Ezrin Antibody

Western Blot: Ezrin Antibody [NBP2-16396] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunocytochemistry/Immunofluorescence: Ezrin Antibody [NBP2-16396] - HCT116 cells.

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - C2C12 xenograft, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Human colon carcinoma, using Ezrin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

IHC-Whole-Mount- Ezrin Antibody [NBP2-16396]--Fixed zebrafish embryos. Ezrin antibody [N2C2], Internal dilution: 1:200.

IHC-Whole-Mount- Ezrin Antibody [NBP2-16396] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: Ezrin stained by Ezrin antibody [N2C2], Internal diluted at 1:100. Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70C

Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody

Western Blot: Ezrin Antibody [NBP2-16396] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Ezrin antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Ze, Bv, Ca, Ch, Pm, Rb
• Applications: WB, ICC/IF, IHC, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ezrin Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary.
• Localization: Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; microvillus membrane; ruffle membrane; Cytoplasm; cell cortex
• Predicted Species: Bovine (96%), Rabbit (94%), Chimpanzee (99%), Chicken (92%), Canine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EZR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry Whole-Mount Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Adrenal Membrane Tissue Lysate (Adult Membrane Normal)

Reactivity Notes

Xenopus laevis (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS pH7, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Ezrin Antibody

• CVIL
• CVL
• Cytovillin 2
• Cytovillin
• DKFZp762H157
• EZR
• Ezrin
• FLJ26216
• p81
• VIL2
• VIL2MGC1584
• villin 2 (ezrin)
• Villin 2
• villin-2

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Ezrin Antibody (NBP2-16396)(3)

Publications using NBP2-16396

• Tang Y, Sun X, et al. Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma. Oncol Lett 2019-07-01 [PMID: 31289527] (IF/IHC, Human)
• Ye, S;Xu, Y;Li, J;Zheng, S;Sun, P;Wang, T; Prognostic role of GPER/Ezrin in triple negative breast cancer is associated with menopausal status Endocr Connect 2019-04-01 [PMID: 30999280] (IHC-P, Human)
• Law CH, Li JM, Chou HC et al. Hyaluronic acid-dependent protection in H9C2 cardiomyocytes: A cell model of heart ischemia-reperfusion injury and treatment. Toxicology 2013-01-01 [PMID: 23178681] (WB, Rat)

Review for Ezrin Antibody (NBP2-16396) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 10/16/2015

Summary

• Application: Western Blot
• Sample Tested: Hela whole cell lysate
• Species: Human
• Lot: 40660

Bioinformatics

• Gene Symbol: EZR
• Uniprot:
  • P15311(Human)