SOD1/Cu-Zn SOD Antibody - BSA Free

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Bovine ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.

Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of Superoxide Dismutase 1 in FFPE human liver tissue using an isotype control (top) and NBP2-24915 (bottom) at 5 ug/ml.

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Analysis of SOD in liver lysate of 1) human, 2) mouse, and 3) rat using this antibody. 025 ug/ml.

Western Blot: SOD1/Cu-Zn SOD Antibody [NBP2-24915] - Human ocular fluid. Antibody at 1:1000. Western blot image submitted by a verified customer review.

Production of superoxide radicals (O2–) and expression of antioxidant enzymes during MSC expansion, with or without methylene blue (MB) addition. a Quantification of superoxide radicals was carried out by MitoSOX staining (n = 3; values represent the mean +/- SEM). *p < 0.05. b Mitochondrial (MnSOD) and cytosolic superoxide dismutase (Cu/ZnSOD) Western blots were performed on proteins isolated from the same samples, and c results were normalized to actin Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/28061861), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

SOD1 does not affect laminin alpha 5 expression in endothelial cells. (A) mRNA levels of human SOD1 in 1G11-mock and 1G11-SOD1 cells, as determined by RT-qPCR. Each dot represents the mean of a triplicate from an independent experiment *** p < 0.001; two-tailed t-test. (B) Human SOD1 protein levels in 1G11-mock and 1G11-SOD1 cells as determined by immunoblotting; HEK-293T cells were used as a human SOD1 reference. Immunoblots were rehybridized with an anti-beta -actin antibody as a loading control. A representative experiment is shown. (C) 1G11-mock and 1G11-SOD1 cells were incubated (14 h) with the conditioned medium from N202.1A tumor cell line cultures (MCT) and then stained with DHE (red); nuclei were DAPI-counterstained (blue). Treatment with the SOD mimetic MnTBAP was used as control (right panels). Scale bars 25 um. (D) SOD activity in 1G11-mock and 1G11-SOD1 cell extracts. (E) Relative LAMA5 mRNA levels in 1G11-mock and 1G11-SOD1 cells. Each dot represents the mean of a triplicate from an independent experiment (n = 5). p = 0.32, two-tailed t-test. (F) Representative images of laminin alpha 5 (green; PAC078MV01) staining in 1G11-mock and 1G11-SOD1 cells; nuclei were DAPI counterstained (blue). Scale bars 50 um. (G) Quantification of laminin alpha 5 mean fluorescence intensity from the images as in (F) (n = 5 fields/condition). p = 0.58; two-tailed t-test. (H) Relative LAMA4 mRNA levels in 1G11-mock, 1G11-SOD3 and 1G11-SOD1 cells. Each dot represents the mean of a triplicate from an independent experiment (n = 6). ** p < 0.01, *** p < 0.001, two-tailed t-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35267534), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SOD1/Cu-Zn SOD Antibody - BSA Free Summary

• Immunogen: The superoxide dismutase enzyme from bovine erythrocytes (CAS # 9054-89-1) was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SOD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 - 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SOD1/Cu-Zn SOD Antibody - BSA Free

• ALS
• ALS1
• amyotrophic lateral sclerosis 1 (adult)
• Cu
• Cu/Zn superoxide dismutase
• CuZn SOD
• Cu-Zn SOD
• EC 1.15.1.1
• homodimer
• hSod1
• indophenoloxidase A
• Ipo1
• IPOA
• SOD
• SOD, cytosolic
• SOD, Soluble
• SOD1
• superoxide dismutase [Cu-Zn]
• Superoxide dismutase 1
• superoxide dismutase 1, soluble
• Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

FUNCTION: Destroys radicals which are normally produced within the cells and which are toxic to biological systems. CATALYTIC ACTIVITY: 2 superoxide + 2 H+ = O2 + H2O2. COFACTOR: Binds 1 copper ion per subunit. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: Defects in SOD1 are the cause of familial amyotrophic lateral sclerosis (FALS); also called amyotrophic lateral sclerosis 1 (ALS1 or ALS). ALS is a degenerative disorder of motorneurons in the cortex, brainstem and spinal cord. ALS is characterized by muscular weakness and atrophy beginning in the hands and spreading to the forearms and legs. Muscle fasciculations are commonly visible. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. ALS is sometimes referred to as Lou Gehrig disease after the famous American baseball player who was diagnosed with the disorder. FALS, the familial form of ALS, accounts for about 10% of the cases and is transmitted in an autosomal dominant manner. The mean age at onset of FALS is 45 years. MISCELLANEOUS: Zinc binding promotes dimerization. SIMILARITY: Belongs to the Cu-Zn superoxide dismutase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOD1/Cu-Zn SOD Antibody (NBP2-24915)(21)

Publications using NBP2-24915

• Mueller, J;van Muilekom, DR;Ehlers, J;Suhr, M;Hornburg, SC;Bang, C;Wilkes, M;Schulthei beta , T;Maser, E;Rebl, A;Goldammer, T;Seibel, H;Schulz, C; Dietary Chlorella vulgaris supplementation modulates health, microbiota and the response to oxidative stress of Atlantic salmon Scientific reports 2024-10-10 [PMID: 39389986]
• Noor MI, Rahman MS Roundup® disrupts tissue architecture, attenuates Na+/K+-ATPase expression, and induces protein oxidation/nitration, cellular apoptosis, and antioxidant enzyme expressions in the gills of goldfish, Carassius auratus Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 2023-07-31 [PMID: 37532112]
• Martinez-Martel I, Bai X, Batalle G, Pol O New Treatment for the Cognitive and Emotional Deficits Linked with Paclitaxel-Induced Peripheral Neuropathy in Mice Antioxidants (Basel, Switzerland) 2022-12-01 [PMID: 36552595] (WB, Mouse)
• Najjar R Raspberry Polyphenols Target Molecular Pathways of Heart Failure Thesis 2023-01-01
• Chang YJ, Jenny LA, Li YS et al. CRISPR editing demonstrates rs10490924 raised oxidative stress in iPSC-derived retinal cells from patients with ARMS2/HTRA1-related AMD Proceedings of the National Academy of Sciences of the United States of America 2023-05-09 [PMID: 37126685]
• F Wei, CJ Neal, TS Sakthivel, Y Fu, M Omer, A Adhikary, S Ward, KM Ta, S Moxon, M Molinari, J Asiatico, M Kinzel, SN Yarmolenko, V San Cheong, N Orlovskaya, R Ghosh, S Seal, M Coathup A novel approach for the prevention of ionizing radiation-induced bone loss using a designer multifunctional cerium oxide nanozyme Bioactive materials, 2022-09-21;21(0):547-565. 2022-09-21 [PMID: 36185749] (Western Blot, Rat)
• Lacy B, Rahman MS Interactive effects of high temperature and pesticide exposure on oxidative status, apoptosis, and renin expression in kidney of goldfish: Molecular and cellular mechanisms of widespread kidney damage and renin attenuation Journal of applied toxicology : JAT 2022-06-13 [PMID: 35698815]
• Lacy B, Rahman MS, Rahman MS Potential mechanisms of Na+/K+-ATPase attenuation by heat and pesticides co-exposure in goldfish: role of cellular apoptosis, oxidative/nitrative stress, and antioxidants in gills Environmental science and pollution research international [PMID: 35352221]
• Pouso-Vazquez E, Bai X, Batalle G et al. Effects of heme oxygenase 1 in the molecular changes and neuropathy associated with type 2 diabetes in mice Biochemical pharmacology 2022-03-08 [PMID: 35276215] (WB, Mouse)
• Carmona-Rodriguez L, Martinez-Rey D, Martin-Gonzalez P et al. Superoxide Dismutase-3 Downregulates Laminin alpha5 Expression in Tumor Endothelial Cells via the Inhibition of Nuclear Factor Kappa B Signaling Cancers 2022-02-26 [PMID: 35267534] (WB, Human)
• Pek E, Canbey Goret C, Hacivelioglu S et al. The immunohistochemical and histologic effects of contrast medium on uterus, fallopian tubes and ovaries, given during hysterosalpingography: rat study Journal of the Turkish German Gynecological Association 2020-12-04 [PMID: 32500677] (IHC-P, Rat)
• Kwon I, Song W, Jang Y et al. Elevation of hepatic autophagy and antioxidative capacity by endurance exercise is associated with suppression of apoptosis in mice Annals of Hepatology [PMID: 31611063]
• Han J, Kim S, Ryu M et al. Chloramphenicol Mitigates Oxidative Stress by Inhibiting Translation of Mitochondrial Complex I in Dopaminergic Neurons of Toxin-Induced Parkinson s Disease Model Oxidative Medicine and Cellular Longevity 2019-08-26 [PMID: 31534621] (WB, Mouse)
• Mishra P, Paital B, Jena S et al. Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone induced oxidative stress via NF kappa B/AKT/mTOR/KEAP1 signalling in rat heart Sci Rep 2019-05-15 [PMID: 31092832] (WB, Rat)
• Ghaffari, R;Di Bona, KR;Riley, CL;Richburg, JH; Copper transporter 1 (CTR1) expression by mouse testicular germ cells, but not Sertoli cells, is essential for functional spermatogenesis PLoS ONE 2019-04-19 [PMID: 31002737] (WB, Mouse)
• Millett CE, Phillips BE, Saunders EFH. The Sex-specific Effects of LPS on Depressive-like Behavior and Oxidative Stress in the Hippocampus of the Mouse Neuroscience 2018-12-14 [PMID: 30553795] (WB, Mouse)
• De Riccardis L, Buccolieri A, Muci M et al. Copper and ceruloplasmin dyshomeostasis in serum and cerebrospinal fluid of multiple sclerosis subjects. Biochim. Biophys. Acta. 2018-03-08 [PMID: 29524632] (WB, Human)
• Yao Y, Wang Y, Zhang Y, Liu C. Klotho ameliorates oxidized low density lipoprotein (ox-LDL)-induced oxidative stress via regulating LOX-1 and PI3K/Akt/eNOS pathways. Lipids Health Dis. 2017-04-13 [PMID: 28407763] (WB, Human)
• Bertolo A, Capossela S, Frankl G et al. Oxidative status predicts quality in human mesenchymal stem cells Stem Cell Res Ther Jan 6 2017 [PMID: 28061861] (FLOW, Human). Stem Cell Res Ther. 2017-01-06 [PMID: 28061861] (Western Blot, Rat)
  
  Details:
  This citation used the FITC version of this antibody.
• Sagar S, Kumar P, Behera RR, Pal A. Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells. J. Hazard. Mater. 2014-06-06 [PMID: 24976129] (WB, Mouse)
  
  Details:
  Fig 6: RAW264.7
• Kumar P, Rao GN, Pal BB, Pal A. Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells. Int. J. Biochem. Cell Biol. 2014-06-04 [PMID: 24907397] (WB, Rat)
  
  Details:
  Fig 2B: primary astroglial cells. Catalase was detected below the 72 kDa marker.

Reviews for SOD1/Cu-Zn SOD Antibody (NBP2-24915) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Bovine.

reviewed by: Verified Customer WB Bovine 09/23/2020

Summary

• Application: Western Blot
• Sample Tested: ocular fluid
• Species: Bovine
• Lot: 03253462c2b-3

Comments

• Comments: use at a dilution of 1:1000

reviewed by: Verified Customer WB Human 09/23/2020

Summary

• Application: Western Blot
• Sample Tested: ocular fluid
• Species: Human
• Lot: 03253462c2b-3

Comments

• Comments: use at dilution 1:1000

reviewed by: Verified Customer WB Mouse 01/12/2017

Summary

• Application: Western Blot
• Sample Tested: Liver
• Species: Mouse
• Lot: 03253462C2B

Comments

• Comments: N/A

Bioinformatics

• Gene Symbol: SOD1
• Uniprot:
  • P00441()