ARC/NOL3 Antibody

Images

 
Western Blot: ARC/NOL3 Antibody [NBP2-19561] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: ARC/NOL3 Antibody [NBP2-19561] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using NOL3 antibody (Green) at 1:500 dilution. Alpha-tubulin ...read more
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Mouse brain. NOL3 stained by NOL3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - Hela xenograft, using NOL3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NOL3 stained by NOL3 ...read more
Immunohistochemistry-Frozen: ARC/NOL3 Antibody [NBP2-19561] - NOL3 antibody detects NOL3 protein by immunohistochemical analysis.Sample: Frozen-sectioned mouse cerebellum. Green: NOL3 stained by NOL3 antibody diluted at ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

ARC/NOL3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NOL3. The exact sequence is proprietary.
Localization
Isoform 1: Nucleus; nucleolus; Isoform 2: Cytoplasm
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOL3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for ARC/NOL3 Antibody

  • Apoptosis repressor with CARD
  • ARC
  • ARCFLJ35304
  • CARD2
  • Muscle-enriched cytoplasmic protein
  • MYC
  • MYP
  • NOL3
  • NOP30
  • NOPnucleolar protein 3
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • Nucleolar protein of 30 kDa

Background

This gene encodes an anti-apoptotic protein that has been shown to down-regulate the enzyme activities of caspase 2, caspase 8 and tumor protein p53. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ARC/NOL3 Antibody (NBP2-19561)

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Bioinformatics

Gene Symbol NOL3