Recombinant Human BMP-7 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human BMP-7 Protein Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50 for this effect is 0.1-0.6 µg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human BMP-7 protein
Ser293-His431
Accession #
N-terminal Sequence
Ser293
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
BMP7
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-20 kDa, reducing conditions
Publications
Read Publications using
354-BP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BMP-7 Protein

  • BMP7
  • BMP-7
  • bone morphogenetic protein 7
  • Eptotermin alfa
  • OP-1
  • OP-1OP1
  • Osteogenic protein 1

Background

Bone morphogenetic protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), is a widely expressed TGF-beta superfamily member with important functions during embryogenesis, in the adult, and in disease (1, 2). Human BMP-7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain (3, 4). The growth factor domain of human BMP-7 shares 98% aa sequence identity with mouse and rat BMP-7. The BMP-7 propeptide is cleaved intracellularly but remains in association with the growth factor domain. BMP-7 is subsequently secreted as a tetramer that consists of two propeptides and two disulfide-linked growth factor domains (5, 6). Mature BMP-7 can also form disulfide-linked heterodimers with BMP-2 or BMP-4, complexes that show increased potency and range of activity compared to BMP-7 homodimers (7-9). The presence of the propeptides in the BMP-7 tetramer does not diminish the bioactivity of the growth factor domains (6). Secreted BMP-7 is immobilized in the extracellular matrix as a result of interactions between the propeptide and matrix Fibrillin (5). BMP-7 exerts its biological effects through the type 2 receptors Activin RIIA, Activin RIIB, and BMPR-II and the type 1 receptors Activin RIA, BMPR-IA, and BMPR-IB (2, 6). BMP-7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development (10, 11), inhibits the branching of prostate epithelium (12), and antagonizes epithelial-mesenchymal transition (EMT) (13-15). In pathological conditions, BMP-7 inhibits tumor growth and metastasis (14), ameliorates fibrotic damage in nephritis (13), and promotes neuroregeneration following brain ischemia (16).
  1. Carreira, A.C. et al. (2015) J. Dent. Res. 93:335.
  2. Weiskirchen, R. and S.K. Meurer (2013) Front. Biosci. (Landmark Ed.) 18:1407.
  3. Ozkaynak, E. et al. (1990) EMBO J. 9:2085.
  4. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. USA 87:9843.
  5. Gregory, K.E. et al. (2005) J. Biol. Chem. 280:27970.
  6. Sengle, G. et al. (2008) J. Mol. Biol. 381:1025.
  7. Israel, D.I. et al. (1996) Growth Factors 13:291.
  8. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  9. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  10. Sampath, T.K. et al. (1992) J. Biol. Chem. 267:20352.
  11. Kazama, I. et al. (2008) J. Am. Soc. Nephrol. 19:2181.
  12. Grishina, I.B. et al. (2005) Dev. Biol. 288:334.
  13. Zeisberg, M. et al. (2003) Nat. Med. 9:964.
  14. Buijs, J.T. et al. (2007) Am. J. Pathol. 171:1047.
  15. Yu, M.-A. et al. (2009) J. Am. Soc. Nephrol. 20:567.
  16. Chou, J. et al. (2006) J. Neurol. Sci. 240:21.

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354-BP
Species: Hu
Applications: Bioactivity

Publications for BMP-7 (354-BP)(59)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Porcine, Xenopus, Zebrafish.

We have publications tested in 7 applications: Binding Assay, Bioassay, ELISA capture, In Vivo, Surface Plasmon Resonance, cell culture, complex formation.


Filter By Application
Binding Assay
(1)
Bioassay
(47)
ELISA capture
(1)
In Vivo
(6)
Surface Plasmon Resonance
(2)
cell culture
(1)
complex formation
(1)
All Applications
Filter By Species
Human
(31)
Mouse
(15)
Rat
(4)
Bovine
(2)
Chicken
(2)
Porcine
(1)
Xenopus
(3)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 59. Show All 59 Publications.
Publications using 354-BP Applications Species
C Chen, GC Bai, HL Jin, K Lei, KX Li Local injection of bone morphogenetic protein 7 promotes neuronal regeneration and motor function recovery after acute spinal cord injury Neural Regen Res, 2018;13(6):1054-1060. 2018 [PMID: 29926833] (In Vivo, Rat) In Vivo Rat
K Hino, K Horigome, M Nishio, S Komura, S Nagata, C Zhao, Y Jin, K Kawakami, Y Yamada, A Ohta, J Toguchida, M Ikeya Activin-A enhances mTOR signaling to promote aberrant chondrogenesis in fibrodysplasia ossificans progressiva J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28758906] (In Vivo, Mouse) In Vivo Mouse
C Sallon, I Callebaut, I Boulay, J Fontaine, D Logeart-Av, C Henriquet, M Pugnière, X Cayla, P Monget, G Harichaux, V Labas, S Canepa, C Taragnat Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells J. Biol. Chem., 2017;0(0):. 2017 [PMID: 28747434] (Bioassay, Mouse) Bioassay Mouse
P Wongwitwic, J Kaewsricha Osteogenic differentiation of mesenchymal stem cells is impaired by bone morphogenetic protein 7 Adv Med Sci, 2017;62(2):266-272. 2017 [PMID: 28501726] (Bioassay, Rat) Bioassay Rat
LK Bollum, K Huse, MP Oksvold, B Bai, VI Hilden, L Forfang, SO Yoon, S Wälchli, EB Smeland, JH Myklebust BMP-7 induces apoptosis in human germinal center B cells and is influenced by TGF-? receptor type I ALK5 PLoS ONE, 2017;12(5):e0177188. 2017 [PMID: 28489883] (Bioassay, Human) Bioassay Human
E Raja, A Komuro, R Tanabe, S Sakai, Y Ino, N Saito, T Todo, M Morikawa, H Aburatani, D Koinuma, C Iwata, K Miyazono Bone morphogenetic protein signaling mediated by ALK-2 and DLX2 regulates apoptosis in glioma-initiating cells Oncogene, 2017;0(0):. 2017 [PMID: 28459464] (Bioassay, Human) Bioassay Human
DW Hammers, M Merscham-B, JY Hsiao, S Engst, JJ Hartman, HL Sweeney Supraphysiological levels of GDF11 induce striated muscle atrophy EMBO Mol Med, 2017;0(0):. 2017 [PMID: 28270449] (Bioassay, Human) Bioassay Human
A Shiraishi, K Muguruma, Y Sasai Generation of thalamic neurons from mouse embryonic stem cells Development, 2017;0(0):. 2017 [PMID: 28219951] (Bioassay, Mouse) Bioassay Mouse
T Chattopadh, RR Singh, S Gupta, A Surolia Bone morphogenetic protein-7 (BMP-7) augments insulin sensitivity in mice with type II diabetes mellitus by potentiating PI3K/AKT pathway Biofactors, 2017;0(0):. 2017 [PMID: 28186649] (In Vivo, Mouse) In Vivo Mouse
Tumor-Derived Factors and Reduced p53 Promote Endothelial Cell Centrosome Over-Duplication PLoS ONE, 2016;11(12):e0168334. 2016 [PMID: 27977771] (Bioassay, Human) Bioassay Human
Show All 59 Publications.

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Bioinformatics

Gene Symbol BMP7
Entrez
Uniprot