SOX17 Antibody [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody ...read more
Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) ...read more
SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were ...read more
SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room ...read more
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

SOX17 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Specificity
Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX17
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1924 in the following applications:

Publications
Read Publications using
AF1924 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX17 Antibody [Unconjugated]

  • FLJ22252
  • SOX17
  • SRY (sex determining region Y)-box 17
  • SRY-related HMG-box transcription factor SOX17
  • transcription factor SOX-17
  • VUR3

Background

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX17 Antibody (AF1924)(165)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 10 applications: Bioassay, Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(6)
Functional Assay
(1)
ICC
(102)
IF
(1)
IHC
(35)
IHC-Fr
(5)
IHC-P
(2)
Immunoprecipitation
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(114)
Mouse
(48)
Rat
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 165. Show All 165 Publications.
Publications using AF1924 Applications Species
S Vallejo-Di, A Fleischer, J María Mart, A Sánchez-Gi, C Gómez-Mart, M Castresana, D Bachiller Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor Stem Cell Res, 2020;47(0):101917. 2020 [PMID: 32707489] (ICC, Human) ICC Human
J Serra-Vina, MB Sandler, E Pak, W Zheng, A Dutra, W Introne, WA Gahl, MC Malicdan Generation and characterization of four Chediak-Higashi Syndrome (CHS) induced pluripotent stem cell (iPSC) lines Stem Cell Res, 2020;47(0):101883. 2020 [PMID: 32619719] (ICC, Human) ICC Human
V Meraviglia, CH Arendzen, M Tok, C Freund, AS Maione, E Sommariva, M Bellin Generation of human induced pluripotent stem cell line LUMCi027-A and its isogenic gene-corrected line from a patient affected by arrhythmogenic cardiomyopathy and carrying the c.2013delC PKP2 mutation Stem Cell Res, 2020;46(0):101835. 2020 [PMID: 32485643] (ICC, Human) ICC Human
N Drick, J Dahlmann, A Sahabian, A Haase, G Göhring, N Lachmann, FC Ringshause, T Welte, U Martin, R Olmer Generation of two human induced pluripotent stem cell lines (MHHi017-A, MHHi017-B) from a patient with primary ciliary dyskinesia carrying a homozygous mutation (c.7915C�>�T [p.Arg2639*]) in the DNAH5 gene Stem Cell Res, 2020;46(0):101848. 2020 [PMID: 32470793] (ICC, Human) ICC Human
J Dahlmann, A Sahabian, N Drick, A Haase, G Göhring, N Lachmann, FC Ringshause, T Welte, U Martin, R Olmer Generation of two hiPSC lines (MHHi016-A, MHHi016-B) from a primary ciliary dyskinesia patient carrying a homozygous 5�bp duplication (c.248_252dup (p.Gly85Cysfs*11)) in exon 1 of the CCNO gene Stem Cell Res, 2020;46(0):101850. 2020 [PMID: 32464346] (Flow Cytometry, Human) Flow Cytometry Human
Y Nakatake, SBH Ko, AA Sharov, S Wakabayash, M Murakami, M Sakota, N Chikazawa, C Ookura, S Sato, N Ito, M Ishikawa-H, SS Mak, LM Jakt, T Ueno, K Hiratsuka, M Matsushita, SK Goparaju, T Akiyama, KI Ishiguro, M Oda, N Gouda, A Umezawa, H Akutsu, K Nishimura, R Matoba, O Ohara, MSH Ko Generation and Profiling of 2,135 Human ESC Lines for the Systematic Analyses of Cell States Perturbed by Inducing Single Transcription Factors Cell Rep, 2020;31(7):107655. 2020 [PMID: 32433964] (ICC, Human) ICC Human
Y Ueda, C Kimura-Yos, K Mochida, M Tsume, Y Kameo, T Adachi, O Lefebvre, R Hiramatsu, I Matsuo Intrauterine Pressures Adjusted by Reichert's Membrane Are Crucial for Early Mouse Morphogenesis Cell Rep, 2020;31(7):107637. 2020 [PMID: 32433954] (IHC, Mouse) IHC Mouse
K Kamata, Y Otsuka, K Imamura, A Oishi, T Kondo, M Suga, R Shibukawa, Y Okanishi, Y Sagara, K Tsukita, T Yasukawa, H Usui, K Muguruma, A Tsujikawa, H Inoue Generation of a human induced pluripotent stem cell line, BRCi004-A, derived from a patient with age-related macular degeneration Stem Cell Res, 2020;45(0):101787. 2020 [PMID: 32416577] (ICC, Human) ICC Human
K Kamata, Y Otsuka, K Imamura, A Oishi, T Kondo, M Suga, R Shibukawa, Y Okanishi, Y Sagara, K Tsukita, T Yasukawa, H Usui, K Muguruma, A Tsujikawa, H Inoue Generation of a human induced pluripotent stem cell line, BRCi005-A, derived from a Best disease patient with BEST1 mutations Stem Cell Res, 2020;45(0):101782. 2020 [PMID: 32416576] (ICC, Human) ICC Human
H Suzuki, N Egawa, T Kondo, K Imamura, T Enami, K Tsukita, M Suga, R Shibukawa, Y Okanishi, T Uchiyama, H Inoue, R Takahashi Generation of a human induced pluripotent stem cell line derived from a Parkinson's disease patient carrying SNCA duplication Stem Cell Res, 2020;45(0):101828. 2020 [PMID: 32413791] (Flow Cytometry, Human) Flow Cytometry Human
Show All 165 Publications.

Reviews for SOX17 Antibody (AF1924) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using AF1924:
Filter by Applications
IF
(3)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMIC 23658023

Details

Detection NotesSee PMIC 23658023

Comments

CommentsPublished: See PMIC 23658023
  5
reviewed by:
Anonymous
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 22613812
SpeciesHuman

Blocking

Blocking DetailsSee PMIC 22613812

Details

Detection NotesSee PMIC 22613812

Comments

CommentsPublished: See PMIC 22613812
  4
reviewed by:
Anonymous
IF Other 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 23418163
SpeciesOther

Blocking

Blocking DetailsSee PMIC 23418163

Details

Detection NotesSee PMIC 23418163

Comments

CommentsPublished: See PMIC 23418163

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOX17 Antibody (AF1924) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin IC1924A
Biotin BAF1924
NL557 NL1924R

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Contact Information

Learn the difference between western blot and simple western

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous
01/05/2015
Application: IF
Species: Human

 
Anonymous
01/05/2015
Application: IF
Species: Human

 
Anonymous
01/05/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol SOX17
Uniprot