
Mouse
(21)
Rat
(6)
Bovine
(1)
Chinese Hamster
(1)
Primate
(2)
S. japonicum
(1)
All Species
Showing Publications 1 - 10 of 102. Show All 102 Publications.
Publications using NB400-101 Applications Species
Xu H, Kaul S, Kallinger R et al. PCPE2 and SR-BI Partner to Impact Accumulation of Fat in Mice bioRxiv 2018 Apr 09 (WB, Mouse) WB Mouse
Bouillet B, Gautier T, Denimal D Et Al. Glucocorticoids impair HDL-mediated cholesterol efflux besides increased HDL cholesterol concentration - a proof of concept Eur. J. Endocrinol. Jun 1 2020 [PMID: 32570209] (WB) WB
Hofmann M. Influence of the Maillard reaction on the allergenicity of food allergens. Thesis. 2014 (Flow, Human)

Details:
Citation using the DyLight 488 form of this antibody.
Flow Human
Hofmann M. Influence of the Maillard reaction on the allergenicity of food allergens. Thesis. 2014 (FLOW, Human) FLOW Human
Baranova IN, Bocharov AV, Vishnyakova TG et al. Class B Scavenger Receptors BI and BII Protect Against LPS-induced Acute Lung Injury in Mice by Mediating LPS Clearance Infection and immunity Jun 7 2021 [PMID: 34097506]
May SC, Dron JS, Hegele RA, Sahoo D Human Variant of Scavenger Receptor BI (R174C) Exhibits Impaired Cholesterol Transport Functions Journal of lipid research Feb 9 2021 [PMID: 33577783]
Xu Y, Li Y, Jadhav K, et al. Hepatocyte ATF3 protects against atherosclerosis by regulating HDL and bile acid metabolism Nature metabolism Jan 1 2021 [PMID: 33462514]
Xu Y, Zhu Y, Bawa F et al. HepatocyteSpecific Expression of Human Carboxylesterase 1 Attenuates Diet-Induced Steatohepatitis and Hyperlipidemia in Mice Hepatol Commun Feb 20 2020 [PMID: 32258948] (WB, Mouse) WB Mouse
Xu Y, Xu Y, Zhu Y et al. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis Mol. Ther. 2019 Sep 12 [PMID: 31604677]
Motherwell JM, Anderson CR, Murfee WL. Endothelial Cell Phenotypes are Maintained During Angiogenesis in Cultured Microvascular Networks Sci Rep 2018 Apr 12 [PMID: 29651134] (IHC, Rat) IHC Rat
Show All 102 Publications.

Review for SR-BI Antibody (NB400-101) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB400-101:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SR-BI NB400-101
Enlarge
4
reviewed by:
Corey.
WB Human 04/09/2013
View

Summary

ApplicationWestern Blot
SpeciesHuman
Special ApplicationsTested in human. Refer to PDF for protocol details.
Pub Med ID23221398
FileView PDF

Product General Protocols

View specific protocols for SR-BI Antibody (NB400-101): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI Antibody (NB400-101). (Showing 1 - 4 of 4 FAQs).

  1. I did western blot for SR-BI using NB400-101 antibody. I just want to ask the denature condition when you test this antibody.
    • We denature all of our proteins using a standard protocol of 95 degrees for 10 minutes in 5% BME on all of our samples. Here is the rest of the Western Blot protocol used specifically for this antibody. 1. Run approximately 50 ug of protein on a 4-20% Tris-glycine mini-gel at 125V for 60 minutes. 2. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes. 3. Transfer protein to the membrane at 25V for 90 minutes. 4. Allow membrane to air-dry. 5. Block membrane with 1XPBS/5% non-fat milk/0.1% Tween-20 for 1 hour at room temperature (approx. 23-27 degrees C). 6. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05% Tween-20 (PBST). 7. Incubate membrane with 1:1,000 dilution of NB400-101 (anti-SR-BI), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 8. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 9. Incubate membrane with 1:10,000 dilution of goat anti-rabbit IgG-HRP (BioRad), diluted in 1XPBS/1% BSA, for 1 hour at room temperature. 10. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST. 11. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HL-60 whole cell extracts (NB800-PC3) were used as a positive control for this antibody.
  2. Would you please help us to confirm the application of NB400-101? Can this antibody be used for IHC-P or IHC-Fr? If yes, what is the dilution for the two application? The customer finds that NB400-104 and NB400-101 have the same immunogen, but the molecular band is not the same, they are approx. 82kDa and approx. 75kDa separately. We are confused with this and would you please tell us the dilution for NB400-104 in IHC-P detection?
    • Both NB400-100 and NB400104 can be used with IHC paraffin embedded sections at 2.5-5 ug/ml. With regards to the WB image, since the markers are different on those gels, a 5 kDa difference between markers would not be out of the range of possibility but as far as the immunogen is concerned, both antibodies share the same region, meanwhile please keep in mind that NB400-104 can be used in human, mouse and rat tissues but NB400-101 can only be used in human and mouse.
  3. What is the difference between NB400-101 and NB400-104? The same IF image is shown on both product pages, so which antibody was really used?
    • Regarding your inquiry, NB400-101 and NB400-104 are the same antibodies and the reason why they have different catalog numbers is, in the first stage of production one of these antibodies was purified and the other was not but now both are purified and the same.
  4. Hi. I'm interested in scarb1 antibody. Can you tell me what is the difference between NB400-101 and 104?
    • SR-BI antibody NB400-101 has been validated for Block/Neutralize, while NB400-104 has not been.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB400-101AF350
Alexa Fluor 405 NB400-101AF405
Alexa Fluor 488 NB400-101AF488
Alexa Fluor 532 NB400-101AF532
Alexa Fluor 594 NB400-101AF594
Alexa Fluor 647 NB400-101AF647
Alexa Fluor 700 NB400-101AF700
Alexa Fluor 750 NB400-101AF750
Biotin NB400-101B
DyLight 350 NB400-101UV
DyLight 405 NB400-101V
DyLight 488 NB400-101G
DyLight 550 NB400-101R
DyLight 594 NB400-101DL594
DyLight 650 NB400-101C
DyLight 680 NB400-101FR
DyLight 755 NB400-101IR
FITC NB400-101F
HRP NB400-101H
Janelia Fluor 549 NB400-101JF549
Janelia Fluor 646 NB400-101JF646
PE NB400-101PE

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Bioinformatics Tool for SR-BI Antibody (NB400-101)

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Blogs on SR-BI.

ABCA1 (ATP-binding cassette transporter A1)
The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr...  Read full blog post.

ABCA1 - The Caretaker for Cholesterol Transportation
The ATP-binding cassette transporter A1 (ABCA1) protein is a key gatekeeper for regulating intracellular cholesterol transport. It is one member of a large family of genes comprised of cAMP-dependent anion transporter cell membrane proteins. These imp...  Read full blog post.

Scavenger's Helper - SR-BI (scavenger receptor class B member 1, SCARB1)
SR-B1 belongs to the CD36 scavenger receptor family and serves as a receptor for several ligands including phospholipids, cholesterol ester, lipoproteins, phosphatidylserine, and caveolae localized HDL. It is expressed in endothelial cells, macrophage...  Read full blog post.

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Recent Reviews

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Corey.
04/09/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol SCARB1
Entrez
Uniprot