HNF-3 beta/FoxA2 Antibody [Unconjugated]


Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL more
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 more
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
HNF‑3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF

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HNF-3 beta/FoxA2 Antibody [Unconjugated] Summary

E. coli-derived recombinant human HNF‑3 beta /FoxA2
Accession # Q9Y261
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
HNF-3 beta/FoxA2 Knockout HeLa Cell Lysate
Read Publications using
AF2400 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 beta/FoxA2 Antibody [Unconjugated]

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • MGC19807
  • TCF3B
  • Transcription factor 3B


HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-3 beta/FoxA2 Antibody (AF2400)(39)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: CyTof, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.

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Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using AF2400 Applications Species
Z Yang, X Jiang, Z Zhang, Z Zhao, W Xing, Y Liu, X Jiang, H Zhao HDAC3-dependent transcriptional repression of FOXA2 regulates FTO/m6A/MYC signaling to contribute to the development of gastric cancer Cancer Gene Ther., 2020;0(0):. 2020 [PMID: 32655129] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 2020;10(1):5989. 2020 [PMID: 32249832] (ICC, Human) ICC Human
YY Kim, H Kim, CS Suh, HC Liu, Z Rosenwaks, SY Ku Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model Int J Mol Sci, 2020;21(3):. 2020 [PMID: 31991577] (ICC, Human) ICC Human
N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency Stem Cell Reports, 2019;13(6):1083-1098. 2019 [PMID: 31708477] (ICC, Human) ICC Human
M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons Cell Stem Cell, 2019;25(4):514-530.e8. 2019 [PMID: 31543366] (ICC, Human) ICC Human
K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019;28(2):382-393.e7. 2019 [PMID: 31291575] (Western Blot, Human) Western Blot Human
C Calatayud, G Carola, I Fernández-, M Valtorta, S Jiménez-De, M Díaz, J Soriano-Fr, G Cappellett, J García-San, Á Raya, A Consiglio CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons Sci Rep, 2019;9(1):6811. 2019 [PMID: 31048719] (ICC, Human) ICC Human
T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival Nat Commun, 2018;9(1):4815. 2018 [PMID: 30446643] (ICC, Human) ICC Human
H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors Stem Cell Res Ther, 2018;9(1):277. 2018 [PMID: 30359326] (ICC, Human) ICC Human
E Senís, L Mosteiro, S Wilkening, E Wiedtke, A Nowrouzi, S Afzal, R Fronza, H Landerer, M Abad, D Niopek, M Schmidt, M Serrano, D Grimm AAVvector-mediated in vivo reprogramming into pluripotency Nat Commun, 2018;9(1):2651. 2018 [PMID: 29985406] (ICC, Mouse) ICC Mouse
Show All 39 Publications.

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 488 IC2400G
Biotin BAF2400

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Gene Symbol FOXA2