Apolipoprotein A-I/ApoA1 Antibody

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

Apolipoprotein A-I/ApoA1 Antibody Summary

• Description: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities
  
  Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Apolipoprotein A-I/ApoA1 was isolated from mouse plasma by density gradient centrifugation followed by HPLC purification. (Uniprot: Q00623)
• Localization: Secreted
• Specificity: Typically less than 1% cross-reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other mouse serum proteins is negligible.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:1000
• Application Notes: This product have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.

Reactivity Notes

This antibody reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins. Specific cross-reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross-reaction of anti-apoLipoprotein antibodies with other mouse serum proteins is negligible.
Human reactivity reported in scientific literature (PMID: 32796843).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody

• Alp-1
• APOA1
• Apo-AI
• apoA-I
• Apolipoprotein A1
• Apolipoprotein AI
• Apolipoprotein A-I
• Brp-14
• Ltw-1
• Lvtw-1
• MGC117399
• Sep-1
• Sep-2

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein is derived from human plasma by density gradient ultracentrifugation and HPLC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein A-I/ApoA1 Antibody (NB600-609)(10)

Publications using NB600-609

• Fazilaty H, BrUgger MD, Valenta T Et al. Tracing colonic embryonic transcriptional profiles and their reactivation upon intestinal damage Cell reports 2021-08-03 [PMID: 34348153] (IF/IHC, Human, Mouse)
• Tao H, Huang J, Yancey PG et al. Scavenging of reactive dicarbonyls with 2-hydroxybenzylamine reduces atherosclerosis in hypercholesterolemic Ldlr-/-mice Nat Commun 2020-08-16 [PMID: 32796843] (WB, Human)
• Biddy BA, Kong W, Kamimoto K et al. Single-cell mapping of lineage and identity in direct reprogramming Nature 2018-12-01 [PMID: 30518857] (Mouse)
• Tilvawala R, Nguyen SH, Maurais AJ et al. The Rheumatoid Arthritis-Associated Citrullinome. Cell Chem Biol 2018-03-21 [PMID: 29628436] (ELISA)
• Fan SM, Tsai CF, Yen CM et al. Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin. Biomaterials 2018-03-13 [PMID: 29567388]
• Vu TM, Ishizu AN, Foo JC et al. Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets Nature. 2017-10-26 [PMID: 29045386] (Mouse)
• Lewallen DM, Bicker KL, Subramanian V et al. Chemical Proteomic Platform To Identify Citrullinated Proteins. ACS Chem. Biol. 2015-09-23 [PMID: 26360112] (ELISA, Human)
• Molina L, Fasquelle L, Nouvian R et al. Tmprss3 loss of function impairs cochlear inner hair cell Kcnma1 channel membrane expression Hum Mol Genet 2013-01-03 [PMID: 23255163] (ICC/IF, IF/IHC, Mouse)
• Hasebe, A, Pennock, N D, Mu, H-H, Chan, F V, Taylor, M L, Cole, B C. A Microbial TLR2 Agonist Imparts Macrophage-Activating Ability to Apolipoprotein A-1. 177, 4826-4832. 2006-01-01 [PMID: 16982924]
• Duong PT et al. Characterization of nascent HDL particles microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J Lipid Res 47:832-843. 2006-01-01 [PMID: 16418537]

Bioinformatics

• Gene Symbol: APOA1
• Entrez:
  • 11806(Mouse)
• Uniprot:
  • Q00623(Mouse)