Apolipoprotein A-I/ApoA1 Antibody

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Apolipoprotein A-I/ApoA1 Antibody Summary

Immunogen
Apolipoprotein A-I was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification.
Localization
Secreted
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using
NB600-609 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32796843). Human reactivity reported in scientific literature (PMID: 26360112).. Specific cross-reaction with Apolipoprotein A-I from other species has not been determined Non-specific cross-reaction with other human serum proteins is negligible.

Packaging, Storage & Formulations

Storage
Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein is derived from human plasma by density gradient ultracentrifugation and HPLC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A-I/ApoA1 Antibody (NB600-609)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC, WB.


Filter By Application
ELISA
(2)
ICC/IF
(1)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(3)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB600-609 Applications Species
Tao H, Huang J, Yancey PG et al. Scavenging of reactive dicarbonyls with 2-hydroxybenzylamine reduces atherosclerosis in hypercholesterolemic Ldlr-/- mice Nat Commun Aug 14 2020 [PMID: 32796843] (WB, Human) WB Human
Biddy BA, Kong W, Kamimoto K et al. Single-cell mapping of lineage and identity in direct reprogramming Nature Dec 1 2018 [PMID: 30518857] (Mouse) Mouse
Tilvawala R, Nguyen SH, Maurais AJ et al. The Rheumatoid Arthritis-Associated Citrullinome. Cell Chem Biol Mar 21 2018 [PMID: 29628436] (ELISA) ELISA
Fan SM, Tsai CF, Yen CM et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 2018 Jun [PMID: 29567388]
Vu TM, Ishizu AN, Foo JC et al. Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets Nature. 2017 Oct 26 [PMID: 29045386] (Mouse) Mouse
Lewallen DM, Bicker KL, Subramanian V et al. Chemical Proteomic Platform To Identify Citrullinated Proteins. ACS Chem. Biol. 2015 Sep 23 [PMID: 26360112] (ELISA, Human) ELISA Human
Molina L, Fasquelle L, Nouvian R et al. Tmprss3 loss of function impairs cochlear inner hair cell Kcnma1 channel membrane expression Hum Mol Genet 2013 Jan 3 [PMID: 23255163] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Hasebe, A, Pennock, N D, Mu, H-H, Chan, F V, Taylor, M L, Cole, B C. A Microbial TLR2 Agonist Imparts Macrophage-Activating Ability to Apolipoprotein A-1. 177, 4826-4832. 2006 [PMID: 16982924]
Duong PT et al. Characterization of nascent HDL particles microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res 47:832-843. 2006 [PMID: 16418537]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol APOA1
Entrez
Uniprot