Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SNX27 on mouse brain extracts.
Immunocytochemistry/ Immunofluorescence: SNX27 Antibody [NBP1-45283] - Detection of SNX27 (Green) in Hela cells using NBP1-45283. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: SNX27 Antibody [NBP1-45283] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.
Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SXN27 antibody in A. 293T + tagged SNX27 protein and B. 293T empty vector.
Simple Western: SNX27 Antibody [NBP1-45283] - Simple Western lane view shows a specific band for SNX27 in 0.5 mg/ml of Human Cerebellum (left) and Mouse Cerebellum (right) lysate. This experiment was performed under ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, 30% glycerol
0.1% Sodium Azide
Immunogen affinity purified
This SNX27 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin embedded sections and Western blot, where a band is seen at ~65 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Human and mouse. Predicted to react with rat, bovine and Zebrafish based on 100% sequence homology.
Alternate Names for SNX27 Antibody
methamphetamine-responsive transcript 1
sorting nexin family member 27
SNX27 belongs to SNX family of proteins which are unified by the presence of a phox domain, a phospholipid-binding motif, and are involved in intracellular sorting as well as trafficking. SNX27 was initially discovered in neocortex of rat as a developmentally regulated psychostimulant-inducible novel gene, which was named mrt1 (methamphetamine-responsive transcript 1), having two splice forms mrt1a (SNX27a) and mrt1b (SNX27b) that vary only in their C-terminal ends. In SNX family, SNX27 is the only SNX member containing a PDZ domain and it interact with the PDZ-bm of a few membrane proteins through its PDZ domain to regulate their intracellular sorting. SNX27 modulates the early endosomal trafficking of 5-HT4(a)R [5-hydroxytryptamine type-4(a) receptor], Kir3 (G protein-gated potassium) channels, NR2C (N-methyl-D-aspartate receptor 2C) etc., and has also been suggested to facilitate efficient recycling of beta2AR/beta2-adrenoreceptor to the plasma membranes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Spotlight on SNX27 Antibodies Sorting Nexin Family Member 27 (SNX27) binds the cell membrane to regulate endocytic vesicular and protein trafficking. SNX27 has also been shown to play a key role in the endocytic recycling pathway, whereby internalized membrane proteins and lipids... Read full blog post.