Ezrin Antibody

Images

 
Western Blot: Ezrin Antibody [NBP2-16396] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP2-16396] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ezrin protein stained by Ezrin antibody [N2C2], Internal diluted at 1:1000. ...read more
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - araffin-embedded rat duodenum. Ezrin antibody [N2C2], Internal diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Ezrin Antibody [NBP2-16396] - HCT116 cells.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Ezrin Antibody [NBP2-16396] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Ezrin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Ezrin. The exact sequence is proprietary.
Localization
Apical cell membrane; Peripheral membrane protein; Cytoplasmic side; Cell projection; microvillus membrane; ruffle membrane; Cytoplasm; cell cortex
Predicted Species
Bovine (96%), Rabbit (94%), Primate (99%), Chicken (92%), Canine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EZR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Adrenal Lysate (NB820-60567)
Reviewed Applications
Read 1 Review rated 5
using
NBP2-16396 in the following applications:

Publications
Read Publication using
NBP2-16396 in the following applications:

  • WB
    1 publication

Reactivity Notes

Chimpanzee (99%), Xenopus laevis (85%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ezrin Antibody

  • CVIL
  • CVL
  • Cytovillin 2
  • Cytovillin
  • DKFZp762H157
  • EZR
  • Ezrin
  • FLJ26216
  • p81
  • VIL2
  • VIL2MGC1584
  • villin 2 (ezrin)
  • Villin 2
  • villin-2

Background

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ezrin Antibody (NBP2-16396)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Ezrin Antibody (NBP2-16396) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-16396:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Ezrin NBP2-16396
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reviewed by:
Anonymous
WB Human 10/16/2015
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman
Lot40660

Blocking

Blocking DetailsBlocker=Skimmed milk, Buffer= 1XTris Buffer Saline+0.1 % Tween 20, 2hrs at RT

Primary Anitbody

Dilution Ratio1:500 over night at 4 degree Celsius

Secondary Antibody

Secondary Descriptionanti-rabbit IgG, HRP
Secondary Manufacturer Cat#7074P2
Secondary Concentration1:20,000

Details

Detection Notes60 seconds exposure

Comments

CommentsIt works nicely.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ezrin Antibody (NBP2-16396) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Ezrin Products

Bioinformatics Tool for Ezrin Antibody (NBP2-16396)

Discover related pathways, diseases and genes to Ezrin Antibody (NBP2-16396). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Ezrin Antibody (NBP2-16396)

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Pathways for Ezrin Antibody (NBP2-16396)

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Blogs on Ezrin

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Recent Reviews

5
5
1
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1
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Anonymous
10/16/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol EZR
Entrez
Uniprot