Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, MBA‑MB‑468 human breast cancer cell line, and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of ...read more
Smad1 was detected in immersion fixed paraffin-embedded sections of human breast array using Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) at 15 µg/mL overnight at 4 °C. Tissue ...read more
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and MDA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Smad1 at approximately 66 ...read more
Smad1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human Smad1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2039) at 15 µg/mL ...read more
E. coli-derived recombinant human Smad1 Asn2-Met454 Accession # Q15797
Detects human Smad1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) Smad5 is observed, and less than 5% cross-reactivity rhSmad4 and rhSmad9 is observed.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Smad1 Antibody [Unconjugated]
JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
MAD homolog 1
MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
Mad-related protein 1
mothers against decapentaplegic homolog 1
Mothers against DPP homolog 1
SMAD family member 1SMAD 1
SMAD, mothers against DPP homolog 1
TGF-beta signaling protein 1
transforming growth factor-beta signaling protein 1
Smads are a family of intracellular proteins that transmit transforming
growth factor beta (TGF-beta ) superfamily signals from the cell surface to
the nucleus. Upon signaling by some BMP family members, Smad1 is
phosphorylated resulting in its association with the common-mediator
subunit, Smad4. This heteromeric complex then translocates into the
nucleus to exert transcriptional comodulator activity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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