Smad9 Antibody

Images

 
Smad9 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Smad9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7215) at 3 µg/mL overnight at 4 ...read more
Smad9 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human Smad9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7215) at 1.7 µg/mL overnight at 4 °C. Tissue ...read more
Smad9 was detected in immersion fixed A172 human glioblastoma cell line using Sheep Anti-Human/Mouse Smad9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7215) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications IHC, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Smad9 Antibody Summary

Immunogen
E. coli-derived recombinant human Smad9
Ala158-Ser223
Accession # O15198
Specificity
Detects human Smad9 in direct ELISAs. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Smad1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SMAD9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Smad9 Antibody

  • MAD homolog 9
  • MAD, mothers against decapentaplegic homolog 9 (Drosophila)
  • Madh6
  • MADH6mothers against decapentaplegic homolog 9
  • MADH9
  • MADH9Mothers against decapentaplegic, drosophila, homolog of, 9
  • Mothers against DPP homolog 9
  • SMAD 9
  • SMAD family member 9SMAD8B
  • SMAD, mothers against DPP homolog 9 (Drosophila)
  • SMAD, mothers against DPP homolog 9
  • SMAD8
  • SMAD8A
  • Smad9

Background

SMAD9 (SMA protein and Mothers Against Decapentaplegic 9; also SMAD8 and MAHD6) is a 55-60 kDa R-Group member of the dwarfin/SMAD family of proteins. It is expressed by BMP-responsive cells, and serves to transmit information from the BMP receptor (BMPR) to the nucleus. In particular, following BMPR activation, SMAD9 is phosphorylated by the BMPR on its C-terminus, promoting heterodimerization with SMAD4 and its translocation into the nucleus. Here, SMAD9 binds to DNA and participates in gene activation. Human SMAD9 is 467 amino acids (aa) in length. It contains a DNA-binding domain termed MH1 (aa 16-140) plus a receptor/transcription factor binding domain termed MH2 (aa 273-467). The C-terminus contains two phosphorylation sites at Ser465 and Ser467. There is one splice form that shows a deletion of aa 224-260. Over aa 158-223, human and mouse SMAD9 share 82% identy in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SMAD9
Uniprot