Recombinant Human BMP-7 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-7 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.1-0.6 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-7 protein
Ser293-His431
Accession #
N-terminal Sequence
Ser293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP7
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-20 kDa, reducing conditions
Publications
Read Publications using
354-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-7 Protein

  • BMP7
  • BMP-7
  • bone morphogenetic protein 7
  • Eptotermin alfa
  • OP-1
  • OP-1OP1
  • Osteogenic protein 1

Background

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (5, 6). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (7-9). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (6). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (5). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13-15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).
  1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
  2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
  3. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  4. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  6. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
  7. Israel, D.I. et al. (1996) Growth Factors 13:291.
  8. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  9. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  10. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
  11. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
  12. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
  13. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
  14. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
  15. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
  16. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

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Applications: Bioactivity

Publications for BMP-7 (354-BP)(70)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Porcine, Xenopus, Zebrafish.

We have publications tested in 7 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ELISA Capture, In Vivo, Surface Plasmon Resonance.


Filter By Application
Binding Assay
(2)
Bioassay
(56)
Cell Culture
(1)
ELISA (Capture)
(1)
ELISA Capture
(1)
In Vivo
(6)
Surface Plasmon Resonance
(2)
All Applications
Filter By Species
Human
(37)
Mouse
(18)
Rat
(4)
Bovine
(2)
Chicken
(3)
Porcine
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 70. Show All 70 Publications.
Publications using 354-BP Applications Species
M Ali, F Kabir, JJ Thomson, Y Ma, C Qiu, M Delannoy, SY Khan, SA Riazuddin Comparative transcriptome analysis of hESC- and iPSC-derived lentoid bodies Sci Rep, 2019;9(1):18552. 2019 [PMID: 31811247] (Bioassay, Human) Bioassay Human
M Omi, V Kaartinen, Y Mishina Activin A receptor type 1-mediated BMP signaling regulates RANKL-induced osteoclastogenesis via canonical SMAD signaling pathway J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31619522] (Bioassay, Mouse) Bioassay Mouse
T Kamperman, M Koerselman, C Kelder, J Hendriks, JF Crispim, X de Peuter, PJ Dijkstra, M Karperien, J Leijten Spatiotemporal material functionalization via competitive supramolecular complexation of avidin and biotin analogs Nat Commun, 2019;10(1):4347. 2019 [PMID: 31554812] (ELISA Capture, Human) ELISA Capture Human
S Muller, I Ader, J Creff, H Leménager, P Achard, L Casteilla, L Sensebé, A Carrière, F Deschaseau Human adipose stromal-vascular fraction self-organizes to form vascularized adipose tissue in 3D cultures Sci Rep, 2019;9(1):7250. 2019 [PMID: 31076601] (Human) Human
Á Klusóczki, Z Veréb, A Vámos, P Fischer-Po, M Wabitsch, Z Bacso, L Fésüs, E Kristóf Differentiating SGBS adipocytes respond to PPAR? stimulation, irisin and BMP7 by functional browning and beige characteristics Sci Rep, 2019;9(1):5823. 2019 [PMID: 30967578] (Bioassay, Human) Bioassay Human
MJ Harms, Q Li, S Lee, C Zhang, B Kull, S Hallen, A Thorell, I Alexanders, CE Hagberg, XR Peng, A Mardinoglu, KL Spalding, J Boucher Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes Cell Rep, 2019;27(1):213-225.e5. 2019 [PMID: 30943403] (Bioassay, Human) Bioassay Human
WKW Ho, L Freem, D Zhao, KJ Painter, TE Woolley, EA Gaffney, MJ McGrew, A Tzika, MC Milinkovit, P Schneider, A Drusko, F Matthäus, JD Glover, KL Wells, JA Johansson, MG Davey, HM Sang, M Clinton, DJ Headon Feather arrays are patterned by interacting signalling and cell density waves PLoS Biol., 2019;17(2):e3000132. 2019 [PMID: 30789897] (Bioassay, Chicken) Bioassay Chicken
M Kijewska, C Viski, F Turrell, A Fitzpatric, A van Weverw, Q Gao, M Iravani, CM Isacke Using an in-vivo syngeneic spontaneous metastasis model identifies ID2 as a promoter of breast cancer colonisation in the brain Breast Cancer Res., 2019;21(1):4. 2019 [PMID: 30642388] (Bioassay, Mouse) Bioassay Mouse
CC Weng, PY Ding, YH Liu, JR Hawse, M Subramania, CC Wu, YC Lin, CY Chen, WC Hung, KH Cheng Mutant Kras-induced upregulation of CD24 enhances prostate cancer stemness and bone metastasis Oncogene, 2018;0(0):. 2018 [PMID: 30467381] (Bioassay, Mouse) Bioassay Mouse
X Huang, L Zhong, JN Post, M Karperien Co-treatment of TGF-?3 and BMP7 is superior in stimulating chondrocyte redifferentiation in both hypoxia and normoxia compared to single treatments Sci Rep, 2018;8(1):10251. 2018 [PMID: 29980690] (Bioassay, Human) Bioassay Human
Show All 70 Publications.

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Bioinformatics

Gene Symbol BMP7
Uniprot
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