Smad7 Antibody (293039) [Unconjugated]

Images

 
Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 ...read more
Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue ...read more
Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Simple Western lane view shows lysates of Sf 21 S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately56 kDa (as ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
293039
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Smad7 Antibody (293039) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Smad7
Gly320-Ser398
Accession # O15105
Specificity
Detects human, mouse, and rat Smad7.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMAD7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB2029 in the following applications:

Publications
Read Publications using
MAB2029 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad7 Antibody (293039) [Unconjugated]

  • CRCS3
  • FLJ16482
  • hSMAD7
  • MAD homolog 7
  • MAD homolog 8
  • MAD, mothers against decapentaplegic homolog 7 (Drosophila)
  • MADH7
  • MADH7mothers against decapentaplegic homolog 7
  • MADH8
  • Mothers against decapentaplegic homolog 8
  • Mothers against decapentaplegic, drosophila, homolog of, 7
  • Mothers against DPP homolog 7
  • Mothers against DPP homolog 8
  • SMAD 7
  • SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7
  • SMAD, mothers against DPP homolog 7 (Drosophila)
  • SMAD, mothers against DPP homolog 7
  • Smad7

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Smad7 Antibody (MAB2029)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ICC, IF, IHC, IHC-P, Western Blot.


Filter By Application
ICC
(1)
IF
(1)
IHC
(1)
IHC-P
(3)
Western Blot
(27)
All Applications
Filter By Species
Human
(20)
Mouse
(9)
Rat
(3)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using MAB2029 Applications Species
W Wu, XR Huang, Y You, L Xue, XJ Wang, X Meng, X Lin, J Shen, X Yu, HY Lan, H Chen Latent TGF-beta1 protects against diabetic kidney disease via Arkadia/Smad7 signaling International journal of biological sciences, 2021;17(13):3583-3594. 2021 [PMID: 34512167] (Western Blot, Mouse) Western Blot Mouse
T Wu, X Li, X Jia, Z Zhu, J Lu, H Feng, B Shen, K Guo, Y Li, Q Wang, Z Gao, B Yu, Z Ba, Y Huang, D Wu Kr�ppel like factor 10 prevents intervertebral disc degeneration via TGF-&beta signaling pathway both in vitro and in vivo Journal of Orthopaedic Translation, 2021;29(0):19-29. 2021 [PMID: 34094855] (Western Blot, Human) Western Blot Human
B Niu, J Liu, B Lv, J Lin, X Li, C Wu, X Jiang, Z Zeng, XK Zhang, H Zhou Interplay between transforming growth factor-&beta and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis Nature Communications, 2021;12(1):2809. 2021 [PMID: 33990575] (Western Blot, Human) Western Blot Human
S He, S Sun, J Lu, L Chen, X Mei, L Li, Z Zeng, M Zhong, L Xie The effects of the miR-21/SMAD7/TGF-&beta pathway on Th17 cell differentiation in COPD Scientific Reports, 2021;11(1):6338. 2021 [PMID: 33739023] (Western Blot, Human) Western Blot Human
Q Gao, Q Huang, F Li, F Luo LncRNA MCTP1-AS1 Regulates EMT Process in Endometrial Cancer by Targeting the miR-650/SMAD7 Axis OncoTargets and Therapy, 2021;14(0):751-761. 2021 [PMID: 33568915] (Western Blot, Human) Western Blot Human
F Yuan, H Yin, Y Deng, F Jiao, H Jiang, Y Niu, S Chen, H Ying, Q Zhai, Y Chen, F Guo Overexpression of Smad7 in hypothalamic POMC neurons disrupts glucose balance by attenuating central insulin signaling Mol Metab, 2020;42(0):101084. 2020 [PMID: 32971298] (Western Blot, Mouse) Western Blot Mouse
YS Han, YM Yoon, G Go, JH Lee, SH Lee Melatonin Protects Human Renal Proximal Tubule Epithelial Cells Against High Glucose-Mediated Fibrosis via the Cellular Prion Protein-TGF-beta-Smad Signaling Axis Int J Med Sci, 2020;17(9):1235-1245. 2020 [PMID: 32547319] (Western Blot, Human) Western Blot Human
DM Tufa, T Shank, AM Yingst, GD Trahan, S Shim, J Lake, R Woods, K Jones, MR Verneris Prolactin Acts on Myeloid Progenitors to Modulate SMAD7 Expression and Enhance Hematopoietic Stem Cell Differentiation into the NK Cell Lineage Sci Rep, 2020;10(1):6335. 2020 [PMID: 32286456] (Western Blot, Human) Western Blot Human
ES Di Filippo, D Costamagna, G Giacomazzi, Á Cortés-Cal, A Stryjewska, D Huylebroec, S Fulle, M Sampaolesi Zeb2 Regulates Myogenic Differentiation in Pluripotent Stem Cells Int J Mol Sci, 2020;21(7):. 2020 [PMID: 32260521] (IF, Mouse) IF Mouse
DM Barbosa, P Fahlbusch, D Herzfeld d, S Jacob, U Kettel, H Al-Hasani, M Krüger, DM Ouwens, S Hartwig, S Lehr, J Kotzka, B Knebel Rhein, a novel Histone Deacetylase (HDAC) inhibitor with antifibrotic potency in human myocardial fibrosis Sci Rep, 2020;10(1):4888. 2020 [PMID: 32184434] (Western Blot, Human) Western Blot Human
Show All 33 Publications.

Review for Smad7 Antibody (MAB2029) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB2029:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
  3
reviewed by:
Verified Customer
WB Mouse 02/16/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 22433854
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22433854

Details

Detection NotesSee PMID 22433854

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22433854

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Verified Customer
02/16/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SMAD7
Uniprot