SOX18 Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HUVEC human umbilical vein endothelial cells, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human SOX18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5077) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX18 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SOX18 Antibody Summary

• Immunogen: E. coli-derived recombinant human SOX18
  Ala276-Gly384
  Accession # P35713
• Specificity: Detects endogenous human SOX18 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SOX18
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX18 Antibody

• HLTS
• SOX18
• SRY (sex determining region Y)-box 18
• SRY-box 18
• transcription factor SOX-18

Background

SOX18 (SRY-related HMG box) is a 41 kDa (predicted) Group F member of the SOX family of DNA-binding proteins. Human SOX18 is 384 amino acids (aa) in length and contains an N-terminal DNA-binding HMG box (aa 85‑153), a central TAD region (aa 168‑260) and a highly charged sequence that contains a nine aa transactivation motif (aa 323‑331). SOX18 is expressed by endothelium and vascular smooth muscle, and apparently promotes the formation and organization of new capillaries. Over aa 276‑384, human SOX18 shares 90% and 89% aa identity with mouse and porcine SOX18, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOX18
• Uniprot:
  • P35713()