alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

Images

 
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # ...read more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 ...read more
Genetic Strategies: Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 ...read more
alpha -Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human alpha -Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody ...read more
alpha ‑Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) at 25 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, CyTOF-ready, Flow, ICC/IF, KO
Clone
189502
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated] Summary

Immunogen
Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
Specificity
Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 5 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Knockout Validated
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Reviewed Applications
Read 3 Reviews rated 4
using
MAB1368 in the following applications:

Publications
Read Publications using
MAB1368 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-Fetoprotein/AFP Antibody (189502) [Unconjugated]

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

Background

alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB1368
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, Flow, ICC/IF, KO

Publications for alpha-Fetoprotein/AFP Antibody (MAB1368)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 9 applications: Flow, ICC, ICC Fresh, IHC, IHC Frozen, IHC - Not specified, IHC - PFA fixed, IHC Not Specified, IHC Paraffin-embedded.


Filter By Application
Flow
(1)
ICC
(13)
ICC Fresh
(1)
IHC
(2)
IHC Frozen
(1)
IHC - Not specified
(1)
IHC - PFA fixed
(1)
IHC Not Specified
(1)
IHC Paraffin-embedded
(1)
All Applications
Filter By Species
Human
(14)
Mouse
(7)
Bovine
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using MAB1368 Applications Species
X Gao, JJ Yourick, RL Sprando Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel Stem Cell Res, 2018;31(0):193-196. 2018 [PMID: 30099336] (ICC,, Human) ICC Human
J Higelin, A Catanese, LL Semelink-S, S Oeztuerk, AK Lutz, J Bausinger, G Barbi, G Speit, PM Andersen, AC Ludolph, M Demestre, TM Boeckers NEK1 loss-of-function mutation induces DNA damage accumulation in ALS patient-derived motoneurons Stem Cell Res, 2018;30(0):150-162. 2018 [PMID: 29929116] (ICC,, Human) ICC Human
A Bolinches-, D Lukovic, AA Castro, M León, K Kamenarova, R Kaneva, P Jendelova, F Blanco-Kel, C Ayuso, M Cortón, S Erceg Generation of a human iPSC line from a patient with congenital glaucoma caused by mutation in CYP1B1 gene Stem Cell Res, 2018;28(0):96-99. 2018 [PMID: 29453128] (Flow,, Human) Flow Human
Y Tanaka, T Sone, N Higurashi, T Sakuma, S Suzuki, M Ishikawa, T Yamamoto, J Mitsui, H Tsuji, H Okano, S Hirose Generation of D1-1 TALEN isogenic control cell line from Dravet syndrome patient iPSCs using TALEN-mediated editing of the SCN1A gene Stem Cell Res, 2018;28(0):100-104. 2018 [PMID: 29453127] (ICC,, Human) ICC Human
J Zhang, H Cao, J Xie, C Fan, Y Xie, X He, M Liao, S Zhang, H Wang The oncogene Etv5 promotes MET in somatic reprogramming and orchestrates epiblast/primitive endoderm specification during mESCs differentiation Cell Death Dis, 2018;9(2):224. 2018 [PMID: 29445086] (ICC,, Mouse) ICC Mouse
S Merkert, C Bednarski, G Göhring, T Cathomen, U Martin Generation of a gene-corrected isogenic control iPSC line from cystic fibrosis patient-specific iPSCs homozygous for p.Phe508del mutation mediated by TALENs and ssODN Stem Cell Res, 2017;23(0):95-97. 2017 [PMID: 28925369] (ICC,, Human) ICC Human
A Schmidt, TV Woedtke, J Stenzel, T Lindner, S Polei, B Vollmar, S Bekeschus One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet Int J Mol Sci, 2017;18(4):. 2017 [PMID: 28422070] (IHC,, Mouse) IHC Mouse
Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease Sci Rep, 2016;6(0):32382. 2016 [PMID: 27660926] (IHC - Not specified,, Human) IHC - Not specified Human
Kawaguchi T, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, Imai H Generation of Naive Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors. PLoS ONE, 2015;10(8):e0135403. 2015 [PMID: 26287611] (IHC Not Specified,, Bovine) IHC Not Specified Bovine
Takenaka C, Miyajima H, Yoda Y, Imazato H, Yamamoto T, Gomi S, Ohshima Y, Kagawa K, Sasaki T, Kawamata S Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes. PLoS ONE, 2015;10(6):e0129855. 2015 [PMID: 26115194] (IHC - PFA fixed,, Human) IHC - PFA fixed Human
Show All 23 Publications.

Reviews for alpha-Fetoprotein/AFP Antibody (MAB1368) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Pig.

Reviews using MAB1368:
Filter by Applications
ICC
(1)
IF
(2)
All Applications
Filter by Species
Human
(2)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry alpha-Fetoprotein/AFP MAB1368
Enlarge
reviewed by:
Anonymous
ICC Pig 12/05/2016
View

Summary

ApplicationImmunocytochemistry
Sample Testedpig trabecular meshwork
SpeciesPig
 
reviewed by:
Anonymous
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23658023
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23658023

Details

Detection NotesSee PMID 23658023

Comments

CommentsIndependent review from antibodyresource.com
 
reviewed by:
Anonymous
IF Human 02/04/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22829291
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22829291

Details

Detection NotesSee PMID 22829291

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for alpha-Fetoprotein/AFP Antibody (MAB1368) (0)

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Secondary Antibodies

 

Isotype Controls

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Additional Array Products

Bioinformatics Tool for alpha-Fetoprotein/AFP Antibody (MAB1368)

Discover related pathways, diseases and genes to alpha-Fetoprotein/AFP Antibody (MAB1368). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for alpha-Fetoprotein/AFP Antibody (MAB1368)

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PTMs for alpha-Fetoprotein/AFP Antibody (MAB1368)

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Recent Reviews

4
5
0
4
3
3
0
2
0
1
0

Anonymous
12/05/2016
Application: ICC
Species: Pig

 
Anonymous
02/04/2015
Application: IF
Species: Human

 
Anonymous
02/04/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol AFP