alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

Images

 
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 ...read more
Genetic Strategies: Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 ...read more
Genetic Strategies: alpha -Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha -Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or ...read more
alpha ‑Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) at 25 µg/mL for 3 ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow, ICC/IF, KO
Clone
189502
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated] Summary

Immunogen
Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
Specificity
Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4
using
MAB1368 in the following applications:

Publications
Read Publications using
MAB1368 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for alpha-Fetoprotein/AFP Antibody (MAB1368)(50)

We have publications tested in 4 confirmed species: Human, Mouse, Bovine, Xenograft.

We have publications tested in 6 applications: Differentiation, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
Differentiation
(1)
Flow Cytometry
(8)
ICC
(30)
IHC
(8)
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(37)
Mouse
(10)
Bovine
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using MAB1368 Applications Species
Y Luo, Y Chen, M Zhang, X Ma, D Zhu, Y Chen Generation of an induced pluripotent stem cell line GZHMCi008-A derived from a patient with SRY-positive 46,XX testicular disorder of sex development Stem Cell Research, 2021;57(0):102583. 2021 [PMID: 34710837] (Flow Cytometry, Human) Flow Cytometry Human
A Mehta, P Lu, BV Taylor, J Charleswor, AL Cook, KP Burdon, AW Hewitt, KM Young Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis Stem Cell Research, 2021;57(0):102568. 2021 [PMID: 34678664] (Flow Cytometry, Human) Flow Cytometry Human
A Cañibano-H, L Valdes-San, AB Garcia-Del, B Ponte-Zúñi, FJ Diaz-Corra, B de la Cerd Generation of the human iPSC line ESi082-A from a patient with macular dystrophy associated to mutations in the CRB1 gene Stem Cell Research, 2021;53(0):102301. 2021 [PMID: 33773389] (Flow Cytometry, Human) Flow Cytometry Human
A Haase, T Kohrn, V Fricke, ME Ricci Sign, M Witte, G Göhring, I Gruh, U Martin Establishment of MHHi001-A-5, a GCaMP6f and RedStar dual reporter human iPSC line for in vitro and in vivo characterization and in situ tracing of iPSC derivatives Stem Cell Research, 2021;52(0):102206. 2021 [PMID: 33571874] (Flow Cytometry, Human) Flow Cytometry Human
J Geuder, LE Wange, A Janjic, J Radmer, P Janssen, JW Bagnoli, S Müller, A Kaul, M Ohnuki, W Enard A non-invasive method to generate induced pluripotent stem cells from primate urine Scientific Reports, 2021;11(1):3516. 2021 [PMID: 33568724] (ICC, Human) ICC Human
S Merkert, MC Jaboreck, L Engels, MNH Malik, G Göhring, F Pessler, U Martin, R Olmer Generation of two human ISG15 knockout iPSC clones using CRISPR/Cas9 editing Stem Cell Research, 2020;50(0):102135. 2020 [PMID: 33383405] (Flow Cytometry, Human) Flow Cytometry Human
L Jin, Y Chen, DK Crossman, A Datta, T Vu, JA Mobley, MK Basu, M Scarduzio, H Wang, C Chang, PK Datta STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells Nat Commun, 2020;11(1):5941. 2020 [PMID: 33230114] (IHC, Mouse) IHC Mouse
YP Li, H Liu, ZB Jin Generation of three human iPSC lines from a retinitis pigmentosa family with SLC7A14 mutation Stem Cell Res, 2020;49(0):102075. 2020 [PMID: 33207307] (ICC, Human) ICC Human
MA Jamil, H Singer, R Al-Rifai, N Nüsgen, M Rath, S Strauss, I Andreou, J Oldenburg, O El-Maarri Molecular Analysis of Fetal and Adult Primary Human Liver Sinusoidal Endothelial Cells: A Comparison to Other Endothelial Cells Int J Mol Sci, 2020;21(20):. 2020 [PMID: 33096636] (ICC, Human) ICC Human
M Kamand, M Ilieva, S Louise For, M Thomassen, M Meyer, Å Fex Svenni, T Maria Mich Derivation of induced pluripotent stem cells (SDUKIi003-A) from a 20-year-old male patient diagnosed with Asperger syndrome Stem Cell Res, 2020;48(0):101974. 2020 [PMID: 32916638] (ICC, Human) ICC Human
Show All 50 Publications.

Reviews for alpha-Fetoprotein/AFP Antibody (MAB1368) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Pig.

Reviews using MAB1368:
Filter by Applications
ICC
(1)
IF
(2)
All Applications
Filter by Species
Human
(2)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry alpha-Fetoprotein/AFP MAB1368
Enlarge
4
reviewed by:
Verified Customer
ICC Pig 12/05/2016
View

Summary

ApplicationImmunocytochemistry
Sample Testedpig trabecular meshwork
SpeciesPig
  4
reviewed by:
Verified Customer
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsIndependent review from antibodyresource.com
  4
reviewed by:
Verified Customer
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22829291
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22829291

Details

Detection NotesSee PMID 22829291

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for alpha-Fetoprotein/AFP Antibody (MAB1368) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for alpha-Fetoprotein/AFP Antibody (MAB1368)

Discover related pathways, diseases and genes to alpha-Fetoprotein/AFP Antibody (MAB1368). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

Verified Customer
12/05/2016
Application: ICC
Species: Pig

 
Verified Customer
02/04/2015
Application: IF
Species: Human

 
Verified Customer
02/04/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol AFP