SOX2 Antibody - BSA Free

Immunofluorescence:SOX2 Antibody[NBP3-31961]- staining of NTERA2 cells stained with Novus Biologicals anti-SOX2 antibody (green) showing strong nuclear staining of endogenous SOX2. Actin filaments are stained with phalloidin (red).

Western Blot:SOX2 Antibody[NBP3-31961]- Western blotting of SOX2-FLAG transfected COS-7 cell lysate (10 µg/lane), mock transfected COS-7 cell lysate (10 µg/lane) and NTERA2 cell lysate (10 µg/lane) and stained with Novus Biologicals anti-SOX2 antibody (1µg/mL).Note that SOX2 runs at higher molecular weight in lane 1 due to presence of tandem Myc/FLAG tag on recombinant protein relative to endogenous SOX2 in lane 3.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.2 mg/ml

SOX2 Antibody - BSA Free Summary

• Description: Novus Biologicals Chicken SOX2 Antibody - BSA Free (NBP3-31961) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Store at 4C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
• Immunogen: His tagged full length protein.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: SOX2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered (10 mM) isotonic (0.9%, w v) saline (PBS pH 7.2)
• Preservative: 0.02% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for SOX2 Antibody - BSA Free

• ANOP3
• MCOPS3
• MGC2413
• SOX2
• SRY (sex determining region Y)-box 2
• SRY-related HMG-box gene 2
• transcription factor SOX2
• transcription factor SOX-2

Background

The SOX-2 protein is a transcription factor that is critical for early embryogenesis and for embryonic stem cell pluripotency. The SOX-2 protein is also significant for eye development. SOX 2, also known as SRY related HMG BOX gene 2, belongs to the SOX (SRY-box containing gene) gene family. The SOX-2 protein is a transcription factor since it binds to DNA and regulates activity of other genes. All SOX family proteins share HMG box domains for DNA binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX2