SOX17 Antibody [Unconjugated]

Images

 
Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody ...read more
Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 μg Goat ...read more
SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were ...read more
SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room ...read more
Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) ...read more
Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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SOX17 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOX17
Asp177-Val414
Accession # Q9H6I2
Specificity
Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX18 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOX17
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4.7
using
AF1924 in the following applications:

Publications
Read Publications using
AF1924 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX17 Antibody [Unconjugated]

  • FLJ22252
  • SOX17
  • SRY (sex determining region Y)-box 17
  • SRY-related HMG-box transcription factor SOX17
  • transcription factor SOX-17
  • VUR3

Background

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2).

  1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
  2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOX17 Antibody (AF1924)(275)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Callithrix jacchus (Marmoset), Canine, Marmoset, Pan troglodytes (Chimpanzee), Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 11 applications: Bioassay, Flow Cytometry, Functional Assay, ICC, IF, IHC, IHC-Fr, IHC-P, IHC/IF, Immunoprecipitation, Western Blot.


Filter By Application
Bioassay
(1)
Flow Cytometry
(33)
Functional Assay
(1)
ICC
(150)
IF
(2)
IHC
(60)
IHC-Fr
(5)
IHC-P
(2)
IHC/IF
(1)
Immunoprecipitation
(1)
Western Blot
(15)
All Applications
Filter By Species
Human
(202)
Mouse
(64)
Rat
(1)
Callithrix jacchus (Marmoset)
(2)
Canine
(1)
Marmoset
(1)
Pan troglodytes (Chimpanzee)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 275. Show All 275 Publications.
Publications using AF1924 Applications Species
A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse Nature Communications, 2022;13(1):7860. 2022 [PMID: 36543770] (IHC, Mouse) IHC Mouse
D Shang, T Lan, Y Wang, X Li, Q Liu, H Dong, B Xu, H Cheng, R Zhou PGCLCs of human 45,XO reveal pathogenetic pathways of neurocognitive and psychosocial disorders Cell & bioscience, 2022;12(1):194. 2022 [PMID: 36457060] (IHC/IF, Human) IHC/IF Human
DC Miller, P Lisowski, S Lickfett, B Mlody, M Bünning, C Genehr, C Ulrich, EE Wanker, S Diecke, J Priller, A Prigione Generation of induced pluripotent stem cells from three individuals with Huntington's disease Stem Cell Research, 2022;65(0):102976. 2022 [PMID: 36434993] (Flow Cytometry, Human) Flow Cytometry Human
JH Song, J Choi, YJ Hong, H La, TK Hong, K Hong, JT Do Developmental Potency and Metabolic Traits of Extended Pluripotency Are Faithfully Transferred to Somatic Cells via Cell Fusion-Induced Reprogramming Cells, 2022;11(20):. 2022 [PMID: 36291134] (ICC, Mouse) ICC Mouse
MC Jaboreck, JL Lühmann, M Mielenz, F Stanke, G Göhring, U Martin, R Olmer, S Merkert Generation of two TMEM16A knockout iPSC clones each from a healthy human iPSC line, from a Cystic Fibrosis patient specific line with p.Phe508del mutation and from the gene corrected iPSC line Stem Cell Research, 2022;64(0):102918. 2022 [PMID: 36162332] (IHC, Human) IHC Human
T Steiner, A Zink, MT Henke, G Cecchetto, M Buenning, A Rossi, M Schuelke, A Prigione RNA-based generation of iPSCs from a boy carrying the mutation m.9185�T>C in the mitochondrial gene MT-ATP6 and from his healthy mother Stem Cell Research, 2022;64(0):102920. 2022 [PMID: 36137325] (IHC, Human) IHC Human
H Tomita, KM Hines, JM Herron, A Li, DW Baggett, L Xu 7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome Elife, 2022;11(0):. 2022 [PMID: 36111785] (IHC, Mouse) IHC Mouse
TXA Pham, A Panda, H Kagawa, SK To, C Ertekin, G Georgolopo, SSFA van Knippe, RN Allsop, A Bruneau, JS Chui, L Vanheer, A Janiszewsk, J Chappell, M Oberhuemer, RS Tchinda, I Talon, S Khodeer, J Rossant, F Lluis, L David, N Rivron, BP Balaton, V Pasque Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells Cell Stem Cell, 2022;29(9):1346-1365.e10. 2022 [PMID: 36055191] (ICC, Human) ICC Human
S Mukherjee, DM Luedeke, L McCoy, M Iwafuchi, AM Zorn SOX transcription factors direct TCF-independent WNT/beta-catenin responsive transcription to govern cell fate in human pluripotent stem cells Cell Reports, 2022;40(8):111247. 2022 [PMID: 36001974] (Western Blot, Human) Western Blot Human
M Alonzo, J Contreras, S Ye, H Lin, L Hernandez-, KL McBride, K Texter, V Garg, MT Zhao Characterization of an iPSC line NCHi006-A from a patient with hypoplastic left heart syndrome (HLHS) Stem Cell Research, 2022;64(0):102892. 2022 [PMID: 35987121] (Flow Cytometry, Human) Flow Cytometry Human
Show All 275 Publications.

Reviews for SOX17 Antibody (AF1924) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using AF1924:
Filter by Applications
IF
(3)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous -
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 23658023
SpeciesHuman
  5
reviewed by:
Anonymous -
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 22613812
SpeciesHuman
  4
reviewed by:
Anonymous -
IF Other 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMIC 23418163
SpeciesOther

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SOX17 Antibody (AF1924) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for SOX17 Antibody (AF1924)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

 
Anonymous -
01/05/2015
Application: IF
Species: Human

 
Anonymous -
01/05/2015
Application: IF
Species: Human

 
Anonymous -
01/05/2015
Application: IF
Species: Other

Bioinformatics

Gene Symbol SOX17
Uniprot