Smad7 Antibody (293039) [Unconjugated]

Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) followed by HRP‑conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad7 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of Sf 21 S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately56 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clone: 293039
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Smad7 Antibody (293039) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Smad7
  Gly320-Ser398
  Accession # O15105
• Specificity: Detects human, mouse, and rat Smad7.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SMAD7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad7 Antibody (293039) [Unconjugated]

• CRCS3
• FLJ16482
• hSMAD7
• MAD homolog 7
• MAD homolog 8
• MAD, mothers against decapentaplegic homolog 7 (Drosophila)
• MADH7
• MADH7mothers against decapentaplegic homolog 7
• MADH8
• Mothers against decapentaplegic homolog 8
• Mothers against decapentaplegic, drosophila, homolog of, 7
• Mothers against DPP homolog 7
• Mothers against DPP homolog 8
• SMAD 7
• SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7
• SMAD, mothers against DPP homolog 7 (Drosophila)
• SMAD, mothers against DPP homolog 7
• Smad7

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Smad7 Antibody (MAB2029)(39)

Publications using MAB2029

• S Salemi, LJ Schori, T Gerwinn, M Horst, D Eberli Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction International Journal of Molecular Sciences, 2023-02-24;24(5):. 2023-02-24 [PMID: 36901894] (Western Blot, Human)
• C Maresca, G Di Maggio, C Stolfi, F Laudisi, M Colella, T Pacifico, A Di Grazia, D Di Fusco, D Congiu, AM Guida, G Sica, I Monteleone, G Monteleone Smad7 Sustains Stat3 Expression and Signaling in Colon Cancer Cells Cancers, 2022-10-12;14(20):. 2022-10-12 [PMID: 36291778] (IF/ICC, Western Blot, Human)
• SH Han, E Ballinger, SY Choung, JY Kwon Anti-Photoaging Effect of Hydrolysates from Pacific Whiting Skin via MAPK/AP-1, NF-kappaB, TGF-beta/Smad, and Nrf-2/HO-1 Signaling Pathway in UVB-Induced Human Dermal Fibroblasts Marine Drugs, 2022-04-30;20(5):. 2022-04-30 [PMID: 35621960] (Western Blot, Human)
• L Zhang, J Qu, Y Qi, Y Duan, YW Huang, Z Zhou, P Li, J Yao, B Huang, S Zhang, D Yu EZH2 engages TGFbeta signaling to promote breast cancer bone metastasis via integrin beta1-FAK activation Nature Communications, 2022-05-10;13(1):2543. 2022-05-10 [PMID: 35538070] (Western Blot, Mouse)
• W Wu, XR Huang, Y You, L Xue, XJ Wang, X Meng, X Lin, J Shen, X Yu, HY Lan, H Chen Latent TGF-beta1 protects against diabetic kidney disease via Arkadia/Smad7 signaling International journal of biological sciences, 2021-08-19;17(13):3583-3594. 2021-08-19 [PMID: 34512167] (Western Blot, Mouse)
• T Wu, X Li, X Jia, Z Zhu, J Lu, H Feng, B Shen, K Guo, Y Li, Q Wang, Z Gao, B Yu, Z Ba, Y Huang, D Wu Kr�ppel like factor 10 prevents intervertebral disc degeneration via TGF-&beta signaling pathway both in vitro and in vivo Journal of Orthopaedic Translation, 2021-05-15;29(0):19-29. 2021-05-15 [PMID: 34094855] (Western Blot, Human)
• B Niu, J Liu, B Lv, J Lin, X Li, C Wu, X Jiang, Z Zeng, XK Zhang, H Zhou Interplay between transforming growth factor-&beta and Nur77 in dual regulations of inhibitor of differentiation 1 for colonic tumorigenesis Nature Communications, 2021-05-14;12(1):2809. 2021-05-14 [PMID: 33990575] (Western Blot, Human)
• S He, S Sun, J Lu, L Chen, X Mei, L Li, Z Zeng, M Zhong, L Xie The effects of the miR-21/SMAD7/TGF-&beta pathway on Th17 cell differentiation in COPD Scientific Reports, 2021-03-18;11(1):6338. 2021-03-18 [PMID: 33739023] (Western Blot, Human, Mouse)
• Q Gao, Q Huang, F Li, F Luo LncRNA MCTP1-AS1 Regulates EMT Process in Endometrial Cancer by Targeting the miR-650/SMAD7 Axis OncoTargets and Therapy, 2021-02-03;14(0):751-761. 2021-02-03 [PMID: 33568915] (Western Blot, Human)
• F Yuan, H Yin, Y Deng, F Jiao, H Jiang, Y Niu, S Chen, H Ying, Q Zhai, Y Chen, F Guo Overexpression of Smad7 in hypothalamic POMC neurons disrupts glucose balance by attenuating central insulin signaling Mol Metab, 2020-09-22;42(0):101084. 2020-09-22 [PMID: 32971298] (Western Blot, Mouse)
• YS Han, YM Yoon, G Go, JH Lee, SH Lee Melatonin Protects Human Renal Proximal Tubule Epithelial Cells Against High Glucose-Mediated Fibrosis via the Cellular Prion Protein-TGF-beta-Smad Signaling Axis Int J Med Sci, 2020-05-18;17(9):1235-1245. 2020-05-18 [PMID: 32547319] (Western Blot, Human)
• H Ungefroren, A Kumarasing, M Musfeldt, C Fiedler, H Lehnert, JU Marquardt RAC1B Induces SMAD7 via USP26 to Suppress TGFbeta1-Dependent Cell Migration in Mesenchymal-Subtype Carcinoma Cells Cancers (Basel), 2020-06-11;12(6):. 2020-06-11 [PMID: 32545415] (Western Blot, Human)
• LD Halder, EAH Jo, MZ Hasan, M Ferreira-G, T Krüger, M Westermann, DI Palme, G Rambach, N Beyersdorf, C Speth, ID Jacobsen, O Kniemeyer, B Jungnickel, PF Zipfel, C Skerka Immune modulation by complement receptor 3-dependent human monocyte TGF-beta1-transporting vesicles Nat Commun, 2020-05-11;11(1):2331. 2020-05-11 [PMID: 32393780] (Western Blot, Human)
• DM Tufa, T Shank, AM Yingst, GD Trahan, S Shim, J Lake, R Woods, K Jones, MR Verneris Prolactin Acts on Myeloid Progenitors to Modulate SMAD7 Expression and Enhance Hematopoietic Stem Cell Differentiation into the NK Cell Lineage Sci Rep, 2020-04-14;10(1):6335. 2020-04-14 [PMID: 32286456] (Western Blot, Human)
• ES Di Filippo, D Costamagna, G Giacomazzi, Á Cortés-Cal, A Stryjewska, D Huylebroec, S Fulle, M Sampaolesi Zeb2 Regulates Myogenic Differentiation in Pluripotent Stem Cells Int J Mol Sci, 2020-04-05;21(7):. 2020-04-05 [PMID: 32260521] (IF, Mouse)
• DM Barbosa, P Fahlbusch, D Herzfeld d, S Jacob, U Kettel, H Al-Hasani, M Krüger, DM Ouwens, S Hartwig, S Lehr, J Kotzka, B Knebel Rhein, a novel Histone Deacetylase (HDAC) inhibitor with antifibrotic potency in human myocardial fibrosis Sci Rep, 2020-03-17;10(1):4888. 2020-03-17 [PMID: 32184434] (Western Blot, Human)
• W Gu, X Hong, A Le Bras, WN Nowak, S Issa Bhalo, J Deng, Y Xie, Y Hu, XZ Ruan, Q Xu Smooth muscle cells differentiated from human mesenchymal stem cells regulated by microRNA (miR)-503 and miR-222-5p are suitable for vascular tissue engineering J. Biol. Chem., 2018-04-11;0(0):. 2018-04-11 [PMID: 29643181] (Western Blot, Human)
• X Huang, Z Yue, J Wu, J Chen, S Wang, J Wu, L Ren, A Zhang, P Deng, K Wang, C Wu, X Ding, P Ye, J Xia MicroRNA-21 Knockout Exacerbates Angiotensin II-Induced Thoracic Aortic Aneurysm and Dissection in Mice With Abnormal Transforming Growth Factor-?-SMAD3 Signaling Arterioscler. Thromb. Vasc. Biol., 2018-03-08;0(0):. 2018-03-08 [PMID: 29519942] (IHC-P, Mouse)
• XL Wang, YY Zhao, L Sun, Y Shi, ZQ Li, XD Zhao, CG Xu, HG Ji, M Wang, WR Xu, W Zhu Exosomes derived from human umbilical cord mesenchymal stem cells improve myocardial repair via upregulation of Smad7 Int. J. Mol. Med., 2018-02-16;0(0):. 2018-02-16 [PMID: 29484378] (Western Blot, Rat)
• S Syed, V Dinallo, NT Iqbal, L Di Iorio, D Di Fusco, S Guleria, BC Amadi, K Sadiq, C Moskaluk, SA Ali, P Kelly, G Monteleone High SMAD7 and p-SMAD2,3 expression is associated with Environmental Enteropathy in children PLoS Negl Trop Dis, 2018-02-07;12(2):e0006224. 2018-02-07 [PMID: 29415065] (Western Blot, Human)
• V De Simone, G Bevivino, S Sedda, R Izzo, F Laudisi, V Dinallo, E Franzè, A Colantoni, A Ortenzi, S Salvatori, P Rossi, GS Sica, MC Fantini, C Stolfi, G Monteleone Smad7 knockdown activates protein kinase RNA-associated eIF2? pathway leading to colon cancer cell death Cell Death Dis, 2017-03-16;8(3):e2681. 2017-03-16 [PMID: 28300830] (ICC, Western Blot, Human)
• H Sun, Z Peng, H Tang, D Xie, Z Jia, L Zhong, S Zhao, Z Ma, Y Gao, L Zeng, R Luo, K Xie Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-? signaling in and promote progression of hepatocellular carcinoma Oncogene, 2017-02-13;0(0):. 2017-02-13 [PMID: 28192402] (IHC, Human)
• T Feng, J Dzieran, X Yuan, A Dropmann, T Maass, A Teufel, S Marhenke, T Gaiser, F Rückert, I Kleiter, S Kanzler, MP Ebert, A Vogel, P Ten Dijke, S Dooley, NM Meindl-Bei Hepatocyte-specific Smad7 deletion accelerates DEN-induced HCC via activation of STAT3 signaling in mice Oncogenesis, 2017-01-30;6(1):e294. 2017-01-30 [PMID: 28134936] (Western Blot, Human)
• CUG-binding protein 1 regulates HSC activation and liver fibrogenesis Nat Commun, 2016-11-17;7(0):13498. 2016-11-17 [PMID: 27853137] (Western Blot, Mouse)
• Boris Hinz MicroRNA-21 preserves the fibrotic mechanical memory of mesenchymal stem cells Nat Mater, 2016-10-31;0(0):. 2016-10-31 [PMID: 27798620] (Western Blot, Rat)
• The TGF?-SMAD3 pathway inhibits IL-1? induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration J Exp Clin Cancer Res, 2016-07-29;35(1):122. 2016-07-29 [PMID: 27473228] (Western Blot, Human)
• TGF-Beta Induces Endometriotic Progression via a Non-Canonical, KLF11 Mediated Mechanism Endocrinology, 2016-07-06;0(0):en20161194. 2016-07-06 [PMID: 27384304] (Western Blot, Human)
• Downregulation of the TGF-? pseudoreceptor BAMBI in non-small cell lung cancer enhances TGF-? signaling and invasion Cancer Res, 2016-05-17;0(0):. 2016-05-17 [PMID: 27197161] (IHC-P, Human)
• Bardita C, Predescu D, Sha F, Patel M, Balaji G, Predescu S Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5. J Cell Sci, 2015-02-26;128(8):1528-41. 2015-02-26 [PMID: 25720380] (Western Blot, Mouse)
• Murugaiyan G, da Cunha A, Ajay A, Joller N, Garo L, Kumaradevan S, Yosef N, Vaidya V, Weiner H MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis. J Clin Invest, 2015-02-02;125(3):1069-80. 2015-02-02 [PMID: 25642768] (Western Blot, Mouse)
• Lu G, Xu S, Peng L, Huang Z, Wang Y, Gao X Angiotensin II upregulates Kv1.5 expression through ROS-dependent transforming growth factor-beta1 and extracellular signal-regulated kinase 1/2 signalings in neonatal rat atrial myocytes. Biochem Biophys Res Commun, 2014-10-24;454(3):410-416. 2014-10-24 [PMID: 25451261] (Western Blot, Rat)
• Mehuron T, Kumar A, Duarte L, Yamauchi J, Accorsi A, Girgenrath M Dysregulation of matricellular proteins is an early signature of pathology in laminin-deficient muscular dystrophy. Skelet Muscle, 2014-07-02;4(0):14. 2014-07-02 [PMID: 25075272] (Western Blot, Mouse)
• Parikh A, Lee C, Joseph P, Marchini S, Baccarini A, Kolev V, Romualdi C, Fruscio R, Shah H, Wang F, Mullokandov G, Fishman D, D'Incalci M, Rahaman J, Kalir T, Redline R, Brown B, Narla G, DiFeo A microRNA-181a has a critical role in ovarian cancer progression through the regulation of the epithelial-mesenchymal transition. Nat Commun, 2014-01-01;5(0):2977. 2014-01-01 [PMID: 24394555] (IHC-P, Human)
• Devine D, Rostas J, Metge B, Das S, Mulekar M, Tucker J, Grizzle W, Buchsbaum D, Shevde L, Samant R Loss of N-Myc interactor promotes epithelial-mesenchymal transition by activation of TGF-beta/SMAD signaling. Oncogene, 2013-06-17;33(20):2620-8. 2013-06-17 [PMID: 23770854] (Western Blot, Human)
• Wang M, Hada M, Saha J, Sridharan D, Pluth J, Cucinotta F Protons sensitize epithelial cells to mesenchymal transition. PLoS ONE, 2012-07-23;7(7):e41249. 2012-07-23 [PMID: 22844446] (Western Blot, Human)
• Hneino M, Francois A, Buard V, Tarlet G, Abderrahmani R, Blirando K, Hoodless PA, Benderitter M, Milliat F The TGF-β/Smad repressor TG-interacting factor 1 (TGIF1) plays a role in radiation-induced intestinal injury independently of a Smad signaling pathway. PLoS ONE, 2012-05-02;7(5):e35672. 2012-05-02 [PMID: 22567107] (Western Blot, Human)
• Su RW, Lei W, Liu JL The integrative analysis of microRNA and mRNA expression in mouse uterus under delayed implantation and activation. PLoS ONE, 2010-11-29;5(11):e15513. 2010-11-29 [PMID: 21124741] (Western Blot, Mouse)
• Miyake T, Alli NS, McDermott JC Nuclear function of Smad7 promotes myogenesis. Mol. Cell. Biol., 2009-12-07;30(3):722-35. 2009-12-07 [PMID: 19995910] (ICC, Western Blot, Mouse)
• Seong HA, Jung H, Kim KT, Ha H 3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins. J. Biol. Chem., 2007-02-27;282(16):12272-89. 2007-02-27 [PMID: 17327236] (ICC, Human)

Review for Smad7 Antibody (MAB2029) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: cynthia morimoto WB Mouse 02/16/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22433854
• Species: Mouse

Bioinformatics

• Gene Symbol: SMAD7
• Uniprot:
  • O15105()