DCP1B Antibody - BSA Free

Western Blot: DCP1B Antibody [NBP1-82018] - Analysis in human cell line NTERA-2.

Immunocytochemistry/Immunofluorescence: DCP1B Antibody [NBP1-82018] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: DCP1B Antibody [NBP1-82018] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DCP1B Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QYEQLKAHQGTGAGISPVILNSGEGKEVDILRMLIKAKDEYTKCKTCSEPKKITSSSAIYDNPNLIKPIPVKPSENQQQRIPQPNQTLDPEPQHLSLTALFGKQD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DCP1B
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: WB, ICC/IF reported in scientific literature (PMID: 27918561). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  DCP1B Protein (NBP1-82018PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DCP1B Antibody - BSA Free

• DCP1 decapping enzyme homolog B (S. cerevisiae)
• DCP1
• decapping enzyme hDcp1b
• EC 3.-
• FLJ31638
• hDcp1b
• mRNA-decapping enzyme 1B

Background

DCP1B is a core component of the mRNA decapping complex, a key factor in the regulation of mRNA decay (Lykke-Andersen,2002 (PubMed 12417715)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DCP1B Antibody (NBP1-82018)(2)

Publications using NBP1-82018

• Chen TW, Liao HW, Noble M et al. Human DCP1 is crucial for mRNA decapping and possesses paralog-specific gene regulating functions eLife 2024-11-01 [PMID: 39485278]
• D'Lima NG, Ma J, Winkler L et al. A human microprotein that interacts with the mRNA decapping complex. Nat Chem Biol 2017-02-01 [PMID: 27918561] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: DCP1B