Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF ...read more
ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ID1 at approximately 30 kDa (as indicated) using 20 µg/mL of Goat ...read more
ID1 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative) using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for ID1 Antibody [Unconjugated]
bHLHb24DNA-binding protein inhibitor ID-1
Class B basic helix-loop-helix protein 24
dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein)
inhibitor of differentiation 1
Inhibitor of DNA binding 1
inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
ID-1a is a negative regulator of helix-loop-helix (HLH) DNA binding proteins. ID-1a contains a HLH motif but no DNA binding motif, therefore, upon binding other HLH proteins, ID-1a acts a dominant negative regulator. ID-1a can be found in both cytoplasmic and nuclear cell fractions. Increased ID-1a expression is associated with cellular proliferation and cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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