Kir2.1 Antibody (S112)

Western Blot: Kir2.1 Antibody (S112) [NBP2-12900] - Western Blot analysis of Monkey COS transient cell lysate showing detection of Kir2.1 Potassium Channel protein using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence: Kir2.1 Antibody (S112) [NBP2-12900] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kir2.1 Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kir2.1 Monoclonal Antibody (NBP2-12900) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kir2.1 Antibody (D) Composite.

Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Immunohistochemistry analysis using Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody, Clone S112 (NBP2-12900). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody (NBP2-12900) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

Immunohistochemistry: Kir2.1 Antibody (S112) [NBP2-12900] - Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kir2.1 Potassium Channel Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear expression in the epidermis and hair follicles.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, Simple Western, ICC/IF, IHC, MA
• Clone: S112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Kir2.1 Antibody (S112) Summary

• Immunogen: Fusion protein amino acids 41-64 and 189-428 of mouse Kir2.1
• Localization: Membrane
• Specificity: Detects approx 45 kDa. No cross-reactivity against Kir2.2 or Kir2.3.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KCNJ2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin
  • Microarray
  • Simple Western 1:100
  • Western Blot 1:1000
• Application Notes: 1 ug/mL of Kir2.1 Antibody was sufficient for detection of Kir2.1 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. This Kir2.1 Antibody (S112B-14) is validated for Simple Western from a verified customer review. Simple Western reported by an internal validation. Separated by Size, antibody dilution of 1:100

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Kir2.1 Antibody (S112)

• ATFB9
• Cardiac inward rectifier potassium channel
• HHBIRK1
• HHIRK1
• HIRK1
• Inward rectifier K(+) channel Kir2.1
• inward rectifier K+ channel KIR2.1
• inward rectifier potassium channel 2
• IRK1
• IRK-1
• IRK1LQT7
• KCNJ2
• Kir2.1
• LQT7
• Potassium channel, inwardly rectifying subfamily J member 2
• potassium inwardly-rectifying channel, subfamily J, member 2
• SQT3

Background

1 ug/mL was sufficient for detection of Kir2.1 in 10ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Kir2.1 Antibody (NBP2-12900)(1)

Publication using NBP2-12900

• Klose E, Kuhrt H, Kohen L Et al. Hypoxic and osmotic expression of Kir2.1 potassium channels in retinal pigment epithelial cells: Contribution to vascular endothelial growth factor expression Experimental eye research 2021-08-21 [PMID: 34425102] (WB, Human, Rat)

Review for Kir2.1 Antibody (NBP2-12900) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: auriane ernault Simple Western Rat 02/17/2021

Summary

• Application: Simple Western
• Sample Tested: primary neonatal ventricular cardiomyocytes
• Species: Rat
• Lot: 1205

Comments

• Comments: ***Novus Innovators Program - new species or application used on a primary antibody.

Bioinformatics

• Gene Symbol: KCNJ2