GATA-4 Antibody - BSA Free

Images

 
Western Blot: GATA-4 Antibody [NBP2-24585] - Analysis of GATA-4 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and ...read more
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Analysis of a FFPE tissue section of human kidney using 1:200 dilution of GATA-4 antibody (NBP2-24585). The staining was developed using HRP labeled ...read more
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Formalin-fixed, paraffin-embedded human prostate probed with GATA-4 antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Formalin-fixed, paraffin-embedded human brain (cortex) probed with GATA-4 antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: GATA-4 Antibody [NBP2-24585] - Staining in human pancreatic tissue stained for GATA4 (green), nuclear localization.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

GATA-4 Antibody - BSA Free Summary

Immunogen
A portion of amino acids 330-380 of human GATA-4 was used as the immunogen for the antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GATA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml. Use reported by customer review
  • Western Blot 1-3 ug/ml
Reviewed Applications
Read 1 Review rated 5
using
NBP2-24585 in the following applications:

Publications
Read Publications using
NBP2-24585 in the following applications:

  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28132888).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GATA-4 Antibody - BSA Free

  • GATA binding protein 4
  • GATA4
  • GATA-4
  • GATA-binding factor 4
  • GATA-binding protein 4
  • MGC126629
  • transcription factor GATA-4

Background

GATA4 is a 46 kDa member of the GATA family of zinc finger containing transcription factors that is involved in the development of cardiac hypertrophy and remodeling, and plays a critical role in regulating basal and agonist or stress induced gene expression in cardiac and smooth muscle cell types. GATA4 contains a conserved MAPK phosphorylation site at serine 105 within the transcriptional activation domain. Serine 105 of GATA4 is phosphorylated in response to agonist stimulation through MEK 1 ERK1 / 2, and weakly through JNK or p38 MAPKs.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GATA-4 Antibody (NBP2-24585)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(2)
Mouse
(1)
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Showing Publications 1 - 2 of 2.
Publications using NBP2-24585 Applications Species
Qu Y, Cui X, Sareen D et al. In vitro induction of mammary-like differentiation from human pluripotent stem cells Patent 2018-08-02 (WB, Human) WB Human
Qu Y, Han B, Gao B et al. Differentiation of Human Induced Pluripotent Stem Cells to Mammarylike Organoids Stem Cell Reports. 2017-02-14 [PMID: 28132888] (WB, Mouse, Human) WB Mouse, Human

Review for GATA-4 Antibody (NBP2-24585) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24585:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin GATA-4 NBP2-24585
Enlarge
5
reviewed by:
Verified Customer
IHC-P Human 12/02/2016
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedpancreatic tissue
SpeciesHuman
Lot07213717C

Comments

CommentsPermeabilization with 0.1% triton was performed prior to heat mediated low pH antigen retrieval. The antibody was used at a dilution of 1/50 for 1 hr RT. An alexa fluor anti-rabbit 488 was used to detect the primary antibody.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GATA-4 Antibody (NBP2-24585) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
12/02/2016
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol GATA4
Uniprot