This SR-BI antibody is useful for Flow cytometry (PMID 23029167), Western blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections and Immunoprecipitation. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI such as liver, ovary and adrenals and to a lesser extent testis, heart and mammary gland.Blocking/Neutralizing was reported in scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24244566) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
82 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NB400-101G in the following applications:
Human, mouse, mink, primate, rat, Chinese Hamster and Schistosoma japonicum. bovine reactivity reported in scientific literature (PMID: 24196350)
Alternate Names for SR-BI Antibody [DyLight 488]
CD36 and LIMPII analogous 1
CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
CD36L1scavenger receptor class B type 1
CLA1 CD36 antigen-like 1
Collagen type I receptor, thrombospondin receptor-like 1
scavenger receptor class B type III
scavenger receptor class B, member 1
SRB1 scavenger receptor class B member 1
High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. The SR-BI binds HDLs and mediates selective uptake of HDL cholesteryl ester. SR-BI binds HDL with high affinity, is expressed primarily in liver and nonplacental steroidgenic tissues, and mediates selective cholesterol uptake by a distinct mechanism. In mice, it seems that SR-BI plays a key role in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for SR-BI Antibody (NB400-101G). (Showing 1 - 1 of 1 FAQ).
In the data sheet it is mentioned, that this antibody can be used for Flow Cytometry. The peptide used for generation of the antibody seems to be located in the transmembrane region and the intracellular part. To me this means that we need a protocol for intracellular staining. Unfortunately I have not found a reference dealing with it. It would be really kind of you if you could provide me with a protocol for staining of human cells for FACS analysis.
You may use our Flow Cytometry protocol. You will need to incorporate a permeabilization step in order to stain the cells with our SR-BI antibody. We recommend using cold methanol (which is described in the protocol) or 0.1%-0.5% Tween-20 to permeabilize your cells. Moreover, I would recommend you to follow the following research publications in which NB100-101 has been cited for its FLOW application: PMIDs 12761383, 15557368, 23029167, 23549085.
ABCA1 (ATP-binding cassette transporter A1) The ABCA1 molecule is a primary gatekeeper for regulating the intracellular transport of cholesterol. It belongs to a larger related multifamily of cAMP-dependent anion transporter cell membrane molecules. These key proteins are responsible for tr... Read full blog post.