SMAD6 Antibody

Images

 
Western Blot: SMAD6 Antibody [NB100-56440] - analysis using SMAD6 antibody. Lysate from Jurkat cells probed with SMAD6 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Simple Western: SMAD6 Antibody [NB100-56440] - Simple Western lane view shows a specific band for SMAD6 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SMAD6 Antibody Summary

Immunogen
This antibody was developed against 2 synthetic peptides found between amino acids 30-150 and 300-400 of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6 in multiple species.
Specificity
A 52 kDa band is observed.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD6
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Simple Western 1:50
  • Immunocytochemistry/Immunofluorescence 1-5 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
Application Notes
Use of this antibody in IHC-P and ICC-IF applications is cited by Hogg et al 2010 in Endocrinology 151:1247-56 (PMID: 20032053) and this publication has documented protocols for these applications.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Jurkat Lysate (NB800-PC2)
NIH 3T3 Lysate (NB800-PC8)
Publications
Read Publications using
NB100-56440 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    3 publications

Reactivity Notes

Cross reacts with New World Monkey. Predicted to react with Horse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for SMAD6 Antibody

  • hSMAD6
  • HsT17432
  • MAD homolog 6
  • MADH6SMAD, mothers against DPP homolog 6 (Drosophila)
  • MADH7
  • mothers against decapentaplegic homolog 6
  • Mothers against decapentaplegic, drosophila, homolog of, 6
  • Mothers against DPP homolog 6
  • SMAD 6
  • SMAD family member 6MAD, mothers against decapentaplegic homolog 6 (Drosophila)
  • SMAD, mothers against DPP homolog 6
  • Smad6

Background

SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMAD6 Antibody (NB100-56440)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Sheep.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Sheep
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-56440 Applications Species
Childs AJ, Kinnell HL, Collins CS et al. BMP signaling in the human fetal ovary is developmentally regulated and promotes primordial germ cell apoptosis. Stem Cells. 2010 Aug [PMID: 20506112] (IHC-P, Human)

Details:
SMAD6 (IMG-555). IHC (Bouin's fixed, paraffin-embedded): Human fetal ovary, Fig 3A-3D.
IHC-P Human
Hogg K, Etherington SL, Young JM et al. Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family. Endocrinology. 2010 Mar [PMID: 20032053] (IHC, Sheep)

Details:
Antibodies cited (sheep ovary): 1. SMAD6 (IMG-555): IHC (Bouin's-fixed, paraffin-embedded), Fig 3G,H; IF, Fig 4B. 2. SMAD7 (IMG-531): IHC (Bouin's-fixed, paraffin-embedded), Fig 3J,K. Note: See Table 1 for antibody dilutions used.
IHC Sheep
Yoshiura S, Ohtsuka T, Takenaka Y et al. Ultradian oscillations of Stat, Smad, and Hes1 expression in response to serum. Proc Natl Acad Sci U S A. 2007 Jul 3 [PMID: 17592117] (WB, Mouse)

Details:
WB, Fig 3 (mouse C3HIOT1/2 fibroblasts).
WB Mouse
Wang Q, Wei X, Zhu T et al. Bone morphogenetic protein 2 activates Smad6 gene transcription through bone-specific transcription factor Runx2. J Biol Chem. 2007 Apr 6 [PMID: 17215250] (WB, Mouse)

Details:
WB, Fig. 1 (2C12 mouse myoblast/osteoblast cells).
WB Mouse
Yamaguchi H, Zhu J, Yu T et al. Low-level bisphenol A increases production of glial fibrillary acidic protein in differentiating astrocyte progenitor cells through excessive STAT3 and Smad1 activation. Toxicology. 2006 Sep 21 [PMID: 16860915]
Shen R, Chen M, Wang YJ et al. Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation. J Biol Chem. 2006 Feb 10 [PMID: 16299379] (WB)

Details:
[WB, Fig 2f (COS monkey kidney cells, 2C12 mouse myoblast/osteoblast cells), WB, Fig 5a (Specificity of antibody validated by shRNA knockdown in 2T3 cells)]
WB

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SMAD6
Entrez
Uniprot