ID1 Antibody [Unconjugated]

HAF017). A specific band for ID1 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." > HAF017). A specific band for ID1 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." title="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band for ID1 was detected at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." />

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and MCF-7 human breast cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

ID1 was detected in immersion fixed BG01V human embryonic stem cells, undifferentiated (lower panel) and differentiated into neural progenitor cells (upper panel), using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; <a class=

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ID1 at approximately 30 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." > NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." title="ID1 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative) using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Nuclear and cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." />

ID1 was detected in immersion fixed PC‑3 human prostate cancer cells (Positive) & absent in Daudi human Burkitt's lymphoma cells (Negative) using Goat Anti-Human/Mouse ID1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ID1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse ID1
  Met1-Glu135
  Accession # P20067
• Specificity: Detects human and mouse ID1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ID1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ID1 Antibody [Unconjugated]

• BHLHB24
• bHLHb24DNA-binding protein inhibitor ID-1
• Class B basic helix-loop-helix protein 24
• dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helixprotein)
• dJ857M17.1.2
• ID
• ID1
• inhibitor of differentiation 1
• Inhibitor of DNA binding 1
• inhibitor of DNA binding 1, dominant negative helix-loop-helix protein

Background

ID-1a is a negative regulator of helix-loop-helix (HLH) DNA binding proteins. ID-1a contains a HLH motif but no DNA binding motif, therefore, upon binding other HLH proteins, ID-1a acts a dominant negative regulator. ID-1a can be found in both cytoplasmic and nuclear cell fractions. Increased ID-1a expression is associated with cellular proliferation and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ID1 Antibody (AF4377)(4)

Publications using AF4377

• C Viallard, C Audiger, N Popovic, N Akla, K Lanthier, I Legault-Na, H Melichar, S Costantino, S Lesage, B Larrivée BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 2020-02-10;0(0):. 2020-02-10 [PMID: 32042114] (Western Blot, Human)
• Zhichao Zhang, Adeel Manaf, Yanjiao Li, Sonia Peña Perez, Rajikala Suganthan, John Arne Dahl et al. Histone Methylations Define Neural Stem/Progenitor Cell Subtypes in the Mouse Subventricular Zone Molecular Neurobiology 2/1/2020 [PMID: 31654318]
• T Przyby?a, M Sakowicz-B, I Maciejewsk, H Bielarczyk, T Pawe?czyk Suppression of ID1 expression in colon cancer cells increases sensitivity to 5-fluorouracil Acta Biochim. Pol., 2017-05-17;0(0):. 2017-05-17 [PMID: 28510612] (Western Blot, Human)
• Purandare AV, McDevitt TM, Wan H Characterization of BMS-911543, a functionally selective small-molecule inhibitor of JAK2. Leukemia, 2011-10-21;26(2):280-8. 2011-10-21 [PMID: 22015772] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ID1
• Uniprot:
  • P20067()