HNF-3 beta/FoxA2 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HNF‑3 beta /FoxA2 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF-3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) or control antibody (AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (MAG999) and DNA was purified using chelating resin solution. TheE-RABPpromoter was detected by standard PCR.

HNF-3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

HNF-3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF-3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Left: Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 1 mg/ml. A specific band was detected for HNF‑3 beta /FoxA2 at approximately 61 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the HepG2 human hepatocellular carcinoma cell line.

HNF‑3 beta /FoxA2 was detected in immersion fixed HepG2 Human Hepatocellular Carcinoma Cells (Positive) and absent in THP‑1 Human Acute Monocytic Leukemia Cells (Negative) using Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Differentiation of hESCs into definitive endoderm cells by Activin A with CHIR99021.a The relative primitive streak (BRACHYRUY, MESP1, and MIXL1) gene expression of the day 1 differentiated cells by adding Activin A (100 ng/mL) with CHIR99021 (2–5 µM) or Wnt3a (25–100 ng/mL) treatments were determined by real-time quantitative PCR (qPCR). None, no WNT signaling pathway activators were used on day 1 for PS differentiation (Activin A only). b, c After 1 day, the medium was changed to Activin A (100 ng/mL) and 1 × CTS-B27 to induce DE differentiation. The definitive endoderm (FOXA2 and SOX17) and mesoderm (MESP2 and HAND1) relative gene expression levels were determined by qPCR. d Immunofluorescence analysis of the expression of SOX17 and FoxA2 for Activin A with 2 µM CHIR99021-induced differentiated cells on day 3. e, f The expression of SOX17 and FoxA2 for Activin A with 2 µM CHIR99021-induced differentiated cells was determined by flow cytometry on day 3. Isotype control antibodies were used as controls. At a specific gene expression, datum points lacking common letters differ, p human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue).

FOXA2 drives the p3-specific chromatin accessibility program(A) Footprinting score for the FOX motif is highest in p3 samples.(B) Foxa2 expression in p3 NPs suggests it is the most likely candidate to drive the footprinting signal.(C) Average ATAC-seq accessibility at FOXA2 ChIP-seq peaks13 in the indicated samples shows these regions are highly accessible in p3 NPs.(D) Normalized FOXA2 ChIP-seq coverage showing accessibility in p3-specific elements from the groups of ATAC-seq elements in the indicated clusters from Figure 3B.(E) Genetic lineage tracing indicates that cells that expressed Foxa2 at E8.5 (tamoxifen administration) have contributed to the p3 progenitor and V3 neuronal cell types by E11.5 (red arrows).(F) Quantifications of p3 and V3 cells expressing tdTomato in embryos collected after tamoxifen administration at the indicated times. Biological replicates: n = 2 for E7.5, n = 4 for E8.5, n = 3 for E9.5.(G) Foxa2−/− ES cells fail to generate p3 NPs when exposed to 500 nM SAG.(H) Representative flow cytometry plots of the quantifications in (G) showing a marked reduction in p3 NPs from Foxa2−/− ES cells compared with wild type. Cells are gated for SOX2+ live neural progenitors.(I) Representative immunohistochemistry staining for SOX2, OLIG2 and NKX2,2 showing reduced number of cells expressing NKX2.2 in Foxa2−/− mutant cells at day 6 of differentiation treated with 500 nM SAG.Scale bars, 50 μm.See also Figure S4. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36516856), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression profiles and functional enrichments of marker genes across time points identified during hESC differentiation. A. UMAP visualization of the expression of well-defined marker genes of different stages in hESC differentiation. B. The representative immunofluorescence staining for definitive endoderm cells with antibodies against SOX17 and FOXA2. DAPI serves as a nucleus indicator. The individual color channels were merged to assess the colocalization of SOX17 an FOXA2 expression in the nuclei. Scale bars, 200 μm. C. Dot plot showing the average and percentage expression of well-defined marker genes in different stages or cell types. Genes are colored according to their mean expression level. Diameter denotes fractional expression. SC-alpha , SC-beta , and SC-EC represent stem-cell-derived alpha , beta , and enterochromaffin cells, respectively. D. Heatmap showing the expression of the top ten marker genes of each time point. lncRNA genes are indicated by arrows. E. Enriched GO terms of marker genes of each time point. F. UMAP visualization of the expression of genes related to endoderm formation. G. Violin plots showing the expression of lncRNA genes that listed in the top 10 marker genes of each time point Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36907881), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression profiles and functional enrichments of marker genes across time points identified during hESC differentiation. A. UMAP visualization of the expression of well-defined marker genes of different stages in hESC differentiation. B. The representative immunofluorescence staining for definitive endoderm cells with antibodies against SOX17 and FOXA2. DAPI serves as a nucleus indicator. The individual color channels were merged to assess the colocalization of SOX17 an FOXA2 expression in the nuclei. Scale bars, 200 μm. C. Dot plot showing the average and percentage expression of well-defined marker genes in different stages or cell types. Genes are colored according to their mean expression level. Diameter denotes fractional expression. SC-alpha , SC-beta , and SC-EC represent stem-cell-derived alpha , beta , and enterochromaffin cells, respectively. D. Heatmap showing the expression of the top ten marker genes of each time point. lncRNA genes are indicated by arrows. E. Enriched GO terms of marker genes of each time point. F. UMAP visualization of the expression of genes related to endoderm formation. G. Violin plots showing the expression of lncRNA genes that listed in the top 10 marker genes of each time point Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36907881), licensed under a CC-BY license. Not internally tested by R&D Systems.

FOXA2 drives the p3-specific chromatin accessibility program(A) Footprinting score for the FOX motif is highest in p3 samples.(B) Foxa2 expression in p3 NPs suggests it is the most likely candidate to drive the footprinting signal.(C) Average ATAC-seq accessibility at FOXA2 ChIP-seq peaks13 in the indicated samples shows these regions are highly accessible in p3 NPs.(D) Normalized FOXA2 ChIP-seq coverage showing accessibility in p3-specific elements from the groups of ATAC-seq elements in the indicated clusters from Figure 3B.(E) Genetic lineage tracing indicates that cells that expressed Foxa2 at E8.5 (tamoxifen administration) have contributed to the p3 progenitor and V3 neuronal cell types by E11.5 (red arrows).(F) Quantifications of p3 and V3 cells expressing tdTomato in embryos collected after tamoxifen administration at the indicated times. Biological replicates: n = 2 for E7.5, n = 4 for E8.5, n = 3 for E9.5.(G) Foxa2−/− ES cells fail to generate p3 NPs when exposed to 500 nM SAG.(H) Representative flow cytometry plots of the quantifications in (G) showing a marked reduction in p3 NPs from Foxa2−/− ES cells compared with wild type. Cells are gated for SOX2+ live neural progenitors.(I) Representative immunohistochemistry staining for SOX2, OLIG2 and NKX2,2 showing reduced number of cells expressing NKX2.2 in Foxa2−/− mutant cells at day 6 of differentiation treated with 500 nM SAG.Scale bars, 50 μm.See also Figure S4. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36516856), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

HNF-3 beta/FoxA2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HNF‑3 beta /FoxA2
  Met242-Ser457
  Accession # Q9Y261
• Specificity: Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 10-50 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 beta/FoxA2 Antibody [Unconjugated]

• forkhead box A2
• Forkhead box protein A2
• FoxA2
• hepatic nuclear factor-3-beta
• hepatocyte nuclear factor 3, beta
• hepatocyte nuclear factor 3-beta
• HNF3 beta
• HNF-3 beta
• HNF-3B
• HNF-3-beta
• HNF3BTCF-3B
• MGC19807
• TCF3B
• Transcription factor 3B

Background

HNF-3 beta , also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HNF-3 beta/FoxA2 Antibody (AF2400)(106)

Publications using AF2400

• Star?evi?, P;Buono, MF;Pol, F;Bartelds, B;Schipper, HS;Sluijter, JPG;Doevendans, P;Stillitano, F; Generation and characterization of induced pluripotent stem cell (iPSC) lines from two symptomatic carriers of pathogenic TNNI3 variants and two non-carrier relatives Stem cell research 2026-03-04 [PMID: 41814115] (Flow Cytometry, Immunocytochemistry, Human)
• Yang, F;Pavon, N;Matsubara, T;Sebastian, R;Martinez-Martin, B;Pak, C;Sun, Y;Kim, DH; Distinct spatial patterning and transcriptomic landscapes of human neural organoids by localized delivery of morphogens Cell stem cell 2026-03-05 [PMID: 41734763] (Immunohistochemistry, Human)
• Sawamoto, N;Doi, D;Nakanishi, E;Sawamura, M;Kikuchi, T;Yamakado, H;Taruno, Y;Shima, A;Fushimi, Y;Okada, T;Kikuchi, T;Morizane, A;Hiramatsu, S;Anazawa, T;Shindo, T;Ueno, K;Morita, S;Arakawa, Y;Nakamoto, Y;Miyamoto, S;Takahashi, R;Takahashi, J; Phase I/II trial of iPS-cell-derived dopaminergic cells for Parkinson's disease Nature 2025-04-16 [PMID: 40240591] (Immunohistochemistry, Rat)
• Kim, TW;Piao, J;Bocchi, VD;Koo, SY;Choi, SJ;Chaudhry, F;Yang, D;Cho, HS;Hergenreder, E;Perera, LR;Joshi, S;Mrad, ZA;Claros, N;Donohue, SA;Frank, AK;Walsh, R;Mosharov, EV;Betel, D;Tabar, V;Studer, L; Enhanced yield and subtype identity of hPSC-derived midbrain dopamine neuron by modulation of WNT and FGF18 signaling bioRxiv : the preprint server for biology 2025-01-06 [PMID: 39829874] (Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Human, Xenograft)
• Atash, A;Cramer, MJ;Mees, B;Doevendans, PA;Sluijter, JPG;Stillitano, F; Generation and characterization of novel induced pluripotent stem cell (iPSC) lines derived from three symptomatic carriers of a pathogenic MYH11 variant and two non-carrier relatives Stem cell research 2024-12-12 [PMID: 39705883] (Immunocytochemistry, Human)
• Selvitella, S;Franco, MP;Aran, B;Veiga, A;Kuebler, B; Generation of the CTRL EiPS J9 mR6F-8 iPSC line derived from healthy human outgrowth blood endothelial cells (BOECs) using mRNA reprogramming methodology Stem cell research 2024-11-09 [PMID: 39541769] (Flow Cytometry, Immunocytochemistry, Human)
• Nouruzi, S;Namekawa, T;Tabrizian, N;Kobelev, M;Sivak, O;Scurll, JM;Cui, CJ;Ganguli, D;Zoubeidi, A; ASCL1 regulates and cooperates with FOXA2 to drive terminal neuroendocrine phenotype in prostate cancer JCI insight 2024-12-06 [PMID: 39470735] (Chromatin Immunoprecipitation Sequencing, Human)
• Dattani, A;Corujo-Simon, E;Radley, A;Heydari, T;Taheriabkenar, Y;Carlisle, F;Lin, S;Liddle, C;Mill, J;Zandstra, PW;Nichols, J;Guo, G; Naive pluripotent stem cell-based models capture FGF-dependent human hypoblast lineage specification Cell stem cell 2024-05-24 [PMID: 38823388] (Immunohistochemistry, Human)
• Daya, NM;Döring, K;Zhuge, H;Volke, L;Stab, V;Dietz, J;Athamneh, M;Roos, A;Zaehres, H;Güttsches, AK;Mavrommatis, L;Vorgerd, M; Generation of two hiPSCs lines of two patients carrying truncating mutations in the dimerization domain of filamin C Stem cell research 2024-01-27 [PMID: 38309149] (Flow Cytometry, Immunocytochemistry, Human)
• Kojima, R;Paslawski, W;Lyu, G;Arenas, E;Zhang, X;Svenningsson, P; Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson's Disease Patients with GBA1 Mutations International journal of molecular sciences 2024-01-04 [PMID: 38203854] (Immunocytochemistry, Human)
• Maimaitili, M;Chen, M;Febbraro, F;Ucuncu, E;Kelly, R;Niclis, JC;Christiansen, JR;Mermet-Joret, N;Niculescu, D;Lauritsen, J;Iannielli, A;Klæstrup, IH;Jensen, UB;Qvist, P;Nabavi, S;Broccoli, V;Nykjær, A;Romero-Ramos, M;Denham, M; Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells Nature communications 2023-12-05 [PMID: 38052784] (Immunohistochemistry, Immunocytochemistry, Human, Xenograft)
• Pavlinov, I;Tambe, M;Abbott, J;Nguyen, HN;Xu, M;Pradhan, M;Farkhondeh, A;Zheng, W; In depth characterization of midbrain organoids derived from wild type iPSC lines PloS one 2023-10-20 [PMID: 37862312] (Human)
• Boeing, A;Mavrommatis, L;Daya, NM;Zhuge, H;Volke, L;Kocabas, A;Kneifel, M;Athamneh, M;Krause, K;Südkamp, N;Döring, K;Theiss, C;Roos, A;Zaehres, H;Güttsches, AK;Vorgerd, M; Generation of two human iPSC lines (HIMRi002-A and HIMRi003-A) derived from Caveolinopathy patients with rippling muscle disease Stem cell research 2023-10-04 [PMID: 37839261] (ICC, Human)
• Balducci, V;Scardigli, F;Harakalova, M;Peter van Tintelen, J;Doevendans, PA;Costa, KD;Turnbull, IC;P G Sluijter, J;Stillitano, F; Generation and characterization of novel human induced pluripotent stem cell (iPSC) lines originating from five asymptomatic individuals carrying the PLN-R14del pathogenic variant and a non-carrier relative Stem cell research 2023-09-21 [PMID: 37748331] (ICC, Human)
• Souralova, T;Hulinova, D;Jeseta, M;Ventruba, P;Hampl, A;Koutna, I; Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells Scientific reports 2023-09-12 [PMID: 37700192] (Immunocytochemistry, Human)
• Montero-Calle, P;Flandes-Iparraguirre, M;Kuebler, B;Arán, B;Larequi, E;Anaut, I;Coppiello, G;Aranguren, XL;Veiga, A;Elorz, MTB;de Yébenes, MG;Gavira, JJ;Prósper, F;Iglesias-García, O;Vega, MMM; Generation of an induced pluripotent stem cell line (ESi107-A) from a transthyretin amyloid cardiomyopathy (ATTR-CM) patient carrying a p.Ser43Asn mutation in the TTR gene Stem cell research 2023-09-01 [PMID: 37660554] (ICC, Human)
• Lin Feng, Da Li, Yao Tian, Chengshun Zhao, Yun Sun, Xiaolong Kou et al. One-step cell biomanufacturing platform: porous gelatin microcarrier beads promote human embryonic stem cell-derived midbrain dopaminergic progenitor cell differentiation in vitro and survival after transplantation in vivo Neural Regeneration Research 2024-02-01 [PMID: 37488911]
• Muwan Chen, Jonathan Christos Niclis, Mark Denham Protocol for generating reproducible miniaturized controlled midbrain organoids STAR Protocols 2023-09-01 [PMID: 37481727]
• Takeshi Kato, Kaneyasu Nishimura, Masahiro Hirao, Shun Shimohama, Kazuyuki Takata Expression and role of nicotinic acetylcholine receptors during midbrain dopaminergic neuron differentiation from human induced pluripotent stem cells Neuropsychopharmacology Reports 2023-06-27 [PMID: 37366076]
• Kaneyasu Nishimura, Emilía Sif Ásgrímsdóttir, Shanzheng Yang, Ernest Arenas A protocol for the differentiation of human embryonic stem cells into midbrain dopaminergic neurons STAR Protocols 2023-09-01 [PMID: 37310863]
• H Bogetofte, BJ Ryan, P Jensen, SI Schmidt, DLE Vergoossen, MB Barnkob, LN Kiani, U Chughtai, R Heon-Rober, MC Caiazza, W McGuinness, R Márquez-Gó, J Vowles, FS Bunn, J Brandes, P Kilfeather, JP Connor, HJR Fernandes, TM Caffrey, M Meyer, SA Cowley, MR Larsen, R Wade-Marti Post-translational proteomics platform identifies neurite outgrowth impairments in Parkinson's disease GBA-N370S dopamine neurons Cell Reports, 2023-03-03;42(3):112180. 2023-03-03 [PMID: 36870058] (ICC, Human)
• EY Akda?, S Turan, D Guhathakur, A Ekici, S Salar, DC Lie, B Winner, A Fejtova CRISPR/Cas9-mediated generation of hESC lines with homozygote and heterozygote p.R331W mutation in CTBP1 to model HADDTS syndrome Stem Cell Research, 2022-12-30;67(0):103012. 2022-12-30 [PMID: 36610307] (Flow Cytometry, ICC, Human)
• Zachary T. Olmsted, Maria Belen Paredes-Espinosa, Janet L. Paluh Generation of human elongating multi-lineage organized cardiac gastruloids STAR Protocols 2022-12-16 [PMID: 36595961]
• L Yang, S Lu, Z Yang, J Yao, P Zhou, M Zhao Generation of an induced pluripotent stem cell line (IPCASi001-A) from an autism spectrum disorder individual without intellectual disability Stem Cell Research, 2022-12-05;66(0):102994. 2022-12-05 [PMID: 36502620] (ICC, Human)
• CB Pantazis, A Yang, E Lara, JA McDonough, C Blauwendra, L Peng, H Oguro, J Kanaujiya, J Zou, D Sebesta, G Pratt, E Cross, J Blockwick, P Buxton, L Kinner-Bib, C Medura, C Tompkins, S Hughes, M Santiana, F Faghri, MA Nalls, D Vitale, S Ballard, YA Qi, DM Ramos, KM Anderson, J Stadler, P Narayan, J Papademetr, L Reilly, MP Nelson, S Aggarwal, LU Rosen, P Kirwan, V Pisupati, SL Coon, SW Scholz, T Priebe, M Öttl, J Dong, M Meijer, LJM Janssen, VS Lourenco, R van der Ka, D Crusius, D Paquet, AC Raulin, G Bu, A Held, BJ Wainger, RMC Gabriele, JM Casey, S Wray, D Abu-Bonsra, CL Parish, MS Beccari, DW Cleveland, E Li, IVL Rose, M Kampmann, C Calatayud, P Verstreken, L Heinrich, MY Chen, B Schüle, D Dou, ELF Holzbaur, MC Zanellati, R Basundra, M Deshmukh, S Cohen, R Khanna, M Raman, ZS Nevin, M Matia, J Van Lent, V Timmerman, BR Conklin, K Johnson Ch, K Zhang, S Funes, DA Bosco, L Erlebach, M Welzer, D Kronenberg, G Lyu, E Arenas, E Coccia, L Sarrafha, T Ahfeldt, JC Marioni, WC Skarnes, MR Cookson, ME Ward, FT Merkle A reference human induced pluripotent stem cell line for large-scale collaborative studies Cell Stem Cell, 2022-12-01;29(12):1685-1702.e22. 2022-12-01 [PMID: 36459969] (ICC, Human)
• K Ruangrung, W Chakritbud, S Thongon, S Rungarunle, M Wattanapan, C Boonarkart, O Suptawiwat, N Sirinontha, DR Smith, P Auewarakul Analysis of Influenza A virus infection in human induced pluripotent stem cells (hiPSCs) and their derivatives Virus research, 2022-11-19;323(0):199009. 2022-11-19 [PMID: 36414188] (IHC, Human)
• C Cattelani, I Battistell, F Di Leva, G Fioravanti, F Benedicent, F Stanzial, C Schwienbac, F Fanelli, PP Pramstalle, AA Hicks, L Conti, C Corti Induced Pluripotent Stem Cell (iPSC) Lines from a Family with Resistant Epileptic Encephalopathy Caused by Compound Heterozygous Mutations in SZT2 Gene International Journal of Molecular Sciences, 2022-10-28;23(21):. 2022-10-28 [PMID: 36361881] (ICC, Human)
• Tereza Souralova, Daniela Rehakova, Michal Jeseta, Lenka Tesarova, Jindrich Beranek, Pavel Ventruba et al. The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy International Journal of Molecular Sciences 2022-10-18 [PMID: 36293356]
• Yoshiki Nakashima, Chika Miyagi-Shiohira, Issei Saitoh, Masami Watanabe, Masayuki Matsushita, Masayoshi Tsukahara et al. Induced hepatic stem cells are suitable for human hepatocyte production iScience 10/21/2022 [PMID: 36147945]
• Hunter J. Johnson, Saheli Chakraborty, Riya J. Muckom, Nitash P. Balsara, David V. Schaffer A scalable and tunable thermoreversible polymer for 3D human pluripotent stem cell biomanufacturing iScience 2022-10-21 [PMID: 36147944]
• Trinh LT, Osipovich AB, Sampson L et al. Differential regulation of alternate promoter regions in Sox17 during endodermal and vascular endothelial development iScience 9/16/2022 [PMID: 36046192]
  
  Details:
  20 ng/ml used, mESCs were differentiated to vascular endothelial cells
• Olmsted ZT, Paluh JL. A combined human gastruloid model of cardiogenesis and neurogenesis iScience 2022-06-17 [PMID: 35721464]
• T Tada, H Ohnishi, N Yamamoto, F Kuwata, Y Hayashi, H Okuyama, T Morino, Y Kasai, H Kojima, K Omori Transplantation of a human induced pluripotent stem cell-derived airway epithelial cell sheet into the middle ear of rats Regenerative Therapy, 2022-01-14;19(0):77-87. 2022-01-14 [PMID: 35097166] (ICC, Human)
• P Aldrin-Kir, M Åkerblom, T Cardoso, S Nolbrant, AF Adler, X Liu, A Heuer, M Davidsson, M Parmar, T Björklund A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants Stem Cell Reports, 2021-12-30;17(1):159-172. 2021-12-30 [PMID: 34971563] (ICC, IHC, Human, Rat, Transgenic Rat)
• Angelique N. Masibag, Christopher J. Bergin, Joshua R. Haebe, Aïcha Zouggar, Muhammad S. Shah, Tamara Sandouka et al. Pharmacological targeting of Sam68 functions in colorectal cancer stem cells iScience 2021-12-17 [PMID: 34877499]
• RJ Geusz, A Wang, DK Lam, NK Vinckier, KD Alysandrat, DA Roberts, J Wang, S Kefalopoul, A Ramirez, Y Qiu, J Chiou, KJ Gaulton, B Ren, DN Kotton, M Sander Sequence logic at enhancers governs a dual mechanism of endodermal organ fate induction by FOXA pioneer factors Nature Communications, 2021-11-17;12(1):6636. 2021-11-17 [PMID: 34789735] (ChIP, IHC, Human)
• Miranda Robbins, Venkat Pisupati, Roberta Azzarelli, Samer I. Nehme, Roger A. Barker, Ljiljana Fruk et al. Biofunctionalised bacterial cellulose scaffold supports the patterning and expansion of human embryonic stem cell-derived dopaminergic progenitor cells Stem Cell Research & Therapy 2021-12-01 [PMID: 34774094] (Immunocytochemistry, Human)
• T Bluhmki, S Traub, AK Müller, S Bitzer, E Schruf, MT Bammert, M Leist, F Gantner, JP Garnett, R Heilker Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96?Transwell air-liquid interface model Scientific Reports, 2021-08-23;11(1):17028. 2021-08-23 [PMID: 34426605] (IHC, Human)
• A Fütterer, A Talavera-G, T Pons, J de Celis, J Gutiérrez, V Domínguez, C Martínez-A Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels Cell Death & Disease, 2021-06-21;12(7):637. 2021-06-21 [PMID: 34155199] (IHC, Human)
• S Chen, Y Han, L Yang, T Kim, M Nair, O Harschnitz, P Wang, J Zhu, SY Koo, X Tang, L Lacko, V Chandar, Y Bram, T Zhang, W Zhang, F He, J Caicedo, Y Huang, T Evans, P van der Va, MJ Titulaer, JKH Spoor, RL Furler, P Canoll, J Goldman, S Przedborsk, R Schwartz, D Ho, L Studer SARS-CoV-2 Infection Causes Dopaminergic Neuron Senescence Research square, 2021-05-21;0(0):. 2021-05-21 [PMID: 34031650] (IHC, Human)
• H Mou, Y Yang, MA Riehs, J Barrios, M Shivaraju, AL Haber, DT Montoro, K Gilmore, EA Haas, B Paunovic, J Rajagopal, SO Vargas, RL Haynes, A Fine, WV Cardoso, X Ai Airway basal stem cells generate distinct subpopulations of PNECs Cell Reports, 2021-04-20;35(3):109011. 2021-04-20 [PMID: 33882306] (ICC, IHC, Human)
• G Guo, GG Stirparo, SE Strawbridg, D Spindlow, J Yang, J Clarke, A Dattani, A Yanagida, MA Li, S Myers, BN Özel, J Nichols, A Smith Human naive epiblast cells possess unrestricted lineage potential Cell Stem Cell, 2021-04-07;0(0):. 2021-04-07 [PMID: 33831366] (ICC, Human)
• Y Tao, SC Vermilyea, M Zammit, J Lu, M Olsen, JM Metzger, L Yao, Y Chen, S Phillips, JE Holden, V Bondarenko, WF Block, TE Barnhart, N Schultz-Da, K Brunner, H Simmons, BT Christian, ME Emborg, SC Zhang Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys Nature Medicine, 2021-03-01;0(0):. 2021-03-01 [PMID: 33649496] (ICC, IHC, Primate - Rhesus macaque)
• TW Kim, J Piao, SY Koo, S Kriks, SY Chung, D Betel, ND Socci, SJ Choi, S Zabierowsk, BN Dubose, EJ Hill, EV Mosharov, S Irion, MJ Tomishima, V Tabar, L Studer Biphasic Activation of WNT Signaling Facilitates the Derivation of Midbrain Dopamine Neurons from hESCs for Translational Use Cell Stem Cell, 2021-02-04;28(2):343-355.e5. 2021-02-04 [PMID: 33545081] (ICC, Human)
• J Piao, S Zabierowsk, BN Dubose, EJ Hill, M Navare, N Claros, S Rosen, K Ramnarine, C Horn, C Fredrickso, K Wong, B Safford, S Kriks, A El Maarouf, U Rutishause, C Henchcliff, Y Wang, I Riviere, S Mann, V Bermudez, S Irion, L Studer, M Tomishima, V Tabar Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01 Cell Stem Cell, 2021-02-04;28(2):217-229.e7. 2021-02-04 [PMID: 33545080] (Flow Cytometry, ICC, IHC, Human, Mouse)
• B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, R Corominas, I Cuscó Generation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering Williams-Beuren syndrome (7q11.23 deletion) Stem Cell Research, 2020-11-16;49(0):102087. 2020-11-16 [PMID: 33370870] (Flow Cytometry, Human)
• B Kuebler, B Aran, R Flores, LA Pérez-Jura, A Veiga, I Cuscó, R Corominas Derivation of induced pluripotent stem cells (iPSCs) by retroviral transduction of skin fibroblasts from four patients suffering 7q11.23 microduplication syndrome Stem Cell Res, 2020-11-19;49(0):102092. 2020-11-19 [PMID: 33254092] (ICC, Human)
• M Inglés-Fer, M Martin-Ina, L Herrera, M Villaverde, S Santos, MA Vesga, E Garreta, I Martín-Rui, AM Aransay, J Anguita, B Barreña, LM Allende, LI Gonzalez-G, C Eguizabal Generation, establishment and characterization of a pluripotent stem cell line (CVTTHi001-A) from primary fibroblasts isolated from a patient with activated PI3 kinase delta syndrome (APDS2) Stem Cell Res, 2020-11-07;49(0):102082. 2020-11-07 [PMID: 33221676] (ICC, Human)
• Patrick Walsh, Vincent Truong, Sushmita Nayak, Marietta Saldías Montivero, Walter C. Low, Ann M. Parr et al. Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population Stem Cells 2020-11-01 [PMID: 32745311]
• E Naumovska, G Aalderink, C Wong Valen, K Kosim, A Nicolas, S Brown, P Vulto, KS Erdmann, D Kurek Direct On-Chip Differentiation of Intestinal Tubules from Induced Pluripotent Stem Cells Int J Mol Sci, 2020-07-14;21(14):. 2020-07-14 [PMID: 32674311] (ICC, Human)
• Z Yang, X Jiang, Z Zhang, Z Zhao, W Xing, Y Liu, X Jiang, H Zhao HDAC3-dependent transcriptional repression of FOXA2 regulates FTO/m6A/MYC signaling to contribute to the development of gastric cancer Cancer Gene Ther., 2020-07-13;0(0):. 2020-07-13 [PMID: 32655129] (Immunoprecipitation, Mouse)
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020-06-19;27(1):125-136.e7. 2020-06-19 [PMID: 32579880] (ICC, IHC, Human)
• L Yang, Y Han, BE Nilsson-Pa, V Gupta, P Wang, X Duan, X Tang, J Zhu, Z Zhao, F Jaffré, T Zhang, TW Kim, O Harschnitz, D Redmond, S Houghton, C Liu, A Naji, G Ciceri, S Guttikonda, Y Bram, DT Nguyen, M Cioffi, V Chandar, DA Hoagland, Y Huang, J Xiang, H Wang, D Lyden, A Borczuk, HJ Chen, L Studer, FC Pan, DD Ho, BR tenOever, T Evans, RE Schwartz, S Chen A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids Cell Stem Cell, 2020;27(1):125-136.e7. 2020 [PMID: 32579880] (IHC, Human)
• S Yoshida, H Miwa, T Kawachi, S Kume, K Takahashi Generation of intestinal organoids derived from human pluripotent stem cells for drug testing Sci Rep, 2020-04-06;10(1):5989. 2020-04-06 [PMID: 32249832] (ICC, Human)
• NV Fuchs, M Schieck, M Neuenkirch, C Tondera, H Schmitz, L Wendeburg, D Steinemann, C Elpers, F Rutsch, R König Generation of three induced pluripotent cell lines (iPSCs) from an Aicardi-Gouti�res syndrome (AGS) patient harboring a deletion in the genomic locus of the sterile alpha motif and HD domain containing protein 1 (SAMHD1) Stem Cell Res, 2020-01-09;43(0):101697. 2020-01-09 [PMID: 32062129] (ICC, Human)
• YY Kim, H Kim, CS Suh, HC Liu, Z Rosenwaks, SY Ku Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model Int J Mol Sci, 2020-01-24;21(3):. 2020-01-24 [PMID: 31991577] (ICC, Human)
• Da Li, T Cao, X Sun, S Jin, Di Xie, X Huang, X Yang, GG Carmichael, HS Taylor, S Diano, Y Huang Hepatic TET3 contributes to type-2 diabetes by inducing the HNF4alpha fetal isoform Nat Commun, 2020;11(1):342. 2020 [PMID: 31953394] (ChIP, Mouse)
• Da Li, T Cao, X Sun, S Jin, Di Xie, X Huang, X Yang, GG Carmichael, HS Taylor, S Diano, Y Huang Hepatic TET3 contributes to type-2 diabetes by inducing the HNF4alpha fetal isoform Nat Commun, 2020-01-17;11(1):342. 2020-01-17 [PMID: 31953394] (ChIP, Mouse)
• Y Hu, CM Domínguez, J Bauer, S Weigel, A Schipperge, C Oelschlaeg, N Willenbach, S Keppler, M Bastmeyer, S Hei beta ler, C Wöll, T Scharnwebe, KS Rabe, CM Niemeyer Carbon-nanotube reinforcement of DNA-silica nanocomposites yields programmable and cell-instructive biocoatings Nat Commun, 2019-12-04;10(1):5522. 12/4/2019 [PMID: 31797918]
• N Bredenkamp, J Yang, J Clarke, GG Stirparo, F von Meyenn, S Dietmann, D Baker, R Drummond, Y Ren, D Li, C Wu, M Rostovskay, S Eminli-Mei, A Smith, G Guo Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency Stem Cell Reports, 2019-11-07;13(6):1083-1098. 2019-11-07 [PMID: 31708477] (ICC, Human)
• M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons Cell Stem Cell, 2019-09-19;25(4):514-530.e8. 2019-09-19 [PMID: 31543366] (ICC, Human)
• Hannah T. Stuart, Giuliano G. Stirparo, Tim Lohoff, Lawrence E. Bates, Masaki Kinoshita, Chee Y. Lim et al. Distinct Molecular Trajectories Converge to Induce Naive Pluripotency Cell Stem Cell 2019-09-05 [PMID: 31422912]
• K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019-07-09;28(2):382-393.e7. 2019-07-09 [PMID: 31291575] (Immunoprecipitation, Western Blot, Human)
• R Yokoi, M Okabe, N Matsuda, A Odawara, A Karashima, I Suzuki Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons Front Neurosci, 2019-05-29;13(0):554. 2019-05-29 [PMID: 31191238] (ICC, Human)
• C Calatayud, G Carola, I Fernández-, M Valtorta, S Jiménez-De, M Díaz, J Soriano-Fr, G Cappellett, J García-San, Á Raya, A Consiglio CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons Sci Rep, 2019-05-02;9(1):6811. 2019-05-02 [PMID: 31048719] (ICC, Human)
• Natalie Connor-Robson, Heather Booth, Jeffrey G. Martin, Benbo Gao, Kejie Li, Natalie Doig et al. An integrated transcriptomics and proteomics analysis reveals functional endocytic dysregulation caused by mutations in LRRK2 Neurobiology of Disease 2019-07-01 [PMID: 30954703]
• T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival Nat Commun, 2018-11-16;9(1):4815. 2018-11-16 [PMID: 30446643] (ICC, Human)
• H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors Stem Cell Res Ther, 2018-10-25;9(1):277. 2018-10-25 [PMID: 30359326] (ICC, Human)
• E Senís, L Mosteiro, S Wilkening, E Wiedtke, A Nowrouzi, S Afzal, R Fronza, H Landerer, M Abad, D Niopek, M Schmidt, M Serrano, D Grimm AAVvector-mediated in vivo reprogramming into pluripotency Nat Commun, 2018-07-09;9(1):2651. 2018-07-09 [PMID: 29985406] (ICC, Mouse)
• Meredith M. Course, Chung-Han Hsieh, Pei-I Tsai, Jennifer A. Codding-Bui, Atossa Shaltouki, Xinnan Wang Live Imaging Mitochondrial Transport in Neurons Neuromethods 2017-03-18 [PMID: 29977105]
• B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS) Stem Cell Res, 2017-09-09;0(0):. 2017-09-09 [PMID: 29150092] (ICC, Human)
• L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines Stem Cell Res, 2017-06-07;24(0):155-159. 2017-06-07 [PMID: 29034884] (ICC, Human)
• TS Cliff, T Wu, BR Boward, A Yin, H Yin, JN Glushka, JH Prestegaar, S Dalton MYC Controls Human Pluripotent Stem Cell Fate Decisions through Regulation of Metabolic Flux Cell Stem Cell, 2017-09-28;21(4):502-516.e9. 2017-09-28 [PMID: 28965765] (Immunocytochemistry, Human)
• KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells Stem Cell Reports, 2017-06-08;0(0):. 2017-06-08 [PMID: 28602613] (ICC, Human)
• Y Kondo, T Toyoda, R Ito, M Funato, Y Hosokawa, S Matsui, T Sudo, M Nakamura, C Okada, X Zhuang, A Watanabe, A Ohta, N Inagaki, K Osafune Identification of a small molecule that facilitates the differentiation of human iPSCs/ESCs and mouse embryonic pancreatic explants into pancreatic endocrine cells Diabetologia, 2017-05-22;0(0):. 2017-05-22 [PMID: 28534195] (ICC, Human, Mouse)
• JH Lee, S Laronde, TJ Collins, Z Shapovalov, B Tanasijevi, JD McNicol, A Fiebig-Com, YD Benoit, JB Lee, RR Mitchell, M Bhatia Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency Cell Rep, 2017-04-04;19(1):20-35. 2017-04-04 [PMID: 28380358] (Flow Cytometry, ICC, Human)
• F Sampazioti, MC de Brito, I Geti, A Bertero, NR Hannan, L Vallier Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells Nat Protoc, 2017-03-23;12(4):814-827. 2017-03-23 [PMID: 28333915] (Flow Cytometry, Human)
• Tetsuhiro Kikuchi, Asuka Morizane, Daisuke Doi, Keisuke Okita, Masato Nakagawa, Hodaka Yamakado et al. Idiopathic Parkinson's disease patient‐derived induced pluripotent stem cells function as midbrain dopaminergic neurons in rodent brains Journal of Neuroscience Research 2017-09-01 [PMID: 28233934] (Immunohistochemistry, Human)
• A Shiraishi, K Muguruma, Y Sasai Generation of thalamic neurons from mouse embryonic stem cells Development, 2017-02-20;0(0):. 2017-02-20 [PMID: 28219951] (ICC, Mouse)
• Leslie E Rogler Triple Staining Including FOXA2 Identifies Stem Cell Lineages Undergoing Hepatic and Biliary Differentiation in Cirrhotic Human Liver J. Histochem. Cytochem., 2016-11-24;0(0):. 2016-11-24 [PMID: 27879410] (ICC, Human)
• Cell Expansion During Directed Differentiation Of Stem Cells Toward The Hepatic Lineage Stem Cells Dev., 2016-11-01;0(0):. 2016-11-01 [PMID: 27806669] (CyTof, Human)
• Microfabric Vessels for Embryoid Body Formation and Rapid Differentiation of Pluripotent Stem Cells Sci Rep, 2016-08-10;6(0):31063. 2016-08-10 [PMID: 27507707] (IHC, Mouse)
• Rubén Fernández‐Santiago, Iria Carballo‐Carbajal, Giancarlo Castellano, Roger Torrent, Yvonne Richaud, Adriana Sánchez‐Danés et al. Aberrant epigenome in iPSC ‐derived dopaminergic neurons from Parkinson's disease patients EMBO Molecular Medicine 2015-12-01 [PMID: 26516212]
• Tsankov A, Akopian V, Pop R, Chetty S, Gifford C, Daheron L, Tsankova N, Meissner A A qPCR ScoreCard quantifies the differentiation potential of human pluripotent stem cells. Nat Biotechnol, 2015-10-26;33(11):1182-92. 2015-10-26 [PMID: 26501952] (IHC-P, Human)
• Filipe V. Jacinto, Chris Benner, Martin W. Hetzer The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing Genes & Development 2015-06-15 [PMID: 26080816]
• Villa-Diaz L, Kim J, Lahann J, Krebsbach P Derivation and long-term culture of transgene-free human induced pluripotent stem cells on synthetic substrates. Stem Cells Transl Med, 2014-10-13;3(12):1410-7. 2014-10-13 [PMID: 25313201] (ICC, Human)
• Hartfield , Elizabet, Yamasaki-Mann , Michiko, Ribeiro Fernandes , Hugo J, Vowles , Jane, James , William, Cowley , Sally A, Wade-Martins , Richard Physiological characterisation of human iPS-derived dopaminergic neurons. PLoS ONE, 2014-02-21;9(2):e87388. 2014-02-21 [PMID: 24586273] (IHC-Fr, Human)
• Gasimli L, Hickey AM, Yang B et al. Changes in glycosaminoglycan structure on differentiation of human embryonic stem cells towards mesoderm and endoderm lineages. BBA General Subjects. 1/1/2014 [PMID: 24412195]
• Lynsey Howard, Ruth M Mackenzie, Nikolay A Pchelintsev, Tony McBryan, John D McClure, Martin W McBride et al. Profiling of transcriptional and epigenetic changes during directed endothelial differentiation of human embryonic stem cells identifies FOXA2 as a marker of early mesoderm commitment Stem Cell Research & Therapy 2013-06-01 [PMID: 23618383]
• Szablowska-Gadomska , Ilona, Sypecka , Joanna, Zayat , Valery, Podobinska , Martyna, Pastwinska , Anna, Pienkowska-Grela , Barbara, Buzanska , Leonora Treatment with small molecules is an important milestone towards the induction of pluripotency in neural stem cells derived from human cord blood. Acta Neurobiol Exp (Wars), 2012-01-01;72(4):337-50. 2012-01-01 [PMID: 23377265] (ICC, Human)
• Montserrat N, Ramirez-Bajo MJ, Xia Y, Sancho-Martinez I, Moya-Rull D, Miquel-Serra L, Yang S, Nivet E, Cortina C, Gonzalez F, Izpisua Belmonte JC, Campistol JM Generation of induced pluripotent stem cells from human renal proximal tubular cells with only two transcription factors, oct4 and sox2. J. Biol. Chem., 2012-05-21;287(29):24131-8. 2012-05-21 [PMID: 22613719] (ICC, Human)
• Prabakar K, Dominguez-Bendala J, Molano R, Pileggi A, Villate S, Ricordi C, Inverardi L Generation of glucose-responsive, insulin-producing cells from human umbilical cord blood-derived mesenchymal stem cells. Cell Transplant, 2011-12-21;21(6):1321-39. 2011-12-21 [PMID: 22195604] (ICC, Human)
• Eguizabal C, Montserrat N, Vassena R, Barragan M, Garreta E, Garcia-Quevedo L, Vidal F, Giorgetti A, Veiga A, Belmonte JC Complete meiosis from human induced pluripotent stem cells. Stem Cells, 2011-08-01;29(8):1186-95. 2011-08-01 [PMID: 21681858] (ICC, Human)
• Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. J. Cell. Sci., 2011-05-24;124(0):1992-2000. 2011-05-24 [PMID: 21610099] (ICC, Human)
• Guang-Hui Liu, Basam Z. Barkho, Sergio Ruiz, Dinh Diep, Jing Qu, Sheng-Lian Yang et al. Recapitulation of premature ageing with iPSCs from Hutchinson–Gilford progeria syndrome Nature 2011-04-14 [PMID: 21346760]
• Jiang W, Sui X, Zhang D, Liu M, Ding M, Shi Y, Deng H CD24: a novel surface marker for PDX1-positive pancreatic progenitors derived from human embryonic stem cells. Stem Cells, 2011-04-01;29(4):609-17. 2011-04-01 [PMID: 21308865] (ICC, Human)
• Klim JR, Li L, Wrighton PJ A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat. Methods, 2010-11-14;7(12):989-94. 2010-11-14 [PMID: 21076418] (ICC, Human)
• Chayosumrit M, Tuch B, Sidhu K Alginate microcapsule for propagation and directed differentiation of hESCs to definitive endoderm. Biomaterials, 2009-10-14;31(3):505-14. 2009-10-14 [PMID: 19833385] (ICC, Western Blot, Human)
• Kawamura T, Suzuki J, Wang YV, Menendez S, Morera LB, Raya A, Wahl GM, Belmonte JC Linking the p53 tumour suppressor pathway to somatic cell reprogramming. Nature, 2009-08-09;460(7259):1140-4. 2009-08-09 [PMID: 19668186] (ICC, Mouse)
• Raya A, Rodriguez-Piza I, Guenechea G, Vassena R, Navarro S, Barrero MJ, Consiglio A, Castella M, Río P, Sleep E, Gonzalez F, Tiscornia G, Garreta E, Aasen T, Veiga A, Verma IM, Surralles J, Bueren J, Belmonte JC Disease-corrected haematopoietic progenitors from Fanconi anaemia induced pluripotent stem cells. Nature, 2009-05-31;460(7251):53-9. 2009-05-31 [PMID: 19483674] (ICC, Human)
• Zhang D, Jiang W, Liu M, Sui X, Yin X, Chen S, Shi Y, Deng H Highly efficient differentiation of human ES cells and iPS cells into mature pancreatic insulin-producing cells. Cell Res., 2009-04-01;19(4):429-38. 2009-04-01 [PMID: 19255591] (Flow Cytometry, ICC, Human)
• Conrad S, Renninger M, Hennenlotter J, Wiesner T, Just L, Bonin M, Aicher W, Buhring HJ, Mattheus U, Mack A, Wagner HJ, Minger S, Matzkies M, Reppel M, Hescheler J, Sievert KD, Stenzl A, Skutella T Generation of pluripotent stem cells from adult human testis. Nature, 2008-10-08;456(7220):344-9. 2008-10-08 [PMID: 18849962] (ICC, Human)
• Nakanishi M, Kurisaki A, Hayashi Y, Warashina M, Ishiura S, Kusuda-Furue M, Asashima M Directed induction of anterior and posterior primitive streak by Wnt from embryonic stem cells cultured in a chemically defined serum-free medium. FASEB J., 2008-09-22;23(1):114-22. 2008-09-22 [PMID: 18809738] (ICC, Human)
• Zhang P, Li J, Tan Z, Wang C, Liu T, Chen L, Yong J, Jiang W, Sun X, DU L, Ding M, Deng H Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood, 2007-11-27;111(4):1933-41. 2007-11-27 [PMID: 18042803] (ICC, Human)
• Cho CH, Parashurama N, Park EY, Suganuma K, Nahmias Y, Park J, Tilles AW, Berthiaume F, Yarmush ML Homogeneous differentiation of hepatocyte-like cells from embryonic stem cells: applications for the treatment of liver failure. FASEB J., 2007-10-17;22(3):898-909. 2007-10-17 [PMID: 17942827] (Flow Cytometry, ICC, Rat)
• Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. Proc. Natl. Acad. Sci. U.S.A., 2006-04-21;103(18):6907-12. 2006-04-21 [PMID: 16632596] (ICC, Human)

Bioinformatics

• Gene Symbol: FOXA2
• Uniprot:
  • Q9Y261()