PDX-1/IPF1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) ...read more
PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of ...read more
PDX‑1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-HumanPDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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PDX-1/IPF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Specificity
Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDX1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF2419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Unconjugated]

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDX-1/IPF1 Antibody (AF2419)(27)

We have publications tested in 3 confirmed species: Human, Rat, Human`.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation.


Filter By Application
Flow Cytometry
(6)
ICC
(16)
IHC
(2)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(25)
Rat
(1)
Human`
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF2419 Applications Species
P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Single-Cell Transcriptome Profiling Reveals &beta Cell Maturation in Stem Cell-Derived Islets after Transplantation Cell Rep, 2020;32(8):108067. 2020 [PMID: 32846125] (ICC, Human) ICC Human
R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells Cells, 2020;9(9):. 2020 [PMID: 32825270] (ICC, Human`) ICC Human`
LSW Loo, AAP Soetedjo, HH Lau, NHJ Ng, S Ghosh, L Nguyen, VG Krishnan, H Choi, X Roca, S Hoon, AKK Teo BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells Cell Death Dis, 2020;11(5):378. 2020 [PMID: 32424151] (ICC, Human) ICC Human
AK Blöchinger, J Siehler, K Wi beta miller, A Shahryari, I Burtscher, H Lickert Generation of an INSULIN-H2B-Cherry reporter human iPSC line Stem Cell Res, 2020;45(0):101797. 2020 [PMID: 32361463] (Flow Cytometry, Human) Flow Cytometry Human
NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020;0(0):. 2020 [PMID: 32094658] (ICC, Human) ICC Human
Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020;21(3):274-286. 2020 [PMID: 32066947] (ICC, Human) ICC Human
JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton Circadian Entrainment Triggers Maturation of Human In�Vitro Islets Cell Stem Cell, 2019;26(1):108-122.e10. 2019 [PMID: 31839570] (ICC, Human) ICC Human
P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced J Pathol Clin Res, 2019;5(4):248-255. 2019 [PMID: 31493350] (IHC-P, Human) IHC-P Human
K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019;28(2):382-393.e7. 2019 [PMID: 31291575] (Immunoprecipitation, Human) Immunoprecipitation Human
EA Rosado-Oli, K Anderson, JH Kenty, DA Melton YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells Nat Commun, 2019;10(1):1464. 2019 [PMID: 30931946] (Flow Cytometry, Human) Flow Cytometry Human
Show All 27 Publications.

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Biotin BAF2419

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