GATA-4 Antibody

Images

 
Western Blot: GATA-4 Antibody [NBP2-24585] - Analysis of GATA-4 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and ...read more
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Formalin-fixed, paraffin-embedded human brain (cortex) probed with GATA-4 antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Formalin-fixed, paraffin-embedded human prostate probed with GATA-4 antibody at 10 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

GATA-4 Antibody Summary

Immunogen
A portion of amino acids 330-380 of human GATA-4 was used as the immunogen for the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GATA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Positive Control
GATA-4 Lysate (NBP2-66336)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24585 in the following applications:

Reactivity Notes

The amino acid sequence used as immunogen for the HSP27 antibody is 100% homologous in human, horse, macaque, chimpanzee and dog, 90% homologous in opossum, cow, zebrafish, boar and Xenopus and 81% homologous in rat and mouse.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.2 ml PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GATA-4 Antibody

  • GATA binding protein 4
  • GATA4
  • GATA-4
  • GATA-binding factor 4
  • GATA-binding protein 4
  • MGC126629
  • transcription factor GATA-4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-4 Antibody (NBP2-24585) (0)

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Review for GATA-4 Antibody (NBP2-24585) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24585:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GATA-4 NBP2-24585
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reviewed by:
Anonymous
IHC-P Human 12/02/2016
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpancreatic tissue
SpeciesHuman
Lot07213717C

Comments

CommentsPermeabilization with 0.1% triton was performed prior to heat mediated low pH antigen retrieval. The antibody was used at a dilution of 1/50 for 1 hr RT. An alexa fluor anti-rabbit 488 was used to detect the primary antibody.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GATA-4 Antibody (NBP2-24585) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
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Anonymous
12/02/2016
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol GATA4
Entrez
Uniprot