PDX-1/IPF1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) ...read more
PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of ...read more
PDX‑1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-HumanPDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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PDX-1/IPF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Specificity
Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF2419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Unconjugated]

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDX-1/IPF1 Antibody (AF2419)(21)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation.


Filter By Application
Flow Cytometry
(5)
ICC
(11)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(20)
Rat
(1)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF2419 Applications Species
JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton Circadian Entrainment Triggers Maturation of Human In�Vitro Islets Cell Stem Cell, 2019;26(1):108-122.e10. 2019 [PMID: 31839570] (ICC, Human) ICC Human
P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced J Pathol Clin Res, 2019;5(4):248-255. 2019 [PMID: 31493350] (IHC-P, Human) IHC-P Human
K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019;28(2):382-393.e7. 2019 [PMID: 31291575] (Immunoprecipitation, Human) Immunoprecipitation Human
EA Rosado-Oli, K Anderson, JH Kenty, DA Melton YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells Nat Commun, 2019;10(1):1464. 2019 [PMID: 30931946] (Flow Cytometry, Human) Flow Cytometry Human
X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert Point mutations in the PDX1 transactivation domain impair human ?-cell development and function Mol Metab, 2019;0(0):. 2019 [PMID: 30930126] (Flow Cytometry, Human) Flow Cytometry Human
U Diekmann, H Wolling, R Dettmer, I Niwolik, O Naujok, FFR Buettner Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture Sci Rep, 2019;9(1):996. 2019 [PMID: 30700818] (Flow Cytometry, Human) Flow Cytometry Human
T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival Nat Commun, 2018;9(1):4815. 2018 [PMID: 30446643] (ICC, Human) ICC Human
H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors Stem Cell Res Ther, 2018;9(1):277. 2018 [PMID: 30359326] (ICC, Human) ICC Human
R Wei, F Yuan, Y Wu, Y Liu, K You, Z Yang, Y Chen, A Getachew, N Wang, Y Xu, Y Zhuang, F Yang, YX Li Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing Stem Cell Res, 2018;32(0):139-144. 2018 [PMID: 30278376] (ICC, Human) ICC Human
PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity Dev. Cell, 2018;44(6):679-693.e5. 2018 [PMID: 29587142] (IHC-Fr, Human) IHC-Fr Human
Show All 21 Publications.

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Biotin BAF2419

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