Anti-SOX17 Antibodies: Novus Biologicals

AF1924

SOX17 Antibody [Unconjugated]

(3 Reviews)

100 μg

AF1924

HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for SOX17 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR." class="big_lightbox">

<b>Biological Strategies Validation. </b>Endoderm-differentiated D3 mouse embryonic stem cell line was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX17/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) or control antibody (<a class=

AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. Thep21promoter was detected by standard PCR." class="big_thumb" />

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

SOX17 was detected in immersion fixed B16 mouse melanoma cell line using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

SOX17 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; <a class=

NL001) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of BG01V human embryonic stem cells untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 59 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line untreated (-) or endoderm differentiated (+), loaded at 0.2 mg/mL. A specific band was detected for SOX17 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for SOX17 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, OVCAR‑3 human ovarian carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human SOX17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for SOX17 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

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<b>Biological Strategies Validation. </b>af1924_human-sox17-affinity-purified-polyclonal-ab-212024165016.jpg

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<b>Biological Strategies Validation. </b>af1924_human-sox17-affinity-purified-polyclonal-ab-21202416503.jpg

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Characterization of hybrid cells.(A) Both ES-pNSC and pES-NSC hybrid cells are positive for alkaline phosphatase staining (100 ×). (B) RT-PCR analysis of Oct4, Nanog, Sox2, and Nestin expression in fusion partner and reprogrammed hybrid cells. Pluripotency markers, Oct4 and Nanog, which were not expressed in NSCs and pNSCs were expressed in GFP+ fusion hybrid cells. On the other hand, Nestin, which was expressed in NSCs and pNSCs was silenced after forming GFP+ fusion hybrid cells. (C) Immunocytochemistry analysis of Oct4 and Nanog in ES-pNSC and pES-NSC hybrid cells (100 ×). (D)In vitro differentiation of ES-pNSC and pES-NSC hybrid cells into ectoderm (Tuj1), mesoderm (SMA), and endoderm (Sox17) lineages (200 ×). (E) In vivo differentiation potential of ES-pNSC and pES-NSC hybrid cells through teratoma assay. These hybrid cells were contributed to secretory epithelium (ectoderm), cartilage (mesoderm) and gut epithelium (endoderm), which were stained with PAS, Asian blue, and hematoxylin eosin, respectively. Each tissue was indicated by arrow head. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0156491), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro and in-TiPSCs.(A): Immunofluorescence staining for Sox17 (endodermal marker), alpha SMA (mesodermal marker), and Nestin (ectodermal marker) in each TiPSCs1-derived differentiated cell in vitro. (B): Gross morphology of representative teratomas derived from TiPSCs1 in vivo (hematoxylin and eosin staining). Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0097397), licensed under a CC-BY license. Not internally tested by R&D Systems.

Activation of Sox17 at onset of EC regeneration and Sox17-mediated Cyclin E1 expression. a qPCR analysis of gene expression in sorted CD31+ cells from mTmG-Scl mice before and after injury (12 mg/kg i.p.). Sox17, Vegfr2, and Ccne1 increased significantly at day 2 post-LPS compared to baseline. n = 3. Color scale: the fold change increases from red to white to green color. b Western blot analysis in fresh isolated ECs from wild-type mice and quantification c showed a 5-fold increase in Sox17 protein expression within 1 day following injury compared to baseline and followed by recovery within 3 days post-LPS. n = 3. d, e Western blot analysis of cultured HLMVECs in which Sox17 was overexpressed showed 2.5x fold increase in Cyclin E1 protein expression relative to control cells. n = 3. OE, overexpression. f Representation of the CCNE1 promoter region with Sox17 binding sites (circled numbers) and their sequences. g HLMVECs were retrovirally transduced with Sox17 or control plasmid for 3 days, and Ch-IP assay followed by qPCR was performed to amplify Sox17 binding sites in the CCNE1 promoter. n = 3. h 293T cells were transfected with a Sox17 overexpression plasmid containing CCNE1 luciferase reporter constructs. Luciferase values were normalized to Renilla luciferase control reporter values. A schematic representation of corresponding deletion constructs is presented in the right panel. n = 3 and duplicates per sample. **P < 0.01 and ***P < 0.001. Data are shown as mean ± SEM. Analysis was performed using one-way ANOVA for (c) and two-way ANOVA with Bonferroni post-tests for (e, g, h) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31073164), licensed under a CC-BY license. Not internally tested by R&D Systems.

Generation and characterization of systemic lupus erythematosus (SLE)‐specific human‐induced pluripotent stem cells (hiPSCs). (A) Dermal fibroblasts derived from patient with SLE were reprogrammed into iPSCs using Sendai virus vectors and three clones (#1, #2 and #3) were characterized. RT‐PCR confirms the loss of transgenes in hiPSCs‐SLE (lanes 1, 2 and 3), presence (lane 4) in infected fibroblasts (ipF‐SLE) and absence of Sendai viral transgenes in parental fibroblasts (pF‐SLE) (lane 5). Full‐length gels are presented in File S2. (B) The hiPSCs‐SLE colonies expressed alkaline phosphatase. Scale bar, 500 μm. (C) RT‐qPCR analysis of pluripotency genes OCT4, NANOG, SOX2 and REX1 was performed in fibroblasts and in hiPSCs derived from patient with SLE. All expression values are normalized to GAPDH and relative donor fibroblasts. Data are mean ± SEM and all statistical analysis was made between hiPSCs‐SLE clones and relative fibroblasts by Student's t test showing P‐values ≤ .05 in each comparison. (D) PluriTest assays combines novelty score (blue) on x‐axis and pluripotency score (red) on y‐axis. hiPSCs‐SLE localize in the red cloud suggesting the empirical distribution of pluripotent cells compared to non‐pluripotent blue cloud. (E) Representative images of M‐FISH staining show normal karyotypes of hiPSCs‐SLE clones. (F) Immunofluorescence analysis of pluripotent stem cell markers Nanog (green), Oct4 (red) and co‐staining with DAPI (blue) in hiPSCs‐SLE. Scale bar, 50 μm. (G) Representative images of floating and adherent EBs derived from hiPSCs‐SLE at differentiation day 8 and 18, respectively. Scale bar, 500 μm. (H) RT‐qPCR results confirm the capability of hiPSCs‐SLE to differentiate into all three germ layers. The expression levels of GATA4, HAND1 and PAX6 in EBs are relative to undifferentiated hiPSCs. All expression values are normalized to GAPDH and relative hiPSCs. Data are mean ± SEM and all statistical analysis was made between EBs‐SLE and relative hiPSCs‐SL

soHIF-1 alpha signaling induces Sox17 expression. a MPO activity of flushed lung sample from mice challenged with LPS (12 mg/kg i.p.) for 6 and 24 h. n = 3. b Western blot analysis of wild-type mice lung before and after LPS-induced injury (12 mg/kg i.p.) and its quantification c showed that HIF-1 alpha protein expression increased within 6 h post-LPS and remained increased until day 2. n = 3. d Western blot analysis in freshly isolated ECs from Hif1afl/fl mice and quantification e showed significantly increased Sox17 protein expression after injury when compared to baseline levels. n = 3. f Western blot analysis in freshly isolated ECs from Hif1aEC−/− mice and quantification g showed no significant difference in Sox17 protein expression before and after injury. n = 3. **P < 0.01 and ***P < 0.001. Data are shown as mean ± SEM. Analysis was performed using one-way ANOVA for (b, c, e, g). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31073164), licensed under a CC-BY license. Not internally tested by R&D Systems.

PGCLCs derived from hc-iPSCs.Immunofluorescent staining of (A) BLIMP1 (green color) and (B) OCT4 (green color) in 4-day-old EBs cultured with PGC induction medium on hc-iPSC-1, -2, -5 and -6 (third to fifth column), PBMC derived iPSC0102 and iPSC0207 (right two column). H9 hESCs (left column) adapted in 4i medium was also detected for BLIMP1 and OCT4 antibodies. Noted that there was no BLIMP1 signals detected in undifferentiated H9 in 4i condition. All samples were doubly stained with SOX17 antibody (red color) sequentially. DAPI was used for counterstain (blue). Scale bar = 50μm. (C) Quantification of immunofluoresent staining of PGCLCs. BLIMP1/SOX17 and OCT4/SOX17 double positive cells were counted for PGCLCs and normalized with the number of DAPI. Percentage of PGCLCs per EB of all lines was presented and significance was compared with control hESC H9. ** indicates P<0.005. *** indicates P<0.001. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0165715), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Activin-A levels during Day 0–1 modulate CM vs. DE differentiation.Pluripotent H1 ESCs were induced by changing medium to RPMI/B27 (no insulin), including CHIR (7.5 μmol/L) during Day 0–1 and IWP (5 μmol/L) during Days 3–5. Activin-A was included at the indicated levels during Day 0–1. Insulin (4,000 ng/ml) was included after Day 7. Panel A, a-e shows cells double-immunostained on Day 5 for Oct4 (red) and Sox17 (green); e is a positive control wherein cells were induced to DE with Activin-A (50 ng/ml) and Bmp4 (10 ng/ml) during Days 0–5. Panel A f-i shows cells immunostained with MF20 mAb on Day 14 to detect cardiomyocytes. Panel B depicts the effect of Activin-A levels during Day 0–1 on cardiomyocyte differentiation at Day 14, determined by flow cytometry using anti-cTnT. Cultures treated with 10 ng/ml Activin-A began to rhythmically contract at Day 6. Cells treated with 50 or 100 ng/ml Activin-A did not beat at any time. Bars indicate the average values combined from multiple experiments. Vertical lines = ±SEM. P-values were calculated by Student’s t-test. The p-value over the bar denoting 10 ng/ml Activin-A is relative to cells treated with CHIR only (0 ng/ml Activin-A), whereas the p-values over the bars denoting 50 and 100 ng/ml Activin-A are relative to cells treated with 10 ng/ml Activin-A. The size bar in Aa, which pertains to panels a-i, = 200 μm. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0118670), licensed under a CC-BY license. Not internally tested by R&D Systems.

Features of ESC-INTPSCs intermediate between ESCs and EpiSCs.(A) Comparison of gene expression in ESCs, EpiSCs, and ESC-INTPSCs. (B) The number of AP-positive colonies formed in the LIF/2i condition four days after 1000 cells were seeded per dish. (C) Proliferation of ESCs, EpiSCs, and ESC-INTPSCs with or without JAKi. (D) Expression of ESRRB and FOXA2. White arrows indicate ESRRB- and FOXA2-double–positive cells. Scale bar, 100 µm. (E) Percentage of ESRRB-positive or ESRRB- and FOXA2-double–positive cells in ESCs, ESC-INTPSCs, and EpiSCs. (F) Expression of OCT3/4 and SOX17. Scale bar, 100 µm. (G) The number of Oct3/4-GFP–positive or –negative colonies derived from single cells at day 4 and 8. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0095329), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>EC specific deletion of Sox17 (Sox17EC−/−) in mice prevents endothelial regeneration. a Schematic diagram of Sox17fl/fl mice crossed with Scl-CreERT2 to delete Sox17 in ECs. After tamoxifen feeding for five days and rest for another four weeks, mice are challenged with LPS (sub-lethal 8 mg/kg, i.p.) for analysis. b Western blot analysis of Sox17 protein expression in isolated ECs obtained from flushed lungs of Sox17EC−/− and control mice. n = 4. c Quantification of b shows 80% deletion of Sox17 in ECs of Sox17EC−/− mice compared to control mice. n = 4. d Time course of lung transvascular permeability following LPS challenge in Sox17EC−/− and Sox17fl/fl mice. n = 4. While control mouse lungs showed increased endothelial permeability at day 1 post-LPS and then recovered to baseline by day 5, Sox17EC−/− mice showed prolonged endothelial barrier leakiness post-LPS. e Time course of changes in lung transvascular permeability following LPS challenge was also carried out in Sox17fl/fl mice crossed with Cdh5-CreERT2 mice. n = 4. Similar as in d, these Sox17EC−/− mice also showed persistent leakiness post-LPS while control mice fully recovered. f Survival curve of LPS challenge in Sox17EC−/− and control mice. n = 8 per group. At this sub-lethal dose, all control mice survived whereas half of Sox17EC−/− mice died on day 2 post-LPS with increased mortality on day 3. By day 5, the death rate for control mice is 0 while for Sox17EC−/− mice is 60%. g Flow cytometry analysis of CD31+CD45− ECs among whole lung population in mice following injury. n = 4. In contrast to control mice in which CD31+CD45− EC population gradually recovered with day 3 post-LPS after initial loss of ECs, Sox17EC−/− mice showed significantly delayed restoration of ECs post-LPS period. h Quantification of BrdU+ nuclei in each field of 425 μm2 area in flushed lung cryo-sections from mice following injury. n = 4 mice per group and 6 replicates per sample. Slides are co-stained with CD31-AF488, BrdU-APC, and DA

<b>Biological Strategies Validation. </b>p38-Mapk14/11 inhibition during blastocyst maturation blocks PrE differentiation/maturation. (a) Experimental schema of p38-Mapk14/11 inhibition (+SB220025), plus vehicle control (+DMSO), and the details of antibodies used to analyse ICM cell lineage marker protein expression by immunofluorescence (IF) in late blastocysts (E4.5); Nanog and Gata4 (+DMSO n = 27, +SB220025 n = 33)—green, Nanog and Sox17 (+DMSO n = 18, +SB220025 n = 20)—red, and Nanog and Gata6 (+DMSO n = 24, +SB220025 n = 27)—blue. (b) Representative single confocal z-plane micrographs of vehicle control-treated (+DMSO) or p38-Mapk14/11 inhibited (+SB220025) late-blastocyst stage/equivalent embryos, immunofluorescently stained for indicated ICM cell lineage markers (Nanog in green and Gata4, Sox17 and Gata6 in red, plus DAPI DNA stain in blue). Examples of cells classified as TE, PrE and EPI are marked with an asterisk, arrowhead and arrow, respectively. Scale bar, 15 µm. (c) Pie charts of the relative cell lineage contribution in vehicle control (+DMSO) and p38-Mapk14/11 inhibited (+SB220025) blastocysts as judged by IF to detect the stated ICM lineage marker proteins. Blue, trophectoderm (TE); yellow, epiblast (EPI—ICM exhibiting exclusive Nanog expression); green, primitive endoderm (PrE—ICM exhibiting exclusive Gata4/6 or Sox17 expression, as appropriate); orange, uncommitted ICM cells (exhibiting co-expression of both Nanog and Gata4/6 or Sox17, as appropriate); and grey, ICM cells negative for either assayed marker. (d) Bar charts show average number of cells allocated to each specified ICM lineage, as judged by the indicated IF staining regime employed. Error bars represent s.e.m. and asterisks denote statistical significant differences in cell number between the vehicle control (+DMSO, black bars) and p38-Mapk14/11 inhibited (+SB220025, grey bars) embryo groups, according to two-tailed Student's t-test, with *p < 0.05 and **p < 0.005 confidence intervals. Yellow asterisk denotes increase in cel

<b>Biological Strategies Validation. </b>Differentiation of hESCs into definitive endoderm cells by Activin A with CHIR99021.a The relative primitive streak (BRACHYRUY, MESP1, and MIXL1) gene expression of the day 1 differentiated cells by adding Activin A (100 ng/mL) with CHIR99021 (2–5 µM) or Wnt3a (25–100 ng/mL) treatments were determined by real-time quantitative PCR (qPCR). None, no WNT signaling pathway activators were used on day 1 for PS differentiation (Activin A only). b, c After 1 day, the medium was changed to Activin A (100 ng/mL) and 1 × CTS-B27 to induce DE differentiation. The definitive endoderm (FOXA2 and SOX17) and mesoderm (MESP2 and HAND1) relative gene expression levels were determined by qPCR. d Immunofluorescence analysis of the expression of SOX17 and FoxA2 for Activin A with 2 µM CHIR99021-induced differentiated cells on day 3. e, f The expression of SOX17 and FoxA2 for Activin A with 2 µM CHIR99021-induced differentiated cells was determined by flow cytometry on day 3. Isotype control antibodies were used as controls. At a specific gene expression, datum points lacking common letters differ, p < 0.05. Data are represented as the mean ± SD. Scale bar, 100 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31601782), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>HIF-1 alpha activates transcription of Sox17. a Western blot analysis in control HLMVECs and HLMVECs for which CRISPR/Cas9 was used to delete HIFs. ECs were treated with the HIF prolyl hydroxylase inhibitor DMOG to induce HIF expression. DMOG (1 mM) increased HIF-1 alpha and HIF-2 alpha protein expression in control ECs but not in ECs lacking HIF-1 alpha or HIF-2 alpha . Induction of HIF expression was coupled to Sox17 upregulation. n = 3. b Quantification of a showed that protein expression of HIF-1 alpha and HIF-2 alpha in ECs was significantly increased by DMOG treatment. Sox17 showed a 2.5-fold increase in Sox17 expression control DMSO treated ECs. The increase in Sox17 was significantly reduced in HIF-1 alpha -deleted ECs but preserved in HIF-2 alpha -deleted ECs, indicating the importance of HIF-1 alpha in mediating Sox17 expression. n = 3. c Representation of the SOX17 promoter region with 3 HREs indicated by circled numbers, and their respective sequences are displayed. d HLMVECs were exposed to normoxia or 1% O2 (hypoxia) for 8 h. Ch-IP assay followed by qPCR was performed to amplify the HRE regions in the SOX17 promoter. Studies were performed in ECs exposed to either normoxia or hypoxia. n = 3. e 293T cells were transfected with a HIF-1 alpha overexpression plasmid containing SOX17 luciferase reporter constructs. Luciferase values were normalized to Renilla luciferase control reporter values. A schematic representation of corresponding deletion constructs is presented in the right panel. n = 3 and duplicates per sample. Results show that hypoxia activation of SOX17 HRE3 was required for Sox17 expression. **P < 0.01 and ***P < 0.001. Data are shown as mean ± SEM. Analysis was performed using two-way ANOVA with Bonferroni post-tests for (b, d, e) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31073164), licensed under a CC-BY license. Not internally tested by R&D Systems.

Concordant expression of developmental programs across organoids from four human iPSC lines. a Heatmap of expression patterns for major nephrogenesis markers across organoid differentiation time points (iPSC D0, D7, D15, and D29, averaged across four cell lines, ML protocol) and human adult kidney. Expression values were row-normalized to obtain z-scores; red color indicates positive z-scores. b Canonical (NPHS2) and data-derived (CLDN5) podocyte marker genes superimposed in tSNE plots from D15 organoids (N2 line, ML protocol). c IF staining of D15 kidney organoid (N2 line, ML protocol) for CLDN5 as a marker of early podocyte differentiation derived from the single-cell data. Additional canonical podocyte markers (NPHS1, WT1) and DAPI staining as shown. d IF staining of D29 kidney organoid (AS line, ML protocol) for SOX17 and CD31, markers of endothelial cells. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31784515), licensed under a CC-BY license. Not internally tested by R&D Systems.

Localisation of Sox17 staining in proliferative and secretory phase endometrial tissue and regulation by hormones. Within the proliferative (A) and secretory (B) endometrium, Sox17 localized to the glandular (open arrowheads) and luminal (closed arrowheads) epithelium, with staining appearing in an irregular, patchy, pattern. Smaller inset boxes show relevant IgG negative controls. Imaged at 20x magnification, scale bar = 50 μm. Treatment of endometrial luminal epithelial (ECC-1) cells with 10−8 M estrogen (estradiol)/10−7 M progesterone (medroxyprogesterone acetate) (, *p < 0.05) resulted in an upregulation of Sox17 protein, when compared to untreated, estradiol only (■, E) treatment groups. (C,D) Data presented as mean ± SEM, n = 5. Representative Western immunoblot shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31664088), licensed under a CC-BY license. Not internally tested by R&D Systems.

Knockdown of SOX17 results in reduced expression in endometrial luminal epithelial cells. Transfection of ECC-1 cells with a CRISPR/Cas9 SOX17 knock down plasmid resulted in decreased SOX17 expression (KD1-5) when compared to untransfected control ECC-1 cells (ECC-1) and ECC-1 cells transfected with a control plasmid (CP). Analysed by Western immunoblot, representative blot shown. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31664088), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differentiation toward posterior definitive endoderm. (a) Expression of posterior definitive endoderm (DE) marker CDX2 (green), and pluripotency markers OCT4 and NANOG (red). Cells were exposed to LY2090314 or bFGF and induced to differentiate toward PDE on Geltrex. As a control, DE cells were stained against the same antibodies. In the DE there was no CDX2 stain but a clear nuclear localization of pluripotency markers. Scalebar 200 µm. Number of biological replicates n = 5, number of technical replicates n = 2. (b) Expression of posterior definitive endoderm (DE) markers CDX2 (green) and SOX17 (red). Cells were exposed to LY2090314 or bFGF and induced to differentiate toward PDE either on Geltrex or on laminin 111. Number of biological replicates n = 2, number of technical replicates n = 3. (c) In quantitative RT-PCR the bFGF induced higher SOX17 expression than LY2090314. Data expressed as mean ± SD. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35163236), licensed under a CC-BY license. Not internally tested by R&D Systems.

Single-cell RNA sequencing data analysis in human ICMs. A PCA map of human cells from 52 embryos with graded colours indicating FGF4 and SOX17 expression levels in E5 cells and E6-E7 ICM cells (PC1 Score= 28.09%, PC2 score = 11.34%, PC3 score = 6%). b Same PCA plot as in (a) where developmental stages are represented by the indicated colours. c Same PCA plot showing ICMd and ICMp cells (see Supplementary data 8 for the list of cells and their allocation). d Spearman correlation matrix for paired expression of the 308 FGF4-correlated genes in the 258 human ICMd cells. Genes are ordered in a hierarchical tree for similarity (see Supplementary data 9 for detailed map in the vector-based PDF file). e Single cell normalized expression levels of Epi and PrE markers in ICMp (n = 88) and ICMd (FGF4+ (n = 172) and FGF4- (n = 86)) cells (Supplementary data 8); Two-sided Wilcoxon test between FGF4+ and FGF4- ICMd cells (p-values: FGF4p < 2.00 e−16; KLF17p = 5.80 e−11; NANOGp = 5.40 e−09; PRDM14p < 2.00e−16; SOX2p = 6.10e-08; TDGF1p = 2.40 e−13; GATA4p = 0.38; GATA6p = 3.10 e−12; PDGFRAp = 1.40 e−11; SOX17p = 8.00 e−08). For boxplots: The edges of the box represent the 25th and 75th quartiles. The median is represented by the central line. The whiskers extend to 1.5 times the interquartile range (25th to 75th percentile). Cells are plotted individually. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41467-022-30858-8), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1924U-100UG
Alexa Fluor 405	AF1924V-100UG
Alexa Fluor 488	AF1924G-100UG
Alexa Fluor 532	AF1924X-100UG
Alexa Fluor 594	AF1924T-100UG
Alexa Fluor 647	AF1924R-100UG
Alexa Fluor 700	AF1924N-100UG
Alexa Fluor 750	AF1924S-100UG
Alexa Fluor Plus 405	AF1924AFP405-100UG
Alexa Fluor Plus 488	AF1924AFP488-100UG
Alexa Fluor Plus 555	AF1924AFP555-100UG
Alexa Fluor Plus 594	AF1924AFP594-100UG
Alexa Fluor Plus 647	AF1924AFP647-100UG
Alexa Fluor Plus 680	AF1924AFP680-100UG
Allophycocyanin	IC1924A
Biotin	BAF1924
CoraFluor 1	AF1924CL1
DyLight 350	AF1924D
DyLight 405	AF1924E
DyLight 488	AF1924K
DyLight 550	AF1924L
DyLight 594	AF1924M
DyLight 650	AF1924W
DyLight 680	AF1924Y
DyLight 755	AF1924Z
FITC	AF1924F
HRP	AF1924H
Janelia Fluor 525	AF1924JF525
Janelia Fluor 549	AF1924I
Janelia Fluor 646	AF1924J
Janelia Fluor 669	AF1924JF669
NL557	NL1924R
PE	AF1924P
PerCP	AF1924C
mFluor Violet 450 SE	AF1924MFV450
mFluor Violet 500 SE	AF1924MFV500
mFluor Violet 610 SE	AF1924MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC1924A
Carrier Free	NL1924R
Carrier Free	AF1924-SP

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MAB1924

SOX17 Antibody (245013) [Unconjugated]

(1 Review)

(63 Publications)

100 μg

MAB1924

NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="SOX17 was detected in immersion fixed B16 mouse melanoma cell line using Mouse Anti-Human SOX17 Monoclonal Antibody (Catalog # MAB1924) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VAMP5 KO doesn’t impair the stemness of ESCs.a–d Representative western blot images of VAMP5 KO potentially impair the related markers expression in hESCs (a) and also after differentiated into mesoderm (MES) (b), endoderm (END), (c) and ectoderm (ECT) (d). Data are represented as mean ± SEM, n = 3 biological replicates in each group. Student’s two tailed t test was used. No adjustments for multiple comparisons were made, as only two groups were compared. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. e PCA of the transcriptomic comparison of VAMP5 WT and KO in hESCs and followed differentiated three germ layers, n = 3 in each cell type. f Representative heatmap of hESCs and three germ layers related markers comparison of VAMP5 WT and KO in hESCs and followed differentiated three germ layers. g Immunofluorescence staining for lineage differentiation from VAMP5 WT and KO hESCs to mesoderm derived smooth muscles cells (markers of alpha -SMA and Transgelin), endoderm derived alveolar type II cells (markers of EpCAM and SP-B), and ectoderm derived neuron cells ( beta 3 tubulin and MAP2). The results are representative of three independent experiments. Scale bars, 50 μm. KO, knock-out; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40624080), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1924U-100UG
Alexa Fluor 405	FAB1924V-100UG
Alexa Fluor 488	FAB1924G-100UG
Alexa Fluor 532	FAB1924X-100UG
Alexa Fluor 594	FAB1924T-100UG
Alexa Fluor 647	FAB1924R-100UG
Alexa Fluor 700	FAB1924N-100UG
Alexa Fluor 750	FAB1924S-100UG
Alexa Fluor Plus 405	FAB1924AFP405-100UG
Alexa Fluor Plus 488	FAB1924AFP488-100UG
Alexa Fluor Plus 555	FAB1924AFP555-100UG
Alexa Fluor Plus 594	FAB1924AFP594-100UG
Alexa Fluor Plus 647	FAB1924AFP647-100UG
Alexa Fluor Plus 680	FAB1924AFP680-100UG
Allophycocyanin	FAB1924A
Biotin	FAB1924B
CoraFluor 1	FAB1924CL1
DyLight 350	FAB1924D
DyLight 405	FAB1924E
DyLight 488	FAB1924K
DyLight 550	FAB1924L
DyLight 594	FAB1924M
DyLight 650	FAB1924W
DyLight 680	FAB1924Y
DyLight 755	FAB1924Z
FITC	FAB1924F
HRP	FAB1924H
Janelia Fluor 525	FAB1924JF525
Janelia Fluor 549	FAB1924I
Janelia Fluor 646	FAB1924J
Janelia Fluor 669	FAB1924JF669
PE	FAB1924P
PerCP	FAB1924C
mFluor Violet 450 SE	FAB1924MFV450
mFluor Violet 500 SE	FAB1924MFV500
mFluor Violet 610 SE	FAB1924MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1924-SP

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NBP2-24568

SOX17 Antibody - BSA Free

(12 Publications)

0.1 mg

NBP2-24568

Immunohistochemistry-Paraffin: SOX17 Antibody [NBP2-24568] - Human uterus (top) and testis (bottom) stained with SOX17 antibody at 10 ug/ml.

Reactivity:

Hu, Mu, Rt, Pm, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Pm, Xp

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6447A

Alt Catalog# IMG-6447A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-24568AF350
Alexa Fluor 405	NBP2-24568AF405
Alexa Fluor 488	NBP2-24568AF488
Alexa Fluor 532	NBP2-24568AF532
Alexa Fluor 594	NBP2-24568AF594
Alexa Fluor 647	NBP2-24568AF647
Alexa Fluor 700	NBP2-24568AF700
Alexa Fluor 750	NBP2-24568AF750
Alexa Fluor Plus 405	NBP2-24568AFP405
Alexa Fluor Plus 488	NBP2-24568AFP488
Alexa Fluor Plus 555	NBP2-24568AFP555
Alexa Fluor Plus 594	NBP2-24568AFP594
Alexa Fluor Plus 647	NBP2-24568AFP647
Alexa Fluor Plus 680	NBP2-24568AFP680
Allophycocyanin	NBP2-24568APC
Biotin	NBP2-24568B
CoraFluor 1	NBP2-24568CL1
DyLight 350	NBP2-24568UV
DyLight 405	NBP2-24568V
DyLight 488	NBP2-24568G
DyLight 550	NBP2-24568R
DyLight 594	NBP2-24568DL594
DyLight 650	NBP2-24568C
DyLight 680	NBP2-24568FR
DyLight 755	NBP2-24568IR
FITC	NBP2-24568F
HRP	NBP2-24568H
Janelia Fluor 525	NBP2-24568JF525
Janelia Fluor 549	NBP2-24568JF549
Janelia Fluor 646	NBP2-24568JF646
Janelia Fluor 669	NBP2-24568JF669
PE	NBP2-24568PE
PerCP	NBP2-24568PCP
mFluor Violet 450 SE	NBP2-24568MFV450
mFluor Violet 500 SE	NBP2-24568MFV500
mFluor Violet 610 SE	NBP2-24568MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-24568SS

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NBP1-47996

SOX17 Antibody (OTI2B6)

(1 Publication)

0.1 ml

NBP1-47996

Immunohistochemistry-Paraffin: SOX17 Antibody (OTI2B6) [NBP1-47996] - Human endometrium tissue using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Western Blot: SOX17 Antibody (OTI2B6) [NBP1-47996] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17.

Immunohistochemistry-Paraffin: SOX17 Antibody (OTI2B6) [NBP1-47996] - Human endometrium tissue within the normal limits using anti-SOX17 mouse monoclonal antibody. Dilution Factor: 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min.

Reactivity:

Hu, Mu, Rt, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Ca

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-74299AF350
Alexa Fluor 405	NBP2-74299AF405
Alexa Fluor 488	NBP2-74299AF488
Alexa Fluor 532	NBP2-74299AF532
Alexa Fluor 594	NBP2-74299AF594
Alexa Fluor 647	NBP2-74299AF647
Alexa Fluor 700	NBP2-74299AF700
Alexa Fluor 750	NBP2-74299AF750
Alexa Fluor Plus 405	NBP2-74299AFP405
Alexa Fluor Plus 488	NBP2-74299AFP488
Alexa Fluor Plus 555	NBP2-74299AFP555
Alexa Fluor Plus 594	NBP2-74299AFP594
Alexa Fluor Plus 647	NBP2-74299AFP647
Alexa Fluor Plus 680	NBP2-74299AFP680
Biotin	NBP2-74299B
CoraFluor 1	NBP2-74299CL1
DyLight 350	NBP2-74299UV
DyLight 405	NBP2-74299V
DyLight 488	NBP2-74299G
DyLight 550	NBP2-74299R
DyLight 594	NBP2-74299DL594
DyLight 650	NBP2-74299C
DyLight 680	NBP2-74299FR
DyLight 755	NBP2-74299IR
FITC	NBP2-74299F
HRP	NBP2-74299H
Janelia Fluor 525	NBP2-74299JF525
Janelia Fluor 549	NBP2-74299JF549
Janelia Fluor 646	NBP2-74299JF646
Janelia Fluor 669	NBP2-74299JF669
PE	NBP2-74299PE
PerCP	NBP2-74299PCP
mFluor Violet 450 SE	NBP2-74299MFV450
mFluor Violet 500 SE	NBP2-74299MFV500
mFluor Violet 610 SE	NBP2-74299MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-74299

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NBP2-24644

SOX17 Antibody

0.1 mg

NBP2-24644

Reactivity:

Hu, Mu, Po, Ch, Ma-Op, Pm

Species Glossary

Reactivity: Hu, Mu, Po, Ch, Ma-Op, Pm

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6488A

Alt Catalog# IMG-6488A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-49549

SOX17 Antibody - BSA Free

0.1 ml

NBP2-49549

Immunohistochemistry-Paraffin: SOX17 Antibody [NBP2-49549] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Immunohistochemistry-Paraffin: SOX17 Antibody [NBP2-49549] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SOX17 Antibody [NBP2-49549] - Staining of human endometrium shows strong nuclear positivity in glandular cells.

ChIP-Exo-Seq composite graph for Anti-SOX17 (NBP2-49549) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-49549-25ul

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NBP1-80362

SOX17 Antibody - BSA Free

(1 Publication)

100 ul

NBP1-80362

Flow Cytometry: SOX17 Antibody [NBP1-80362] - Researcher: Dr. Ade Kallas, University of tartu. Application: Flow Cytometry. Species + Tissue/Cell type: A. Human H9 cells and B. Human H9 cells+Na+Butyrate. Primary antibody dilution: 2ug+1x106 cells. Secondary antibody: Chicken anti rabbit-Alexa Fl

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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FAB1924AFP488

SOX17 Antibody (245013) [Alexa Fluor™ Plus 488]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 488

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AF1924AFP680

SOX17 Antibody [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Alexa Fluor Plus 680

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FAB1924AFP647

SOX17 Antibody (245013) [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 647

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FAB1924AFP594

SOX17 Antibody (245013) [Alexa Fluor™ Plus 594]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 594

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FAB1924AFP405

SOX17 Antibody (245013) [Alexa Fluor™ Plus 405]

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 405

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

SOX17 Antibodies

SOX17 Antibody [Unconjugated]

SOX17 Antibody (245013) [Unconjugated]

SOX17 Antibody - BSA Free

SOX17 Antibody (OTI2B6)

SOX17 Antibody

SOX17 Antibody - BSA Free

SOX17 Antibody - BSA Free

SOX17 Antibody (245013) [Alexa Fluor™ Plus 488]

SOX17 Antibody [Alexa Fluor™ Plus 680]

SOX17 Antibody (245013) [Alexa Fluor™ Plus 647]

SOX17 Antibody (245013) [Alexa Fluor™ Plus 594]

SOX17 Antibody (245013) [Alexa Fluor™ Plus 405]