Smad7 Antibody (293039) [Unconjugated]

Images

 
Western blot shows lysates of H9 human cutaneous T lymphoma cell line, PT18 mouse mast/basophil cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Smad7 ...read more
Smad7 was detected in immersion fixed paraffin-embedded sections of human liver array using Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) at 25 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Simple Western lane view shows lysates of Sf 21 S. frugiperda insect ovarian cell line transfected with human Smad7, loaded at 0.2 mg/mL. A specific band was detected for Smad7 at approximately56 kDa (as ...read more
Smad7 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using 25 µg/mL Human/Mouse/Rat Smad7 Monoclonal Antibody (Catalog # MAB2029) overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC
Clone
293039
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Smad7 Antibody (293039) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Smad7
Gly320-Ser398
Accession # O15105
Specificity
Detects human, mouse, and rat Smad7.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SMAD7
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB2029 in the following applications:

Publications
Read Publications using
MAB2029 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad7 Antibody (293039) [Unconjugated]

  • CRCS3
  • FLJ16482
  • hSMAD7
  • MAD homolog 7
  • MAD homolog 8
  • MAD, mothers against decapentaplegic homolog 7 (Drosophila)
  • MADH7
  • MADH7mothers against decapentaplegic homolog 7
  • MADH8
  • Mothers against decapentaplegic homolog 8
  • Mothers against decapentaplegic, drosophila, homolog of, 7
  • Mothers against DPP homolog 7
  • Mothers against DPP homolog 8
  • SMAD 7
  • SMAD family member 7MAD (mothers against decapentaplegic, Drosophila) homolog 7
  • SMAD, mothers against DPP homolog 7 (Drosophila)
  • SMAD, mothers against DPP homolog 7
  • Smad7

Background

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smads 6 and 7). Smad7, also known as Mothers Against Decapentaplegic homolog 7 (MADH7), inhibits selected pathways by binding directly to cell-surface receptors and preventing the activation-induced phosphorylation of other Smad subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB2029
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC

Publications for Smad7 Antibody (MAB2029)(19)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC, IHC, IHC Paraffin-embedded, WB.


Filter By Application
ICC
(1)
IHC
(1)
IHC Paraffin-embedded
(2)
WB
(15)
All Applications
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Human
(12)
Mouse
(5)
Rat
(1)
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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using MAB2029 Applications Species
S Syed, V Dinallo, NT Iqbal, L Di Iorio, D Di Fusco, S Guleria, BC Amadi, K Sadiq, C Moskaluk, SA Ali, P Kelly, G Monteleone High SMAD7 and p-SMAD2,3 expression is associated with Environmental Enteropathy in children PLoS Negl Trop Dis, 2018;12(2):e0006224. 2018 [PMID: 29415065] (WB,, Human) WB Human
V De Simone, G Bevivino, S Sedda, R Izzo, F Laudisi, V Dinallo, E Franzè, A Colantoni, A Ortenzi, S Salvatori, P Rossi, GS Sica, MC Fantini, C Stolfi, G Monteleone Smad7 knockdown activates protein kinase RNA-associated eIF2? pathway leading to colon cancer cell death Cell Death Dis, 2017;8(3):e2681. 2017 [PMID: 28300830] (WB,, Human) WB Human
H Sun, Z Peng, H Tang, D Xie, Z Jia, L Zhong, S Zhao, Z Ma, Y Gao, L Zeng, R Luo, K Xie Loss of KLF4 and consequential downregulation of Smad7 exacerbate oncogenic TGF-? signaling in and promote progression of hepatocellular carcinoma Oncogene, 2017;0(0):. 2017 [PMID: 28192402] (IHC,, Human) IHC Human
T Feng, J Dzieran, X Yuan, A Dropmann, T Maass, A Teufel, S Marhenke, T Gaiser, F Rückert, I Kleiter, S Kanzler, MP Ebert, A Vogel, P Ten Dijke, S Dooley, NM Meindl-Bei Hepatocyte-specific Smad7 deletion accelerates DEN-induced HCC via activation of STAT3 signaling in mice Oncogenesis, 2017;6(1):e294. 2017 [PMID: 28134936] (WB,, Human) WB Human
CUG-binding protein 1 regulates HSC activation and liver fibrogenesis Nat Commun, 2016;7(0):13498. 2016 [PMID: 27853137] (WB, ) WB
The TGF?-SMAD3 pathway inhibits IL-1? induced interactions between human pancreatic stellate cells and pancreatic carcinoma cells and restricts cancer cell migration J Exp Clin Cancer Res, 2016;35(1):122. 2016 [PMID: 27473228] (WB,, Human) WB Human
TGF-Beta Induces Endometriotic Progression via a Non-Canonical, KLF11 Mediated Mechanism Endocrinology, 2016;0(0):en20161194. 2016 [PMID: 27384304] (WB,, Human) WB Human
Downregulation of the TGF-? pseudoreceptor BAMBI in non-small cell lung cancer enhances TGF-? signaling and invasion Cancer Res, 2016;0(0):. 2016 [PMID: 27197161] (IHC Paraffin-embedded,, Human) IHC Paraffin-embedded Human
Bardita C, Predescu D, Sha F, Patel M, Balaji G, Predescu S Endocytic deficiency induced by ITSN-1s knockdown alters the Smad2/3-Erk1/2 signaling balance downstream of Alk5. J Cell Sci, 2015;128(8):1528-41. 2015 [PMID: 25720380] (WB,, Mouse) WB Mouse
Murugaiyan G, da Cunha A, Ajay A, Joller N, Garo L, Kumaradevan S, Yosef N, Vaidya V, Weiner H MicroRNA-21 promotes Th17 differentiation and mediates experimental autoimmune encephalomyelitis. J Clin Invest, 2015;125(3):1069-80. 2015 [PMID: 25642768] (WB,, Mouse) WB Mouse
Show All 19 Publications.

Review for Smad7 Antibody (MAB2029) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB2029:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 02/16/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22433854
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22433854

Details

Detection NotesSee PMID 22433854

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22433854

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Recent Reviews

3
5
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4
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1
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1
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Anonymous
02/16/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SMAD7
Entrez
Uniprot