MRP4/ABCC4 Antibody

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Immunohistochemistry-Paraffin: MRP4/ABCC4 Antibody [NB100-1471] - (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MRP4/ABCC4 Antibody Summary

Immunogen
Peptide with sequence CNGQPSTLTIFETAL corresponding to C-Terminus according to NP_005836.2.
Localization
Cytoplasm
Specificity
This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ABCC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 2-4 ug/ml
  • Immunohistochemistry-Paraffin 2-4 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
ICC/IF successful usage for human primary hepatocytes is reported in scientific literature (PMID: 20395535). Data obtained from a previous batch (different goat). WB: Approx. 170 kDa band observed in rat renal cortex membranes (calculated MW of 149 kDa band according to rat NP_596902.1 and 150 kDa band according to human NP_005836.2) is provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Data obtained from a previous batch (different goat). IHC-P: Human prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium.
Publications
Read Publications using
NB100-1471 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28785115).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MRP4/ABCC4 Antibody

  • ABCC4
  • ATP-binding cassette sub-family C member 4
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 4
  • EST170205
  • MOATB
  • MOAT-BbA464I2.1 (ATP-binding cassette, sub-family C (CFTR/MRP), member 4)
  • MOATBcanalicular multispecific organic anion transporter (ABC superfamily)
  • MRP4
  • MRP4MRP/cMOAT-related ABC transporter
  • multidrug resistance-associated protein 4
  • multispecific organic anion transporter B
  • Multi-specific organic anion transporter B

Background

MRP4 may be an organic anion pump relevant to cellular detoxification

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MRP4/ABCC4 Antibody (NB100-1471)(8)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(1)
WB
(4)
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Human
(1)
Mouse
(1)
Rat
(2)
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Showing Publications 1 - 8 of 8.
Publications using NB100-1471 Applications Species
Blazquez AMG, Macias RIR, Cives-Losada C et al. Lactation during cholestasis: Role of ABC proteins in bile acid traffic across the mammary gland. Sci Rep 2017 Aug 07 [PMID: 28785115] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Domingue JC, Ao M, Sarathy J et al. HEK-293 cells expressing the cystic fibrosis transmembrane conductance regulator (CFTR): a model for studying regulation of Cl- transport. Physiol Rep. 2014 Sep 01 [PMID: 25263207] (WB, Human)

Details:
MRP4 antibody used for WB at 1:750 dilution on cytosolic and total lysate fractions of HEK?CFTR cells as well as T84 cells (positive control). The antibody detected a band of 170 kDa size and the data is shown in Figure 5A - lower panel.
WB Human
Nomura M, Motohashi H, Sekine H et al. Developmental expression of renal organic anion transporters in rat kidney and its effect on renal secretion of phenolsulfonphthalein Am J Physiol Renal Physiol 2012 Jun [PMID: 22419695] (WB, Rat) WB Rat
Naud J, Laurin L-P, Michaud J et al. Effects of Chronic Renal Failure on Brain Drug Transporters in Rats. Drug Metabolism and Disposition: The Biological Fate of Chemicals. 2011 Oct 3. [PMID: 21969519]
Naud J, Michaud J, Beauchemin S et al. Effects of chronic renal failure on kidney drug transporters and cytochrome P450 in rats. Drug Metab Dispos;39(8):1363-9. 2011 Aug. [PMID: 21525170]
Xu S, Weerachayaphorn J, Cai SY et al. Aryl hydrocarbon receptor and NF-E2-related factor 2 are key regulators of human MRP4 expression. Am J Physiol Gastrointest Liver Physiol 2010 Jul [PMID: 20395535]
Cheng D, Ren J, Jackson EK. Multidrug resistance protein 4 mediates cAMP efflux from rat preglomerular vascular smooth muscle cells. Clin Exp Pharmacol Physiol 2010 Feb [PMID: 19671067] (WB, Rat) WB Rat
Schuetz JD, Connelly MC, Sun D et al. MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs. Nat Med 1999 Sep [PMID: 10470083]

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ABCC4
Entrez
Uniprot