CYTIP Antibody

Images

 
Western Blot: CYTIP Antibody [NB100-1002] - Staining of Jurkat (A) and (1 ug/mL) MOLT4 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.5 ug/mL. Detected by chemiluminescence.
Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ...read more
Immunofluorescence: CYTIP Antibody [NB100-1002] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ...read more

Product Details

Summary
Reactivity Hu, Po, CaSpecies Glossary
Applications WB, PEP-ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CYTIP Antibody Summary

Immunogen
Peptide with sequence SLQRLLQHSSNGN-C corresponding to N-Terminus according to NP_004279.3.
Predicted Species
Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CYTIP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/mL
Application Notes
Western blot: Approx 38 kDa band observed in lysates of cell line Jurkat and approx 40 kDa in lysates of cell line MOLT4 (calculated MW of 40 kDa according to NP_004279.3).
Control
Jurkat Whole Cell Lysate
CYTIP Overexpression Lysate
Publications
Read Publication using NB100-1002.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CYTIP Antibody

  • B3-1
  • CASPCytohesin-associated scaffolding protein
  • Cbp HE
  • CYBRCytohesin-binding protein HE
  • CYTHIP
  • cytohesin 1 interacting protein
  • Cytohesin binder and regulator
  • cytohesin-1 interacting protein
  • cytohesin-interacting protein
  • HEPleckstrin homology Sec7 and coiled-coil domains-binding protein
  • pleckstrin homology, Sec7 and coiled/coil domains, binding protein
  • pleckstrin homology, Sec7 and coiled-coil domains, binding protein
  • PSCDBPcytohesin binding protein HE

Background

This protein contains 2 leucine zipper domains and a putative C-terminal nuclear targeting signal, and it does not have any hydrophobic regions. It is expressed weakly in resting NK and T cells. It and cytohesin-1 physically interact through their coiled-coil domains. Cytohesin-1, in addition to its role in cell adhesion, is a guanine nucleotide-exchange protein activator of ARF GTPases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CYTIP Antibody (NB100-1002)(1)

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CYTIP
Entrez
Uniprot