SOX17 Antibody [Allophycocyanin]

Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Addition of DMSO to Activin A/low serum-based medium increases the efficiency of definitive endoderm differentiation.(A) Flow cytometry analysis of hESC and DE cells double-stained for the pluripotency marker OCT4 and the endoderm marker SOX17. Pluripotency and differentiation status in all culture conditions tested are shown as contour plots. Only upon addition of DMSO (0.5%, 1%) to Activin A-driven differentiation of hESC was the number of OCT4-positive cells significantly down regulated. Culture with 1% of DMSO yielded the highest number of SOX17+, OCT4- cells. ‘Null’ represents spontaneous differentiation with no differentiating growth factors/reagents added. Each contour plot represents 3 biological replicates pooled together. (B) Immunofluorescence staining of cells at day 2 of DE formation for OCT4 and SOX17 markers with DAPI nuclear staining. Cells cultured in Activin A alone were clearly separated into two widespread populations of SOX17- or OCT4-positive cells (B-A). DMSO in the absence of Activin A does not prime cells to acquire endoderm identity (B-B). Addition of 0.5% of DMSO to Activin A-based, DE-priming medium results in significant decrease of OCT4-positive clusters of cells as early as 48h after DE differentiation was initiated (B-C). Scale bar 100μm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0117689), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Allophycocyanin

SOX17 Antibody [Allophycocyanin] Summary

• Immunogen: E. coli-derived recombinant human SOX17
  Asp177-Val414
  Accession # Q9H6I2
• Specificity: Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOX17
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOX17 Antibody [Allophycocyanin]

• FLJ22252
• SOX17
• SRY (sex determining region Y)-box 17
• SRY-related HMG-box transcription factor SOX17
• transcription factor SOX-17
• VUR3

Background

SOX17 is a member of the SOX family of transcription factors that bind DNA via a high mobility group (HMG) domain. SOX17 is suggested to play an important role in endoderm development (1, 2). In Western blot, AF1924 detects a 60 kDa band of endogenous SOX17 which is higher than predicted molecular weight but consistent with the current literature (3).

 

1. Kanai-Azuma, M. et al. (2002) Development 129:2367.
2. Katoh, M. et al. (2002) Int. J. Mol. Med. 9:153.
3. Sohn, J. et al. (2006) J. Neuroscience 26:9722.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX17 Antibody (IC1924A)(15)

Publications using IC1924A

• Juhyung Jung, Yun Chang, Gyuhyung Jin, Qing Deng, Xiaojun Lance Lian, Xiaoping Bao Chemically defined generation of human definitive hematopoietic stem and progenitor cells STAR Protocols 2023-03-01 [PMID: 36527716]
• Yun Chang, Ramizah Syahirah, Xuepeng Wang, Gyuhyung Jin, Sandra Torregrosa-Allen, Bennett D. Elzey et al. Engineering chimeric antigen receptor neutrophils from human pluripotent stem cells for targeted cancer immunotherapy Cell Reports 2022-07-19 [PMID: 35858579] (Flow Cytometry, Human)
• Seri Choi, Jiwoong Choi, Seongmin Cheon, Jihong Song, Seung-Yeon Kim, Ji-eun Kim et al. Pulmonary fibrosis model using micro-CT analyzable human PSC–derived alveolar organoids containing alveolar macrophage-like cells Cell Biology and Toxicology 2022-08-01 [PMID: 35267148]
• Grace A. Christopher, Rebecca J. Noort, Jessica L. Esseltine Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification Biomolecules 2021-12-22 [PMID: 35053163]
• J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li Repair of acute liver damage with immune evasive hESC derived hepato-blasts Stem Cell Res, 2020-09-26;49(0):102010. 2020-09-26 [PMID: 33011360] (ICC, IHC, Human, Mouse)
• Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 2020-09-01 [PMID: 32341565]
• N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency Stem Cell Reports, 2019-11-07;13(6):1083-1098. 11/7/2019 [PMID: 31708477]
• D Sheyn, S Ben-David, W Tawackoli, Z Zhou, K Salehi, M Bez, S De Mel, V Chan, J Roth, P Avalos, JC Giaconi, H Yameen, L Hazanov, D Seliktar, D Li, D Gazit, Z Gazit Human iPSCs can be differentiated into notochordal cells that reduce intervertebral disc degeneration in a porcine model Theranostics, 2019-10-12;9(25):7506-7524. 2019-10-12 [PMID: 31695783] (Flow Cytometry, Human)
• J Lu, A Baccei, E Lummertz d, C Guillermie, S McManus, LA Finney, C Zhang, ML Steinhause, H Li, PH Lerou Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm Stem Cell Res, 2018-01-31;28(0):48-55. 2018-01-31 [PMID: 29427839] (Flow Cytometry, Human)
• Yijie Geng, Bradley Feng Mesendogen, a novel inhibitor of TRPM6, promotes mesoderm and definitive endoderm differentiation of human embryonic stem cells through alteration of magnesium homeostasis Heliyon 2015-12-05 [PMID: 26705539]
• Czysz , Katherin, Minger , Stephen, Thomas , Nick DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation. PLoS ONE, 2015-02-06;10(2):e0117689. 2015-02-06 [PMID: 25659159] (Flow Cytometry, Human)
• Wong , Amy P, Chin , Stephani, Xia , Sunny, Garner , Jodi, Bear , Christin, Rossant , Janet Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. Nat Protoc, 2015-02-05;10(3):363-81. 2015-02-05 [PMID: 25654755] (Flow Cytometry, Human)
• Takeuchi , Hiroki, Nakatsuji , Norio, Suemori , Hirofumi Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture. Sci Rep, 2014-03-27;4(0):4488. 2014-03-27 [PMID: 24671046] (Flow Cytometry, Human)
• Amanda L. Kauffman, Alexandra V. Gyurdieva, John R. Mabus, Chrissa Ferguson, Zhengyin Yan, Pamela J. Hornby Alternative functional in vitro models of human intestinal epithelia Frontiers in Pharmacology 2013-05-17 [PMID: 23847534]
• Bernardo AS, Faial T, Gardner L, Niakan KK, Ortmann D, Senner CE, Callery EM, Trotter MW, Hemberger M, Smith JC, Bardwell L, Moffett A, Pedersen RA BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages. Cell Stem Cell, 2011-08-05;9(2):144-55. 2011-08-05 [PMID: 21816365] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SOX17
• Uniprot:
  • Q9H6I2()