PDX-1/IPF1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) ...read more
PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Goat Anti-Human PDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX‑1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of ...read more
PDX‑1/IPF1 was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Goat Anti-HumanPDX‑1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2419) at 15 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

PDX-1/IPF1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Specificity
Detects human PDX‑1/IPF1 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse PDX-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDX1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Unconjugated]

  • Glucose-sensitive factor
  • IDX1
  • IDX-1GSF
  • Insulin promoter factor 1
  • insulin promoter factor 1, homeodomain transcription factor
  • Insulin upstream factor 1
  • IPF1
  • IPF1pancreas/duodenum homeobox protein 1
  • Islet/duodenum homeobox-1
  • MODY4IUF1
  • pancreatic and duodenal homeobox 1
  • pancreatic-duodenal homeobox factor 1
  • PDX-1
  • PDX-1IPF-1
  • somatostatin transcription factor 1
  • Somatostatin-transactivating factor 1
  • STF-1IUF-1

Background

PDX-1, also known as islet/duodenum homeobox-1 (IDX-1), is a homeodomain-containing transcription factor. During embryonic development, PDX-1 is required for pancreas differentiation in adult islet cells. PDX-1 regulates beta -cell specific gene expression and function. Human and mouse PDX-1 share 88% amino acid sequence homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDX-1/IPF1 Antibody (AF2419)(35)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Human`.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation.


Filter By Application
Flow Cytometry
(10)
ICC
(18)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
All Applications
Filter By Species
Human
(32)
Mouse
(1)
Rat
(1)
Human`
(1)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using AF2419 Applications Species
JE Burda, TM O'Shea, Y Ao, KB Suresh, S Wang, AM Bernstein, A Chandra, S Deverasett, R Kawaguchi, JH Kim, S McCallum, A Rogers, S Wahane, MV Sofroniew Divergent transcriptional regulation of astrocyte reactivity across disorders Nature, 2022;0(0):. 2022 [PMID: 35614216] (IHC, Mouse) IHC Mouse
J Merkle, M Breunig, M Schmid, C Allgöwer, J Krüger, MK Melzer, S Bens, R Siebert, L Perkhofer, N Azoitei, T Seufferlei, S Heller, M Meier, M Müller, A Kleger, M Hohwieler CDKN2A-Mutated Pancreatic Ductal Organoids from Induced Pluripotent Stem Cells to Model a Cancer Predisposition Syndrome Cancers, 2021;13(20):. 2021 [PMID: 34680288] (Flow Cytometry, Human) Flow Cytometry Human
Y Aghazadeh, F Poon, F Sarangi, FTM Wong, ST Khan, X Sun, R Hatkar, BJ Cox, SS Nunes, MC Nostro Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34480863] (Flow Cytometry, Human) Flow Cytometry Human
NJ Hogrebe, KG Maxwell, P Augsornwor, JR Millman Generation of insulin-producing pancreatic beta cells from multiple human stem cell lines Nature Protocols, 2021;0(0):. 2021 [PMID: 34349281] (Flow Cytometry, Human) Flow Cytometry Human
H Liu, R Li, HK Liao, Z Min, C Wang, Y Yu, L Shi, J Dan, A Hayek, L Martinez M, E Nuñez Deli, JC Izpisua Be Chemical combinations potentiate human pluripotent stem cell-derived 3D pancreatic progenitor clusters toward functional &beta cells Nature Communications, 2021;12(1):3330. 2021 [PMID: 34099664] (IHC, Human) IHC Human
BSJ Low, CS Lim, SSL Ding, YS Tan, NHJ Ng, VG Krishnan, SF Ang, CWY Neo, CS Verma, S Hoon, SC Lim, ES Tai, AKK Teo Decreased GLUT2 and glucose uptake contribute to insulin secretion defects in MODY3/HNF1A hiPSC-derived mutant &beta cells Nature Communications, 2021;12(1):3133. 2021 [PMID: 34035238] (ICC, Human) ICC Human
K Yang, M Lee, PA Jones, SS Liu, A Zhou, J Xu, V Sreekanth, JLY Wu, L Vo, EA Lee, R Pop, Y Lee, BK Wagner, DA Melton, A Choudhary, JM Karp A 3D culture platform enables development of zinc-binding prodrugs for targeted proliferation of &beta cells Sci Adv, 2020;6(47):. 2020 [PMID: 33208361] (Flow Cytometry, Human) Flow Cytometry Human
P Augsornwor, KG Maxwell, L Velazco-Cr, JR Millman Single-Cell Transcriptome Profiling Reveals &beta Cell Maturation in Stem Cell-Derived Islets after Transplantation Cell Rep, 2020;32(8):108067. 2020 [PMID: 32846125] (ICC, Human) ICC Human
R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells Cells, 2020;9(9):. 2020 [PMID: 32825270] (ICC, Human) ICC Human
R Dettmer, K Cirksena, J Münchhoff, J Kresse, U Diekmann, I Niwolik, FFR Buettner, O Naujok FGF2 Inhibits Early Pancreatic Lineage Specification during Differentiation of Human Embryonic Stem Cells Cells, 2020;9(9):. 2020 [PMID: 32825270] (ICC, Human`) ICC Human`
Show All 35 Publications.

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