Apolipoprotein A-I/ApoA1 Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Apolipoprotein A-I/ApoA1 Antibody Summary

Immunogen
Full length native apoLipoprotein Type A-I (purified).
Localization
Secreted
Specificity
Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA.This reacts with mouse apoLipoprotein A-I and has negligible cross-reactivity with Type A-II, B, C-I, C-II, C-III, E and J apoLipoproteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOA1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:5000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:250-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:100
Application Notes
Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using
NB600-609 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.125M Sodium Borate, 75mM NaCl and 5mM EDTA, pH 8.0
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein A-I/ApoA1 Antibody

  • Alp-1
  • APOA1
  • Apo-AI
  • apoA-I
  • Apolipoprotein A1
  • Apolipoprotein AI
  • Apolipoprotein A-I
  • Brp-14
  • Ltw-1
  • Lvtw-1
  • MGC117399
  • Sep-1
  • Sep-2

Background

At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A-1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). This apo-lipoprotein is derived from human plasma by density gradient ultracentrifugation and HPLC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein A-I/ApoA1 Antibody (NB600-609)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics Tool for Apolipoprotein A-I/ApoA1 Antibody (NB600-609)

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Diseases for Apolipoprotein A-I/ApoA1 Antibody (NB600-609)

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Bioinformatics

Gene Symbol APOA1
Entrez
Uniprot