Recombinant Human Noggin Protein

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Recombinant human Noggin (Catalog # 6057-NG) has a molecular weight (MW) of 58.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational ...read more
BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3 day incubation ...read more
Recombinant Human Noggin (Catalog # 6057-NG) inhibits BMP-4 induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The activity is approximately 30-fold greater than the top competitor's ...read more
The lot-to-lot consistency of Recombinant Human Noggin (Catalog # 6057-NG) was assessed by testing the ability of three independent lots of the protein to inhibit BMP-4-induced alkaline phosphatase production in the ...read more
iPSC-derived cerebral organoids (day 45) were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and brain organoid culture medium, which includes Recombinant Human FGF-basic (3718-FB) and ...read more
iPSC-derived cerebral organoids (day 45) were cultured using Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and brain organoid culture medium, which includes Recombinant Human FGF-basic (3718-FG) and ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Noggin Protein Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.0200-0.160 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived human Noggin protein
Gln28-Cys232
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NOG
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
23 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-33 kDa, reducing conditions
Publications
Read Publications using
6057-NG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS  containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Noggin Protein

  • NOG
  • Noggin
  • SYM1
  • symphalangism 1 (proximal)
  • synostoses (multiple) syndrome 1
  • SYNS1
  • SYNS1A

Background

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons (6, 9 - 13). Noggin also appears to maintain adult stem cell populations in-vivo, for example, maintaining neural stem cells within the hippocampus (13).

  1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  2. Groppe, J. et al. (2002) Nature 420:636.
  3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
  4. Brunet, L. J. et al. (1998) Science 280:1455.
  5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
  6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
  7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
  8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
  9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
  10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
  11. Chaturvedi, G. et al. (2009) Cell Prolif. 42:425.
  12. Chiba, S. et al. (2008) Stem Cells 26:2810.
  13. Bonaguidi, M.A. et al. (2008) J. Neurosci. 28:9194.

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Publications for Noggin (6057-NG)(192)

We have publications tested in 17 confirmed species: Human, Mouse, Rat, Chicken, Equine, Feline, Frog, N/A, Porcine, Primate, Primate - Macaca mulatta (Rhesus Macaque), Primate - Pan troglodytes (Chimpanzee), Primate - Pongo (Orangutan), Xenograft, Xenopus, Zebrafish,  Human.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Tissue Culture.


Filter By Application
Bioassay
(178)
Cell Culture
(7)
Differentiation
(4)
In Vivo
(4)
Tissue Culture
(1)
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Human
(162)
Mouse
(13)
Rat
(2)
Chicken
(4)
Equine
(2)
Feline
(1)
Frog
(1)
N/A
(1)
Porcine
(1)
Primate
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Pan troglodytes (Chimpanzee)
(1)
Primate - Pongo (Orangutan)
(1)
Xenograft
(1)
Xenopus
(1)
Zebrafish
(1)
 Human
(1)
All Species
Showing Publications 1 - 10 of 192. Show All 192 Publications.
Publications using 6057-NG Applications Species
Hsu, WH;LaBella, KA;Lin, Y;Xu, P;Lee, R;Hsieh, CE;Yang, L;Zhou, A;Blecher, JM;Wu, CJ;Lin, K;Shang, X;Jiang, S;Spring, DJ;Xia, Y;Chen, P;Shen, JP;Kopetz, S;DePinho, RA; Oncogenic KRAS drives lipo-fibrogenesis to promote angiogenesis and colon cancer progression Cancer discovery 2023-09-28 [PMID: 37768068] (Bioassay, Xenograft) Bioassay Xenograft
Lassé, M;El Saghir, J;Berthier, CC;Eddy, S;Fischer, M;Laufer, SD;Kylies, D;Hutzfeldt, A;Bonin, LL;Dumoulin, B;Menon, R;Vega-Warner, V;Eichinger, F;Alakwaa, F;Fermin, D;Billing, AM;Minakawa, A;McCown, PJ;Rose, MP;Godfrey, B;Meister, E;Wiech, T;Noriega, M;Chrysopoulou, M;Brandts, P;Ju, W;Reinhard, L;Hoxha, E;Grahammer, F;Lindenmeyer, MT;Huber, TB;Schlüter, H;Thiel, S;Mariani, LH;Puelles, VG;Braun, F;Kretzler, M;Demir, F;Harder, JL;Rinschen, MM; An integrated organoid omics map extends modeling potential of kidney disease Nature communications 2023-08-14 [PMID: 37580326] (Bioassay, Human) Bioassay Human
Mubariz, F;Saadin, A;Lingenfelter, N;Sarkar, C;Banerjee, A;Lipinski, MM;Awad, O; Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease Frontiers in neuroscience 2023-06-02 [PMID: 37332877] (Bioassay, Human) Bioassay Human
Kuo, C;Choi, S;van Unen, V;Zhang, H;Rustagi, A;Alwahabi, S;Santos, A;Chan, J;Lam, B;Solis, D;Mah, J;R�ltgen, K;Trope, W;Guh-Siesel, A;Lin, Z;Beck, A;Edwards, C;Mallajosyula, V;Martin, B;Dunn, J;Shrager, J;Baric, R;Pinsky, B;Boyd, S;Blish, C;Davis, M; Organoid modeling of lung-resident immune responses to SARS-CoV-2 infection Research square 2023-05-05 [PMID: 37205380] (Bioassay, Human) Bioassay Human
Yamakawa, K;Koyanagi-Aoi, M;Machinaga, A;Kakiuchi, N;Hirano, T;Kodama, Y;Aoi, T; Blockage of retinoic acid signaling via RAR? suppressed the proliferation of pancreatic cancer cells by arresting the cell cycle progression of the G1-S phase Cancer cell international 2023-05-17 [PMID: 37198667] (Cell Culture, Human) Cell Culture  Human
Darwish, NHE;Hussein, KA;Elmasry, K;Ibrahim, AS;Humble, J;Moustafa, M;Awadalla, F;Al-Shabrawey, M; Bone Morphogenetic Protein-4 Impairs Retinal Endothelial Cell Barrier, a Potential Role in Diabetic Retinopathy Cells 2023-04-28 [PMID: 37174679] (Bioassay, Human) Bioassay Human
Matkovic Leko, I;Schneider, RT;Thimraj, TA;Schrode, N;Beitler, D;Liu, HY;Beaumont, K;Chen, YW;Snoeck, HW; A distal lung organoid model to study interstitial lung disease, viral infection and human lung development Nature protocols 2023-05-10 [PMID: 37165073] (Bioassay, Human) Bioassay Human
X Huang, T Nero, R Weeraseker, KH Matej, A Hinbest, Z Jiang, RF Lee, L Wu, C Chak, J Nijjer, I Gibaldi, H Yang, N Gamble, WL Ng, SA Malaker, K Sumigray, R Olson, J Yan Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization Nature Communications, 2023-04-13;14(1):2104. 2023-04-13 [PMID: 37055389] (Bioassay, Mouse) Bioassay Mouse
AH Ghobashi, TT Vuong, JW Kimani, HM O'Hagan Activation of AKT induces EZH2-mediated beta-catenin trimethylation in colorectal cancer bioRxiv : the preprint server for biology, 2023-02-04;0(0):. 2023-02-04 [PMID: 36778289] (Bioassay, Human) Bioassay Human
J Tian, JH Chen, SX Chao, K Pelka, M Giannakis, J Hess, K Burke, V Jorgji, P Sindurakar, J Braverman, A Mehta, T Oka, M Huang, D Lieb, M Spurrell, JN Allen, TA Abrams, JW Clark, AC Enzinger, PC Enzinger, SJ Klempner, NJ McCleary, JA Meyerhardt, DP Ryan, MB Yurgelun, K Kanter, EE Van Sevent, I Baiev, G Chi, J Jarnagin, WB Bradford, E Wong, AG Michel, IJ Fetter, G Siravegna, AJ Gemma, A Sharpe, S Demehri, R Leary, CD Campbell, O Yilmaz, GA Getz, AR Parikh, N Hacohen, RB Corcoran Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trial Nature Medicine, 2023-01-26;0(0):. 2023-01-26 [PMID: 36702949] (Bioassay, Human) Bioassay Human
Show All 192 Publications.

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Bioinformatics

Gene Symbol NOG
Uniprot