Apolipoprotein A-I/ApoA1 Antibody

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Apolipoprotein A-I/ApoA1 Antibody Summary

• Immunogen: Apolipoprotein A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.
• Localization: Secreted
• Specificity: Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
• Application Notes: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Immunocytochemistry/Immunofluorescence was reported in scientific literature.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody

• Alp-1
• APOA1
• Apo-AI
• apoA-I
• Apolipoprotein A1
• Apolipoprotein AI
• Apolipoprotein A-I
• Brp-14
• Ltw-1
• Lvtw-1
• MGC117399
• Sep-1
• Sep-2

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein is derived from human plasma by density gradient ultracentrifugation and HPLC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein A-I/ApoA1 Antibody (NB600-609)(5)

Publications using NB600-609

• Vu TM, Ishizu AN, Foo JC et al. Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets Nature. 2017 Oct 26 [PMID: 29045386] (Mouse)
• Lewallen DM, Bicker KL, Subramanian V et al. Chemical Proteomic Platform To Identify Citrullinated Proteins. ACS Chem. Biol. 2015 Sep 23 [PMID: 26360112] (ELISA, Human)
• Molina L, Fasquelle L, Nouvian R et al. Tmprss3 loss of function impairs cochlear inner hair cell Kcnma1 channel membrane expression Hum Mol Genet 2013 Jan 3 [PMID: 23255163] (IHC, ICC/IF, Mouse)
• Hasebe, A, Pennock, N D, Mu, H-H, Chan, F V, Taylor, M L, Cole, B C. A Microbial TLR2 Agonist Imparts Macrophage-Activating Ability to Apolipoprotein A-1. 177, 4826-4832. 2006 [PMID: 16982924]
• Duong PT et al. Characterization of nascent HDL particles microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res 47:832-843. 2006 [PMID: 16418537]

Bioinformatics

• Gene Symbol: APOA1
• Entrez:
  • 335(Human)
  • 11806(Mouse)
  • 25081(Rat)
• Uniprot:
  • Q00623(Mouse)