Recombinant Human/Mouse/Rat Activin A Protein

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BG01V human embryonic stem cells were differentiated into endoderm using media supplemented with Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC). Control cells were cultured in medium without recombinant ...read more
1 μg/lane of Recombinant Human/Mouse/Rat Activin A was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 14 kDa and 24 kDa, ...read more
Recombinant Human/Mouse/Rat Activin A (Catalog # 338-AC) induces hemoglobin expression in K562 human chronic myelogenous leukemia cells. The ED50 is 0.2‑1.2 ng/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications BA

Order Details

Recombinant Human/Mouse/Rat Activin A Protein Summary

Details of Functionality
Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cells. Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The ED50 for this effect is 0.2‑1.2 ng/mL.
The specific activity of Recombinant Human/Mouse/Rat Activin A is approximately 1 IU/μg, which is calibrated against human Activin A WHO International Standard (NIBSC code: 91/626).
Source
Chinese Hamster Ovary cell line, CHO-derived
Gly311-Ser426
Accession #
N-terminal Sequence
Gly311
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
INHBA
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
14 kDa, reducing conditions
24 kDa, non-reducing conditions
Publications
Read Publications using
338-AC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100-500 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat Activin A Protein

  • Activin A
  • INHBA

Background

Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( beta A, beta B, beta C, and beta E in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two beta A chains. The beta A subunit can also heterodimerize with a beta B or beta C subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human beta A chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat beta A. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell‑associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the beta A subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha 2-Macroglobulin, Follistatin, and FLRG (15, 16).

Publications for (212)

We have publications tested in 12 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Goat, N/A, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Sheep, Xenopus.

We have publications tested in 6 applications: Bioassay, ELISA Standard, In Vivo, Surface Plasmon Resonance, cell culture, differentiation.


Filter By Application
Bioassay
(201)
ELISA Standard
(1)
In Vivo
(3)
Surface Plasmon Resonance
(2)
cell culture
(1)
differentiation
(1)
All Applications
Filter By Species
Human
(138)
Mouse
(56)
Rat
(4)
Bovine
(1)
Chicken
(1)
Goat
(1)
N/A
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Sheep
(1)
Xenopus
(3)
All Species
Showing Publications 1 - 10 of 212. Show All 212 Publications.
Publications using 338-AC Applications Species
K Sakamoto, Y Kanematsu-, Y Kamada, M Oka, T Ohnishi, M Miwa, T Asami, H Inooka Identification of ligand-selective peptidic ActRIIB-antagonists using phage display technology Biochem Biophys Rep, 2017;11(0):33-39. 2017 [PMID: 28955765] (Bioassay, Human) Bioassay Human
N Merienne, G Vachey, L de Longpre, C Meunier, V Zimmer, G Perriard, M Canales, A Mathias, L Herrgott, T Beltramine, A Maulet, T Dequesne, C Pythoud, M Rey, L Pellerin, E Brouillet, AL Perrier, R du Pasquie, N Déglon The Self-Inactivating KamiCas9 System for the Editing of CNS Disease Genes Cell Rep, 2017;20(12):2980-2991. 2017 [PMID: 28930690] (Bioassay, Human) Bioassay Human
M Ito, S Sun, T Fukuhara, R Suzuki, M Tamai, T Yamauchi, K Nakashima, YI Tagawa, S Okazaki, Y Matsuura, T Wakita, T Suzuki Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation Oncotarget, 2017;8(33):53899-53915. 2017 [PMID: 28903311] (Bioassay, Human) Bioassay Human
ZQ He, BL Xia, YK Wang, J Li, GH Feng, LL Zhang, YH Li, HF Wan, TD Li, K Xu, XW Yuan, YF Li, XX Zhang, Y Zhang, L Wang, W Li, Q Zhou Generation of Mouse Haploid Somatic Cells by Small Molecules for Genome-wide Genetic Screening Cell Rep, 2017;20(9):2227-2237. 2017 [PMID: 28854370] (Bioassay, Mouse) Bioassay Mouse
KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors Nat Commun, 2017;8(1):331. 2017 [PMID: 28835709] (Bioassay, Human) Bioassay Human
NG Kooreman, PE de Almeida, JP Stack, RV Nelakanti, S Diecke, NY Shao, RJ Swijnenbur, V Sanchez-Fr, E Matsa, C Liu, AJ Connolly, JF Hamming, PHA Quax, MA Brehm, DL Greiner, LD Shultz, JC Wu Alloimmune Responses of Humanized Mice to Human Pluripotent Stem Cell Therapeutics Cell Rep, 2017;20(8):1978-1990. 2017 [PMID: 28834758] (Bioassay, Human) Bioassay Human
S Merfeld-Cl, H Lu, X Wu, KL March, DO Traktuev Hypoxia-induced activin A diminishes endothelial cell vasculogenic activity J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28834227] (Bioassay, Human) Bioassay Human
J Xing, Y Cao, Y Yu, H Li, Z Song, H Yu In Vitro Micropatterned Human Pluripotent Stem Cell Test (�P-hPST) for Morphometric-Based Teratogen Screening Sci Rep, 2017;7(1):8491. 2017 [PMID: 28819231] (Bioassay, Human) Bioassay Human
NJ Palpant, Y Wang, B Hadland, RJ Zaunbreche, M Redd, D Jones, L Pabon, R Jain, J Epstein, WL Ruzzo, Y Zheng, I Bernstein, A Margolin, CE Murry Chromatin and Transcriptional Analysis of Mesoderm Progenitor Cells Identifies HOPX as a Regulator of Primitive Hematopoiesis Cell Rep, 2017;20(7):1597-1608. 2017 [PMID: 28813672] (Bioassay, Human) Bioassay Human
Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura Insulin-producing cells derived from &#039;induced pluripotent stem cells&#039; of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes J Diabetes Investig, 2017;0(0):. 2017 [PMID: 28796422] (Bioassay, Human) Bioassay Human
Show All 212 Publications.

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Transforming Growth Factor beta Signaling in Stem Cells
Transforming growth factor beta (TGF beta) signaling along with its family members have been implicated in development and maintenance of various organs. Stem cells are important contributors to this process and are characterized by their ability to s...  Read full blog post.

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Bioinformatics

Gene Symbol INHBA
Entrez
Uniprot