HNF-3 beta/FoxA2 Antibody [Unconjugated]


Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF-3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human HNF-3 beta /FoxA2 more
HNF-3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
HNF-3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL for 1 hour more
HNF‑3 beta /FoxA2 was detected in immersion fixed HepG2 Human Hepatocellular Carcinoma Cells (Positive) and absent in THP‑1 Human Acute Monocytic Leukemia Cells (Negative) using Goat Anti-Human more
Western blot shows lysates of HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
Differentiation of hESCs into definitive endoderm cells by Activin A with CHIR99021.a The relative primitive streak (BRACHYRUY, MESP1, and MIXL1) gene expression of the day 1 differentiated cells by adding Activin A more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF

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HNF-3 beta/FoxA2 Antibody [Unconjugated] Summary

E. coli-derived recombinant human HNF‑3 beta /FoxA2
Accession # Q9Y261
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF2400 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 beta/FoxA2 Antibody [Unconjugated]

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • MGC19807
  • TCF3B
  • Transcription factor 3B


HNF-3 beta , also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-3 beta/FoxA2 Antibody (AF2400)(95)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Primate - Rhesus macaque, Transgenic Rat, Xenograft.

We have publications tested in 9 applications: ChIP, CyTof, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.

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Flow Cytometry
Western Blot
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Primate - Rhesus macaque
Transgenic Rat
All Species
Showing Publications 1 - 10 of 95. Show All 95 Publications.
Publications using AF2400 Applications Species
Maimaitili, M;Chen, M;Febbraro, F;Ucuncu, E;Kelly, R;Niclis, JC;Christiansen, JR;Mermet-Joret, N;Niculescu, D;Lauritsen, J;Iannielli, A;Klæstrup, IH;Jensen, UB;Qvist, P;Nabavi, S;Broccoli, V;Nykjær, A;Romero-Ramos, M;Denham, M; Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells Nature communications 2023-12-05 [PMID: 38052784] (ICC, IHC, Xenograft, Human) ICC, IHC Xenograft, Human
Pavlinov, I;Tambe, M;Abbott, J;Nguyen, HN;Xu, M;Pradhan, M;Farkhondeh, A;Zheng, W; In depth characterization of midbrain organoids derived from wild type iPSC lines PloS one 2023-10-20 [PMID: 37862312] (Human) Human
Boeing, A;Mavrommatis, L;Daya, NM;Zhuge, H;Volke, L;Kocabas, A;Kneifel, M;Athamneh, M;Krause, K;Südkamp, N;Döring, K;Theiss, C;Roos, A;Zaehres, H;Güttsches, AK;Vorgerd, M; Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease Stem cell research 2023-10-04 [PMID: 37839261] (ICC, Human) ICC Human
Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F; Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative Stem cell research 2023-09-21 [PMID: 37748331] (ICC, Human) ICC Human
Souralova, T;Hulinova, D;Jeseta, M;Ventruba, P;Hampl, A;Koutna, I; Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells Scientific reports 2023-09-12 [PMID: 37700192] (ICC, Human) ICC Human
Montero-Calle, P;Flandes-Iparraguirre, M;Kuebler, B;Arán, B;Larequi, E;Anaut, I;Coppiello, G;Aranguren, XL;Veiga, A;Elorz, MTB;de Yébenes, MG;Gavira, JJ;Prósper, F;Iglesias-García, O;Vega, MMM; Generation of an induced pluripotent stem cell line (ESi107-A) from a transthyretin amyloid cardiomyopathy (ATTR-CM) patient carrying a p.Ser43Asn mutation in the TTR gene Stem cell research 2023-09-01 [PMID: 37660554] (ICC, Human) ICC Human
Lin Feng, Da Li, Yao Tian, Chengshun Zhao, Yun Sun, Xiaolong Kou et al. One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo Neural Regeneration Research 2024-02-01 [PMID: 37488911]
Muwan Chen, Jonathan Christos Niclis, Mark Denham Protocol for generating reproducible miniaturized controlled midbrain organoids STAR Protocols 2023-09-01 [PMID: 37481727]
Takeshi Kato, Kaneyasu Nishimura, Masahiro Hirao, Shun Shimohama, Kazuyuki Takata Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells Neuropsychopharmacology Reports 2023-06-27 [PMID: 37366076]
Kaneyasu Nishimura, Emilía Sif Ásgrímsdóttir, Shanzheng Yang, Ernest Arenas A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons STAR Protocols 2023-09-01 [PMID: 37310863]
Show All 95 Publications.

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Gene Symbol FOXA2