HNF-3 beta/FoxA2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL ...read more
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 ...read more
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
HNF‑3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

HNF-3 beta/FoxA2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HNF‑3 beta /FoxA2
Met242-Ser457
Accession # Q9Y261
Specificity
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXA2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF2400 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 beta/FoxA2 Antibody [Unconjugated]

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • HNF3BTCF-3B
  • MGC19807
  • TCF3B
  • Transcription factor 3B

Background

HNF-3 beta , also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-3 beta/FoxA2 Antibody (AF2400)(50)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate - Rhesus macaque.

We have publications tested in 8 applications: CyTof, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
CyTof
(1)
Flow Cytometry
(6)
ICC
(34)
IHC
(4)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(43)
Mouse
(5)
Rat
(1)
Primate - Rhesus macaque
(1)
All Species
Showing Publications 1 - 10 of 50. Show All 50 Publications.
Publications using AF2400 Applications Species
T Bluhmki, S Traub, AK Müller, S Bitzer, E Schruf, MT Bammert, M Leist, F Gantner, JP Garnett, R Heilker Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96?Transwell air-liquid interface model Scientific Reports, 2021;11(1):17028. 2021 [PMID: 34426605] (IHC, Human) IHC Human
A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels Cell Death & Disease, 2021;12(7):637. 2021 [PMID: 34155199] (IHC, Human) IHC Human
S Chen, Y Han, L Yang, T Kim, M Nair, O Harschnitz, P Wang, J Zhu, SY Koo, X Tang, L Lacko, V Chandar, Y Bram, T Zhang, W Zhang, F He, J Caicedo, Y Huang, T Evans, P van der Va, MJ Titulaer, JKH Spoor, RL Furler, P Canoll, J Goldman, S Przedborsk, R Schwartz, D Ho, L Studer SARS-CoV-2 Infection Causes Dopaminergic Neuron Senescence Research square, 2021;0(0):. 2021 [PMID: 34031650] (IHC, Human) IHC Human
H Mou, Y Yang, MA Riehs, J Barrios, M Shivaraju, AL Haber, DT Montoro, K Gilmore, EA Haas, B Paunovic, J Rajagopal, SO Vargas, RL Haynes, A Fine, WV Cardoso, X Ai Airway basal stem cells generate distinct subpopulations of PNECs Cell Reports, 2021;35(3):109011. 2021 [PMID: 33882306] (ICC, Human) ICC Human
Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys Nature Medicine, 2021;0(0):. 2021 [PMID: 33649496] (ICC, Primate - Rhesus macaque) ICC Primate - Rhesus macaque
J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01 Cell Stem Cell, 2021;28(2):217-229.e7. 2021 [PMID: 33545080] (Flow Cytometry, Human) Flow Cytometry Human
B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, R Corominas, I Cuscó Generation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering Williams-Beuren syndrome (7q11.23 deletion) Stem Cell Research, 2020;49(0):102087. 2020 [PMID: 33370870] (Flow Cytometry, Human) Flow Cytometry Human
B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, I Cuscó, R Corominas Derivation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering 7q11.23 microduplication syndrome Stem Cell Res, 2020;49(0):102092. 2020 [PMID: 33254092] (ICC, Human) ICC Human
M Inglés-Fer, M Martin-Ina, L Herrera, M Villaverde, S Santos, MA Vesga, E Garreta, I Martín-Rui, AM Aransay, J Anguita, B Barreña, LM Allende, LI Gonzalez-G, C Eguizabal Generation, establishment and characterization of a pluripotent stem cell line (CVTTHi001-A) from primary fibroblasts isolated from a patient with activated PI3 kinase delta syndrome (APDS2) Stem Cell Res, 2020;49(0):102082. 2020 [PMID: 33221676] (ICC, Human) ICC Human
E Naumovska, G Aalderink, C Wong Valen, K Kosim, A Nicolas, S Brown, P Vulto, KS Erdmann, D Kurek Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells Int J Mol Sci, 2020;21(14):. 2020 [PMID: 32674311] (ICC, Human) ICC Human
Show All 50 Publications.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 IC2400G
Biotin BAF2400

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Bioinformatics

Gene Symbol FOXA2
Uniprot