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Apolipoprotein A-I/ApoA1 Antibody

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

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Apolipoprotein A-I/ApoA1 Antibody Summary

Immunogen
Apolipoprotein A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.
Localization
Secreted
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using
NB600-609 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26360112). Specific cross-reaction with Apolipoprotein A-I from other species has not been determined Non-specific cross-reaction with other human serum proteins is negligible.

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein is derived from human plasma by density gradient ultracentrifugation and HPLC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: Apolipoprotein A-II/ApoA2 Antibody (JB70-35) [NBP2-75410] - Staining Apolipoprotein A II in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Apolipoprotein A-II/ApoA2 Antibody (JB70-35) [NBP2-75410] - Analysis of paraffin-embedded rat big intestine tissue using anti-Apolipoprotein A II antibody. Counter stained with hematoxylin.
Flow Cytometry: Apolipoprotein A-II/ApoA2 Antibody (JB70-35) [NBP2-75410] - Analysis of HepG2 cells with Apolipoprotein A II antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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Western Blot: ABCA1 Antibody [NB400-105] - Western Blot analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.
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Immunocytochemistry/Immunofluorescence: ABCA1 Antibody [NB400-105] - Untreated HepG2 cells were grown to 60% confluency, and serum starved for 24 hours prior to being fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ABCA1 at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Immunocytochemistry/Immunofluorescence: LCAT Antibody [NBP1-05950] - The LCAT antibody was tested at a 1:500 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).
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Immunocytochemistry/Immunofluorescence: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunofluorescence analysis of HepG2 cells using LPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: Lipoprotein a Antibody (4H1) [NBP2-37477] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using LPA mouse mAb with DAB staining.
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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Western blot shows human plasma and human serum. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Apolipoprotein A‑IV/ApoA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8125) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein A‑IV/ApoA4 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Publications for Apolipoprotein A-I/ApoA1 Antibody (NB600-609)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ELISA, ICC/IF, IHC.


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(2)
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Bioinformatics

Gene Symbol APOA1
Entrez
Uniprot