HNF-3 beta/FoxA2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL ...read more
Mouse splenocytes were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. HNF‑3 beta /FoxA2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human HNF‑3 beta /FoxA2 ...read more
HNF‑3 beta /FoxA2 was detected in immersion fixed endoderm differentiated BG01V human embryonic stem cells using 10 µg/mL Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
HNF‑3 beta /FoxA2 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human HNF‑3 beta /FoxA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400) at 3 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ChIP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

HNF-3 beta/FoxA2 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human HNF‑3 beta /FoxA2
Met242-Ser457
Accession # Q9Y261
Specificity
Detects human HNF‑3 beta /FoxA2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXA2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Chromatin Immunoprecipitation (ChIP) 5 ug/5 x 10^6 cells
  • Immunocytochemistry 5-15 ug/mL
Control
HNF-3 beta/FoxA2 Knockout HeLa Cell Lysate
Publications
Read Publications using
AF2400 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HNF-3 beta/FoxA2 Antibody [Unconjugated]

  • forkhead box A2
  • Forkhead box protein A2
  • FoxA2
  • hepatic nuclear factor-3-beta
  • hepatocyte nuclear factor 3, beta
  • hepatocyte nuclear factor 3-beta
  • HNF3 beta
  • HNF-3 beta
  • HNF-3B
  • HNF-3-beta
  • HNF3BTCF-3B
  • MGC19807
  • TCF3B
  • Transcription factor 3B

Background

HNF-3 beta, also known as FoxA2, is a member of the forkhead class of DNA-binding proteins. It is a transcriptional activator for liver-specific transcripts such as albumin and transthyretin. Similar family members play roles in the differentiation of the pancreas and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for HNF-3 beta/FoxA2 Antibody (AF2400)(36)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: CyTof, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
CyTof
(1)
Flow Cytometry
(4)
ICC
(26)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(31)
Mouse
(4)
Rat
(1)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using AF2400 Applications Species
YY Kim, H Kim, CS Suh, HC Liu, Z Rosenwaks, SY Ku Effects of Natural Progesterone and Synthetic Progestin on Germ Layer Gene Expression in a Human Embryoid Body Model Int J Mol Sci, 2020;21(3):. 2020 [PMID: 31991577] (ICC, Human) ICC Human
M Riessland, B Kolisnyk, TW Kim, J Cheng, J Ni, JA Pearson, EJ Park, K Dam, D Acehan, LS Ramos-Espi, W Wang, J Zhang, JW Shim, G Ciceri, L Brichta, L Studer, P Greengard Loss of SATB1 Induces p21-Dependent Cellular Senescence in Post-mitotic Dopaminergic Neurons Cell Stem Cell, 2019;25(4):514-530.e8. 2019 [PMID: 31543366] (ICC, Human) ICC Human
K Lee, H Cho, RW Rickert, QV Li, J Pulecio, CS Leslie, D Huangfu FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation Cell Rep, 2019;28(2):382-393.e7. 2019 [PMID: 31291575] (Western Blot, Human) Western Blot Human
C Calatayud, G Carola, I Fernández-, M Valtorta, S Jiménez-De, M Díaz, J Soriano-Fr, G Cappellett, J García-San, Á Raya, A Consiglio CRISPR/Cas9-mediated generation of a tyrosine hydroxylase reporter iPSC line for live imaging and isolation of dopaminergic neurons Sci Rep, 2019;9(1):6811. 2019 [PMID: 31048719] (ICC, Human) ICC Human
T Zhou, TW Kim, CN Chong, L Tan, S Amin, Z Sadat Badi, S Mukherjee, Z Ghazizadeh, H Zeng, M Guo, M Crespo, T Zhang, R Kenyon, CL Robinson, E Apostolou, H Wang, JZ Xiang, T Evans, L Studer, S Chen A hPSC-based platform to discover gene-environment interactions that impact human ?-cell and dopamine neuron survival Nat Commun, 2018;9(1):4815. 2018 [PMID: 30446643] (ICC, Human) ICC Human
H Ida, T Akiyama, K Ishiguro, SK Goparaju, Y Nakatake, N Chikazawa-, S Sato, H Kimura, Y Yokoyama, M Nagino, MSH Ko, SBH Ko Establishment of a rapid and footprint-free protocol for differentiation of human embryonic stem cells into pancreatic endocrine cells with synthetic mRNAs encoding transcription factors Stem Cell Res Ther, 2018;9(1):277. 2018 [PMID: 30359326] (ICC, Human) ICC Human
E Senís, L Mosteiro, S Wilkening, E Wiedtke, A Nowrouzi, S Afzal, R Fronza, H Landerer, M Abad, D Niopek, M Schmidt, M Serrano, D Grimm AAVvector-mediated in vivo reprogramming into pluripotency Nat Commun, 2018;9(1):2651. 2018 [PMID: 29985406] (ICC, Mouse) ICC Mouse
B Kuebler, B Aran, L Miquel-Ser, Y Muñoz, E Ars, G Bullich, M Furlano, R Torra, M Marti, A Veiga, A Raya Generation of integration-free induced pluripotent stem cell lines derived from two patients with X-linked Alport syndrome (XLAS) Stem Cell Res, 2017;0(0):. 2017 [PMID: 29150092] (ICC, Human) ICC Human
L Miquel-Ser, A Duarri, Y Muñoz, B Kuebler, B Aran, C Costa, M Martí, M Comabella, S Malhotra, X Montalban, A Veiga, A Raya Generation of six multiple sclerosis patient-derived induced pluripotent stem cell lines Stem Cell Res, 2017;24(0):155-159. 2017 [PMID: 29034884] (ICC, Human) ICC Human
KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells Stem Cell Reports, 2017;0(0):. 2017 [PMID: 28602613] (ICC, Human) ICC Human
Show All 36 Publications.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 IC2400G
Biotin BAF2400

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Bioinformatics

Gene Symbol FOXA2
Uniprot