Recombinant Human Noggin Protein


BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3 day incubation more

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Recombinant Human Noggin Protein Summary

Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.04‑0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).
Mouse myeloma cell line, NS0-derived human Noggin protein
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
23 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
30-33 kDa, reducing conditions
Read Publications using
6057-NG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS  containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Noggin Protein

  • NOG
  • Noggin
  • SYM1
  • symphalangism 1 (proximal)
  • synostoses (multiple) syndrome 1
  • SYNS1


Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons (6, 9 - 13). Noggin also appears to maintain adult stem cell populations in-vivo, for example, maintaining neural stem cells within the hippocampus (13).

  1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  2. Groppe, J. et al. (2002) Nature 420:636.
  3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
  4. Brunet, L. J. et al. (1998) Science 280:1455.
  5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
  6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
  7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
  8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
  9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
  10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
  11. Chaturvedi, G. et al. (2009) Cell Prolif. 42:425.
  12. Chiba, S. et al. (2008) Stem Cells 26:2810.
  13. Bonaguidi, M.A. et al. (2008) J. Neurosci. 28:9194.

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Publications for Noggin (6057-NG)(82)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Primate, Xenopus, Zebrafish.

We have publications tested in 2 applications: Bioassay, In Vivo.

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Showing Publications 1 - 10 of 82. Show All 82 Publications.
Publications using 6057-NG Applications Species
Y Zhang, Y Yang, M Jiang, SX Huang, W Zhang, D Al Alam, S Danopoulos, M Mori, YW Chen, R Balasubram, SM Chuva de S, C Serra, M Bialecka, E Kim, S Lin, ALR Toste de C, PN Riccio, WV Cardoso, X Zhang, HW Snoeck, J Que 3D Modeling of Esophageal Development using Human PSC-Derived Basal Progenitors Reveals a Critical Role for Notch Signaling Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30244870] (Bioassay, Human) Bioassay Human
R Cruz-Acuña, M Quirós, S Huang, D Siuda, JR Spence, A Nusrat, AJ García PEG-4MAL hydrogels for human organoid generation, culture, and in vivo delivery Nat Protoc, 2018;0(0):. 2018 [PMID: 30190557] (Bioassay, Human) Bioassay Human
MA Scavuzzo, MC Hill, J Chmielowie, D Yang, J Teaw, K Sheng, Y Kong, M Bettini, C Zong, JF Martin, M Borowiak Endocrine lineage biases arise in temporally distinct endocrine progenitors during pancreatic morphogenesis Nat Commun, 2018;9(1):3356. 2018 [PMID: 30135482] (Bioassay, Human) Bioassay Human
JD Blair, D Hockemeyer, HS Bateup Genetically engineered human cortical spheroid models of tuberous sclerosis Nat. Med., 2018;0(0):. 2018 [PMID: 30127391] (Bioassay, Human) Bioassay Human
S Haider, G Meinhardt, L Saleh, V Kunihs, M Gamperl, U Kaindl, A Ellinger, TR Burkard, C Fiala, J Pollheimer, S Mendjan, PA Latos, M Knöfler Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta Stem Cell Reports, 2018;0(0):. 2018 [PMID: 30078556] (Bioassay, Human) Bioassay Human
Y Liu, A Antonic, X Yang, N Korte, K Lim, AE Michalska, M Dottori, DW Howells Derivation of phenotypically diverse neural culture from hESC by combining adherent and dissociation methods J. Neurosci. Methods, 2018;0(0):. 2018 [PMID: 30003885] (Bioassay, Human) Bioassay Human
S Chen, L Jia, S Zhang, Y Zheng, Y Zhou DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis Stem Cell Res Ther, 2018;9(1):185. 2018 [PMID: 29973283] (In Vivo, Mouse) In Vivo Mouse
KKB Tan, WWM Lim, C Chai, M Kukumberg, KL Lim, ELK Goh, EKF Yim Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells Sci Rep, 2018;8(1):9567. 2018 [PMID: 29934644] (Bioassay, Human) Bioassay Human
MJ Workman, JP Gleeson, EJ Troisi, HQ Estrada, SJ Kerns, CD Hinojosa, GA Hamilton, SR Targan, CN Svendsen, RJ Barrett Enhanced Utilization of Induced Pluripotent Stem Cell-Derived Human Intestinal Organoids Using Microengineered Chips Cell Mol Gastroenterol Hepatol, 2018;5(4):669-677.e2. 2018 [PMID: 29930984] (Bioassay, Human) Bioassay Human
F Zambelli, J Mertens, D Dziedzicka, J Sterckx, C Markouli, A Keller, P Tropel, L Jung, S Viville, H Van de Vel, M Geens, S Seneca, K Sermon, C Spits Random Mutagenesis, Clonal Events, and Embryonic or Somatic Origin Determine the mtDNA Variant Type and Load in Human Pluripotent Stem�Cells Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29910126] (Bioassay, Human) Bioassay Human
Show All 82 Publications.

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Gene Symbol NOG