PDX-1/IPF1 Antibody [Unconjugated]

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX-1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

PDX-1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-Human PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Differentiation of MODY3-iPSCs into pancreatic beta cells.(A) scheme of 6 step differentiation protocol (B), expression pattern of HNF1A mRNA during differentiation process, (C) Phase-contrast image of spheroids of differentiated MODY3-iPS-beta cells, (D, E) Immunocytochemistry of differentiated MODY3-iPS-beta cells for pancreatic beta cell markers (D) C-peptide, PDX1, NKX6.1 and DAPI staining, (E) HNF1A and DAPI staining. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31145732), licensed under a CC-BY license. Not internally tested by R&D Systems.

Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for PDX-1 mRNA (ACD RNAScope Probe, catalog #437088; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human PDX-1 polyclonal antibody (R&D Systems catalog # AF2419) at 1ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to islet cells and exocrine glands.

GP2 and CD142 comparative profiles. a–j Flow cytometry analyses of H1-derived day 13 PP cultures obtained using 10 mM nicotinamide (a–e, optimal differentiation) and 3.3 mM nicotinamide (f–j, suboptimal differentiation) during stage 4 of differentiation. Cells were stained with anti-GP2 and anti-CD142 and then fixed and stained for the intracellular markers PDX1 and NKX6-1. IgG controls are shown on the left of each panel. Green boxes highlight the low percentage of GP2+/PDX− cells (c–h), red box highlights the presence of CD142+PDX1− cells (j). Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; PDX1, pancreatic, and duodenal homeobox 1 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28835709), licensed under a CC-BY license. Not internally tested by R&D Systems.

GP2 and CD142 comparative profiles. a–j Flow cytometry analyses of H1-derived day 13 PP cultures obtained using 10 mM nicotinamide (a–e, optimal differentiation) and 3.3 mM nicotinamide (f–j, suboptimal differentiation) during stage 4 of differentiation. Cells were stained with anti-GP2 and anti-CD142 and then fixed and stained for the intracellular markers PDX1 and NKX6-1. IgG controls are shown on the left of each panel. Green boxes highlight the low percentage of GP2+/PDX− cells (c–h), red box highlights the presence of CD142+PDX1− cells (j). Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; PDX1, pancreatic, and duodenal homeobox 1 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28835709), licensed under a CC-BY license. Not internally tested by R&D Systems.

MAC-sorted GP2+ cells give rise to ‘ beta -like’ cells in vitro. a Flow plots showing the GP2 profile at day 13 of differentiation of H9 cells. Cells were analyzed either before MACS sorting (presort), after GP2 enrichment from a positive selection column (GP2+) or in the flow through from a depletion column. Top right plot shows NKX6-1 and PDX1 expression by flow cytometry in day 13 unsorted (presort) H9 cells. b Following MACS sorting for GP2 at day 13, cells were cultured generate beta -like cells up to day 23. Representative flow cytometry plots of NKX6-1 and C-PEPTIDE (CPEP) expression at day 23 of differentiation from either unsorted (PRESORT), enriched for GP2 using a MACS positive selection column (GP2+) or in the flow through cell population (Flow-). The bar graph shows the average percentage of NKX6-1+/C-PEPTIDE+ cells at Day 23. N = 4, error bars indicate s.e.m. **p < 0.01, One-way ANOVA. c Model depicting the in vivo and in vitro equivalent of the human multipotent pancreatic progenitor (MPC) expressing PTF1A/GP2/NKX6-1. The MPC residing at the tip of the developing human pancreas has the potential to develop into acinar (PTF1A+/GP2+) and ductal/endocrine (NKX6-1+/GP2−) progenitors. Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; D13, day 13; NKX6-1, NK6 homeobox 1; PDX1, pancreatic and duodenal homeobox 1; PTF1A, pancreas specific transcription factor 1a Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28835709), licensed under a CC-BY license. Not internally tested by R&D Systems.

GP2 and CD142 comparative profiles. a–j Flow cytometry analyses of H1-derived day 13 PP cultures obtained using 10 mM nicotinamide (a–e, optimal differentiation) and 3.3 mM nicotinamide (f–j, suboptimal differentiation) during stage 4 of differentiation. Cells were stained with anti-GP2 and anti-CD142 and then fixed and stained for the intracellular markers PDX1 and NKX6-1. IgG controls are shown on the left of each panel. Green boxes highlight the low percentage of GP2+/PDX− cells (c–h), red box highlights the presence of CD142+PDX1− cells (j). Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; PDX1, pancreatic, and duodenal homeobox 1 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28835709), licensed under a CC-BY license. Not internally tested by R&D Systems.

GP2 and CD142 comparative profiles. a–j Flow cytometry analyses of H1-derived day 13 PP cultures obtained using 10 mM nicotinamide (a–e, optimal differentiation) and 3.3 mM nicotinamide (f–j, suboptimal differentiation) during stage 4 of differentiation. Cells were stained with anti-GP2 and anti-CD142 and then fixed and stained for the intracellular markers PDX1 and NKX6-1. IgG controls are shown on the left of each panel. Green boxes highlight the low percentage of GP2+/PDX− cells (c–h), red box highlights the presence of CD142+PDX1− cells (j). Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; PDX1, pancreatic, and duodenal homeobox 1 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28835709), licensed under a CC-BY license. Not internally tested by R&D Systems.

GP2 and CD142 comparative profiles. a–j Flow cytometry analyses of H1-derived day 13 PP cultures obtained using 10 mM nicotinamide (a–e, optimal differentiation) and 3.3 mM nicotinamide (f–j, suboptimal differentiation) during stage 4 of differentiation. Cells were stained with anti-GP2 and anti-CD142 and then fixed and stained for the intracellular markers PDX1 and NKX6-1. IgG controls are shown on the left of each panel. Green boxes highlight the low percentage of GP2+/PDX− cells (c–h), red box highlights the presence of CD142+PDX1− cells (j). Abbreviations: GP2, pancreatic secretory granule membrane major glycoprotein 2; CD142, tissue factor; NKX6-1, NK6 homeobox 1; PDX1, pancreatic, and duodenal homeobox 1 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28835709), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, Dual ISH-IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PDX-1/IPF1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PDX‑1/IPF1
  Ala91-Arg283
  Accession # P52945
• Specificity: Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDX1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Unconjugated]

• Glucose-sensitive factor
• IDX1
• IDX-1GSF
• Insulin promoter factor 1
• insulin promoter factor 1, homeodomain transcription factor
• Insulin upstream factor 1
• IPF1
• IPF1pancreas/duodenum homeobox protein 1
• Islet/duodenum homeobox-1
• MODY4IUF1
• pancreatic and duodenal homeobox 1
• pancreatic-duodenal homeobox factor 1
• PDX-1
• PDX-1IPF-1
• somatostatin transcription factor 1
• Somatostatin-transactivating factor 1
• STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDX-1/IPF1 Antibody (AF2419)(74)

Publications using AF2419

• Kelley, AB;Shunkarova, M;Maestas, MM;Gale, SE;Hogrebe, NJ;Millman, JR; Controlling human stem cell-derived islet composition using magnetic sorting bioRxiv : the preprint server for biology 2024-11-21 [PMID: 39605713] (Immunohistochemistry, Mouse)
• Hogrebe, NJ;Schmidt, MD;Augsornworawat, P;Gale, SE;Shunkarova, M;Millman, JR; Depolymerizing F-actin accelerates the exit from pluripotency to enhance stem cell-derived islet differentiation bioRxiv : the preprint server for biology 2024-10-21 [PMID: 39484596] (Flow Cytometry, Western Blot, Immunocytochemistry, Human)
• Merz, S;Senée, V;Philippi, A;Oswald, F;Shaigan, M;Führer, M;Drewes, C;Allgöwer, C;Öllinger, R;Heni, M;Boland, A;Deleuze, JF;Birkhofer, F;Gusmao, EG;Wagner, M;Hohwieler, M;Breunig, M;Rad, R;Siebert, R;Messerer, DAC;Costa, IG;Alvarez, F;Julier, C;Kleger, A;Heller, S; A ONECUT1 regulatory, non-coding region in pancreatic development and diabetes Cell reports 2024-10-19 [PMID: 39427318] (Flow Cytometry, Human)
• Yang, L;Han, Y;Zhang, T;Dong, X;Ge, J;Roy, A;Zhu, J;Lu, T;Vandana, JJ;de Silva, N;Robertson, CC;Xiang, JZ;Pan, C;Sun, Y;Que, J;Evans, T;Liu, C;Wang, W;Naji, A;Parker, SCJ;Schwartz, RE;Chen, S; Human Vascularized Macrophage-Islet Organoids to Model Immune-Mediated Pancreatic ? cell Pyroptosis upon Viral Infection bioRxiv : the preprint server for biology 2024-08-06 [PMID: 39149298] (Immunocytochemistry, Human)
• Lyu, X;Rowley, MJ;Kulik, MJ;Dalton, S;Corces, VG; Regulation of CTCF loop formation during pancreatic cell differentiation Nature communications 2023-10-09 [PMID: 37813869] (ICC, Human)
• Samuel D. Pollock, Israeli M. Galicia-Silva, Mai Liu, Zoe L. Gruskin, Juan R. Alvarez-Dominguez Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors STAR Protoc 2023-09-21 [PMID: 37738117]
• Souralova, T;Hulinova, D;Jeseta, M;Ventruba, P;Hampl, A;Koutna, I; Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells Scientific reports 2023-09-12 [PMID: 37700192] (Immunocytochemistry, Human)
• Xisheng Li, Zhangjing Ma, Kathy O. Lui A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells STAR Protocols 2023-09-01 [PMID: 37392391]
• Ito, R;Kimura, A;Hirose, Y;Hatano, Y;Mima, A;Mae, SI;Keidai, Y;Nakamura, T;Fujikura, J;Nishi, Y;Ohta, A;Toyoda, T;Inagaki, N;Osafune, K; Elucidation of HHEX in pancreatic endoderm differentiation using a human iPSC differentiation model Scientific reports 2023-05-29 [PMID: 37248264] (Flow Cytometry, ICC, Western Blot, Human)
• FM Hermann, MF Kjærgaard, C Tian, U Tiemann, A Jackson, LR Olsen, M Kraft, PO Carlsson, IM Elfving, JLT Kettunen, T Tuomi, I Novak, H Semb An insulin hypersecretion phenotype precedes pancreatic beta cell failure in MODY3 patient-specific cells Cell Stem Cell, 2022-12-22;0(0):. 2022-12-22 [PMID: 36563694] (Flow Cytometry, ICC, IHC, Human, Xenograft)
• Tereza Souralova, Daniela Rehakova, Michal Jeseta, Lenka Tesarova, Jindrich Beranek, Pavel Ventruba et al. The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy International Journal of Molecular Sciences 2022-10-18 [PMID: 36293356]
• A Beucher, I Miguel-Esc, D Balboa, MG De Vas, MA Maestro, J Garcia-Hur, A Bernal, R Gonzalez-F, P Vargiu, H Heyn, P Ravassard, S Ortega, J Ferrer The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A Nature Cell Biology, 2022-10-06;0(0):. 2022-10-06 [PMID: 36202974] (IHC, Human)
• Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
• EZ Sim, T Enomoto, N Shiraki, N Furuta, S Kashio, T Kambe, T Tsuyama, A Arakawa, H Ozawa, M Yokoyama, M Miura, S Kume Methionine metabolism regulates pluripotent stem cell pluripotency and differentiation through zinc mobilization Cell Reports, 2022-07-19;40(3):111120. 2022-07-19 [PMID: 35858556] (ICC, Human)
• JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew Divergent transcriptional regulation of astrocyte reactivity across disorders Nature, 2022-05-25;0(0):. 2022-05-25 [PMID: 35614216] (IHC, Mouse)
• Haiting Ma, Esmée de Zwaan, Yang Eric Guo, Paloma Cejas, Prathapan Thiru, Martijn van de Bunt et al. The nuclear receptor THRB facilitates differentiation of human PSCs into more mature hepatocytes Cell Stem Cell 2022-05-01 [PMID: 35452598]
• Manuel Carrasco, Chencheng Wang, Anne M. Søviknes, Yngvild Bjørlykke, Shadab Abadpour, Joao A. Paulo et al. Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived beta -Like Cells Diabetes 2022-04-01 [PMID: 35043148]
• Wanjun Liu, James A. Flanders, Long‐Hai Wang, Qingsheng Liu, Daniel T. Bowers, Kai Wang et al. A Safe, Fibrosis‐Mitigating, and Scalable Encapsulation Device Supports Long‐Term Function of Insulin‐Producing Cells Small 2022-02-01 [PMID: 34897997]
• Minghui Wu, Michelle Y.Y. Lee, Varun Bahl, Daniel Traum, Jonathan Schug, Irina Kusmartseva et al. Single-cell analysis of the human pancreas in type 2 diabetes using multi-spectral imaging mass cytometry Cell Reports 2021-11-02 [PMID: 34731614]
• J Merkle, M Breunig, M Schmid, C Allgöwer, J Krüger, MK Melzer, S Bens, R Siebert, L Perkhofer, N Azoitei, T Seufferlei, S Heller, M Meier, M Müller, A Kleger, M Hohwieler CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome Cancers, 2021-10-13;13(20):. 2021-10-13 [PMID: 34680288] (Flow Cytometry, ICC, Human)
• Y Aghazadeh, F Poon, F Sarangi, FTM Wong, ST Khan, X Sun, R Hatkar, BJ Cox, SS Nunes, MC Nostro Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models Cell Stem Cell, 2021-09-03;0(0):. 2021-09-03 [PMID: 34480863] (Flow Cytometry, Human)
• NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines Nature Protocols, 2021-08-04;0(0):. 2021-08-04 [PMID: 34349281] (Flow Cytometry, ICC, Human)
• H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &amp;beta cells Nature Communications, 2021-06-07;12(1):3330. 2021-06-07 [PMID: 34099664] (IHC, Human)
• BSJ Low, CS Lim, SSL Ding, YS Tan, NHJ Ng, VG Krishnan, SF Ang, CWY Neo, CS Verma, S Hoon, SC Lim, ES Tai, AKK Teo Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant &amp;beta cells Nature Communications, 2021-05-25;12(1):3133. 2021-05-25 [PMID: 34035238] (ICC, Human)
• Elyne Backx, Elke Wauters, Jonathan Baldan, Mathias Van Bulck, Ellis Michiels, Yves Heremans et al. MECOM permits pancreatic acinar cell dedifferentiation avoiding cell death under stress conditions Cell Death & Differentiation 2021-09-01 [PMID: 33762742]
• Timothy M. Horton, Benjamin R. Kraemer, Justin P. Annes Protocol for determining zinc-dependent beta cell-selective small-molecule delivery in mouse pancreas STAR Protocols 2021-03-01 [PMID: 33490979]
• K Yang, M Lee, PA Jones, SS Liu, A Zhou, J Xu, V Sreekanth, JLY Wu, L Vo, EA Lee, R Pop, Y Lee, BK Wagner, DA Melton, A Choudhary, JM Karp A 3D culture platform enables development of zinc-binding prodrugs for targeted proliferation of &amp;beta cells Sci Adv, 2020-11-18;6(47):. 2020-11-18 [PMID: 33208361] (Flow Cytometry, Human)
• Bernard H, Teijeiro A, Chaves-Perez A et al. Coxsackievirus B Type 4 Infection in beta Cells Downregulates the Chaperone Prefoldin URI to Induce a MODY4-like Diabetes via Pdx1 Silencing Cell Reports Medicine 10/1/2020 [PMID: 33205075]
• Ma H, Jeppesen JF, Jaenisch R. Et al. Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive b Cell Destruction Cell Rep Med 11/18/2020 [PMID: 33205073]
  
  Details:
  Citation using the DyLight 488 version of this antibody.
• Reena Singh, Louise Cottle, Thomas Loudovaris, Di Xiao, Pengyi Yang, Helen E. Thomas et al. Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix Stem Cells Translational Medicine 2021-03-01 [PMID: 33145960]
• P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Single-Cell Transcriptome Profiling Reveals &amp;beta Cell Maturation in Stem Cell-Derived Islets after Transplantation Cell Rep, 2020-08-25;32(8):108067. 2020-08-25 [PMID: 32846125] (ICC, Human)
• R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells Cells, 2020-08-20;9(9):. 2020-08-20 [PMID: 32825270] (ICC, Human, Human`)
• L Velazco-Cr, MM Goedegebuu, KG Maxwell, P Augsornwor, NJ Hogrebe, JR Millman SIX2 Regulates Human &amp;beta Cell Differentiation from Stem Cells and Functional Maturation In�Vitro Cell Rep, 2020-05-26;31(8):107687. 5/26/2020 [PMID: 32460030]
• LSW Loo, AAP Soetedjo, HH Lau, NHJ Ng, S Ghosh, L Nguyen, VG Krishnan, H Choi, X Roca, S Hoon, AKK Teo BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells Cell Death Dis, 2020-05-18;11(5):378. 2020-05-18 [PMID: 32424151] (ICC, Human)
• Aharon Helman, Andrew L. Cangelosi, Jeffrey C. Davis, Quan Pham, Arielle Rothman, Aubrey L. Faust et al. A Nutrient-Sensing Transition at Birth Triggers Glucose-Responsive Insulin Secretion Cell Metabolism 2020-05-05 [PMID: 32375022]
• AK Blöchinger, J Siehler, K Wi beta miller, A Shahryari, I Burtscher, H Lickert Generation of an INSULIN-H2B-Cherry reporter human iPSC line Stem Cell Res, 2020-04-22;45(0):101797. 2020-04-22 [PMID: 32361463] (Flow Cytometry, Human)
• Mahaddalkar PU, Scheibner K, Pfluger S et al. Generation of pancreatic ? cells from CD177(+) anterior definitive endoderm Nature Biotechnology 2020-09-01 [PMID: 32341565]
• Seokho Kim, Robert L. Whitener, Heshan Peiris, Xueying Gu, Charles A. Chang, Jonathan Y. Lam et al. Molecular and genetic regulation of pig pancreatic islet cell development Development 2020-01-01 [PMID: 32108026]
• NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020-02-24;0(0):. 2020-02-24 [PMID: 32094658] (ICC, Human)
• Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020-02-17;21(3):274-286. 2020-02-17 [PMID: 32066947] (ICC, Human)
• JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton Circadian Entrainment Triggers Maturation of Human In�Vitro Islets Cell Stem Cell, 2019-12-12;26(1):108-122.e10. 2019-12-12 [PMID: 31839570] (ICC, Human)
• P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced J Pathol Clin Res, 2019-09-07;5(4):248-255. 2019-09-07 [PMID: 31493350] (IHC-P, Human)
• Punn Augsornworawat, Leonardo Velazco-Cruz, Jiwon Song, Jeffrey R. Millman A hydrogel platform for in vitro three dimensional assembly of human stem cell-derived islet cells and endothelial cells Acta Biomaterialia 2019-10-01 [PMID: 31446050]
• K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019-07-09;28(2):382-393.e7. 2019-07-09 [PMID: 31291575] (Flow Cytometry, ICC, Immunoprecipitation, Human)
• N Sharon, J Vanderhoof, J Straubhaar, J Mueller, R Chawla, Q Zhou, EN Engquist, C Trapnell, DK Gifford, DA Melton Wnt Signaling Separates the Progenitor and Endocrine Compartments during Pancreas Development Cell Rep, 2019-05-21;27(8):2281-2291.e5. 5/21/2019 [PMID: 31116975]
• EA Rosado-Oli, K Anderson, JH Kenty, DA Melton YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells Nat Commun, 2019-04-01;10(1):1464. 2019-04-01 [PMID: 30931946] (Flow Cytometry, IHC, Human)
• X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert Point mutations in the PDX1 transactivation domain impair human ?-cell development and function Mol Metab, 2019-03-20;0(0):. 2019-03-20 [PMID: 30930126] (Flow Cytometry, Human)
• Yue J. Wang, Daniel Traum, Jonathan Schug, Long Gao, Chengyang Liu, HPAP Consortium et al. Multiplexed In Situ Imaging Mass Cytometry Analysis of the Human Endocrine Pancreas and Immune System in Type 1 Diabetes Cell Metabolism 2019-03-01 [PMID: 30713110]
• Damond N, Engler S, Zanotelli VRT et al. A Map of Human Type 1 Diabetes Progression by Imaging Mass Cytometry Cell Metab. 1/11/2019 [PMID: 30713109]
• U Diekmann, H Wolling, R Dettmer, I Niwolik, O Naujok, FFR Buettner Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture Sci Rep, 2019-01-30;9(1):996. 2019-01-30 [PMID: 30700818] (Flow Cytometry, ICC, Human)
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  Details:
  This citation used the FITC version of this antibody.
• Sui L, Mfopou J, Geens M, Sermon K, Bouwens L FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells. Biochem Biophys Res Commun, 2012-08-29;426(3):380-5. 2012-08-29 [PMID: 22960178] (ICC, Human)
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• Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Stem Cells, 2011-04-01;29(4):609-17. 2011-04-01 [PMID: 21308865] (Flow Cytometry, Human)
• Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl. Acad. Sci. U.S.A., 2006-04-21;103(18):6907-12. 2006-04-21 [PMID: 16632596] (ICC, Human)

Bioinformatics

• Gene Symbol: PDX1
• Uniprot:
  • P52945()