SNX27 Antibody

Images

 
Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SNX27 on mouse brain extracts.
Immunocytochemistry/ Immunofluorescence: SNX27 Antibody [NBP1-45283] - Detection of SNX27 (Green) in Hela cells using NBP1-45283. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: SNX27 Antibody [NBP1-45283] - IHC analysis of SNX27 in mouse bladder using DAB with hematoxylin counterstain.
Western Blot: SNX27 Antibody [NBP1-45283] - Analysis of SXN27 antibody in A. 293T + tagged SNX27 protein and B. 293T empty vector.
Simple Western: SNX27 Antibody [NBP1-45283] - Simple Western lane view shows a specific band for SNX27 in 0.5 mg/ml of Human Cerebellum (left) and Mouse Cerebellum (right) lysate. This experiment was performed under ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ZeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

SNX27 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of human SNX27 (within residues 450-500). [Swiss-Prot# Q96L92]
Localization
Cytoplasm cytosol. Early endosome. Note: In T lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products.
Predicted Species
Rat (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNX27
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western 10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This SNX27 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin embedded sections and Western blot, where a band is seen at approx. 65 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
RAIDD Antibody (NB100-66308)
SMARCD3 Antibody (H00006604-M01)
Control Peptide
SNX27 Peptide (NBP1-45283PEP)
Publications
Read Publication using
NBP1-45283 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse. Predicted to react with rat, bovine and Zebrafish based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SNX27 Antibody

  • KIAA0488MGC126873
  • methamphetamine-responsive transcript 1
  • MGC126871
  • MGC20471
  • MRT1
  • MY014
  • sorting nexin family member 27
  • sorting nexin-27

Background

SNX27 belongs to SNX family of proteins which are unified by the presence of a phox domain, a phospholipid-binding motif, and are involved in intracellular sorting as well as trafficking. SNX27 was initially discovered in neocortex of rat as a developmentally regulated psychostimulant-inducible novel gene, which was named mrt1 (methamphetamine-responsive transcript 1), having two splice forms mrt1a (SNX27a) and mrt1b (SNX27b) that vary only in their C-terminal ends. In SNX family, SNX27 is the only SNX member containing a PDZ domain and it interact with the PDZ-bm of a few membrane proteins through its PDZ domain to regulate their intracellular sorting. SNX27 modulates the early endosomal trafficking of 5-HT4(a)R [5-hydroxytryptamine type-4(a) receptor], Kir3 (G protein-gated potassium) channels, NR2C (N-methyl-D-aspartate receptor 2C) etc., and has also been suggested to facilitate efficient recycling of beta2AR/beta2-adrenoreceptor to the plasma membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SNX27 Antibody (NBP1-45283)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

View specific protocols for SNX27 Antibody (NBP1-45283): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SNX27 Antibody (NBP1-45283) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-45283AF405
Alexa Fluor 488 Labeled NBP1-45283AF488
Alexa Fluor 647 Labeled NBP1-45283AF647
Alexa Fluor 700 Labeled NBP1-45283AF700
Biotin Labeled NBP1-45283B
DyLight 405 Labeled NBP1-45283V
DyLight 488 Labeled NBP1-45283G
DyLight 550 Labeled NBP1-45283R
DyLight 650 Labeled NBP1-45283C
FITC Labeled NBP1-45283F
HRP Labeled NBP1-45283H

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SNX27 NBP1-45283

Bioinformatics Tool for SNX27 Antibody (NBP1-45283)

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Blogs on SNX27.

Spotlight on SNX27 Antibodies
Sorting Nexin Family Member 27 (SNX27) binds the cell membrane to regulate endocytic vesicular and protein trafficking. SNX27 has also been shown to play a key role in the endocytic recycling pathway, whereby internalized membrane proteins and lipids...  Read full blog post.

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Bioinformatics

Gene Symbol SNX27
Entrez
Uniprot