Recombinant Human Noggin Protein

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Recombinant Human Noggin (Catalog # 6057-NG) inhibits BMP-4 induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The activity is approximately 30-fold greater than the top competitor's ...read more
BG01V human embryonic stem cells were cultured in Mouse Embryonic Fibroblast Conditioned Media supplemented with FGF basic (5 ng/mL). Stem cells were driven into early cells of the neuroectoderm using a 3 day incubation ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Noggin Protein Summary

Details of Functionality
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.02-0.16 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP‑4 (Catalog # 314-BP).
Source
Mouse myeloma cell line, NS0-derived human Noggin protein
Gln28-Cys232
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NOG
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
23 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
30-33 kDa, reducing conditions
Publications
Read Publications using
6057-NG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in PBS  containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Noggin Protein

  • NOG
  • Noggin
  • SYM1
  • symphalangism 1 (proximal)
  • synostoses (multiple) syndrome 1
  • SYNS1
  • SYNS1A

Background

Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs) (1, 2). Human Noggin cDNA encodes a 232 amino acid (aa) precursor protein; cleavage of a 19 aa signal peptide generates the 213 aa mature protein which contains an N-terminal acidic region, a central basic heparin‑binding segment and a C-terminal cysteine-knot structure (2). Secreted Noggin probably remains close to the cell surface due to its binding of heparin‑containing proteoglycans (3). Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99%, 99%, 98%, 97% and 89% aa sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively. Noggin binds some BMPs such as BMP-4 with high affinity and others such as BMP-7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors (2, 4). During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning (5-7). During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints (4). Mutations within the cysteine-knot region of human Noggin are linked to multiple types of skeletal dysplasias that result in apical joint fusions (8). Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin (1). During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons (6, 9 - 13). Noggin also appears to maintain adult stem cell populations in-vivo, for example, maintaining neural stem cells within the hippocampus (13).

  1. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  2. Groppe, J. et al. (2002) Nature 420:636.
  3. Paine-Saunders, S et al. (2002) J. Biol. Chem. 277:2089.
  4. Brunet, L. J. et al. (1998) Science 280:1455.
  5. McMahon, J. A. et al. (1998) Genes Dev. 12:1438.
  6. Itsykson, P. et al. (2005) Mol. Cell. Neurosci. 30:24.
  7. Yuasa, S. et al. (2005) Nat. Biotechnol. 23:607.
  8. Gong, Y. et al. (1999) Nat. Genet. 21:302.
  9. Xu, R.-H. et al. (2005) Nat. Methods 2:185.
  10. Wang, G. et al. (2005) Biochem. Biophys. Res. Commun. 330:934.
  11. Chaturvedi, G. et al. (2009) Cell Prolif. 42:425.
  12. Chiba, S. et al. (2008) Stem Cells 26:2810.
  13. Bonaguidi, M.A. et al. (2008) J. Neurosci. 28:9194.

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6057-NG
Species: Hu
Applications: Bioactivity

Publications for Noggin (6057-NG)(101)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Chicken, Primate, Xenopus, Zebrafish.

We have publications tested in 3 applications: Bioassay, Differentiation, In Vivo.


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Bioassay
(94)
Differentiation
(2)
In Vivo
(3)
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Human
(87)
Mouse
(6)
Rat
(1)
Chicken
(3)
Primate
(1)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 101. Show All 101 Publications.
Publications using 6057-NG Applications Species
KB Jung, O Kwon, MO Lee, H Lee, YS Son, O Habib, JH Oh, HS Cho, CR Jung, J Kim, MY Son Blockade of STAT3 Causes Severe In Vitro and In Vivo Maturation Defects in Intestinal Organoids Derived from Human Embryonic Stem Cells J Clin Med, 2019;8(7):. 2019 [PMID: 31277507] (Bioassay, Human) Bioassay Human
AM Blázquez-M, M Jumabay, P Rajbhandar, T Sallam, Y Guo, J Yao, L Vergnes, K Reue, L Zhang, Y Yao, AM Fogelman, P Tontonoz, AJ Lusis, X Wu, KI Boström Noggin depletion in adipocytes promotes obesity in mice Mol Metab, 2019;0(0):. 2019 [PMID: 31027994] (Bioassay, Mouse) Bioassay Mouse
X Wang, M Sterr, Ansarullah, I Burtscher, A Böttcher, J Beckenbaue, J Siehler, T Meitinger, HU Häring, H Staiger, FM Cernilogar, G Schotta, M Irmler, J Beckers, CVE Wright, M Bakhti, H Lickert Point mutations in the PDX1 transactivation domain impair human ?-cell development and function Mol Metab, 2019;0(0):. 2019 [PMID: 30930126] (Differentiation, Human) Differentiation Human
M Iwashita, H Ohta, T Fujisawa, M Cho, M Ikeya, S Kidoaki, Y Kosodo Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells Sci Rep, 2019;9(1):3068. 2019 [PMID: 30816128] (Bioassay, Human) Bioassay Human
V Chesnokova, S Zonis, R Barrett, H Kameda, K Wawrowsky, A Ben-Shlomo, M Yamamoto, J Gleeson, C Bresee, V Gorbunova, S Melmed Excess growth hormone suppresses DNA damage repair in epithelial cells JCI Insight, 2019;4(3):. 2019 [PMID: 30728323] (Bioassay, Human) Bioassay Human
AJ Miller, BR Dye, D Ferrer-Tor, DR Hill, AW Overeem, LD Shea, JR Spence Generation of lung organoids from human pluripotent stem cells in vitro Nat Protoc, 2019;14(2):518-540. 2019 [PMID: 30664680] (Bioassay, Human) Bioassay Human
J Ruzicka, N Romanyuk, K Jirakova, A Hejcl, O Janouskova, LU Machova, M Bochin, M Pradny, L Vargova, P Jendelova The Effect of iPS-Derived Neural Progenitors Seeded on Laminin-Coated pHEMA-MOETACl Hydrogel with Dual Porosity in a Rat Model of Chronic Spinal Cord Injury Cell Transplant, 2019;0(0):9636897188237. 2019 [PMID: 30654639] (Bioassay, Human) Bioassay Human
AR Field, FMJ Jacobs, IT Fiddes, APR Phillips, AM Reyes-Orti, E LaMontagne, L Whitehead, V Meng, JL Rosenkrant, M Olsen, M Hauessler, S Katzman, SR Salama, D Haussler Structurally Conserved Primate LncRNAs Are Transiently Expressed during Human Cortical Differentiation and Influence Cell-Type-Specific Genes Stem Cell Reports, 2019;12(2):245-257. 2019 [PMID: 30639214] (Bioassay, Human) Bioassay Human
STJ Bradford, EJ Ranghini, E Grimley, PH Lee, GR Dressler High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30602563] (Bioassay, Human) Bioassay Human
MA Scavuzzo, J Chmielowie, D Yang, K Wamble, LS Chaboub, L Duraine, B Tepe, SM Glasgow, BR Arenkiel, C Brou, B Deneen, M Borowiak Pancreatic Cell Fate Determination Relies on Notch Ligand Trafficking by NFIA Cell Rep, 2018;25(13):3811-3827.e7. 2018 [PMID: 30590051] (Bioassay, Human) Bioassay Human
Show All 101 Publications.

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Bioinformatics

Gene Symbol NOG
Uniprot