PDX-1/IPF1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) ...read more
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of ...read more
PDX‑1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-HumanPDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL ...read more
PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

PDX-1/IPF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Specificity
Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDX1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF2419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Unconjugated]

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDX-1/IPF1 Antibody (AF2419)(10)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: Flow, ICC, ICC Fluorescence, IHC - Not specified, IHC - PFA fixed.


Filter By Application
Flow
(2)
ICC
(5)
ICC Fluorescence
(1)
IHC - Not specified
(1)
IHC - PFA fixed
(1)
All Applications
Filter By Species
Human
(9)
Rat
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF2419 Applications Species
KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors Nat Commun, 2017;8(1):331. 2017 [PMID: 28835709] (Flow,, Human) Flow Human
Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes J Diabetes Investig, 2017;0(0):. 2017 [PMID: 28796422] (ICC,, Human) ICC Human
H Liu, D Guo, A Ruzi, Y Chen, T Pan, F Yang, J Li, K Xu, T Zhou, D Qin, YX Li Testosterone improves the differentiation efficiency of insulin-producing cells from human induced pluripotent stem cells PLoS ONE, 2017;12(6):e0179353. 2017 [PMID: 28594910] (ICC,, Human) ICC Human
J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Rep, 2017;19(2):281-294. 2017 [PMID: 28402852] (ICC,, Human) ICC Human
Claudia Davenport The Anterior-Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt- and BMP-Signaling Stem Cells, 2016;0(0):. 2016 [PMID: 27299363] (IHC - PFA fixed,, Human) IHC - PFA fixed Human
Zhao Z, Low Y, Armstrong N, Ryu J, Sun S, Arvanites A, Hollister-Lock J, Shah N, Weir G, Annes J Repurposing cAMP-modulating medications to promote beta-cell replication. Mol Endocrinol, 2014;28(10):1682-97. 2014 [PMID: 25083741] (IHC - Not specified,, Rat) IHC - Not specified Rat
Sui L, Mfopou J, Geens M, Sermon K, Bouwens L FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells. Biochem Biophys Res Commun, 2012;426(3):380-5. 2012 [PMID: 22960178] (ICC,, Human) ICC Human
Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 2012;21(6):1321-39. 2012 [PMID: 22195604] (ICC Fluorescence,, Human) ICC Fluorescence Human
Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Stem Cells, 2011;29(4):609-17. 2011 [PMID: 21308865] (Flow,, Human) Flow Human
Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12. 2006 [PMID: 16632596] (ICC,, Human) ICC Human

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Bioinformatics

Gene Symbol PDX1
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