HNF-3 beta/FoxA2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL ...read more
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

HNF-3 beta/FoxA2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HNF‑3 beta /FoxA2
Met242-Ser457
Accession # Q9Y261
Specificity
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXA2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publications using
AF2400 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 beta/FoxA2 Antibody [Unconjugated]

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • HNF3BTCF-3B
  • MGC19807
  • TCF3B
  • Transcription factor 3B

Background

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-3 beta/FoxA2 Antibody (AF2400)(27)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: CyTof, Flow, ICC, ICC Fluorescence, IHC Fresh, IHC Paraffin-embedded, IHC-Fluorescent, WB.


Filter By Application
CyTof
(1)
Flow
(4)
ICC
(17)
ICC Fluorescence
(1)
IHC Fresh
(1)
IHC Paraffin-embedded
(1)
IHC-Fluorescent
(1)
WB
(1)
All Applications
Filter By Species
Human
(23)
Mouse
(3)
Rat
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF2400 Applications Species
B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS) Stem Cell Res, 2017;0(0):. 2017 [PMID: 29150092] (ICC,, Human) ICC Human
L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines Stem Cell Res, 2017;24(0):155-159. 2017 [PMID: 29034884] (ICC,, Human) ICC Human
KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28602613] (ICC,, Human) ICC Human
JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency Cell Rep, 2017;19(1):20-35. 2017 [PMID: 28380358] (Flow,, Human) Flow Human
F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells Nat Protoc, 2017;12(4):814-827. 2017 [PMID: 28333915] (Flow,, Human) Flow Human
A Shiraishi, K Muguruma, Y Sasai Generation of thalamic neurons from mouse embryonic stem cells Development, 2017;0(0):. 2017 [PMID: 28219951] (ICC,, Mouse) ICC Mouse
Cell Expansion During Directed Differentiation Of Stem Cells Toward The Hepatic Lineage Stem Cells Dev., 2016;0(0):. 2016 [PMID: 27806669] (CyTof,, Human) CyTof Human
Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells Sci Rep, 2016;6(0):31063. 2016 [PMID: 27507707] (IHC-Fluorescent,, Mouse) IHC-Fluorescent Mouse
Tsankov A, Akopian V, Pop R, Chetty S, Gifford C, Daheron L, Tsankova N, Meissner A A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells. Nat Biotechnol, 2015;33(11):1182-92. 2015 [PMID: 26501952] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Villa-Diaz L, Kim J, Lahann J, Krebsbach P Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates. Stem Cells Transl Med, 2014;3(12):1410-7. 2014 [PMID: 25313201] (ICC,, Human) ICC Human
Show All 27 Publications.

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Bioinformatics

Gene Symbol FOXA2
Entrez
Uniprot