HNF-3 beta/FoxA2 Antibody [Unconjugated]


Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL more
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 more
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # more
HNF‑3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL more

Product Details

Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF

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HNF-3 beta/FoxA2 Antibody [Unconjugated] Summary

E. coli-derived recombinant human HNF‑3 beta /FoxA2
Accession # Q9Y261
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF2400 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 beta/FoxA2 Antibody [Unconjugated]

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • MGC19807
  • TCF3B
  • Transcription factor 3B


HNF-3 beta , also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-3 beta/FoxA2 Antibody (AF2400)(56)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate - Rhesus macaque.

We have publications tested in 9 applications: ChIP, CyTof, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.

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Flow Cytometry
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Primate - Rhesus macaque
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Showing Publications 1 - 10 of 56. Show All 56 Publications.
Publications using AF2400 Applications Species
T Tada, H Ohnishi, N Yamamoto, F Kuwata, Y Hayashi, H Okuyama, T Morino, Y Kasai, H Kojima, K Omori Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats Regenerative Therapy, 2022;19(0):77-87. 2022 [PMID: 35097166] (IHC, Rat) IHC Rat
P Aldrin-Kir, M Åkerblom, T Cardoso, S Nolbrant, AF Adler, X Liu, A Heuer, M Davidsson, M Parmar, T Björklund A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants Stem Cell Reports, 2021;17(1):159-172. 2021 [PMID: 34971563] (ICC, Human) ICC Human
RJ Geusz, A Wang, DK Lam, NK Vinckier, KD Alysandrat, DA Roberts, J Wang, S Kefalopoul, A Ramirez, Y Qiu, J Chiou, KJ Gaulton, B Ren, DN Kotton, M Sander Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors Nature Communications, 2021;12(1):6636. 2021 [PMID: 34789735] (ChIP, Human) ChIP Human
T Bluhmki, S Traub, AK Müller, S Bitzer, E Schruf, MT Bammert, M Leist, F Gantner, JP Garnett, R Heilker Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96?Transwell air-liquid interface model Scientific Reports, 2021;11(1):17028. 2021 [PMID: 34426605] (IHC, Human) IHC Human
A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels Cell Death & Disease, 2021;12(7):637. 2021 [PMID: 34155199] (IHC, Human) IHC Human
S Chen, Y Han, L Yang, T Kim, M Nair, O Harschnitz, P Wang, J Zhu, SY Koo, X Tang, L Lacko, V Chandar, Y Bram, T Zhang, W Zhang, F He, J Caicedo, Y Huang, T Evans, P van der Va, MJ Titulaer, JKH Spoor, RL Furler, P Canoll, J Goldman, S Przedborsk, R Schwartz, D Ho, L Studer SARS-CoV-2 Infection Causes Dopaminergic Neuron Senescence Research square, 2021;0(0):. 2021 [PMID: 34031650] (IHC, Human) IHC Human
H Mou, Y Yang, MA Riehs, J Barrios, M Shivaraju, AL Haber, DT Montoro, K Gilmore, EA Haas, B Paunovic, J Rajagopal, SO Vargas, RL Haynes, A Fine, WV Cardoso, X Ai Airway basal stem cells generate distinct subpopulations of PNECs Cell Reports, 2021;35(3):109011. 2021 [PMID: 33882306] (ICC, Human) ICC Human
G Guo, GG Stirparo, SE Strawbridg, D Spindlow, J Yang, J Clarke, A Dattani, A Yanagida, MA Li, S Myers, BN Özel, J Nichols, A Smith Human naive epiblast cells possess unrestricted lineage potential Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33831366] (ICC, Human) ICC Human
Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys Nature Medicine, 2021;0(0):. 2021 [PMID: 33649496] (ICC, Primate - Rhesus macaque) ICC Primate - Rhesus macaque
TW Kim, J Piao, SY Koo, S Kriks, SY Chung, D Betel, ND Socci, SJ Choi, S Zabierowsk, BN Dubose, EJ Hill, EV Mosharov, S Irion, MJ Tomishima, V Tabar, L Studer Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use Cell Stem Cell, 2021;28(2):343-355.e5. 2021 [PMID: 33545081] (ICC, Human) ICC Human
Show All 56 Publications.

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Gene Symbol FOXA2