PDX-1/IPF1 Antibody [Unconjugated]

Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX‑1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

PDX‑1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-HumanPDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

PDX-1/IPF1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PDX‑1/IPF1
  Ala91-Arg283
  Accession # P52945
• Specificity: Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PDX1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Unconjugated]

• Glucose-sensitive factor
• IDX1
• IDX-1GSF
• Insulin promoter factor 1
• insulin promoter factor 1, homeodomain transcription factor
• Insulin upstream factor 1
• IPF1
• IPF1pancreas/duodenum homeobox protein 1
• Islet/duodenum homeobox-1
• MODY4IUF1
• pancreatic and duodenal homeobox 1
• pancreatic-duodenal homeobox factor 1
• PDX-1
• PDX-1IPF-1
• somatostatin transcription factor 1
• Somatostatin-transactivating factor 1
• STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDX-1/IPF1 Antibody (AF2419)(38)

Publications using AF2419

• FM Hermann, MF Kjærgaard, C Tian, U Tiemann, A Jackson, LR Olsen, M Kraft, PO Carlsson, IM Elfving, JLT Kettunen, T Tuomi, I Novak, H Semb An insulin hypersecretion phenotype precedes pancreatic beta cell failure in MODY3 patient-specific cells Cell Stem Cell, 2022;0(0):. 2022 [PMID: 36563694] (Flow Cytometry, Human)
• A Beucher, I Miguel-Esc, D Balboa, MG De Vas, MA Maestro, J Garcia-Hur, A Bernal, R Gonzalez-F, P Vargiu, H Heyn, P Ravassard, S Ortega, J Ferrer The HASTER lncRNA promoter is a cis-acting transcriptional stabilizer of HNF1A Nature Cell Biology, 2022;0(0):. 2022 [PMID: 36202974] (IHC, Human)
• EZ Sim, T Enomoto, N Shiraki, N Furuta, S Kashio, T Kambe, T Tsuyama, A Arakawa, H Ozawa, M Yokoyama, M Miura, S Kume Methionine metabolism regulates pluripotent stem cell pluripotency and differentiation through zinc mobilization Cell Reports, 2022;40(3):111120. 2022 [PMID: 35858556] (ICC, Human)
• JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew Divergent transcriptional regulation of astrocyte reactivity across disorders Nature, 2022;0(0):. 2022 [PMID: 35614216] (IHC, Mouse)
• J Merkle, M Breunig, M Schmid, C Allgöwer, J Krüger, MK Melzer, S Bens, R Siebert, L Perkhofer, N Azoitei, T Seufferlei, S Heller, M Meier, M Müller, A Kleger, M Hohwieler CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome Cancers, 2021;13(20):. 2021 [PMID: 34680288] (Flow Cytometry, Human)
• Y Aghazadeh, F Poon, F Sarangi, FTM Wong, ST Khan, X Sun, R Hatkar, BJ Cox, SS Nunes, MC Nostro Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34480863] (Flow Cytometry, Human)
• NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines Nature Protocols, 2021;0(0):. 2021 [PMID: 34349281] (Flow Cytometry, Human)
• H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells Nature Communications, 2021;12(1):3330. 2021 [PMID: 34099664] (IHC, Human)
• BSJ Low, CS Lim, SSL Ding, YS Tan, NHJ Ng, VG Krishnan, SF Ang, CWY Neo, CS Verma, S Hoon, SC Lim, ES Tai, AKK Teo Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant &beta cells Nature Communications, 2021;12(1):3133. 2021 [PMID: 34035238] (ICC, Human)
• K Yang, M Lee, PA Jones, SS Liu, A Zhou, J Xu, V Sreekanth, JLY Wu, L Vo, EA Lee, R Pop, Y Lee, BK Wagner, DA Melton, A Choudhary, JM Karp A 3D culture platform enables development of zinc-binding prodrugs for targeted proliferation of &beta cells Sci Adv, 2020;6(47):. 2020 [PMID: 33208361] (Flow Cytometry, Human)
• P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Single-Cell Transcriptome Profiling Reveals &beta Cell Maturation in Stem Cell-Derived Islets after Transplantation Cell Rep, 2020;32(8):108067. 2020 [PMID: 32846125] (ICC, Human)
• R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells Cells, 2020;9(9):. 2020 [PMID: 32825270] (ICC, Human)
• R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells Cells, 2020;9(9):. 2020 [PMID: 32825270] (ICC, Human`)
• LSW Loo, AAP Soetedjo, HH Lau, NHJ Ng, S Ghosh, L Nguyen, VG Krishnan, H Choi, X Roca, S Hoon, AKK Teo BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells Cell Death Dis, 2020;11(5):378. 2020 [PMID: 32424151] (ICC, Human)
• AK Blöchinger, J Siehler, K Wi beta miller, A Shahryari, I Burtscher, H Lickert Generation of an INSULIN-H2B-Cherry reporter human iPSC line Stem Cell Res, 2020;45(0):101797. 2020 [PMID: 32361463] (Flow Cytometry, Human)
• NJ Hogrebe, P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells Nat. Biotechnol., 2020;0(0):. 2020 [PMID: 32094658] (ICC, Human)
• Y Wang, L Lifshitz, K Gellatly, CL Vinton, K Busman-Sah, S McCauley, P Vangala, K Kim, A Derr, S Jaiswal, A Kucukural, P McDonel, PW Hunt, T Greenough, J Houghton, M Somsouk, JD Estes, JM Brenchley, M Garber, SG Deeks, J Luban HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells Nat. Immunol., 2020;21(3):274-286. 2020 [PMID: 32066947] (ICC, Human)
• JR Alvarez-Do, J Donaghey, N Rasouli, JHR Kenty, A Helman, J Charlton, JR Straubhaar, A Meissner, DA Melton Circadian Entrainment Triggers Maturation of Human In�Vitro Islets Cell Stem Cell, 2019;26(1):108-122.e10. 2019 [PMID: 31839570] (ICC, Human)
• P Seiron, A Wiberg, E Kuric, L Krogvold, FL Jahnsen, K Dahl-Jørge, O Skog, O Korsgren Characterisation of the endocrine pancreas in type 1 diabetes: islet size is maintained but islet number is markedly reduced J Pathol Clin Res, 2019;5(4):248-255. 2019 [PMID: 31493350] (IHC-P, Human)
• K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019;28(2):382-393.e7. 2019 [PMID: 31291575] (Immunoprecipitation, Human)
• EA Rosado-Oli, K Anderson, JH Kenty, DA Melton YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing ? cells Nat Commun, 2019;10(1):1464. 2019 [PMID: 30931946] (Flow Cytometry, Human)
• X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert Point mutations in the PDX1 transactivation domain impair human ?-cell development and function Mol Metab, 2019;0(0):. 2019 [PMID: 30930126] (Flow Cytometry, Human)
• U Diekmann, H Wolling, R Dettmer, I Niwolik, O Naujok, FFR Buettner Chemically defined and xenogeneic-free differentiation of human pluripotent stem cells into definitive endoderm in 3D culture Sci Rep, 2019;9(1):996. 2019 [PMID: 30700818] (Flow Cytometry, Human)
• T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival Nat Commun, 2018;9(1):4815. 2018 [PMID: 30446643] (ICC, Human)
• H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors Stem Cell Res Ther, 2018;9(1):277. 2018 [PMID: 30359326] (ICC, Human)
• R Wei, F Yuan, Y Wu, Y Liu, K You, Z Yang, Y Chen, A Getachew, N Wang, Y Xu, Y Zhuang, F Yang, YX Li Construction of a GLI3 compound heterozygous knockout human embryonic stem cell line WAe001-A-20 by CRISPR/Cas9 editing Stem Cell Res, 2018;32(0):139-144. 2018 [PMID: 30278376] (ICC, Human)
• PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity Dev. Cell, 2018;44(6):679-693.e5. 2018 [PMID: 29587142] (IHC-Fr, Human)
• T Robert, I De Mesmaek, GM Stangé, KG Suenens, Z Ling, EJ Kroon, DG Pipeleers Functional Beta Cell Mass from Device-Encapsulated hESC-Derived Pancreatic Endoderm Achieving Metabolic Control Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29503087] (ICC, Human)
• KF Cogger, A Sinha, F Sarangi, EC McGaugh, D Saunders, C Dorrell, S Mejia-Guer, Y Aghazadeh, JL Rourke, RA Screaton, M Grompe, PR Streeter, AC Powers, M Brissova, T Kislinger, MC Nostro Glycoprotein 2 is a specific cell surface marker of human pancreatic progenitors Nat Commun, 2017;8(1):331. 2017 [PMID: 28835709] (Flow Cytometry, Human)
• Y Hosokawa, T Toyoda, K Fukui, MY Baden, M Funato, Y Kondo, T Sudo, H Iwahashi, M Kishida, C Okada, A Watanabe, I Asaka, K Osafune, A Imagawa, I Shimomura Insulin-producing cells derived from 'induced pluripotent stem cells' of patients with fulminant type 1 diabetes: vulnerability to cytokine insults and increased expression of apoptosis-related genes J Diabetes Investig, 2017;0(0):. 2017 [PMID: 28796422] (ICC, Human)
• H Liu, D Guo, A Ruzi, Y Chen, T Pan, F Yang, J Li, K Xu, T Zhou, D Qin, YX Li Testosterone improves the differentiation efficiency of insulin-producing cells from human induced pluripotent stem cells PLoS ONE, 2017;12(6):e0179353. 2017 [PMID: 28594910] (ICC, Human)
• J Saarimäki-, D Balboa, MA Russell, J Saarikettu, M Kinnunen, S Keskitalo, A Malhi, C Valensisi, C Andrus, S Eurola, H Grym, J Ustinov, K Wartiovaar, RD Hawkins, O Silvennoin, M Varjosalo, NG Morgan, T Otonkoski An Activating STAT3 Mutation Causes Neonatal Diabetes through Premature Induction of Pancreatic Differentiation Cell Rep, 2017;19(2):281-294. 2017 [PMID: 28402852] (ICC, Human)
• Claudia Davenport The Anterior-Posterior Patterning of Definitive Endoderm Generated from Human Embryonic Stem Cells Depends on the Differential Signaling of Retinoic Acid, Wnt- and BMP-Signaling Stem Cells, 2016;0(0):. 2016 [PMID: 27299363] (IHC, Human)
• Zhao Z, Low Y, Armstrong N, Ryu J, Sun S, Arvanites A, Hollister-Lock J, Shah N, Weir G, Annes J Repurposing cAMP-modulating medications to promote beta-cell replication. Mol Endocrinol, 2014;28(10):1682-97. 2014 [PMID: 25083741] (IHC, Rat)
• Sui L, Mfopou J, Geens M, Sermon K, Bouwens L FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells. Biochem Biophys Res Commun, 2012;426(3):380-5. 2012 [PMID: 22960178] (ICC, Human)
• Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 2012;21(6):1321-39. 2012 [PMID: 22195604] (ICC, Human)
• Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Stem Cells, 2011;29(4):609-17. 2011 [PMID: 21308865] (Flow Cytometry, Human)
• Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl. Acad. Sci. U.S.A., 2006;103(18):6907-12. 2006 [PMID: 16632596] (ICC, Human)

Bioinformatics

• Gene Symbol: PDX1
• Uniprot:
  • P52945()