Western Blot: SMAD6 Antibody [NB100-56440] - analysis using SMAD6 antibody. Lysate from Jurkat cells probed with SMAD6 antibody at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: SMAD6 Antibody [NB100-56440] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Simple Western: SMAD6 Antibody [NB100-56440] - Simple Western lane view shows a specific band for SMAD6 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
This antibody was developed against 2 synthetic peptides found between amino acids 30-150 and 300-400 of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6 in multiple species.
A 52 kDa band is observed.
Protein G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Western Blot 0.5-2 ug/ml
Simple Western 1:50
Immunocytochemistry/Immunofluorescence 1-5 ug/ml
Immunohistochemistry 5 - 10 ug/ml
Immunohistochemistry-Paraffin 5-10 ug/ml
Use of this antibody in IHC-P and ICC-IF applications is cited by Hogg et al 2010 in Endocrinology 151:1247-56 (PMID: 20032053) and this publication has documented protocols for these applications. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Read Publications using NB100-56440 in the following applications:
Cross reacts with New World Monkey. Predicted to react with Horse.
Alternate Names for SMAD6 Antibody
MAD homolog 6
MADH6SMAD, mothers against DPP homolog 6 (Drosophila)
mothers against decapentaplegic homolog 6
Mothers against decapentaplegic, drosophila, homolog of, 6
Mothers against DPP homolog 6
SMAD family member 6MAD, mothers against decapentaplegic homolog 6 (Drosophila)
SMAD, mothers against DPP homolog 6
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, SMAD2, SMAD5, SMAD8 and SMAD9), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and SMAD7). SMAD1, SMAD5, SMAD8 and SMAD9 have high degrees of homology and antibodies are available that recognize sequences common to all of them. SMAD8 and SMAD9 are typically used as alternate names for one another in the literature. Human SMAD1 is a 465 amino acid protein; GenBank Accession No. AAP36050.1. Human SMAD2 is a 467 amino acid protein; GenBank Accession No. AAC51918.1 Human SMAD3 is a 425 amino acid protein; GenBank Accession No. NP_005893.1 Human SMAD4 is a 552 amino acid protein GenBank Accession No. NP_005350.1. Human SMAD5 is a 465 amino acid protein; GenBank Accession No. AAC50791.1. Human SMAD6 is a 496 amino acid protein; GenBank Accession No. AAH12986.1 Human SMAD7 is a 426 amino acid protein; GenBank Accession No. AAB81354.1 Mouse SMAD8 is a 430 amino acid protein; GenBank Accession No. AAN85445.1 Human SMAD9 is a 430 amino acid protein; GenBank Accession No. NP_005896.1
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.